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| When these Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in widespread cells/tissues. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names B6;129S-Tg(UBC-cre/ESR1)1Ejb/J (Changed: 30-MAR-10 ) B6.Cg-Tg(UBC-cre/ESR1)1Ejb/J (Changed: 20-JUN-08 ) Type Congenic; Mutant Stock; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 23-APR-08 Species laboratory mouse Generation N10F6 (05-DEC-11)
Generation DefinitionsDonating Investigator Eric Brown, Univ of Penn - Abramson Inst (AFCRI) Description
Mice hemizygous for this Cre-ERT2 transgene are viable and fertile. Mice from this founder line have strong tamoxifen-inducible cre activity in all reported tissue types. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to OHT. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in widespread cells/tissues.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
This Cre-ERT2 transgenic mouse line was generated via lentitransgenesis; a self-inactivating lentiviral vector was designed with the human ubiquitin C (UBC) promoter sequence upstream of a Cre-ERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain). Cre-ERT2-expressing lentivirus were microinjected into the perivitelline space of one-cell zygotes (129SvEv;C57BL/6 mixed genetic background). Morula and blastocyst stage embryos were implanted into pseudopregnant recipient mice. A founder with a single-copy integrant expressing high levels of Cre-ERT2 was chosen to establish the Cre-ERT2 lentitransgenic mouse line. These mice are on a mixed C57BL/6;129S genetic background.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 101043 B6129SF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(UBC-cre/ERT2)1Ejb allele
007179 129S.Cg-Tg(UBC-cre/ERT2)1Ejb/J 008085 B6.Cg-Tg(UBC-cre/ERT2)1Ejb/J View Strains carrying Tg(UBC-cre/ERT2)1Ejb (2 strains)
017614 B6(Cg)-Tyrc-2J Tg(UBC-mCherry)1Phbs/J 006259 B6.Cg-Pepcb Ptprca Tg(UBC-scFv)2Nemz/J 013115 B6.Cg-Rag1tm1Mom Tg(UBC-GFP)30Scha/J 015805 B6.Cg-Tg(UBC-GFP,-TVA)1Clc/J 015806 B6.Cg-Tg(UBC-GFP,-TVA)2Clc/J 015807 B6.Cg-Tg(UBC-GFP,-TVA)3Clc/J 015808 B6.Cg-Tg(UBC-TVA)1Clc/J 004353 C57BL/6-Tg(UBC-GFP)30Scha/J 007076 CByJ.B6-Tg(UBC-GFP)30Scha/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Estrogen Receptor 1; ESR1 (ESR1)
Migraine with or without Aura, Susceptibility to, 1 (ESR1)
Myocardial Infarction, Susceptibility to (ESR1)
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Germline/Embryonic Expression
Cre Recombinase Expression: Inducible
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(UBC-cre/ERT2)1Ejb | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Eric J Brown | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Cre-ER; Cre-ERT2; CreT2; Tg(UBC-cre)1Ejb; Tg(UBC-cre/ESR1)1Ejb; UBC-CreERt2; Ubc-cre; Ubc-cre/ERT2; Ubc-creER; | ||
| Mutation Made By | Eric Brown, Univ of Penn - Abramson Inst (AFCRI) | ||
| Strain of Origin | (129S/SvEv x C57BL/6) | ||
| Site of Expression | tamoxifen-inducible cre activity in all tested tissues | ||
| Expressed Gene | ESR1, estrogen receptor 1, human | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | UBC, ubiquitin C, human | ||
| Driver Note | UBC | ||
| Inducible Note | induced by tamoxifen | ||
