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| When these Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in widespread cells/tissues. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.Cg-Tg(UBC-cre/ESR1)1Ejb/J (Changed: 20-JUN-08 ) Type Congenic; Mutant Stock; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 23-APR-08 Species laboratory mouse Generation N10F (18-NOV-09)
Generation DefinitionsDonating Investigator Eric Brown, Univ of Penn - Abramson Inst (AFCRI) Description
Mice hemizygous for this Cre-ERT2 transgene are viable and fertile. Mice from this founder line have strong tamoxifen-inducible cre activity in all reported tissue types. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to OHT. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in widespread cells/tissues.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
This Cre-ERT2 transgenic mouse line was generated via lentitransgenesis; a self-inactivating lentiviral vector was designed with the human ubiquitin C (UBC) promoter sequence upstream of a Cre-ERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain). Cre-ERT2-expressing lentivirus were microinjected into the perivitelline space of one-cell zygotes (129SvEv;C57BL/6 mixed genetic background). Morula and blastocyst stage embryos were implanted into pseudopregnant recipient mice. A founder with a single-copy integrant expressing high levels of Cre-ERT2 was chosen to establish the Cre-ERT2 lentitransgenic mouse line. These mice may be on a mixed C57BL/6;129S genetic background.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 101043 B6129SF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(UBC-cre/ESR1)1Ejb allele
007179 129S.Cg-Tg(UBC-cre/ESR1)1Ejb/J 008085 B6.Cg-Tg(UBC-cre/ESR1)1Ejb/J View Strains carrying Tg(UBC-cre/ESR1)1Ejb (2 strains)
007179 129S.Cg-Tg(UBC-cre/ESR1)1Ejb/J 006259 B6.Cg-Pepcb Ptprca Tg(UBC-scFv)2Nemz/J 008085 B6.Cg-Tg(UBC-cre/ESR1)1Ejb/J 004353 C57BL/6-Tg(UBC-GFP)30Scha/J 007076 CByJ.B6-Tg(UBC-GFP)30Scha/J
007179 129S.Cg-Tg(UBC-cre/ESR1)1Ejb/J 008085 B6.Cg-Tg(UBC-cre/ESR1)1Ejb/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression: Germline/Embryonic Expression
Cre Recombinase Expression: Inducible
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(UBC-cre/ESR1)1Ejb | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Eric J Brown | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Tg(UBC-cre)1Ejb; Ubc-cre; | ||
| Mutation Made By | Eric Brown, Univ of Penn - Abramson Inst (AFCRI) | ||
| Strain of Origin | (129S/SvEv x C57BL/6) | ||
| Site of Expression | tamoxifen-inducible cre activity in all tested tissues | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | UBC, ubiquitin C, human | ||
| Driver Note | UBC | ||
| Inducible Note | induced by tamoxifen | ||
| Molecular Note | This Cre-ERT2 transgenic mouse line was generated via lentitransgenesis; a self-inactivating lentiviral vector was designed with the human ubiquitin C (UBC) promoter sequence upstream of a Cre-ERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain). Cre-ERT2-expressing lentivirus were microinjected into the perivitelline space of one-cell zygotes (129SvEv;C57BL/6 mixed genetic background). Mice display tamoxifen-inducible Cre activity in all tissue types. [MGI Ref ID J:123200] | ||
| Gene Symbol and Name | Tg(UBC-cre/ESR1)1Ejb, transgene insertion 1, Eric J Brown | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg(UBC-cre)1Ejb; Ubc-cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Fast MCA
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre Quantitative PCR, QPCR
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Ruzankina Y; Pinzon-Guzman C; Asare A; Ong T; Pontano L; Cotsarelis G; Zediak VP; Velez M; Bhandoola A; Brown EJ. 2007. Deletion of the developmentally essential gene ATR in adult mice leads to age-related phenotypes and stem cell loss. Cell Stem Cell 1(1):113-26. [PubMed: 18371340] [MGI Ref ID J:123200]
Additional References
Tg(UBC-cre/ESR1)1Ejb relatedGruber M; Hu CJ; Johnson RS; Brown EJ; Keith B; Simon MC. 2007. Acute postnatal ablation of Hif-2alpha results in anemia. Proc Natl Acad Sci U S A 104(7):2301-6. [PubMed: 17284606] [MGI Ref ID J:119731]
Lum JJ; Bui T; Gruber M; Gordan JD; Deberardinis RJ; Covello KL; Simon MC; Thompson CB. 2007. The transcription factor HIF-1{alpha} plays a critical role in the growth factor-dependent regulation of both aerobic and anaerobic glycolysis. Genes Dev 21(9):1037-49. [PubMed: 17437992] [MGI Ref ID J:121261]
Maillard I ; Chen YX ; Friedman A ; Yang Y ; Tubbs AT ; Shestova O ; Pear WS ; Hua X. 2009. Menin regulates the function of hematopoietic stem cells and lymphoid progenitors. Blood 113(8):1661-9. [PubMed: 19228930] [MGI Ref ID J:146598]
O'Driscoll M. 2009. Mouse models for ATR deficiency. DNA Repair (Amst) 8(11):1333-7. [PubMed: 19782648] [MGI Ref ID J:153964]
Ruzankina Y; Schoppy DW; Asare A; Clark CE; Vonderheide RH; Brown EJ. 2009. Tissue regenerative delays and synthetic lethality in adult mice after combined deletion of Atr and Trp53. Nat Genet 41(10):1144-9. [PubMed: 19718024] [MGI Ref ID J:155013]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic mice can be bred to wildtype siblings. Attempts to breed hemi x hemi mice yielded no homozygous animals as determined by PCR (Jul-2008). Mating System Noncarrier x Hemizygote (Female x Male) 23-APR-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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 |
Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(UBC-cre/ESR1)1Ejb
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(UBC-cre/ESR1)1Ejb x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(UBC-cre/ESR1)1Ejb Standard Supply
Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and/or larger quantities of mice may be possible. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes
- Usually shipped between four and eight weeks of age.
| Pricing for International shipping destinations |
|
|
Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(UBC-cre/ESR1)1Ejb
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(UBC-cre/ESR1)1Ejb x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(UBC-cre/ESR1)1Ejb Standard Supply
Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and/or larger quantities of mice may be possible. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes
- Usually shipped between four and eight weeks of age.
|
|
|
Standard Supply
Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and/or larger quantities of mice may be possible. Estimated ship dates for all orders provided within two business days following order placement.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 101043 B6129SF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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