| |||||||||||||||
| When these Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in widespread cells/tissues. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.Cg-Tg(UBC-cre/ESR1)1Ejb/J (Changed: 20-JUN-08 ) Type Congenic; Mutant Stock; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 23-APR-08 Species laboratory mouse Generation N10F (18-NOV-09) Donating Investigator Eric Brown, Univ of Penn - Abramson Inst (AFCRI) Description
Mice hemizygous for this Cre-ERT2 transgene are viable and fertile. Mice from this founder line have strong tamoxifen-inducible cre activity in all reported tissue types. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to OHT. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in widespread cells/tissues.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
This Cre-ERT2 transgenic mouse line was generated via lentitransgenesis; a self-inactivating lentiviral vector was designed with the human ubiquitin C (UBC) promoter sequence upstream of a Cre-ERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain). Cre-ERT2-expressing lentivirus were microinjected into the perivitelline space of one-cell zygotes (129SvEv;C57BL/6 mixed genetic background). Morula and blastocyst stage embryos were implanted into pseudopregnant recipient mice. A founder with a single-copy integrant expressing high levels of Cre-ERT2 was chosen to establish the Cre-ERT2 lentitransgenic mouse line. These mice may be on a mixed C57BL/6;129S genetic background.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 101043 B6129SF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(UBC-cre/ESR1)1Ejb allele
007179 129S.Cg-Tg(UBC-cre/ESR1)1Ejb/J 008085 B6.Cg-Tg(UBC-cre/ESR1)1Ejb/J View Strains carrying Tg(UBC-cre/ESR1)1Ejb (2 strains)
006661 129S.B6-Tg(KRT14-RAF1/ESR1)1Pkha/J 005975 B6.Cg-Tg(Plp1-cre/ESR1)3Pop/J 007606 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J 009614 B6.Cg-Tg(Wfs1-cre/ERT2)2Aibs/J 008529 B6;129P-Tg(Neurog1-cre/ESR1)1Good/J 009103 B6;C3-Tg(Wfs1-cre/ERT2)3Aibs/J 007610 B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 004995 C3H-Tg(Camk2a-Creb1/ESR1)3Sva/J 002712 FVB/N-Tg(PF4MER)6Kra/J 007684 STOCK Tg(Atoh1-cre/ESR1)14Fsh/J 008861 STOCK Tg(Ela1-Cre/ESR1)1Stof/J
006259 B6.Cg-Pepcb Ptprca Tg(UBC-scFv)2Nemz/J 004353 C57BL/6-Tg(UBC-GFP)30Scha/J 007076 CByJ.B6-Tg(UBC-GFP)30Scha/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression: Germline/Embryonic Expression
Cre Recombinase Expression: Inducible
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(UBC-cre/ESR1)1Ejb | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Eric J Brown | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Tg(UBC-cre)1Ejb; Ubc-cre; | ||
| Mutation Made By | Eric Brown, Univ of Penn - Abramson Inst (AFCRI) | ||
| Strain of Origin | (129S/SvEv x C57BL/6) | ||
| Site of Expression | tamoxifen-inducible cre activity in all tested tissues | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Expressed Gene | ESR1, estrogen receptor 1, human | ||
| Promoter | UBC, ubiquitin C, human | ||
| Driver Note | UBC | ||
| Inducible Note | induced by tamoxifen | ||
| Molecular Note | This Cre-ERT2 transgenic mouse line was generated via lentitransgenesis; a self-inactivating lentiviral vector was designed with the human ubiquitin C (UBC) promoter sequence upstream of a Cre-ERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain). Cre-ERT2-expressing lentivirus were microinjected into the perivitelline space of one-cell zygotes (129SvEv;C57BL/6 mixed genetic background). Mice display tamoxifen-inducible Cre activity in all tissue types. [MGI Ref ID J:123200] | ||
| Gene Symbol and Name | Tg(UBC-cre/ESR1)1Ejb, transgene insertion 1, Eric J Brown | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg(UBC-cre)1Ejb; Ubc-cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre Quantitative PCR, QPCR
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Ruzankina Y; Pinzon-Guzman C; Asare A; Ong T; Pontano L; Cotsarelis G; Zediak VP; Velez M; Bhandoola A; Brown EJ. 2007. Deletion of the developmentally essential gene ATR in adult mice leads to age-related phenotypes and stem cell loss. Cell Stem Cell 1(1):113-26. [PubMed: 18371340] [MGI Ref ID J:123200]
Additional References
Tg(UBC-cre/ESR1)1Ejb relatedGruber M; Hu CJ; Johnson RS; Brown EJ; Keith B; Simon MC. 2007. Acute postnatal ablation of Hif-2alpha results in anemia. Proc Natl Acad Sci U S A 104(7):2301-6. [PubMed: 17284606] [MGI Ref ID J:119731]
Lum JJ; Bui T; Gruber M; Gordan JD; Deberardinis RJ; Covello KL; Simon MC; Thompson CB. 2007. The transcription factor HIF-1{alpha} plays a critical role in the growth factor-dependent regulation of both aerobic and anaerobic glycolysis. Genes Dev 21(9):1037-49. [PubMed: 17437992] [MGI Ref ID J:121261]
Maillard I ; Chen YX ; Friedman A ; Yang Y ; Tubbs AT ; Shestova O ; Pear WS ; Hua X. 2009. Menin regulates the function of hematopoietic stem cells and lymphoid progenitors. Blood 113(8):1661-9. [PubMed: 19228930] [MGI Ref ID J:146598]
O'Driscoll M. 2009. Mouse models for ATR deficiency. DNA Repair (Amst) 8(11):1333-7. [PubMed: 19782648] [MGI Ref ID J:153964]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic mice can be bred to wildtype siblings. Attempts to breed hemi x hemi mice yielded no homozygous animals as determined by PCR (Jul-2008). Mating System Noncarrier x Hemizygote (Female x Male) 23-APR-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(UBC-cre/ESR1)1Ejb
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(UBC-cre/ESR1)1Ejb x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(UBC-cre/ESR1)1Ejb
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(UBC-cre/ESR1)1Ejb
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(UBC-cre/ESR1)1Ejb x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(UBC-cre/ESR1)1Ejb
Additional Supply Details
|
|
|
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 101043 B6129SF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.