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| These Vasa-Cre mice may be useful in generating conditional germ cell knockouts in both males and females for studies including infertility, gonadogenesis, gametogenesis, and the assembly, activation, and growth of primordial follicles. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 08-MAY-08 Species laboratory mouse Generation ?+N1F7 (19-MAY-11)
Generation DefinitionsDonating Investigator Diego Castrillon, University of Texas Southwestern Description
Mice hemizygous for this Vasa-Cre transgene are viable and fertile. Transgenic cre activity is directed to male and female germ cells starting at embryonic day (e)15-e18. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequence. In such breedings, occasional hemizygous mice may exhibit variegated cre expression in skin epithelium or global cre expression (<20% incidence). Differential parent-of-origin transgene expression is observed. When the mother harbors Vasa-Cre, virtually all progeny undergo global Cre-mediated recombination, even those that do not inherit the transgene (which may be useful in converting a "floxed" allele to a null while obviating the need to perform additional crosses to remove the transgene). To achieve germ-line specific Cre-mediated recombination in offspring, paternal Vasa-Cre mice should be used.In crosses with some floxed alleles, global recombination may occur even when males are used as the Dxd4-Cre carriers. The basis of this "paternal" effect is not known, but may relate to the presence of Cre protein in sperm. This global recombination can occur more frequently with older males. Thus, when paternal-effect global recombination is observed, it is recommended that the youngest available Ddx4-Cre males be used for breeding (ideally 5-6 weeks, but less than 9 weeks of age). Once a male has proven to repeatedly give rise to globally-recombined progeny, he should no longer be used as a breeder.
These Vasa-Cre mice may be useful in generating conditional germ cell knockouts in both males and females for studies including infertility, gonadogenesis, gametogenesis, and the assembly, activation, and growth of primordial follicles.
While another germ line cre-expressing strain, ZP3-Cre (see Stock No. 003651), permits recombination/deletion of loxP-flanked genes in growing follicles, Vasa-Cre mice have additional cre expression in primordial follicles (early oogenesis) and in the male germline.
Development
The Vasa-Cre transgene contains a 5.6 kb fragment of the mouse Vasa (Ddx4) promoter, beta-globin intron, Cre recombinase open reading frame with nuclear localization signal, and SV40 polyadenylation signal. The transgene was microinjected into FVB oocytes. Transgenic mice (founder line not provided) on the FVB genetic background were bred together for many generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (311 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Germline/Embryonic Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: primordial germ cell marker
Reproductive Biology Research
Cre-lox System
male germ cells
oocyte
primordial germ cell marker
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Ddx4-cre)1Dcas | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Diego Castrillon | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Vasa-Cre; | ||
| Mutation Made By | Diego Castrillon, University of Texas Southwestern | ||
| Strain of Origin | FVB | ||
| Site of Expression | male and female germ cells starting at embryonic day (e)15-e18 | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Ddx4, DEAD (Asp-Glu-Ala-Asp) box polypeptide 4, mouse, laboratory | ||
| Driver Note | Ddx4 | ||
| Molecular Note | A 5.6kb fragment of the Ddx4 promoter was ligated to the cre open reading frame with an engineered nuclear localization signal. Eight transgenic lines were created but only two possessed expressing in the gonads as early as E15. Cre expression can be maternally inherited but no ectopic activity is detected in adult mice. [MGI Ref ID J:125051] | ||
| Gene Symbol and Name | Tg(Ddx4-cre)1Dcas, transgene insertion 1, Diego Castrillon | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Vasa-cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Ddx4-Cre), Melt Curve Analysis
Generic Cre Quantitative PCR, QPCR
Tg(Ddx4-cre)1Dcas, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Gallardo T; Shirley L; John GB; Castrillon DH. 2007. Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre. Genesis 45(6):413-7. [PubMed: 17551945] [MGI Ref ID J:125051]
Additional References
Tg(Ddx4-cre)1Dcas relatedAudouard C; Christians E. 2011. Hsp90b1 knockout targeted to male germline: a mouse model for globozoospermia. Fertil Steril :. [PubMed: 21208614] [MGI Ref ID J:168319]
Chi MN; Auriol J; Jegou B; Kontoyiannis DL; Turner JM; de Rooij DG; Morello D. 2011. The RNA-binding protein ELAVL1/HuR is essential for mouse spermatogenesis, acting both at meiotic and postmeiotic stages. Mol Biol Cell 22(16):2875-2885. [PubMed: 21737689] [MGI Ref ID J:175695]
Goertz MJ; Wu Z; Gallardo TD; Hamra FK; Castrillon DH. 2011. Foxo1 is required in mouse spermatogonial stem cells for their maintenance and the initiation of spermatogenesis. J Clin Invest 121(9):3456-66. [PubMed: 21865646] [MGI Ref ID J:178240]
John GB; Gallardo TD; Shirley LJ; Castrillon DH. 2008. Foxo3 is a PI3K-dependent molecular switch controlling the initiation of oocyte growth. Dev Biol 321(1):197-204. [PubMed: 18601916] [MGI Ref ID J:138697]
Laronda MM; Jameson JL. 2011. Sox3 functions in a cell-autonomous manner to regulate spermatogonial differentiation in mice. Endocrinology 152(4):1606-15. [PubMed: 21248142] [MGI Ref ID J:173878]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred together or to wildtype siblings. Mating System Noncarrier x Hemizygote (Female x Male) 08-MAY-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(Ddx4-cre)1Dcas
Pairs /Price (US dollars $) Pair Genotype $321.00 Hemizygous for Tg(Ddx4-cre)1Dcas x Noncarrier $321.00 Noncarrier x Hemizygous for Tg(Ddx4-cre)1Dcas Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(Ddx4-cre)1Dcas
Pairs /Price (US dollars $) Pair Genotype $417.30 Hemizygous for Tg(Ddx4-cre)1Dcas x Noncarrier $417.30 Noncarrier x Hemizygous for Tg(Ddx4-cre)1Dcas Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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