Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Heterozygote x Heterozygote         (Female x Male) Species laboratory mouse Background Strain NOD/Leuven Donor Strain (C57BL/6 x CBA)F1 H2 Haplotype g7 Generation N14+N1F1 (09-MAY-08)   Donating Investigator Chantal Mathieu,   LEGENDO

Appearance
albino
Related Genotype: A/A Tyr^c/Tyr^c

Description
Heterozygous mice are viable, fertile, and phenotypically indistinguishable from wildtype siblings. Homozygous mutant mice are viable but infertile. No VDR mRNA is detected by RT-PCR in samples from the intestine or kidney or from homozygous mutant embryo. Increased expression of CTP27B1 and reduced expression of CYP24A1 and calbindin-D9k is detected by RT-PCR in samples from VDR-deficient kidneys.

Although mice homozygous for this targeted mutation are viable, shortly after weaning they exhibit dysmorphic features including a flat face and short nose, alopecia, growth retardation, and skeletal defects including hypocalcaemia, decreased bone mineral density, widened growth plates with hypomineralization, less trabeculae and thicker osteo seams. Homozygous mutant mice exhibit metabolic imbalances including abnormally high and low levels of 1,25(OH),2D3 and 25(OH)D3, respectively and abnormal cytokine and chemokine profiles.

Homozygous mice exhibit normal pancreatic islet architecture and insulitis severity is similar to NOD wildtype controls. Diabetes onset and incidence in mutant and wildtype mice is similar for both males (mutants 30% vs wildtypes 38%) and females (mutants 69% versus wildtype 70%) by 250 days of age.

Mice homozygous for this mutation may be useful in studies of rickets, alopecia, skeletal homeostasis, intestinal absorption, the role of 1,25(OH),2D3 in the immune system as it relates to T1D protection, and to determine the function of vitamin D3 analogs in pancreatic beta cells.

Development
Vitamin D receptor, Vdr, is located on Chr 15, 97682461-97736330 bp. Vit. D deficiency in humans increases the risk for type I diabetes in genetically predisposed individuals. In the targeting construct, a neomycin cassette replaced a 1.1-kb fragment containing exon 2, which encodes the first of two zinc fingers in the DNA-binding domain. Properly targeted TT2, (C57BL/6 x CBA)F1, ES cell clones were introduced into CD-1 embryos. Mice carrying this mutation were backcrossed for 14 generation to NOD/Leuven prior to sibling mating. Microsatellite analysis confirms Idd alleles 1 through 15 are NOD in origin. In addition, a concentration of Mit markers were tested on Chr 15 to ensure NOD homozygosity because there are several immune and regulatory genes in close proximity to the Vdr gene. In 2007, the T1DR received this strain at generation N14F7 and mated with NOD/ShiLtJ (Stock No. 001976) once prior to sibling mating.

Control Information

	Control
	Wild-type from the colony
	001976 NOD/ShiLtJ
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Vdr

006133

B6.129S4-Vdrtm1Mbd/J

View Strains carrying other alleles of Vdr     (1 strain)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Vdr^tm1Ska/Vdr^tm1Ska

        NOD.Cg-Vdr^tm1Ska/CmatJ

• growth/size phenotype
• postnatal growth retardation (MGI Ref ID J:127787)
  • mice exhibit growth retardation
• homeostasis/metabolism phenotype
• abnormal glucose tolerance (MGI Ref ID J:127787)
  • glucose tolerance is impaired compared to non-NOD mice but the results are identical when compared to NOD mice with wild-type VDR alleles
• hypocalcemia (MGI Ref ID J:127787)
  • hypocalcemia is found with high levels of the active metabolite of vitamin D in the sera of mice
• immune system phenotype
• abnormal T cell number (MGI Ref ID J:127787)
  • there is a significant decrease in the number of double negative (CD4-CD8-) alphabeta T cells found in the spleen compared to littermate controls
• decreased regulatory T cell number (MGI Ref ID J:127787)
  • there are decreased numbers of CD4⁺CD25⁺ T cells found in the spleen, mesenteric lymph nodes and in the thymus
• abnormal chemokine secretion (MGI Ref ID J:127787)
  • activated macrophages make significantly less CCL2 when activated in vitro with LPS
• abnormal dendritic cell differentiation (MGI Ref ID J:127787)
  • thymic and lymph node dendritic cells show defective maturation as indicated by low CD86 expression
• abnormal interleukin-1 secretion (MGI Ref ID J:127787)
  • there is almost a 4-fold reduction in the amount of IL-1 produced by macrophages when activated by LPS in vitro
  • resting macrophages also produce significantly less IL-1
• decreased interleukin-6 secretion (MGI Ref ID J:127787)
  • there is a 2-fold reduction in the amount of IL-6 produced by macrophages when activated by LPS in vitro
  • resting macrophages also produce less IL-6
• increased susceptibility to autoimmune diabetes (MGI Ref ID J:127787)
  • mice develop insulitis and diabetes at the same rate as NOD mice with wild-type VDR alleles
  • the incidence of diabetes by 250 days of age was 50% in males and 67% in females at N10, and 30% in males and 69% in females at N10
  • mean onset of disease for male mice is 163 days and for female mice 142 days
• hematopoietic system phenotype
• abnormal T cell number (MGI Ref ID J:127787)
  • there is a significant decrease in the number of double negative (CD4-CD8-) alphabeta T cells found in the spleen compared to littermate controls
• decreased regulatory T cell number (MGI Ref ID J:127787)
  • there are decreased numbers of CD4⁺CD25⁺ T cells found in the spleen, mesenteric lymph nodes and in the thymus
• abnormal dendritic cell differentiation (MGI Ref ID J:127787)
  • thymic and lymph node dendritic cells show defective maturation as indicated by low CD86 expression
• skeleton phenotype
• decreased bone density (MGI Ref ID J:127787)
  • decreased bone mineral density is observed along with less trabeculae and thicker osteoid seams
• increased long bone epiphyseal plate size (MGI Ref ID J:127787)
  • mice have widened growth plates that are hypomineralized
• skin/coat/nails phenotype
• alopecia (MGI Ref ID J:127787)
  • mice exhibit alopecia

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Dermatology Research
Skin and Hair Texture Defects

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) (Congenics with mutations affecting cytokine production by autoreactive T cells)

Endocrine Deficiency Research
Bone/Bone Marrow Defects
Gastrointestinal Defects
Skin Defects

Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
Growth Factors/Receptors/Cytokines
Immunodeficiency (Inflammatory bowel disease)
Inflammation (Inflammatory bowel disease)

Internal/Organ Research
Kidney Defects

Vdr^tm1Ska related

Dermatology Research
Skin and Hair Texture Defects

Endocrine Deficiency Research
Bone/Bone Marrow Defects
Gastrointestinal Defects
Skin Defects

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Immunodeficiency (Inflammatory bowel disease)
Immunodeficiency
Inflammation (Inflammatory bowel disease)

Internal/Organ Research
Gastrointestinal Defects (colitis)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Vdrtm1Ska
Allele Name		targeted mutation 1, Shigeaki Kato
Allele Type		Targeted (knock-out)
Common Name(s)		Tokyo VDR-KO; VDR KO; VDR-; VDRKO;
Mutation Made By		Shigeaki Kato,   The University of Tokyo, 113-0032
Strain of Origin		(C57BL/6 x CBA)F1
ES Cell Line Name		TT2
ES Cell Line Strain		(C57BL/6 x CBA)F1
Gene Symbol and Name		Vdr, vitamin D receptor
Chromosome		15
Gene Common Name(s)		NR1I1;
Molecular Note		A neomycin resistance cassette replaced 1.1kb of sequence containing exon 2, which encodes the first zinc finger of the DNA binding domain. RT-PCR and Western blot analysis of intestinal tissue from homozygous mice detected the presence of a truncated transcript and protein that appears to use Met 53 in exon 3 as an initiation site. This truncated protein is able to bind ligand but lacks transactivation activity. [MGI Ref ID J:129684] [MGI Ref ID J:42054]

Genotyping

Genotyping Information

Genotyping Protocols

Vdr^tm1Ska, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Gysemans C; van Etten E; Overbergh L; Giulietti A; Eelen G; Waer M; Verstuyf A; Bouillon R; Mathieu C. 2007. UNALTERED DIABETES PRESENTATION IN NOD MICE LACKING THE VITAMIN D RECEPTOR. Diabetes :. [PubMed: 17959935]  [MGI Ref ID J:127787]

Yoshizawa T; Handa Y; Uematsu Y; Takeda S; Sekine K; Yoshihara Y ; Kawakami T ; Arioka K ; Sato H ; Uchiyama Y ; Masushige S ; Fukamizu A ; Matsumoto T ; Kato S. 1997. Mice lacking the vitamin D receptor exhibit impaired bone formation, uterine hypoplasia and growth retardation after weaning. Nat Genet 16(4):391-6. [PubMed: 9241280]  [MGI Ref ID J:42054]

Additional References

Vdr^tm1Ska related

Aihara K; Azuma H; Akaike M; Ikeda Y; Yamashita M; Sudo T; Hayashi H; Yamada Y; Endoh F; Fujimura M; Yoshida T; Yamaguchi H; Hashizume S; Kato M; Yoshimura K; Yamamoto Y; Kato S; Matsumoto T. 2004. Disruption of nuclear vitamin D receptor gene causes enhanced thrombogenicity in mice. J Biol Chem 279(34):35798-802. [PubMed: 15205460]  [MGI Ref ID J:92388]

Bula CM; Huhtakangas J; Olivera C; Bishop JE; Norman AW; Henry HL. 2005. Presence of a truncated form of the vitamin D receptor (VDR) in a strain of VDR-knockout mice. Endocrinology 146(12):5581-6. [PubMed: 16150907]  [MGI Ref ID J:129684]

Burne TH; Johnston AN; McGrath JJ; Mackay-Sim A. 2006. Swimming behaviour and post-swimming activity in Vitamin D receptor knockout mice. Brain Res Bull 69(1):74-8. [PubMed: 16464687]  [MGI Ref ID J:128632]

Burne TH; McGrath JJ; Eyles DW; Mackay-Sim A. 2005. Behavioural characterization of Vitamin D receptor knockout mice. Behav Brain Res 157(2):299-308. [PubMed: 15639181]  [MGI Ref ID J:95324]

Capuano P; Radanovic T; Wagner CA; Bacic D; Kato S; Uchiyama Y; St-Arnoud R; Murer H; Biber J. 2005. Intestinal and renal adaptation to a low-Pi diet of type II NaPi cotransporters in vitamin D receptor- and 1alphaOHase-deficient mice. Am J Physiol Cell Physiol 288(2):C429-34. [PubMed: 15643054]  [MGI Ref ID J:101227]

Davideau JL; Lezot F; Kato S; Bailleul-Forestier I; Berdal A. 2004. Dental alveolar bone defects related to Vitamin D and calcium status. J Steroid Biochem Mol Biol 89-90:615-8. [PubMed: 15225849]  [MGI Ref ID J:91343]

Demay MB; MacDonald PN; Skorija K; Dowd DR; Cianferotti L; Cox M. 2007. Role of the vitamin D receptor in hair follicle biology. J Steroid Biochem Mol Biol 103(3-5):344-6. [PubMed: 17223342]  [MGI Ref ID J:120248]

Endo I; Inoue D; Mitsui T; Umaki Y; Akaike M; Yoshizawa T; Kato S; Matsumoto T. 2003. Deletion of vitamin D receptor gene in mice results in abnormal skeletal muscle development with deregulated expression of myoregulatory transcription factors. Endocrinology 144(12):5138-44. [PubMed: 12959989]  [MGI Ref ID J:87247]

Endres B; Kato S; DeLuca HF. 2000. Metabolism of 1alpha,25-dihydroxyvitamin D(3) in vitamin D receptor-ablated mice in vivo. Biochemistry 39(8):2123-9. [PubMed: 10684662]  [MGI Ref ID J:60623]

Froicu M; Weaver V; Wynn TA; McDowell MA; Welsh JE; Cantorna MT. 2003. A crucial role for the vitamin d receptor in experimental inflammatory bowel diseases. Mol Endocrinol 17(12):2386-92. [PubMed: 14500760]  [MGI Ref ID J:86860]

Inoue Y; Segawa H; Kaneko I; Yamanaka S; Kusano K; Kawakami E; Furutani J; Ito M; Kuwahata M; Saito H; Fukushima N; Kato S; Kanayama HO; Miyamoto K. 2005. Role of the vitamin D receptor in FGF23 action on phosphate metabolism. Biochem J 390(Pt 1):325-31. [PubMed: 15885032]  [MGI Ref ID J:117597]

