Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Chemically Induced Mutation; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N11pN1   Donating Investigator Pat Levitt,   Vanderbilt University

Description
Homozygotes die at approximately embryonic day 17.5 (E17.5), but heterozygotes are viable and fertile. Homozygous embryos are nearly colorless, are approximately half the size of heterozygous and wildtype littermates, and show altered or delayed brain/body development. There may be a vascular defect. Decreased expression of this gene has previously been implicated with cases of schizophrenia.

Development
This mutation was generated by ethylnitrosourea (ENU) mutagenesis in C3H/HeH males. Foundation stock was selected for reduced enzymatic activity of the associated gene. A point mutation (T to A) creates a splicing defect that eliminates exon 8. Mutants were crossed with 101/H and subsequently backcrossed to C57BL/6J for ten generations by the donating laboratory.

Control Information

	Control
	None Available
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Mdh1^am1H/Mdh1⁺

        involves: C3H/HeH

• homeostasis/metabolism phenotype
• abnormal enzyme/coenzyme activity (MGI Ref ID J:21916)
  • decreased enzyme activity

Mdh1^am1H/Mdh1^am1H

        involves: C3H/HeH

• lethality-prenatal/perinatal
• prenatal lethality (MGI Ref ID J:21916)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Metabolism Research
Enzyme Deficiency

Neurobiology Research
Behavioral and Learning Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Mdh1am1H
Allele Name		mutant 1 Harwell
Allele Type		Chemically induced (ENU)
Mutation Made By		Philip Ebert,   Vanderbilt University
Strain of Origin		C3H/HeH
Gene Symbol and Name		Mdh1, malate dehydrogenase 1, NAD (soluble)
Chromosome		11
Gene Common Name(s)		B230377B03Rik; D17921; DNA segment, D17921; MDH-s; MDHA; MDL1; MGC:1375; MOR2; Mdhl; Mor-2; Mor2; RIKEN cDNA B230377B03 gene; malate dehydrogenase, soluble;
Molecular Note		This allelic variant was generated by ENU mutagenesis. Enzyme assays suggest that the mutation is a null or severe hypomorph.

Genotyping

Genotyping Information

Genotyping Protocols

Mdh1^am1H, Pyrosequencing

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Ball ST; Moseley HJ; Peters J. 1994. Mor2, supernatant malate dehydrogenase, is linked to wa2 and Hba on mouse chromosome 11 in a region of homology with human chromosome 2p. Genomics 24(2):399-400. [PubMed: 7698769]  [MGI Ref ID J:21916]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintained as a live colony, heterozygous x wildtype (or C57BL/6J) crosses may be used. Homozygotes are embryonic lethal.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	None Available
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Fax: 1-207-288-6150
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Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
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Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

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