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| Targeted mutant mice have widespread expression of an optimized form of reverse tetracycline-controlled transactivator (rtTA-M2) protein. This R26-M2rtTA strain may be useful for doxycycline-inducible studies which utilize rtTA/tet-O (tet-on/TRE) models. | |||||||||||||||
- Description
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Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 01-OCT-09 Species laboratory mouse Generation N10F1 (12-NOV-09) Donating Investigator Rudolf Jaenisch, Whitehead Institute (MIT) Description
Homozygotes are viable, fertile, normal in size and do not display any behavioral abnormalities. These targeted mutant mice have widespread expression of an optimized form of reverse tetracycline-controlled transactivator (rtTA-M2) protein. This R26-M2rtTA strain may be useful for doxycycline-inducible studies which utilize rtTA/tet-O (tet-on/TRE) models.Development
Embryonic stem (ES) cells from the (C57BL/6 x 129S4Sv/Jae)F1-derived V6.5 ES cell line were used to for targeted insertion of an optimized form of reverse tetracycline controlled transactivator (rtTA-M2) downstream of the Gt(ROSA)26Sor promoter. In addition, the 3' UTR region of the Col1a1 locus was modified for Frt-mediated targeting. Correctly targeted ES cells were then injected with a "flip-in plasmid" containing a tetracycline responsive element (TRE or tetO) and mouse Oct4 (Pou5f1) cDNA sequence (and Flpe-recombinase plasmid to facilitate tetO-Oct4 integration into the 3' UTR region of Col1a1). The resulting ES cells were injected into B6D2F1 tetraploid blastocysts. Chimeric mice were bred together for many generations prior to arrival at The Jackson Laboratory (as Stock No. Stock No. 006911). Upon arrival, a subset of mice were backcrossed to C57BL/6J; selecting mice with the mutant Gt(ROSA)26Sor allele (and against the other mutant locus) for at least 5 generations to generate this congenic strain (Stock No. 006965).
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Gt(ROSA)26Sortm1(rtTA*M2)Jae allele
006911 B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J View Strains carrying Gt(ROSA)26Sortm1(rtTA*M2)Jae (1 strain)
005670 B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 007176 B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes/J 006235 B6.Cg-Tg(SFTPC-rtTA)5Jaw/J 006232 B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 007678 B6;SJL-Tg(KRT14-rtTA)208Jek/J 010576 B6;SJL-Tg(MMTV-rtTA)4-1Jek/J 006245 C.Cg-Tg(SFTPC-rtTA)5Jaw/J 006242 C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 008099 FVB-Tg(KRT14-rtTA)F42Efu/J 004127 FVB-Tg(Nes-rtTA)306Rvs/J 006225 FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J 006222 FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 008202 FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J 006875 FVB/N-Tg(Tagln-rtTA)E1Jwst/J 004602 NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ 005734 NOD/Lt-Tg(Ins2-rtTA)1Ach/AchJ 005572 STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 008755 STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J 008250 STOCK Tg(Ins2-rtTA)2Efr/J 005493 STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J 003273 STOCK Tg(rtTAhCMV)4Bjd/J
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Neurobiology Research
Tet Expression System
tTA/rtTA Expressing Strains
Research Tools
Cancer Research
Tetop Tet System
Cardiovascular Research
Tetop Tet System
Genetics Research
Mutagenesis and Transgenesis: Tetop Tet System
Neurobiology Research
Tetop Tet System
Reproductive Biology Research
Tetop Tet System
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm1(rtTA*M2)Jae | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Rudolf Jaenisch | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Gt(ROSA)26Sortm1(M2rtTA)Jae; R26-M2rtTA; R26-rtTA; | ||
| Mutation Made By | Rudolf Jaenisch, Whitehead Institute (MIT) | ||
| Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 | ||
| ES Cell Line Name | v6.5 | ||
| ES Cell Line Strain | (C57BL/6 x 129S4/SvJae)F1 | ||
| Site of Expression | Expresses an optimized rtTA protein (rtTA-M2). Inducible target gene expression is detected in liver, bone marrow, stomach, intestine, and skin, with lower levels in the heart, lungs, kidney, spleen, and thymus; no expression is detected in the brain and testes. | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| General Note |
This mutation is always used in combination with the Col1a1 | ||
| Molecular Note | An optimized form of reverse tetracycline controlled transactivator (rtTA-M2) was inserted downstream of the Gt(ROSA)26Sor promoter. This mutant form of rtTA termed M2 has five amino acid substitutions in the tetR moiety of tTA: S12G, E19G, A56P, D148E and H179R. This mutated form of transactivatory protein has increased doxycycline sensitivity. Mice have widespread expression of the rtTA-M2 protein. Doxycycline treatment induces expression of Pou5f1 from the Col1a1 locus under the control of a tetracycline-responsive element in these mice. [MGI Ref ID J:98920] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1sor STD, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Hochedlinger K; Yamada Y; Beard C; Jaenisch R. 2005. Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues. Cell 121(3):465-77. [PubMed: 15882627] [MGI Ref ID J:98920]
Additional References
Gt(ROSA)26Sortm1(rtTA*M2)Jae relatedBrennand K; Huangfu D; Melton D. 2007. All beta Cells Contribute Equally to Islet Growth and Maintenance. PLoS Biol 5(7):e163. [PubMed: 17535113] [MGI Ref ID J:124045]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Mating System Homozygote x Homozygote (Female x Male) 01-OCT-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $160.40 Female or Male Homozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae
Pairs /Price (US dollars $) Pair Genotype $320.80 Homozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae x Homozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $208.60 Female or Male Homozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae
Pairs /Price (US dollars $) Pair Genotype $417.10 Homozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae x Homozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
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