Johnson LE; DeLuca HF. 2001. Vitamin D receptor null mutant mice fed high levels of calcium are fertile. J Nutr 131(6):1787-91. [PubMed: 11385068]  [MGI Ref ID J:69822]

Kallay E; Bises G; Bajna E; Bieglmayer C; Gerdenitsch W; Steffan I; Kato S; Armbrecht HJ; Cross HS. 2005. Colon-specific regulation of vitamin D hydroxylases--a possible approach for tumor prevention. Carcinogenesis 26(9):1581-9. [PubMed: 15905206]  [MGI Ref ID J:100745]

Kallay E; Pietschmann P; Toyokuni S; Bajna E; Hahn P; Mazzucco K; Bieglmayer C; Kato S; Cross HS. 2001. Characterization of a vitamin D receptor knockout mouse as a model of colorectal hyperproliferation and DNA damage. Carcinogenesis 22(9):1429-35. [PubMed: 11532865]  [MGI Ref ID J:71459]

Kalueff A; Loseva E; Haapasalo H; Rantala I; Keranen J; Lou YR; Minasyan A; Keisala T; Miettinen S; Kuuslahti M; Tuchimaa P. 2006. Thalamic calcification in vitamin D receptor knockout mice. Neuroreport 17(7):717-21. [PubMed: 16641675]  [MGI Ref ID J:108897]

Kalueff AV; Keisala T; Minasyan A; Kuuslahti M; Miettinen S; Tuohimaa P. 2006. Behavioural anomalies in mice evoked by 'Tokyo' disruption of the Vitamin D receptor gene. Neurosci Res 54(4):254-60. [PubMed: 16427152]  [MGI Ref ID J:107222]

Kalueff AV; Keisala T; Minasyan A; Kuuslahti M; Tuohimaa P. 2006. Temporal stability of novelty exploration in mice exposed to different open field tests. Behav Processes 72(1):104-12. [PubMed: 16442749]  [MGI Ref ID J:112776]

Kalueff AV; Lou YR; Laaksi I; Tuohimaa P. 2005. Abnormal behavioral organization of grooming in mice lacking the vitamin D receptor gene. J Neurogenet 19(1):1-24. [PubMed: 16076629]  [MGI Ref ID J:109821]

Kalueff AV; Lou YR; Laaksi I; Tuohimaa P. 2004. Impaired motor performance in mice lacking neurosteroid vitamin D receptors. Brain Res Bull 64(1):25-9. [PubMed: 15275953]  [MGI Ref ID J:101875]

Kalueff AV; Lou YR; Laaksi I; Tuohimaa P. 2004. Increased anxiety in mice lacking vitamin D receptor gene. Neuroreport 15(8):1271-4. [PubMed: 15167547]  [MGI Ref ID J:102263]

Kalueff AV; Minasyan A; Keisala T; Kuuslahti M; Miettinen S; Tuohimaa P. 2006. Increased severity of chemically induced seizures in mice with partially deleted Vitamin D receptor gene. Neurosci Lett 394(1):69-73. [PubMed: 16256271]  [MGI Ref ID J:105794]

Kato S; Takeyama K; Kitanaka S; Murayama A; Sekine K; Yoshizawa T. 1999. In vivo function of VDR in gene expression-VDR knock-out mice. J Steroid Biochem Mol Biol 69(1-6):247-51. [PubMed: 10418998]  [MGI Ref ID J:56034]

Keisala T; Minasyan A; Jarvelin U; Wang J; Hamalainen T; Kalueff AV; Tuohimaa P. 2007. Aberrant nest building and prolactin secretion in vitamin D receptor mutant mice. J Steroid Biochem Mol Biol 104(3-5):269-73. [PubMed: 17467982]  [MGI Ref ID J:122806]

Kinuta K; Tanaka H; Moriwake T; Aya K; Kato S; Seino Y. 2000. Vitamin D is an important factor in estrogen biosynthesis of both female and male gonads. Endocrinology 141(4):1317-24. [PubMed: 10746634]  [MGI Ref ID J:83237]