| Molecular Note | This Cre-ERT2 transgenic mouse line was generated via lentitransgenesis; a self-inactivating lentiviral vector was designed with the human ubiquitin C (UBC) promoter sequence upstream of a Cre-ERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain). Cre-ERT2-expressing lentivirus were microinjected into the perivitelline space of one-cell zygotes (129SvEv;C57BL/6 mixed genetic background). Mice display tamoxifen-inducible Cre activity in all tissue types. [MGI Ref ID J:123200] | ||
| Gene Symbol and Name | Tg(UBC-cre/ERT2)1Ejb, transgene insertion 1, Eric J Brown | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg(UBC-cre)1Ejb; Tg(UBC-cre/ESR1)1Ejb; Ubc-cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre Quantitative PCR, QPCR
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Ruzankina Y; Pinzon-Guzman C; Asare A; Ong T; Pontano L; Cotsarelis G; Zediak VP; Velez M; Bhandoola A; Brown EJ. 2007. Deletion of the developmentally essential gene ATR in adult mice leads to age-related phenotypes and stem cell loss. Cell Stem Cell 1(1):113-26. [PubMed: 18371340] [MGI Ref ID J:123200]
Additional References
Tg(UBC-cre/ERT2)1Ejb relatedBauler TJ; Kamiya N; Lapinski PE; Langewisch E; Mishina Y; Wilkinson JE; Feng GS; King PD. 2011. Development of severe skeletal defects in induced SHP-2-deficient adult mice: a model of skeletal malformation in humans with SHP-2 mutations. Dis Model Mech 4(2):228-39. [PubMed: 21068439] [MGI Ref ID J:169264]
Bebee TW; Gladman JT; Chandler DS. 2011. Generation of a tamoxifen inducible SMN mouse for temporal SMN replacement. Genesis 49(12):927-34. [PubMed: 21538807] [MGI Ref ID J:185111]
Bezman NA; Cedars E; Steiner DF; Blelloch R; Hesslein DG; Lanier LL. 2010. Distinct requirements of microRNAs in NK cell activation, survival, and function. J Immunol 185(7):3835-46. [PubMed: 20805417] [MGI Ref ID J:164317]
Chang J; Burkett PR; Borges CM; Kuchroo VK; Turka LA; Chang CH. 2013. MyD88 is essential to sustain mTOR activation necessary to promote T helper 17 cell proliferation by linking IL-1 and IL-23 signaling. Proc Natl Acad Sci U S A 110(6):2270-5. [PubMed: 23341605] [MGI Ref ID J:193828]
Chen YX; Yan J; Keeshan K; Tubbs AT; Wang H; Silva A; Brown EJ; Hess JL; Pear WS; Hua X. 2006. The tumor suppressor menin regulates hematopoiesis and myeloid transformation by influencing Hox gene expression. Proc Natl Acad Sci U S A 103(4):1018-23. [PubMed: 16415155] [MGI Ref ID J:105708]
Corbo-Rodgers E; Staub ES; Zou T; Smith A; Kambayashi T; Maltzman JS. 2012. Oral ivermectin as an unexpected initiator of CreT2-mediated deletion in T cells. Nat Immunol 13(3):197-8. [PubMed: 22344272] [MGI Ref ID J:181643]
Corbo-Rodgers E; Wiehagen KR; Staub ES; Maltzman JS. 2012. Homeostatic division is not necessary for antigen-specific CD4+ memory T cell persistence. J Immunol 189(7):3378-85. [PubMed: 22956580] [MGI Ref ID J:190737]
Costa-e-Sousa RH; Astapova I; Ye F; Wondisford FE; Hollenberg AN. 2012. The thyroid axis is regulated by NCoR1 via its actions in the pituitary. Endocrinology 153(10):5049-57. [PubMed: 22878400] [MGI Ref ID J:191436]
Ding L; Morrison SJ. 2013. Haematopoietic stem cells and early lymphoid progenitors occupy distinct bone marrow niches. Nature 495(7440):231-5. [PubMed: 23434755] [MGI Ref ID J:194748]
