Strain Name: |
FVB-Tg(Ddx4-cre)1Dcas/J |
|---|---|
Stock Number: |
006954 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Ddx4; cre; Tg(Ddx4-cre)1Dcas; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Diego Castrillon, University of Texas Southwestern Generation ?+F1 (13-MAY-08) Strain Description
Mice hemizygous for this Vasa-Cre transgene are viable and fertile. Transgenic cre activity is directed to male and female germ cells starting at embryonic day (e)15-e18. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequence. In such breedings, occasional hemizygous mice may exhibit variegated cre expression in skin epithelium or global cre expression (<20% incidence). Differential parent-of-origin transgene expression is observed. When the mother harbors Vasa-Cre, virtually all progeny undergo global Cre-mediated recombination, even those that do not inherit the transgene (which may be useful in converting a "floxed" allele to a null while obviating the need to perform additional crosses to remove the transgene). To achieve germ-line specific Cre-mediated recombination in offspring, paternal Vasa-Cre mice should be used. These Vasa-Cre mice may be useful in generating conditional germ cell knockouts in both males and females for studies including infertility, gonadogenesis, gametogenesis, and the assembly, activation, and growth of primordial follicles.While another germ line cre-expressing strain, ZP3-Cre (see Stock No. 003651), permits recombination/deletion of loxP-flanked genes in growing follicles, Vasa-Cre mice have additional cre expression in primordial follicles (early oogenesis) and in the male germline.
Strain Development
The Vasa-Cre transgene contains a 5.6 kb fragment of the mouse Vasa (Ddx4) promoter, beta-globin intron, Cre recombinase open reading frame with nuclear localization signal, and SV40 polyadenylation signal. The transgene was microinjected into FVB oocytes. Transgenic mice (founder line not provided) on the FVB genetic background were bred together for many generations prior to arrival at The Jackson Laboratory.
Gene & Allele Details
| Allele Symbol | Tg(Ddx4-cre)1Dcas | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Diego Castrillon | ||
| Common Name(s) | Vasa-Cre; | ||
| Mutation Made By | Diego Castrillon, University of Texas Southwestern | ||
| Strain of Origin | FVB | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Ddx4, DEAD (Asp-Glu-Ala-Asp) box polypeptide 4, mouse, laboratory | ||
| Molecular Note | A 5.6kb fragment of the Ddx4 promoter was ligated to the cre open reading frame with an engineered nuclear localization signal. Eight transgenic lines were created but only two possessed expressing in the gonads as early as E15. Cre expression can be maternally inherited but no ectopic activity is detected in adult mice. [J:125051] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Ddx4-cre)1Dcas
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, hemizygous mice may be bred together or to wildtype siblings. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (111 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox-System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Developmental Biology Research (Cre-lox system)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
Genetics Research (Tissue/Cell Markers: Cre-lox system)
Genetics Research (Tissue/Cell Markers: primordial germ cell marker)
Reproductive Biology Research (Cre-lox system)
Reproductive Biology Research (male germ cells)
Reproductive Biology Research (oocyte)
Reproductive Biology Research (primordial germ cell marker)
Research Tools
Cre-lox-System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
References
Selected Reference(s)
Gallardo T; Shirley L; John GB; Castrillon DH. 2007. Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre. Genesis 45(6):413-7. [PubMed: 17551945] [J:125051]
Price and Supply Information
| Strain Name: | FVB-Tg(Ddx4-cre)1Dcas/J |
| Stock Number: | 006954 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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