Kong J; Li XJ; Gavin D; Jiang Y; Li YC. 2002. Targeted expression of human vitamin d receptor in the skin promotes the initiation of the postnatal hair follicle cycle and rescues the alopecia in vitamin d receptor null mice. J Invest Dermatol 118(4):631-8. [PubMed: 11918709]  [MGI Ref ID J:75902]

Lezot F; Descroix V; Mesbah M; Hotton D; Blin C; Papagerakis P; Mauro N; Kato S; MacDougall M; Sharpe P; Berdal A. 2002. Cross-talk between Msx/Dlx homeobox genes and vitamin D during tooth mineralization. Connect Tissue Res 43(2-3):509-14. [PubMed: 12489206]  [MGI Ref ID J:103313]

Li M; Hener P; Zhang Z; Kato S; Metzger D; Chambon P. 2006. Topical vitamin D3 and low-calcemic analogs induce thymic stromal lymphopoietin in mouse keratinocytes and trigger an atopic dermatitis. Proc Natl Acad Sci U S A 103(31):11736-41. [PubMed: 16880407]  [MGI Ref ID J:111828]

Li M; Indra AK; Warot X; Brocard J; Messaddeq N; Kato S; Metzger D; Chambon P. 2000. Skin abnormalities generated by temporally controlled RXRalpha mutations in mouse epidermis. Nature 407(6804):633-6. [PubMed: 11034212]  [MGI Ref ID J:65109]

Masuyama R; Nakaya Y; Katsumata S; Kajita Y; Uehara M; Tanaka S; Sakai A; Kato S; Nakamura T; Suzuki K. 2003. Dietary calcium and phosphorus ratio regulates bone mineralization and turnover in vitamin D receptor knockout mice by affecting intestinal calcium and phosphorus absorption. J Bone Miner Res 18(7):1217-26. [PubMed: 12854831]  [MGI Ref ID J:111474]

Mathieu C; Van Etten E; Gysemans C; Decallonne B; Kato S; Laureys J; Depovere J; Valckx D; Verstuyf A; Bouillon R. 2001. In vitro and in vivo analysis of the immune system of vitamin D receptor knockout mice. J Bone Miner Res 16(11):2057-65. [PubMed: 11697802]  [MGI Ref ID J:112519]

Meehan TF; DeLuca HF. 2002. The vitamin D receptor is necessary for 1alpha,25-dihydroxyvitamin D(3) to suppress experimental autoimmune encephalomyelitis in mice. Arch Biochem Biophys 408(2):200-4. [PubMed: 12464272]  [MGI Ref ID J:80812]

Minasyan A; Keisala T; Lou YR; Kalueff AV; Tuohimaa P. 2007. Neophobia, sensory and cognitive functions, and hedonic responses in vitamin D receptor mutant mice. J Steroid Biochem Mol Biol 104(3-5):274-80. [PubMed: 17482806]  [MGI Ref ID J:122760]

Murayama A; Takeyama K; Kitanaka S; Kodera Y; Kawaguchi Y; Hosoya T; Kato S. 1999. Positive and negative regulations of the renal 25-hydroxyvitamin D3 1alpha-hydroxylase gene by parathyroid hormone, calcitonin, and 1alpha,25(OH)2D3 in intact animals. Endocrinology 140(5):2224-31. [PubMed: 10218975]  [MGI Ref ID J:114260]

Nakagawa K; Sasaki Y; Kato S; Kubodera N; Okano T. 2005. 22-Oxa-1{alpha},25-dihydroxyvitamin D3 inhibits metastasis and angiogenesis in lung cancer. Carcinogenesis 26(6):1044-54. [PubMed: 15718253]  [MGI Ref ID J:98822]

O'Kelly J; Hisatake J; Hisatake Y; Bishop J; Norman A; Koeffler HP. 2002. Normal myelopoiesis but abnormal T lymphocyte responses in vitamin D receptor knockout mice. J Clin Invest 109(8):1091-9. [PubMed: 11956247]  [MGI Ref ID J:76087]

Palmer HG; Anjos-Afonso F; Carmeliet G; Takeda H; Watt FM. 2008. The Vitamin D Receptor Is a Wnt Effector that Controls Hair Follicle Differentiation and Specifies Tumor Type in Adult Epidermis. PLoS ONE 3(1):e1483. [PubMed: 18213391]  [MGI Ref ID J:131616]

