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| These Vasa-Cre mice may be useful in generating conditional germ cell knockouts in both males and females for studies including infertility, gonadogenesis, gametogenesis, and the assembly, activation, and growth of primordial follicles. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Generation ?+F1 (13-MAY-08) Donating Investigator Diego Castrillon, University of Texas Southwestern Description
Mice hemizygous for this Vasa-Cre transgene are viable and fertile. Transgenic cre activity is directed to male and female germ cells starting at embryonic day (e)15-e18. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequence. In such breedings, occasional hemizygous mice may exhibit variegated cre expression in skin epithelium or global cre expression (<20% incidence). Differential parent-of-origin transgene expression is observed. When the mother harbors Vasa-Cre, virtually all progeny undergo global Cre-mediated recombination, even those that do not inherit the transgene (which may be useful in converting a "floxed" allele to a null while obviating the need to perform additional crosses to remove the transgene). To achieve germ-line specific Cre-mediated recombination in offspring, paternal Vasa-Cre mice should be used. These Vasa-Cre mice may be useful in generating conditional germ cell knockouts in both males and females for studies including infertility, gonadogenesis, gametogenesis, and the assembly, activation, and growth of primordial follicles.While another germ line cre-expressing strain, ZP3-Cre (see Stock No. 003651), permits recombination/deletion of loxP-flanked genes in growing follicles, Vasa-Cre mice have additional cre expression in primordial follicles (early oogenesis) and in the male germline.
Development
The Vasa-Cre transgene contains a 5.6 kb fragment of the mouse Vasa (Ddx4) promoter, beta-globin intron, Cre recombinase open reading frame with nuclear localization signal, and SV40 polyadenylation signal. The transgene was microinjected into FVB oocytes. Transgenic mice (founder line not provided) on the FVB genetic background were bred together for many generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (125 strains)
Additional Web Information
Cre-lox Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Developmental Biology Research (Cre-lox System)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Tissue/Cell Markers: Cre-lox System)
Genetics Research (Tissue/Cell Markers: primordial germ cell marker)
Reproductive Biology Research (Cre-lox System)
Reproductive Biology Research (male germ cells)
Reproductive Biology Research (oocyte)
Reproductive Biology Research (primordial germ cell marker)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Ddx4-cre)1Dcas | ||
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| Allele Name | transgene insertion 1, Diego Castrillon | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Vasa-Cre; | ||
| Mutation Made By | Diego Castrillon, University of Texas Southwestern | ||
| Strain of Origin | FVB | ||
| Site of Expression | male and female germ cells starting at embryonic day (e)15-e18 | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Ddx4, DEAD (Asp-Glu-Ala-Asp) box polypeptide 4, mouse, laboratory | ||
| Molecular Note | A 5.6kb fragment of the Ddx4 promoter was ligated to the cre open reading frame with an engineered nuclear localization signal. Eight transgenic lines were created but only two possessed expressing in the gonads as early as E15. Cre expression can be maternally inherited but no ectopic activity is detected in adult mice. [MGI Ref ID J:125051] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Ddx4-cre)1Dcas, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Gallardo T; Shirley L; John GB; Castrillon DH. 2007. Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre. Genesis 45(6):413-7. [PubMed: 17551945] [MGI Ref ID J:125051]
Additional References
Tg(Ddx4-cre)1Dcas relatedJohn GB; Gallardo TD; Shirley LJ; Castrillon DH. 2008. Foxo3 is a PI3K-dependent molecular switch controlling the initiation of oocyte growth. Dev Biol 321(1):197-204. [PubMed: 18601916] [MGI Ref ID J:138697]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred together or to wildtype siblings. Mating System Noncarrier x Hemizygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Hemizygous for Tg(Ddx4-cre)1Dcas *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $288.65 Hemizygous for Tg(Ddx4-cre)1Dcas x Noncarrier $288.65 Noncarrier x Hemizygous for Tg(Ddx4-cre)1Dcas
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Hemizygous for Tg(Ddx4-cre)1Dcas *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $375.30 Hemizygous for Tg(Ddx4-cre)1Dcas x Noncarrier $375.30 Noncarrier x Hemizygous for Tg(Ddx4-cre)1Dcas
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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General Terms and Conditions
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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