Ding L; Saunders TL; Enikolopov G; Morrison SJ. 2012. Endothelial and perivascular cells maintain haematopoietic stem cells. Nature 481(7382):457-62. [PubMed: 22281595] [MGI Ref ID J:180431]
Dong Z; Davidson D; Perez-Quintero LA; Kurosaki T; Swat W; Veillette A. 2012. The adaptor SAP controls NK cell activation by regulating the enzymes Vav-1 and SHIP-1 and by enhancing conjugates with target cells. Immunity 36(6):974-85. [PubMed: 22683124] [MGI Ref ID J:187430]
Elorza A; Soro-Arnaiz I; Melendez-Rodriguez F; Rodriguez-Vaello V; Marsboom G; de Carcer G; Acosta-Iborra B; Albacete-Albacete L; Ordonez A; Serrano-Oviedo L; Gimenez-Bachs JM; Vara-Vega A; Salinas A; Sanchez-Prieto R; Martin del Rio R; Sanchez-Madrid F;Malumbres M; Landazuri MO; Aragones J. 2012. HIF2alpha acts as an mTORC1 activator through the amino acid carrier SLC7A5. Mol Cell 48(5):681-91. [PubMed: 23103253] [MGI Ref ID J:194004]
Ganuza M; Saiz-Ladera C; Canamero M; Gomez G; Schneider R; Blasco MA; Pisano D; Paramio JM; Santamaria D; Barbacid M. 2012. Genetic inactivation of Cdk7 leads to cell cycle arrest and induces premature aging due to adult stem cell exhaustion. EMBO J :. [PubMed: 22505032] [MGI Ref ID J:183925]
Gao Y; Sartori DJ; Li C; Yu QC; Kushner JA; Simon MC; Diehl JA. 2012. PERK is required in the adult pancreas and is essential for maintenance of glucose homeostasis. Mol Cell Biol 32(24):5129-39. [PubMed: 23071091] [MGI Ref ID J:192859]
Gilad O; Nabet BY; Ragland RL; Schoppy DW; Smith KD; Durham AC; Brown EJ. 2010. Combining ATR suppression with oncogenic Ras synergistically increases genomic instability, causing synthetic lethality or tumorigenesis in a dosage-dependent manner. Cancer Res 70(23):9693-702. [PubMed: 21098704] [MGI Ref ID J:166996]
Gruber M; Hu CJ; Johnson RS; Brown EJ; Keith B; Simon MC. 2007. Acute postnatal ablation of Hif-2alpha results in anemia. Proc Natl Acad Sci U S A 104(7):2301-6. [PubMed: 17284606] [MGI Ref ID J:119731]
Gruber M; Mathew LK; Runge AC; Garcia JA; Simon MC. 2010. EPAS1 Is Required for Spermatogenesis in the Postnatal Mouse Testis. Biol Reprod 82(6):1227-36. [PubMed: 20181618] [MGI Ref ID J:161980]
Hegde S; Hankey P; Paulson RF. 2012. Self-renewal of leukemia stem cells in friend virus-induced erythroleukemia requires proviral insertional activation of Spi1 and Hedgehog signaling but not mutation of p53. Stem Cells 30(2):121-30. [PubMed: 22083997] [MGI Ref ID J:190190]
Jacobs SR; Michalek RD; Rathmell JC. 2010. IL-7 is essential for homeostatic control of T cell metabolism in vivo. J Immunol 184(7):3461-9. [PubMed: 20194717] [MGI Ref ID J:160083]
Jung H; Hsiung B; Pestal K; Procyk E; Raulet DH. 2012. RAE-1 ligands for the NKG2D receptor are regulated by E2F transcription factors, which control cell cycle entry. J Exp Med 209(13):2409-22. [PubMed: 23166357] [MGI Ref ID J:194086]
Kalaitzidis D; Sykes SM; Wang Z; Punt N; Tang Y; Ragu C; Sinha AU; Lane SW; Souza AL; Clish CB; Anastasiou D; Gilliland DG; Scadden DT; Guertin DA; Armstrong SA. 2012. mTOR complex 1 plays critical roles in hematopoiesis and Pten-loss-evoked leukemogenesis. Cell Stem Cell 11(3):429-39. [PubMed: 22958934] [MGI Ref ID J:193428]
