Strain Name:

B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ

Stock Number:

007004

Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use

Description

Strain Information

Former Names B6.CBA-Tg(Camk2a-tTA)1Mmay/DboJ    (Changed: 28-AUG-07 )
Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   14-APR-08
Specieslaboratory mouse
GenerationN9+N1F3 (08-JAN-09)
 
Donating Investigator David Borchelt,   McKnight Brain Inst, Univ of Florida

Description
Transgenic mice expressing the tetracycline-controlled transactivator protein (tTA) under regulatory control of the forebrain-specific calcium-calmodulin-dependent kinase II (Camk2a) promoter are viable and fertile. When hemizygotes are mated to a second strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene can be blocked by administration of the tetracycline analog, doxycycline (dox). These mice are a "Tet-Off" tool that allow the inducible expression of genes in forebrain neurons, and may be useful in studying brain disorders such as Alzheimer's disease (when used in conjunction with Stock No. 005706, Stock No. 007049, Stock No. 007051, Stock No. 007052), Parkinson's disease, or other neurodegenerative diseases.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development
A transgenic construct was designed with 8.5 kb of the mouse CaMKIIalpha promoter placed upstream of the tetracycline-regulated transactivator (tTA or "Tet-Off") gene (flanked by an artificial intron and splice sites at the 5' end and by a polyadenylation signal from SV40 at the 3' end). Founder line B was established and maintained on a mixed B6;CBA genetic background upon its arrival at The Jackson Laboratory (as Stock No. 003010). These mice were obtained by Dr. David Borchelt (University of Florida) and then backcrossed to C57BL/6J inbred mice for at least 8 generations prior to sending back to The Jackson Laboratory (as Stock No. 007004).

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Tg(Camk2a-tTA)1Mmay allele
003010   B6;CBA-Tg(Camk2a-tTA)1Mmay/J
View Strains carrying   Tg(Camk2a-tTA)1Mmay     (1 strain)

View Strains carrying other alleles of Camk2a     (10 strains)

View Strains carrying other alleles of tTA     (21 strains)

Additional Web Information

Tet Expression Systems
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Alzheimer's Disease
Behavioral and Learning Defects
Parkinson's Disease
Tet Expression System
      tTA/rtTA Expressing Strains

Research Tools
Neurobiology Research
      Tetop Tet System
Tet Expression Systems
      tTA/rtTA Expressing Strains

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Tg(Camk2a-tTA)1Mmay
Allele Name transgene insertion 1, Mark Mayford
Allele Type Transgenic (random, expressed)
Common Name(s) CAMK-rTA; CaMKIIalpha-tTA; CamDAT; Tg(alphacre); Tg(CaMKIItTA)Mmay; Tgalpha-CaMkII-tTA; pCaMKII-tTA;
Mutation Made By Mark Mayford,   The Scripps Research Institute
Site of ExpressionExpresses tTA in forebrain neurons.
Expressed Gene tTA, tetracycline-controlled transactivator, E. coli
The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter.
Promoter Camk2a, calcium/calmodulin-dependent protein kinase II alpha, mouse, laboratory
General Note This transgene is line B.

Transgenic mice are viable, fertile, and display no overt phenotypic defects. Administration of tetracycline analogs such as doxycycline blocks transgene expression.

Molecular Note The transgene contains the tetracycline-controlled transactivator protein (tTA) under regulatory control of the forebrain specific calcium/calmodulin-dependent kinase II promoter. [MGI Ref ID J:37107]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(tTA), Melt Curve Analysis
Tg(tTA), QPCR
Tg(tTA), Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Mayford M; Bach ME; Huang YY; Wang L; Hawkins RD; Kandel ER. 1996. Control of memory formation through regulated expression of a CaMKII transgene. Science 274(5293):1678-83. [PubMed: 8939850]  [MGI Ref ID J:37107]

Additional References

Tg(Camk2a-tTA)1Mmay related

Aleksic T; Baumann B; Wagner M; