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Common Names: R26-M2rtTA knock-in;    
The R26-M2rtTA knock-in allows widespread expression of an optimized form of reverse tetracycline-controlled transactivator (rtTA-M2) protein. This R26-M2rtTA strain may be useful for doxycycline-inducible studies which utilize rtTA/tet-O (tet-on/TRE) models.


Strain Information

Type Congenic; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-OCT-09
Specieslaboratory mouse
GenerationN10F10 (12-DEC-13)
Generation Definitions
Donating InvestigatorDr. Rudolf Jaenisch,   Whitehead Institute (MIT)

Homozygotes are viable, fertile, normal in size and do not display any behavioral abnormalities. These targeted mutant mice have widespread expression of an optimized form of reverse tetracycline-controlled transactivator (rtTA-M2) protein. This R26-M2rtTA strain may be useful for doxycycline-inducible studies which utilize rtTA/tet-O (tet-on/TRE) models.

The R26-M2rtTA targeted mutation has an optimized form of reverse tetracycline controlled transactivator (rtTA-M2) followed by a β-globin intron, polyA signal and PGK-puromycin cassette, all inserted downstream of the Gt(ROSA)26Sor promoter. The targeting vector was electroporated into (C57BL/6 x 129S4Sv/Jae)F1-derived V6.5 embryonic stem (ES) cells. In addition, these ES cells were retargeted to insert an frt-flanked PGK-Neo was into the 3' UTR of the Col1a1 locus. Correctly targeted ES cells were selected and the frt-flanked PGK-Neo in the 3' UTR of the Col1a1 locus was replaced by injecting both a "flip-in plasmid" containing a splice acceptor-double polyA sequence and the tetracycline responsive element (TRE or tetO) upstream of the mouse Oct4 (Pou5f1) cDNA sequence, as well as a Flpe-expressing plasmid to facilitate tetO-Oct4 recombination into the 3' UTR of the Col1a1 locus. The resulting ES cells (now targeted with rtTA-M2 in the Gt(ROSA)26Sor locus and tetO-Oct4 in the Col1a1 locus) were injected into B6D2F1 tetraploid blastocysts. Chimeric mice were bred together for many generations prior to arrival at The Jackson Laboratory (as Stock No. Stock No. 006911). Upon arrival, a subset of mice were backcrossed to C57BL/6J; selecting mice harboring the mutant Gt(ROSA)26Sor allele (not harboring the Col1a1 mutant locus) for at least 5 generations to generate this congenic strain (Stock No. 006965).

Control Information

   000664 C57BL/6J
  Considerations for Choosing Controls

Related Strains

View Strains carrying   Gt(ROSA)26Sortm1(rtTA*M2)Jae     (10 strains)

Strains carrying other alleles of Gt(ROSA)26Sor
002292   129-Gt(ROSA)26Sor/J
006053   129-Gt(ROSA)26Sortm1(CAG-EGFP)Luo/J
006067   129-Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/J
006041   129-Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo/J
013205   129S-Gt(ROSA)26Sortm1(NOTCH3)Sat/Mmjax
003310   129S-Gt(ROSA)26Sortm1Sor/J
013207   129S-Gt(ROSA)26Sortm2(NOTCH3*C455R)Sat/Mmjax
009043   129S-Gt(ROSA)26Sortm3(CAG-luc)Tyj/J
007844   129S4/SvJae-Gt(ROSA)26Sortm2(FLP*)Sor/J
003946   129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J
007689   129S4/SvJaeSor-Gt(ROSA)26Sortm4(attB/attP)Sor/J
017626   B6(Cg)-Gt(ROSA)26Sortm1(CAG-GFP/Eif2c2)Zjh/J
026283   B6(Cg)-Gt(ROSA)26Sortm1(CAG-Trp53*,-EGFP)Medz/J
010633   B6(Cg)-Gt(ROSA)26Sortm1(CAG-taulacZ)Bene/J
024540   B6(Cg)-Gt(ROSA)26Sortm1(Sstr3/GFP)Bky/J
008242   B6(Cg)-Gt(ROSA)26Sortm4(Ikbkb)Rsky/J
007676   B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
006071   B6.129-Gt(ROSA)26Sortm1(CAG-EGFP)Luo/J
007708   B6.129-Gt(ROSA)26Sortm1(HD*103Q)Xwy/J
024512   B6.129-Gt(ROSA)26Sortm1(TMPRSS2/ERG)Key/J
008463   B6.129-Gt(ROSA)26Sortm1(cre/ERT2)Tyj/J
008606   B6.129-Gt(ROSA)26Sortm1Joe/J
006080   B6.129-Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/J
006075   B6.129-Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo/J
011008   B6.129P2(Cg)-Gt(ROSA)26Sortm1(tTA)Roos/J
017492   B6.129P2-Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle/J
009669   B6.129P2-Gt(ROSA)26Sortm1(DTA)Lky/J
008513   B6.129P2-Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/J
013586   B6.129P2-Gt(ROSA)26Sortm1Nik/J
013587   B6.129P2-Gt(ROSA)26Sortm3Nik/J
022367   B6.129S4-Gt(ROSA)26Sortm1(CAG-EGFP/Rpl10a,-birA)Wtp/J
009086   B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/RainJ
003474   B6.129S4-Gt(ROSA)26Sortm1Sor/J
012930   B6.129S4-Gt(ROSA)26Sortm2(FLP*)Sor/J
009044   B6.129S4-Gt(ROSA)26Sortm3(CAG-luc)Tyj/J
007743   B6.129S4-Gt(ROSA)26Sortm3(phiC31*)Sor/J
009673   B6.129S6(C)-Gt(ROSA)26Sortm3(HIF1A*)Kael/J
022626   B6.129S6(SJL)-Gt(ROSA)26Sortm2.1(mix1b-mCherry)Mgn/Mmjax
002192   B6.129S7-Gt(ROSA)26Sor/J
006148   B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J
026294   B6.Cg(129S)-Gt(ROSA)26Sortm2.2Ksvo/J
021071   B6.Cg-Gt(ROSA)26Sortm1(CAG-PA-GFP)Rmpl/J
005670   B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
026930   B6.Cg-Gt(ROSA)26Sortm1.1(CAG-tdTomato,-EGFP)Pjen/J
026931   B6.Cg-Gt(ROSA)26Sortm1.2(CAG-tdTomato,-EGFP)Pjen/J
026932   B6.Cg-Gt(ROSA)26Sortm1.3(CAG-tdTomato,-EGFP)Pjen/J
007914   B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J
007920   B6.Cg-Gt(ROSA)26Sortm2(CAG-EYFP)Hze/J
012567   B6.Cg-Gt(ROSA)26Sortm27.1(CAG-COP4*H134R/tdTomato)Hze/J
007903   B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J
024109   B6.Cg-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J
014648   B6.Cg-Gt(ROSA)26Sortm37(H1/tetO-RNAi:Taz)Arte/ZkhuJ
021188   B6.Cg-Gt(ROSA)26Sortm40.1(CAG-aop3/EGFP)Hze/J
007906   B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J
025106   B6.Cg-Gt(ROSA)26Sortm75.1(CAG-tdTomato*)Hze/J
025109   B6.Cg-Gt(ROSA)26Sortm80.1(CAG-COP4*L132C/EYFP)Hze/J
007909   B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J
007897   B6.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
024179   B6;129-Gt(ROSA)26Sortm1(Actb-T,-GFP)Dalco/J
017455   B6;129-Gt(ROSA)26Sortm1(CAG-COP4*E123T*H134R,-tdTomato)Gfng/J
024857   B6;129-Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh/J
010527   B6;129-Gt(ROSA)26Sortm1(DTA)Mrc/J
016262   B6;129-Gt(ROSA)26Sortm1(Foxo1/GFP)Jke/J
017962   B6;129-Gt(ROSA)26Sortm1(RAC1*)Jkis/J
008883   B6;129-Gt(ROSA)26Sortm1(SNCA*A53T)Djmo/TmdJ
004847   B6;129-Gt(ROSA)26Sortm1(cre/ERT)Nat/J
008516   B6;129-Gt(ROSA)26Sortm1Joe/J
003504   B6;129-Gt(ROSA)26Sortm1Sho/J
021847   B6;129-Gt(ROSA)26Sortm1Ytchn/J
008889   B6;129-Gt(ROSA)26Sortm2(SNCA*119)Djmo/TmdJ
009253   B6;129-Gt(ROSA)26Sortm2Nat/J
004077   B6;129-Gt(ROSA)26Sortm2Sho/J
008886   B6;129-Gt(ROSA)26Sortm3(SNCA*E46K)Djmo/TmdJ
010557   B6;129-Gt(ROSA)26Sortm3(rtTA,tetO-cre/ERT)Nat/J
021429   B6;129-Gt(ROSA)26Sortm4(CAG-GFP*)Nat/J
021039   B6;129-Gt(ROSA)26Sortm5(CAG-Sun1/sfGFP)Nat/J
023843   B6;129-Gt(ROSA)26Sortm6(Fzd6)Nat/J
010523   B6;129P2-Gt(ROSA)26Sortm1(CAG-ALPP)Fawa/J
024708   B6;129P2-Gt(ROSA)26Sortm1(CAG-RABVgp4,-TVA)Arenk/J
002073   B6;129S-Gt(ROSA)26Sor/J
018385   B6;129S-Gt(ROSA)26Sortm1(CAG-COX8A/Dendra2)Dcc/J
022516   B6;129S-Gt(ROSA)26Sortm1(Cdkn1c)Jfpa/J
013206   B6;129S-Gt(ROSA)26Sortm1(NOTCH3*R1031C)Sat/Mmjax
018397   B6;129S-Gt(ROSA)26Sortm1.1(CAG-COX8A/Dendra2)Dcc/J
023139   B6;129S-Gt(ROSA)26Sortm1.1Ksvo/J
024846   B6;129S-Gt(ROSA)26Sortm2.1Ksvo/J
012569   B6;129S-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J
012570   B6;129S-Gt(ROSA)26Sortm34.1(CAG-Syp/tdTomato)Hze/J
012735   B6;129S-Gt(ROSA)26Sortm35.1(CAG-aop3/GFP)Hze/J
014538   B6;129S-Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J
014539   B6;129S-Gt(ROSA)26Sortm39(CAG-hop/EYFP)Hze/J
021875   B6;129S-Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze/J
021876   B6;129S-Gt(ROSA)26Sortm66.1(CAG-tdTomato)Hze/J
024105   B6;129S-Gt(ROSA)26Sortm95.1(CAG-GCaMP6f)Hze/J
003309   B6;129S4-Gt(ROSA)26Sortm1Sor/J
004598   B6;129S4-Gt(ROSA)26Sortm2Dym/J
007670   B6;129S4-Gt(ROSA)26Sortm3(phiC31*)Sor/J
024750   B6;129S4-Gt(ROSA)26Sortm9(EGFP/Rpl10a)Amc/J
023035   B6;129S6-Gt(ROSA)26Sortm1(CAG-tdTomato*,-EGFP*)Ees/J
016999   B6;129S6-Gt(ROSA)26Sortm1(rtTA2S*M2)Whsu/J
007908   B6;129S6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J
007905   B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J
024106   B6;129S6-Gt(ROSA)26Sortm96(CAG-GCaMP6s)Hze/J
026175   B6J.129(B6N)-Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh/J
026179   B6J.129(Cg)-Gt(ROSA)26Sortm1.1(CAG-cas9,-EGFP)Fezh/J
026219   B6N.129-Gt(ROSA)26Sortm1(CAG-CHRM4*,-mCitrine)Ute/J
019101   B6N.129S4(B6)-Gt(ROSA)26Sortm1Sor/CjDswJ
016226   B6N.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/J
019013   B6N.129S6(Cg)-Gt(ROSA)26Sortm2(EGFP/cre)Alj/J
019016   B6N.129S6(Cg)-Gt(ROSA)26Sortm3(CAG-FLPo/ERT2)Alj/J
023537   B6N.129S6-Gt(ROSA)26Sortm1(CAG-tdTomato*,-EGFP*)Ees/J
026220   B6N;129-Gt(ROSA)26Sortm2(CAG-CHRM3*,-mCitrine)Ute/J
026261   B6N;129-Gt(ROSA)26Sortm3(CAG-Chrm3*,-mCitrine)Ute/J
025701   B6N;129S1-Gt(ROSA)26Sortm1(Grem1)Svok/J
019120   BALB/c-Gt(ROSA)26Sortm10(Lmp1)Rsky/J
009670   C.129P2(B6)-Gt(ROSA)26Sortm1(DTA)Lky/J
008603   C.129P2(B6)-Gt(ROSA)26Sortm1(tTA)Roos/J
002955   C.129S7-Gt(ROSA)26Sor/J
025322   C57BL/6-Gt(ROSA)26Sortm1(CAG-MFN2*T105M)Dple/J
007900   C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J
008517   C57BL/6-Gt(ROSA)26Sortm3(CAG-MIR17-92,-EGFP)Rsky/J
012637   C57BL/6-Gt(ROSA)26Sortm5(Map3k14)Rsky/J
012638   C57BL/6-Gt(ROSA)26Sortm6(Map3k14*)Rsky/J
012343   C57BL/6-Gt(ROSA)26Sortm7(Pik3ca*,EGFP)Rsky/J
012352   C57BL/6-Gt(ROSA)26Sortm8(Map2k1*,EGFP)Rsky/J
012361   C57BL/6-Gt(ROSA)26Sortm9(Rac1*,EGFP)Rsky/J
020458   C57BL/6N-Gt(ROSA)26Sortm13(CAG-MYC,-CD2*)Rsky/J
005420   C;129S7 Gt(ROSA)26Sor-Bmp5cfe-se7J/GrsrJ
008040   CBy.B6-Gt(ROSA)26Sortm1(HBEGF)Awai/J
007898   CBy.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
026862   D2.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/SjJ
026863   D2.129S4(B6)-Gt(ROSA)26Sortm1Sor/SjJ
009427   FVB.129S4(B6)-Gt(ROSA)26Sortm1Sor/J
005125   FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J
016977   FVB.129S6-Gt(ROSA)26Sortm1(Pik3ca*H1047R)Egan/J
006206   FVB.129S6-Gt(ROSA)26Sortm2(HIF1A/luc)Kael/J
012429   FVB.Cg-Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/J
010920   FVB;129P2-Gt(ROSA)26Sortm1.1(birA)Mejr/J
016603   NOD.B6-Gt(ROSA)26Sortm1(HBEGF)Awai/DvsJ
013731   STOCK Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle/J
010675   STOCK Gt(ROSA)26Sortm1(CAG-EGFP)Fsh/Mmjax
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
022793   STOCK Gt(ROSA)26Sortm1(LRRK2*R1441C)Djmo/J
023451   STOCK Gt(ROSA)26Sortm1(Luc)Kael Tg(UBC-CCR5,-CD4)19Mnz/J
008159   STOCK Gt(ROSA)26Sortm1(Notch1)Dam/J
005130   STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
018121   STOCK Gt(ROSA)26Sortm1(birA)Mejr Gata4tm3.1Wtp/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
008600   STOCK Gt(ROSA)26Sortm1(tTA)Roos/J
010701   STOCK Gt(ROSA)26Sortm1.1(CAG-EGFP)Fsh/Mmjax
022386   STOCK Gt(ROSA)26Sortm1.1(CAG-EGFP/Rpl10a,-birA)Wtp/J
024858   STOCK Gt(ROSA)26Sortm1.1(CAG-cas9,-EGFP)Fezh/J
025983   STOCK Gt(ROSA)26Sortm1.1(birA)Mejr Mef2ctm1.1Wtp/Mmjax
025977   STOCK Gt(ROSA)26Sortm1.1(birA)Mejr Zfpm2tm1.1Wtp /Mmjax
017596   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#aAhmb/J
017597   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#bAhmb/J
025671   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(tetO-Fgf10)1Jaw/SpdlJ
024746   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Hprttm1(tetO-Dkk1)Spdl Tg(TCF/Lef1-lacZ)34Efu/J
010812   STOCK Gt(ROSA)26Sortm1.2(CAG-EGFP)Fsh/Mmjax
017922   STOCK Gt(ROSA)26Sortm10(ACTB-tdTomato)Luo/J
023898   STOCK Gt(ROSA)26Sortm11.1(Setd5-GFP)Mgn/Mmjax
026570   STOCK Gt(ROSA)26Sortm1Embph/J
018903   STOCK Gt(ROSA)26Sortm2(EGFP/cre)Alj/J
018906   STOCK Gt(ROSA)26Sortm3(CAG-FLPo/ERT2)Alj/J
013124   STOCK Gt(ROSA)26Sortm3(Gli3)Amc/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
009674   STOCK Gt(ROSA)26Sortm4(HIF2A*)Kael/J
024107   STOCK Gt(ROSA)26Sortm5(ACTB-tTA)Luo Igs7tm93.1(tetO-GCaMP6f)Hze/HzeJ
012266   STOCK Gt(ROSA)26Sortm5(ACTB-tTA)Luo/J
017912   STOCK Gt(ROSA)26Sortm6(ACTB-EGFP*,-tdTomato)Luo/J
013123   STOCK Gt(ROSA)26Sortm6(Gli1)Amc/J
017921   STOCK Gt(ROSA)26Sortm7(ACTB-EGFP*)Luo/J
017909   STOCK Gt(ROSA)26Sortm8(ACTB-EGFP*,-tTA2)Luo/J
025982   STOCK Rbpjtm1.1Wtp Gt(ROSA)26Sortm1.1birA)Mejr/Mmjax
007577   STOCK Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
View Strains carrying other alleles of Gt(ROSA)26Sor     (176 strains)

Strains carrying other alleles of rtTA
016567   129S.Cg-Tg(Hoxb7-rtTA*M2)2Cos/J
018070   B6.129S6(SJL)-Ptf1atm3.1(rtTA)Mgn/Mmjax
005670   B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
016997   B6.Cg-Tg(Axin2-rtTA2S*M2)7Cos/J
012418   B6.Cg-Tg(CD68-rtTA2S*M2)3Mpil/Mmjax
014098   B6.Cg-Tg(GFAP-rtTA*M2)1Rmra/J
007176   B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes/J
006235   B6.Cg-Tg(SFTPC-rtTA)5Jaw/J
006232   B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
012433   B6;C3-Tg(ACTA1-rtTA,tetO-cre)102Monk/J
021187   B6;FVB-Tg(Pbsn-rtTA*M2)42Xy/J
010574   B6;SJL-Tg(Gh1-rtTA)4-3Jek/J
007678   B6;SJL-Tg(KRT14-rtTA)208Jek/J
010576   B6;SJL-Tg(MMTV-rtTA)4-1Jek/J
010549   B6N.Cg-Tg(Prkcd-glc-1-rtTA)2And/J
016532   B6N.FVB(Cg)-Tg(CAG-rtTA3)4288Slowe/J
006245   C.Cg-Tg(SFTPC-rtTA)5Jaw/J
006242   C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
017955   C57BL/6-Tg(Gfap-rtTA,tetO-MAOB,-lacZ)1Jkan/J
008099   FVB-Tg(KRT14-rtTA)F42Efu/J
008326   FVB-Tg(Pomc-rtTA)1Rck/J
006225   FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J
006222   FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
008202   FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J
004127   FVB/N-Tg(Nes-rtTA)306Rvs/J
006875   FVB/N-Tg(Tagln-rtTA)E1Jwst/J
004602   NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ
005734   NOD/Lt-Tg(Ins2-rtTA)1Ach/AchJ
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
016116   STOCK Waptm2(rtTA)Kuw/J
003273   STOCK Tg(CMV-rtTA)4Bjd/J
018156   STOCK Tg(Drd1a-rtTA)ARgmk/J
019110   STOCK Tg(Hoxb7-rtTA*M2)RS40BCos/Mmjax
008755   STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J
008250   STOCK Tg(Ins2-rtTA)2Efr/J
017519   STOCK Tg(KRT5-rtTA)T2D6Sgkd/J
016146   STOCK Tg(SFTPC-rtTA)2Jaw/J
016145   STOCK Tg(Scgb1a1-rtTA)2Jaw/J
005493   STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J
026194   STOCK Tg(Wnt1-rtTA2S*M2)1Whsu/J
View Strains carrying other alleles of rtTA     (40 strains)

Additional Web Information

Information about the Rosa26 locus on the Soriano lab web page

Tet Expression Systems


Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Tet Expression System
      tTA/rtTA Expressing Strains

Research Tools
Cancer Research
      Tetop Tet System
Cardiovascular Research
      Tetop Tet System
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Tetop Tet System
Neurobiology Research
      Tetop Tet System
Reproductive Biology Research
      Tetop Tet System
Tet Expression Systems
      tTA/rtTA Expressing Strains

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol Gt(ROSA)26Sortm1(rtTA*M2)Jae
Allele Name targeted mutation 1, Rudolf Jaenisch
Allele Type Targeted (Transactivator)
Common Name(s) Gt(ROSA)26Sortm1(M2rtTA)Jae; M2-rtTA; R26-M2rtTA; R26-rtTA; Rosa26-rtRA-nls;
Mutation Made ByDr. Rudolf Jaenisch,   Whitehead Institute (MIT)
Strain of Origin(C57BL/6 x 129S4/SvJae)F1
ES Cell Line Namev6.5
ES Cell Line Strain(C57BL/6 x 129S4/SvJae)F1
Site of ExpressionExpresses an optimized rtTA protein (rtTA-M2). Inducible target gene expression is detected in liver, bone marrow, stomach, intestine, and skin, with lower levels in the heart, lungs, kidney, spleen, and thymus; no expression is detected in the brain and testes.
Expressed Gene rtTA, reverse tetracycline-controlled transactivator, E. coli
The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16).
General Note This mutation was originally created downstream of the Col1a1tm2(tetO-Pou5f1)Jae allele in mutant ES cell line KH2. The two mutations have subsequently been bred apart.
Molecular Note An optimized form of reverse tetracycline controlled transactivator (rtTA-M2) was inserted downstream of the Gt(ROSA)26Sor promoter and was followed by a PGK-puro selection cassette. This mutant form of rtTA termed M2 has five amino acid substitutions inthe tetR moiety of tTA: S12G, E19G, A56P, D148E and H179R. This mutated form of transactivatory protein has increased doxycycline sensitivity. Mice have widespread expression of the rtTA-M2 protein. [MGI Ref ID J:98920]
Gene Symbol and Name Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano
Chromosome 6
Gene Common Name(s) AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; SETD5-AS1; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26;


Genotyping Information

Genotyping Protocols

Gt(rosa)26sorTm1Sor, Probe
Gt(ROSA)26Sortm1sor STD, Separated MCA
Gt(ROSA)26Sortm1sor STD, Standard PCR

Helpful Links

Genotyping resources and troubleshooting


References provided by MGI

Selected Reference(s)

Hochedlinger K; Yamada Y; Beard C; Jaenisch R. 2005. Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues. Cell 121(3):465-77. [PubMed: 15882627]  [MGI Ref ID J:98920]

Additional References

Gt(ROSA)26Sortm1(rtTA*M2)Jae related

Abad M; Mosteiro L; Pantoja C; Canamero M; Rayon T; Ors I; Grana O; Megias D; Dominguez O; Martinez D; Manzanares M; Ortega S; Serrano M. 2013. Reprogramming in vivo produces teratomas and iPS cells with totipotency features. Nature :. [PubMed: 24025773]  [MGI Ref ID J:201192]

Aoki H; Hara A; Era T; Kunisada T; Yamada Y. 2012. Genetic ablation of Rest leads to in vitro-specific derepression of neuronal genes during neurogenesis. Development 139(4):667-77. [PubMed: 22241837]  [MGI Ref ID J:181188]

Beard C; Hochedlinger K; Plath K; Wutz A; Jaenisch R. 2006. Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells. Genesis 44(1):23-8. [PubMed: 16400644]  [MGI Ref ID J:159351]

Boquoi A; Arora S; Chen T; Litwin S; Koh J; Enders GH. 2015. Reversible cell cycle inhibition and premature aging features imposed by conditional expression of p16Ink4a. Aging Cell 14(1):139-47. [PubMed: 25481981]  [MGI Ref ID J:220759]

Bowles J; Secker G; Nguyen C; Kazenwadel J; Truong V; Frampton E; Curtis C; Skoczylas R; Davidson TL; Miura N; Hong YK; Koopman P; Harvey NL; Francois M. 2014. Control of retinoid levels by CYP26B1 is important for lymphatic vascular development in the mouse embryo. Dev Biol 386(1):25-33. [PubMed: 24361262]  [MGI Ref ID J:206581]

Bozec A; Bakiri L; Jimenez M; Rosen ED; Catala-Lehnen P; Schinke T; Schett G; Amling M; Wagner EF. 2013. Osteoblast-specific expression of Fra-2/AP-1 controls adiponectin and osteocalcin expression and affects metabolism. J Cell Sci 126(Pt 23):5432-40. [PubMed: 24046454]  [MGI Ref ID J:211215]

Brambrink T; Foreman R; Welstead GG; Lengner CJ; Wernig M; Suh H; Jaenisch R. 2008. Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2(2):151-9. [PubMed: 18371436]  [MGI Ref ID J:149805]

Brennand K; Huangfu D; Melton D. 2007. All beta Cells Contribute Equally to Islet Growth and Maintenance. PLoS Biol 5(7):e163. [PubMed: 17535113]  [MGI Ref ID J:124045]

Camargo FD; Gokhale S; Johnnidis JB; Fu D; Bell GW; Jaenisch R; Brummelkamp TR. 2007. YAP1 increases organ size and expands undifferentiated progenitor cells. Curr Biol 17(23):2054-60. [PubMed: 17980593]  [MGI Ref ID J:141457]

Carey BW; Markoulaki S; Beard C; Hanna J; Jaenisch R. 2010. Single-gene transgenic mouse strains for reprogramming adult somatic cells. Nat Methods 7(1):56-9. [PubMed: 20010831]  [MGI Ref ID J:157298]

Di Stefano B; Sardina JL; van Oevelen C; Collombet S; Kallin EM; Vicent GP; Lu J; Thieffry D; Beato M; Graf T. 2014. C/EBPalpha poises B cells for rapid reprogramming into induced pluripotent stem cells. Nature 506(7487):235-9. [PubMed: 24336202]  [MGI Ref ID J:207220]

Dickins RA; McJunkin K; Hernando E; Premsrirut PK; Krizhanovsky V; Burgess DJ; Kim SY; Cordon-Cardo C; Zender L; Hannon GJ; Lowe SW. 2007. Tissue-specific and reversible RNA interference in transgenic mice. Nat Genet 39(7):914-21. [PubMed: 17572676]  [MGI Ref ID J:123006]

Dumont-Lagace M; Brochu S; St-Pierre C; Perreault C. 2014. Adult thymic epithelium contains nonsenescent label-retaining cells. J Immunol 192(5):2219-26. [PubMed: 24477909]  [MGI Ref ID J:209820]

Foudi A; Hochedlinger K; Van Buren D; Schindler JW; Jaenisch R; Carey V; Hock H. 2009. Analysis of histone 2B-GFP retention reveals slowly cycling hematopoietic stem cells. Nat Biotechnol 27(1):84-90. [PubMed: 19060879]  [MGI Ref ID J:172421]

Foudi A; Kramer DJ; Qin J; Ye D; Behlich AS; Mordecai S; Preffer FI; Amzallag A; Ramaswamy S; Hochedlinger K; Orkin SH; Hock H. 2014. Distinct, strict requirements for Gfi-1b in adult bone marrow red cell and platelet generation. J Exp Med :. [PubMed: 24711581]  [MGI Ref ID J:207446]

Gros J; Hu JK; Vinegoni C; Feruglio PF; Weissleder R; Tabin CJ. 2010. WNT5A/JNK and FGF/MAPK pathways regulate the cellular events shaping the vertebrate limb bud. Curr Biol 20(22):1993-2002. [PubMed: 21055947]  [MGI Ref ID J:166897]

Guo S; Bai H; Megyola CM; Halene S; Krause DS; Scadden DT; Lu J. 2012. Complex oncogene dependence in microRNA-125a-induced myeloproliferative neoplasms. Proc Natl Acad Sci U S A 109(41):16636-41. [PubMed: 23012470]  [MGI Ref ID J:190324]

Hasenfuss SC; Bakiri L; Thomsen MK; Hamacher R; Wagner EF. 2014. Activator Protein 1 transcription factor Fos-related antigen 1 (Fra-1) is dispensable for murine liver fibrosis, but modulates xenobiotic metabolism. Hepatology 59(1):261-73. [PubMed: 23703832]  [MGI Ref ID J:213764]

Hasenfuss SC; Bakiri L; Thomsen MK; Williams EG; Auwerx J; Wagner EF. 2014. Regulation of steatohepatitis and PPARgamma signaling by distinct AP-1 dimers. Cell Metab 19(1):84-95. [PubMed: 24411941]  [MGI Ref ID J:210545]

He S; Kim I; Lim MS; Morrison SJ. 2011. Sox17 expression confers self-renewal potential and fetal stem cell characteristics upon adult hematopoietic progenitors. Genes Dev 25(15):1613-27. [PubMed: 21828271]  [MGI Ref ID J:174416]

Heinonen KM; Vanegas JR; Brochu S; Shan J; Vainio SJ; Perreault C. 2011. Wnt4 regulates thymic cellularity through the expansion of thymic epithelial cells and early thymic progenitors. Blood 118(19):5163-73. [PubMed: 21937690]  [MGI Ref ID J:178890]

Hirata A; Utikal J; Yamashita S; Aoki H; Watanabe A; Yamamoto T; Okano H; Bardeesy N; Kunisada T; Ushijima T; Hara A; Jaenisch R; Hochedlinger K; Yamada Y. 2013. Dose-dependent roles for canonical Wnt signalling in de novo crypt formation and cell cycle properties of the colonic epithelium. Development 140(1):66-75. [PubMed: 23222438]  [MGI Ref ID J:191050]

Ho R; Papp B; Hoffman JA; Merrill BJ; Plath K. 2013. Stage-specific regulation of reprogramming to induced pluripotent stem cells by Wnt signaling and T cell factor proteins. Cell Rep 3(6):2113-26. [PubMed: 23791530]  [MGI Ref ID J:199370]

Holl D; Kuckenberg P; Woynecki T; Egert A; Becker A; Huss S; Stabenow D; Zimmer A; Knolle P; Tolba R; Fischer HP; Schorle H. 2011. Transgenic Overexpression of Tcfap2c/AP-2gamma Results in Liver Failure and Intestinal Dysplasia. PLoS One 6(7):e22034. [PubMed: 21779369]  [MGI Ref ID J:174049]

Jaako P; Flygare J; Olsson K; Quere R; Ehinger M; Henson A; Ellis S; Schambach A; Baum C; Richter J; Larsson J; Bryder D; Karlsson S. 2011. Mice with ribosomal protein S19 deficiency develop bone marrow failure and symptoms like patients with Diamond-Blackfan anemia. Blood 118(23):6087-96. [PubMed: 21989989]  [MGI Ref ID J:179085]

Jackson MF; Li N; Rodgers BD. 2014. Myostatin regulates tissue potency and cardiac calcium-handling proteins. Endocrinology 155(5):1771-85. [PubMed: 24517228]  [MGI Ref ID J:210483]

Jansson L; Larsson J. 2012. Normal hematopoietic stem cell function in mice with enforced expression of the Hippo signaling effector YAP1. PLoS One 7(2):e32013. [PubMed: 22363786]  [MGI Ref ID J:185310]

Jarde T; Evans RJ; McQuillan KL; Parry L; Feng GJ; Alvares B; Clarke AR; Dale TC. 2013. In vivo and in vitro models for the therapeutic targeting of Wnt signaling using a Tet-ODeltaN89beta-catenin system. Oncogene 32(7):883-93. [PubMed: 22469981]  [MGI Ref ID J:193365]

Kats LM; Reschke M; Taulli R; Pozdnyakova O; Burgess K; Bhargava P; Straley K; Karnik R; Meissner A; Small D; Su SM; Yen K; Zhang J; Pandolfi PP. 2014. Proto-oncogenic role of mutant IDH2 in leukemia initiation and maintenance. Cell Stem Cell 14(3):329-41. [PubMed: 24440599]  [MGI Ref ID J:210097]

Kharas MG; Lengner CJ; Al-Shahrour F; Bullinger L; Ball B; Zaidi S; Morgan K; Tam W; Paktinat M; Okabe R; Gozo M; Einhorn W; Lane SW; Scholl C; Frohling S; Fleming M; Ebert BL; Gilliland DG; Jaenisch R; Daley GQ. 2010. Musashi-2 regulates normal hematopoiesis and promotes aggressive myeloid leukemia. Nat Med 16(8):903-8. [PubMed: 20616797]  [MGI Ref ID J:163322]

Kono M; Tucker AE; Tran J; Bergner JB; Turner EM; Proia RL. 2014. Sphingosine-1-phosphate receptor 1 reporter mice reveal receptor activation sites in vivo. J Clin Invest 124(5):2076-86. [PubMed: 24667638]  [MGI Ref ID J:212772]

Kuzmichev AN; Kim SK; D'Alessio AC; Chenoweth JG; Wittko IM; Campanati L; McKay RD. 2012. Sox2 acts through Sox21 to regulate transcription in pluripotent and differentiated cells. Curr Biol 22(18):1705-10. [PubMed: 22902753]  [MGI Ref ID J:199754]

Laplante M; Horvat S; Festuccia WT; Birsoy K; Prevorsek Z; Efeyan A; Sabatini DM. 2012. DEPTOR Cell-Autonomously Promotes Adipogenesis, and Its Expression Is Associated with Obesity. Cell Metab 16(2):202-12. [PubMed: 22883231]  [MGI Ref ID J:187378]

Leitner NR; Lassnig C; Rom R; Heider S; Bago-Horvath Z; Eferl R; Muller S; Kolbe T; Kenner L; Rulicke T; Strobl B; Muller M. 2014. Inducible, dose-adjustable and time-restricted reconstitution of STAT1 deficiency in vivo. PLoS One 9(1):e86608. [PubMed: 24489749]  [MGI Ref ID J:212694]

Licht T; Dor-Wollman T; Ben-Zvi A; Rothe G; Keshet E. 2015. Vessel maturation schedule determines vulnerability to neuronal injuries of prematurity. J Clin Invest 125(3):1319-28. [PubMed: 25689256]  [MGI Ref ID J:220538]

Linhart HG; Lin H; Yamada Y; Moran E; Steine EJ; Gokhale S; Lo G; Cantu E; Ehrich M; He T; Meissner A; Jaenisch R. 2007. Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing. Genes Dev 21(23):3110-22. [PubMed: 18056424]  [MGI Ref ID J:127808]

Lopez ME; Klein AD; Dimbil UJ; Scott MP. 2011. Anatomically defined neuron-based rescue of neurodegenerative niemann-pick type C disorder. J Neurosci 31(12):4367-78. [PubMed: 21430138]  [MGI Ref ID J:170312]

Lopez ME; Klein AD; Hong J; Dimbil UJ; Scott MP. 2012. Neuronal and epithelial cell rescue resolves chronic systemic inflammation in the lipid storage disorder Niemann-Pick C. Hum Mol Genet 21(13):2946-60. [PubMed: 22493001]  [MGI Ref ID J:184610]

Magnusson M; Brun AC; Miyake N; Larsson J; Ehinger M; Bjornsson JM; Wutz A; Sigvardsson M; Karlsson S. 2007. HOXA10 is a critical regulator for hematopoietic stem cells and erythroid/megakaryocyte development. Blood 109(9):3687-96. [PubMed: 17234739]  [MGI Ref ID J:145322]

Markoulaki S; Hanna J; Beard C; Carey BW; Cheng AW; Lengner CJ; Dausman JA; Fu D; Gao Q; Wu S; Cassady JP; Jaenisch R. 2009. Transgenic mice with defined combinations of drug-inducible reprogramming factors. Nat Biotechnol 27(2):169-71. [PubMed: 19151700]  [MGI Ref ID J:158492]

McJunkin K; Mazurek A; Premsrirut PK; Zuber J; Dow LE; Simon J; Stillman B; Lowe SW. 2011. Reversible suppression of an essential gene in adult mice using transgenic RNA interference. Proc Natl Acad Sci U S A 108(17):7113-8. [PubMed: 21482754]  [MGI Ref ID J:171348]

Nakada D; Oguro H; Levi BP; Ryan N; Kitano A; Saitoh Y; Takeichi M; Wendt GR; Morrison SJ. 2014. Oestrogen increases haematopoietic stem-cell self-renewal in females and during pregnancy. Nature 505(7484):555-8. [PubMed: 24451543]  [MGI Ref ID J:206862]

Ohnishi K; Semi K; Yamamoto T; Shimizu M; Tanaka A; Mitsunaga K; Okita K; Osafune K; Arioka Y; Maeda T; Soejima H; Moriwaki H; Yamanaka S; Woltjen K; Yamada Y. 2014. Premature termination of reprogramming in vivo leads to cancer development through altered epigenetic regulation. Cell 156(4):663-77. [PubMed: 24529372]  [MGI Ref ID J:212039]

Paliwal P; Conboy IM. 2011. Inhibitors of tyrosine phosphatases and apoptosis reprogram lineage-marked differentiated muscle to myogenic progenitor cells. Chem Biol 18(9):1153-66. [PubMed: 21944754]  [MGI Ref ID J:190199]

Petrelli A; Carvello M; Vergani A; Lee KM; Tezza S; Du M; Kleffel S; Chengwen L; Mfarrej BG; Hwu P; Secchi A; Leonard WJ; Young D; Sayegh MH; Markmann JF; Zajac AJ; Fiorina P. 2011. IL-21 is an antitolerogenic cytokine of the late-phase alloimmune response. Diabetes 60(12):3223-34. [PubMed: 22013017]  [MGI Ref ID J:191409]

Premsrirut PK; Dow LE; Kim SY; Camiolo M; Malone CD; Miething C; Scuoppo C; Zuber J; Dickins RA; Kogan SC; Shroyer KR; Sordella R; Hannon GJ; Lowe SW. 2011. A rapid and scalable system for studying gene function in mice using conditional RNA interference. Cell 145(1):145-58. [PubMed: 21458673]  [MGI Ref ID J:171191]

Pruitt SC; Freeland A; Rusiniak ME; Kunnev D; Cady GK. 2013. Cdkn1b overexpression in adult mice alters the balance between genome and tissue ageing. Nat Commun 4:2626. [PubMed: 24149709]  [MGI Ref ID J:221313]

Quere R; Andradottir S; Brun AC; Zubarev RA; Karlsson G; Olsson K; Magnusson M; Cammenga J; Karlsson S. 2011. High levels of the adhesion molecule CD44 on leukemic cells generate acute myeloid leukemia relapse after withdrawal of the initial transforming event. Leukemia 25(3):515-26. [PubMed: 21116281]  [MGI Ref ID J:170242]

Sciammas R; Li Y; Warmflash A; Song Y; Dinner AR; Singh H. 2011. An incoherent regulatory network architecture that orchestrates B cell diversification in response to antigen signaling. Mol Syst Biol 7:495. [PubMed: 21613984]  [MGI Ref ID J:203903]

Sehrawat S; Koenig PA; Kirak O; Schlieker C; Fankhauser M; Ploegh HL. 2013. A catalytically inactive mutant of the deubiquitylase YOD-1 enhances antigen cross-presentation. Blood 121(7):1145-56. [PubMed: 23243279]  [MGI Ref ID J:194608]

Shinoda G; De Soysa TY; Seligson MT; Yabuuchi A; Fujiwara Y; Huang PY; Hagan JP; Gregory RI; Moss EG; Daley GQ. 2013. Lin28a regulates germ cell pool size and fertility. Stem Cells 31(5):1001-9. [PubMed: 23378032]  [MGI Ref ID J:196313]

Stadtfeld M; Maherali N; Borkent M; Hochedlinger K. 2010. A reprogrammable mouse strain from gene-targeted embryonic stem cells. Nat Methods 7(1):53-5. [PubMed: 20010832]  [MGI Ref ID J:159350]

Takiguchi M; Dow LE; Prier JE; Carmichael CL; Kile BT; Turner SJ; Lowe SW; Huang DC; Dickins RA. 2013. Variability of inducible expression across the hematopoietic system of tetracycline transactivator transgenic mice. PLoS One 8(1):e54009. [PubMed: 23326559]  [MGI Ref ID J:195871]

Taylor A; Tang W; Bruscia EM; Zhang PX; Lin A; Gaines P; Wu D; Halene S. 2014. SRF is required for neutrophil migration in response to inflammation. Blood 123(19):3027-36. [PubMed: 24574460]  [MGI Ref ID J:210744]

Tiberi L; Bonnefont J; van den Ameele J; Le Bon SD; Herpoel A; Bilheu A; Baron BW; Vanderhaeghen P. 2014. A BCL6/BCOR/SIRT1 complex triggers neurogenesis and suppresses medulloblastoma by repressing Sonic Hedgehog signaling. Cancer Cell 26(6):797-812. [PubMed: 25490446]  [MGI Ref ID J:217685]

Tokarsky-Amiel R; Azazmeh N; Helman A; Stein Y; Hassan A; Maly A; Ben-Porath I. 2013. Dynamics of Senescent Cell Formation and Retention Revealed by p14ARF Induction in the Epidermis. Cancer Res 73(9):2829-39. [PubMed: 23423975]  [MGI Ref ID J:196969]

Tumaneng K; Schlegelmilch K; Russell RC; Yimlamai D; Basnet H; Mahadevan N; Fitamant J; Bardeesy N; Camargo FD; Guan KL. 2012. YAP mediates crosstalk between the Hippo and PI(3)K-TOR pathways by suppressing PTEN via miR-29. Nat Cell Biol 14(12):1322-9. [PubMed: 23143395]  [MGI Ref ID J:195253]

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Wan M; Kaundal R; Huang H; Zhao J; Yang X; Chaiyachati BH; Li S; Chi T. 2013. A general approach for controlling transcription and probing epigenetic mechanisms: application to the CD4 locus. J Immunol 190(2):737-47. [PubMed: 23293358]  [MGI Ref ID J:191711]

Watanabe S; Hirai H; Asakura Y; Tastad C; Verma M; Keller C; Dutton JR; Asakura A. 2011. MyoD gene suppression by Oct4 is required for reprogramming in myoblasts to produce induced pluripotent stem cells. Stem Cells 29(3):505-16. [PubMed: 21425413]  [MGI Ref ID J:175957]

Wesemann DR; Portuguese AJ; Magee JM; Gallagher MP; Zhou X; Panchakshari RA; Alt FW. 2012. Reprogramming IgH isotype-switched B cells to functional-grade induced pluripotent stem cells. Proc Natl Acad Sci U S A 109(34):13745-50. [PubMed: 22869756]  [MGI Ref ID J:188595]

Yamada K; Ohno T; Aoki H; Semi K; Watanabe A; Moritake H; Shiozawa S; Kunisada T; Kobayashi Y; Toguchida J; Shimizu K; Hara A; Yamada Y. 2013. EWS/ATF1 expression induces sarcomas from neural crest-derived cells in mice. J Clin Invest :. [PubMed: 23281395]  [MGI Ref ID J:194505]

Yamada Y; Aoki H; Kunisada T; Hara A. 2010. Rest promotes the early differentiation of mouse ESCs but is not required for their maintenance. Cell Stem Cell 6(1):10-5. [PubMed: 20085738]  [MGI Ref ID J:157076]

Yu H; Suleiman H; Kim AH; Miner JH; Dani A; Shaw AS; Akilesh S. 2013. Rac1 activation in podocytes induces rapid foot process effacement and proteinuria. Mol Cell Biol 33(23):4755-64. [PubMed: 24061480]  [MGI Ref ID J:206088]

Zhu H; Shah S; Shyh-Chang N; Shinoda G; Einhorn WS; Viswanathan SR; Takeuchi A; Grasemann C; Rinn JL; Lopez MF; Hirschhorn JN; Palmert MR; Daley GQ. 2010. Lin28a transgenic mice manifest size and puberty phenotypes identified in human genetic association studies. Nat Genet 42(7):626-30. [PubMed: 20512147]  [MGI Ref ID J:166568]

Zhu H; Shyh-Chang N; Segre AV; Shinoda G; Shah SP; Einhorn WS; Takeuchi A; Engreitz JM; Hagan JP; Kharas MG; Urbach A; Thornton JE; Triboulet R; Gregory RI; Altshuler D; Daley GQ. 2011. The Lin28/let-7 Axis Regulates Glucose Metabolism. Cell 147(1):81-94. [PubMed: 21962509]  [MGI Ref ID J:177113]

Zong Y; Huang J; Sankarasharma D; Morikawa T; Fukayama M; Epstein JI; Chada KK; Witte ON. 2012. Stromal epigenetic dysregulation is sufficient to initiate mouse prostate cancer via paracrine Wnt signaling. Proc Natl Acad Sci U S A 109(50):E3395-404. [PubMed: 23184966]  [MGI Ref ID J:193135]

van Amerongen R; Fuerer C; Mizutani M; Nusse R. 2012. Wnt5a can both activate and repress Wnt/beta-catenin signaling during mouse embryonic development. Dev Biol 369(1):101-14. [PubMed: 22771246]  [MGI Ref ID J:187621]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-OCT-09
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $205.90Female or MaleHomozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae  
Price per Pair (US dollars $)Pair Genotype
$411.80Homozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae x Homozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae  

Standard Supply

Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $267.70Female or MaleHomozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae  
Price per Pair (US dollars $)Pair Genotype
$535.40Homozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae x Homozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae  

Standard Supply

Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

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Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty


In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.