Kobayashi H; Gilbert V; Liu Q; Kapitsinou PP; Unger TL; Rha J; Rivella S; Schlondorff D; Haase VH. 2012. Myeloid cell-derived hypoxia-inducible factor attenuates inflammation in unilateral ureteral obstruction-induced kidney injury. J Immunol 188(10):5106-15. [PubMed: 22490864] [MGI Ref ID J:188688]
Lamming DW; Ye L; Katajisto P; Goncalves MD; Saitoh M; Stevens DM; Davis JG; Salmon AB; Richardson A; Ahima RS; Guertin DA; Sabatini DM; Baur JA. 2012. Rapamycin-induced insulin resistance is mediated by mTORC2 loss and uncoupled from longevity. Science 335(6076):1638-43. [PubMed: 22461615] [MGI Ref ID J:182354]
Liu Q; Davidoff O; Niss K; Haase VH. 2012. Hypoxia-inducible factor regulates hepcidin via erythropoietin-induced erythropoiesis. J Clin Invest 122(12):4635-44. [PubMed: 23114598] [MGI Ref ID J:193997]
Lukaszewicz AI; Anderson DJ. 2011. Cyclin D1 promotes neurogenesis in the developing spinal cord in a cell cycle-independent manner. Proc Natl Acad Sci U S A 108(28):11632-7. [PubMed: 21709239] [MGI Ref ID J:174395]
Lum JJ; Bui T; Gruber M; Gordan JD; Deberardinis RJ; Covello KL; Simon MC; Thompson CB. 2007. The transcription factor HIF-1{alpha} plays a critical role in the growth factor-dependent regulation of both aerobic and anaerobic glycolysis. Genes Dev 21(9):1037-49. [PubMed: 17437992] [MGI Ref ID J:121261]
Maillard I ; Chen YX ; Friedman A ; Yang Y ; Tubbs AT ; Shestova O ; Pear WS ; Hua X. 2009. Menin regulates the function of hematopoietic stem cells and lymphoid progenitors. Blood 113(8):1661-9. [PubMed: 19228930] [MGI Ref ID J:146598]
Matson CK; Murphy MW; Griswold MD; Yoshida S; Bardwell VJ; Zarkower D. 2010. The mammalian doublesex homolog DMRT1 is a transcriptional gatekeeper that controls the mitosis versus meiosis decision in male germ cells. Dev Cell 19(4):612-24. [PubMed: 20951351] [MGI Ref ID J:166057]
Mionnet C; Sanos SL; Mondor I; Jorquera A; Laugier JP; Germain RN; Bajenoff M. 2011. High endothelial venules as traffic control points maintaining lymphocyte population homeostasis in lymph nodes. Blood 118(23):6115-22. [PubMed: 21937697] [MGI Ref ID J:179107]
Miro-Murillo M; Elorza A; Soro-Arnaiz I; Albacete-Albacete L; Ordonez A; Balsa E; Vara-Vega A; Vazquez S; Fuertes E; Fernandez-Criado C; Landazuri MO; Aragones J. 2011. Acute Vhl Gene Inactivation Induces Cardiac HIF-Dependent Erythropoietin Gene Expression. PLoS One 6(7):e22589. [PubMed: 21811636] [MGI Ref ID J:174924]
Miyoshi H; Ajima R; Luo CT; Yamaguchi TP; Stappenbeck TS. 2012. Wnt5a potentiates TGF-beta signaling to promote colonic crypt regeneration after tissue injury. Science 338(6103):108-13. [PubMed: 22956684] [MGI Ref ID J:187747]
Nakada D, Saunders TL, Morrison SJ. 2010. Lkb1 regulates cell cycle and energy metabolism in hematopoietic stem cells Nature 468(7324):653-658. [PubMed: 21124450] [MGI Ref ID J:165090]
O'Driscoll M. 2009. Mouse models for ATR deficiency. DNA Repair (Amst) 8(11):1333-7. [PubMed: 19782648] [MGI Ref ID J:153964]
Ouyang W; Liao W; Luo CT; Yin N; Huse M; Kim MV; Peng M; Chan P; Ma Q; Mo Y; Meijer D; Zhao K; Rudensky AY; Atwal G; Zhang MQ; Li MO. 2012. Novel Foxo1-dependent transcriptional programs control T(reg) cell function. Nature 491(7425):554-9. [PubMed: 23135404] [MGI Ref ID J:189962]
