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| Because the naked cuticle (NKD) genes are considered targets for Wnt/β-catenin signaling, these nkd1lacZ mutant mice (as well as the similar nkd2lacZ mutant strain; Stock No. 006960) may be useful as a reporter for Wnt/β-catenin-dependent transcriptional activity during embryonic development or in adult mice. | |||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation ?N1+F2 (08-JAN-08) Donating Investigator Keith Wharton, UT Southwestern Medical Center Description
Mice homozygous for this nkd1lacZ mutant allele are viable and fertile with slightly reduced mean litter sizes. Northern blot of lung tissue, as well as western blot of embryonic tissue, confirms that the deleted exons (encoding the Dvl-binding domain) are not expressed in homozygous mutants. However, the endogenous promoter directs expression of the internal ribosome entry site-β-galactosidase (IRES-lacZ) fusion gene in a manner that closely mimics the patterns of the endogenous gene mRNAs. Because the naked cuticle (NKD) genes are considered targets for Wnt/β-catenin signaling, these nkd1lacZ mutant mice (as well as the similar nkd2lacZ mutant strain; Stock No. 006960) may be useful as a reporter for Wnt/β-catenin-dependent transcriptional activity during embryonic development or in adult mice.Development
A targeting vector was designed to insert a stop codon into exon 5 at amino acid 91, as well as replace exons 5-10 of the targeted gene with an internal ribosome entry site-β-galactosidase (IRES-lacZ) fusion gene and neomycin cassette. The construct was electroporated into 129SvEv-derived embryonic stem (ES) cells. Correctly targeted ES cells were microinjected into blastocysts, and chimeric offspring were bred to C57BL/6 mice. Heterozygous mutants were bred to generate homozygotes, which were bred together for many generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (175 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Nkd1tm1Kwha/Nkd1+
involves: 129S/SvEv * C57BL/6
- reproductive system phenotype
- *normal* reproductive system phenotype (MGI Ref ID J:122353)
- mice are viable and fertile
Nkd1tm1Kwha/Nkd1tm1Kwha
involves: 129S/SvEv * C57BL/6
- growth/size phenotype
- postnatal growth retardation (MGI Ref ID J:122353)
- 4- to 12-week old pups fed ad libitum show a slight but significant growth retardation (15% in males at 12 weeks, 4.6% in females at 12 weeks) compared to wild-type mice
- reproductive system phenotype
- *normal* reproductive system phenotype (MGI Ref ID J:122353)
- mice are viable and fertile; average litter size is 7.3
- 3-month old males had similar testis-to-body weight as controls and normal spermatogenesis
- craniofacial phenotype
- short nasal bone (MGI Ref ID J:122353)
- nasal bones are slightly shortened relative to controls or homozygotes on the mixed background
- skeleton phenotype
- short nasal bone (MGI Ref ID J:122353)
- nasal bones are slightly shortened relative to controls or homozygotes on the mixed background
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Nkd1tm1Kwha/Nkd1+
involves: 129S/SvEv
- reproductive system phenotype
- *normal* reproductive system phenotype (MGI Ref ID J:122353)
- mice are viable and fertile
Nkd1tm1Kwha/Nkd1tm1Kwha
involves: 129S/SvEv
- reproductive system phenotype
- decreased litter size (MGI Ref ID J:122353)
- mean litter size of homozygous intercrosses is reduced (1 pup) compared to litters observed on mixed background (5.3 pups) or litters of heterozygous intercrosses regardless of genetic background (4.9-7.3 pups)
- reduced fertility (MGI Ref ID J:122353)
- homozygous matings produce fewer litters (6 mating pairs - 1 litter) on 129/SvEv background compared to those of homozygotes on mixed background (6 mating pairs - 19 litters)
- skeleton phenotype
- *normal* skeleton phenotype (MGI Ref ID J:122353)
- skeletons show no consistent differences relative to wild-type in terms of bone mass or morphology
This mouse can be used to support research in many areas including:
Cell Biology Research
Signal Transduction
Research Tools
lacZ Expression
Developmental Biology Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Nkd1tm1Kwha | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Keith Wharton | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | nkd1lacZ; | ||
| Mutation Made By | Keith Wharton, UT Southwestern Medical Center | ||
| Strain of Origin | 129/SvEv | ||
| ES Cell Line Strain | 129 | ||
| Site of Expression | lacZ expression closely mimics the patterns of the endogenous gene. | ||
| Gene Symbol and Name | Nkd1, naked cuticle 1 homolog (Drosophila) | ||
| Chromosome | 8 | ||
| Gene Common Name(s) | 2810434J10Rik; 9030215G15Rik; Naked1; RIKEN cDNA 2810434J10 gene; RIKEN cDNA 9030215G15 gene; | ||
| Molecular Note | A targeting vector was designed to insert a stop codon into exon 5 at amino acid 91, as well as replace exons 5-10 of the targeted gene with an internal ribosome entry site-b-galactosidase (IRES-lacZ) fusion gene and neomycin cassette. The construct was electroporated into 129SvEv-derived embryonic stem (ES) cells. Northern blot analysis of lung tissue and Western blot analysis of embronic tissue in homozygotes confirm lack of normal mRNA expression, while X-gal staining closely mimics dynamic expressionpattern of normal Nkd1 mRNA. [MGI Ref ID J:122353] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Nkd1tm1Kwha, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Zhang S; Cagatay T; Amanai M; Zhang M; Kline J; Castrillon DH; Ashfaq R; Oz OK; Wharton KA Jr. 2007. Viable mice with compound mutations in the Wnt/Dvl pathway antagonists nkd1 and nkd2. Mol Cell Biol 27(12):4454-64. [PubMed: 17438140] [MGI Ref ID J:122353]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice are bred together. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.70 Female or Male Homozygous for Nkd1tm1Kwha *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $311.40 Homozygous for Nkd1tm1Kwha x Homozygous for Nkd1tm1Kwha
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.50 Female or Male Homozygous for Nkd1tm1Kwha *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $404.90 Homozygous for Nkd1tm1Kwha x Homozygous for Nkd1tm1Kwha
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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