Segawa H; Kaneko I; Yamanaka S; Ito M; Kuwahata M; Inoue Y; Kato S; Miyamoto K. 2004. Intestinal Na-P(i) cotransporter adaptation to dietary P(i) content in vitamin D receptor null mice. Am J Physiol Renal Physiol 287(1):F39-47. [PubMed: 14996670]  [MGI Ref ID J:95427]

Song Y; Fleet JC. 2007. Intestinal resistance to 1,25 dihydroxyvitamin D in mice heterozygous for the vitamin D receptor knockout allele. Endocrinology 148(3):1396-402. [PubMed: 17110426]  [MGI Ref ID J:129578]

Song Y; Kato S; Fleet JC. 2003. Vitamin D receptor (VDR) knockout mice reveal VDR-independent regulation of intestinal calcium absorption and ECaC2 and calbindin D9k mRNA. J Nutr 133(2):374-80. [PubMed: 12566470]  [MGI Ref ID J:119318]

Takeda S; Yoshizawa T; Nagai Y; Yamato H; Fukumoto S; Sekine K ; Kato S ; Matsumoto T ; Fujita T. 1999. Stimulation of osteoclast formation by 1,25-dihydroxyvitamin D requires its binding to vitamin D receptor (VDR) in osteoblastic cells: studies using VDR knockout mice. Endocrinology 140(2):1005-8. [PubMed: 9927335]  [MGI Ref ID J:52911]

Takeyama K; Kitanaka S; Sato T; Kobori M; Yanagisawa J; Kato S. 1997. 25-Hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis. Science 277(5333):1827-30. [PubMed: 9295274]  [MGI Ref ID J:43146]

Tanaka H; Seino Y. 2004. Direct action of 1,25-dihydroxyvitamin D on bone: VDRKO bone shows excessive bone formation in normal mineral condition. J Steroid Biochem Mol Biol 89-90:343-5. [PubMed: 15225798]  [MGI Ref ID J:91345]

Van Cromphaut SJ; Dewerchin M; Hoenderop JG; Stockmans I; Van Herck E; Kato S; Bindels RJ; Collen D; Carmeliet P; Bouillon R; Carmeliet G. 2001. Duodenal calcium absorption in vitamin D receptor-knockout mice: functional and molecular aspects. Proc Natl Acad Sci U S A 98(23):13324-9. [PubMed: 11687634]  [MGI Ref ID J:72578]

Wittke A; Chang A; Froicu M; Harandi OF; Weaver V; August A; Paulson RF; Cantorna MT. 2007. Vitamin D receptor expression by the lung micro-environment is required for maximal induction of lung inflammation. Arch Biochem Biophys 460(2):306-13. [PubMed: 17224129]  [MGI Ref ID J:123350]

Wittke A; Weaver V; Mahon BD; August A; Cantorna MT. 2004. Vitamin D receptor-deficient mice fail to develop experimental allergic asthma. J Immunol 173(5):3432-6. [PubMed: 15322208]  [MGI Ref ID J:92710]

Xie Z; Komuves L; Yu QC; Elalieh H; Ng DC; Leary C; Chang S; Crumrine D; Yoshizawa T; Kato S; Bikle DD. 2002. Lack of the vitamin D receptor is associated with reduced epidermal differentiation and hair follicle growth. J Invest Dermatol 118(1):11-6. [PubMed: 11851870]  [MGI Ref ID J:110729]

Yagishita N; Yamamoto Y; Yoshizawa T; Sekine K; Uematsu Y; Murayama H; Nagai Y; Krezel W; Chambon P; Matsumoto T; Kato S. 2001. Aberrant growth plate development in VDR/RXR gamma double null mutant mice. Endocrinology 142(12):5332-41. [PubMed: 11713233]  [MGI Ref ID J:108798]

Zanello LP; Norman AW. 2004. Rapid modulation of osteoblast ion channel responses by 1alpha,25(OH)2-vitamin D3 requires the presence of a functional vitamin D nuclear receptor. Proc Natl Acad Sci U S A 101(6):1589-94. [PubMed: 14757825]  [MGI Ref ID J:88155]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Mating System	Heterozygote x Heterozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Type 1 Diabetes Repository collection.

Control Information

	Control
	Wild-type from the colony
	001976 NOD/ShiLtJ
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.