Palazon A; Martinez-Forero I; Teijeira A; Morales-Kastresana A; Alfaro C; Sanmamed MF; Perez-Gracia JL; Penuelas I; Hervas-Stubbs S; Rouzaut A; de Landazuri MO; Jure-Kunkel M; Aragones J; Melero I. 2012. The HIF-1alpha hypoxia response in tumor-infiltrating T lymphocytes induces functional CD137 (4-1BB) for immunotherapy. Cancer Discov 2(7):608-23. [PubMed: 22719018] [MGI Ref ID J:193060]
Popmihajlov Z; Xu D; Morgan H; Milligan Z; Smith KA. 2012. Conditional IL-2 Gene Deletion: Consequences for T Cell Proliferation. Front Immunol 3:102. [PubMed: 22590468] [MGI Ref ID J:190168]
Price NL; Gomes AP; Ling AJ; Duarte FV; Martin-Montalvo A; North BJ; Agarwal B; Ye L; Ramadori G; Teodoro JS; Hubbard BP; Varela AT; Davis JG; Varamini B; Hafner A; Moaddel R; Rolo AP; Coppari R; Palmeira CM; de Cabo R; Baur JA; Sinclair DA. 2012. SIRT1 is required for AMPK activation and the beneficial effects of resveratrol on mitochondrial function. Cell Metab 15(5):675-90. [PubMed: 22560220] [MGI Ref ID J:184593]
Quintana A; Kruse SE; Kapur RP; Sanz E; Palmiter RD. 2010. Complex I deficiency due to loss of Ndufs4 in the brain results in progressive encephalopathy resembling Leigh syndrome. Proc Natl Acad Sci U S A 107(24):10996-1001. [PubMed: 20534480] [MGI Ref ID J:161393]
Rahman AH; Zhang R; Blosser CD; Hou B; Defranco AL; Maltzman JS; Wherry EJ; Turka LA. 2011. Antiviral memory CD8 T-cell differentiation, maintenance, and secondary expansion occur independently of MyD88. Blood 117(11):3123-30. [PubMed: 21233312] [MGI Ref ID J:170518]
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Ruzankina Y; Schoppy DW; Asare A; Clark CE; Vonderheide RH; Brown EJ. 2009. Tissue regenerative delays and synthetic lethality in adult mice after combined deletion of Atr and Trp53. Nat Genet 41(10):1144-9. [PubMed: 19718024] [MGI Ref ID J:155013]
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Schoppy DW; Ragland RL; Gilad O; Shastri N; Peters AA; Murga M; Fernandez-Capetillo O; Diehl JA; Brown EJ. 2012. Oncogenic stress sensitizes murine cancers to hypomorphic suppression of ATR. J Clin Invest 122(1):241-52. [PubMed: 22133876] [MGI Ref ID J:184414]
Smith-Garvin JE; Burns JC; Gohil M; Zou T; Kim JS; Maltzman JS; Wherry EJ; Koretzky GA; Jordan MS. 2010. T-cell receptor signals direct the composition and function of the memory CD8+ T-cell pool. Blood 116(25):5548-59. [PubMed: 20847203] [MGI Ref ID J:167402]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic mice can be bred to wildtype siblings. Attempts to breed hemi x hemi mice yielded no homozygous animals as determined by PCR (Jul-2008). Mating System Noncarrier x Hemizygote (Female x Male) 23-APR-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(UBC-cre/ERT2)1Ejb
Price per Pair (US dollars $) Pair Genotype $302.00 Hemizygous for Tg(UBC-cre/ERT2)1Ejb x Noncarrier $302.00 Noncarrier x Hemizygous for Tg(UBC-cre/ERT2)1Ejb Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(UBC-cre/ERT2)1Ejb
Price per Pair (US dollars $) Pair Genotype $392.60 Hemizygous for Tg(UBC-cre/ERT2)1Ejb x Noncarrier $392.60 Noncarrier x Hemizygous for Tg(UBC-cre/ERT2)1Ejb Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
|
|
|
Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 101043 B6129SF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.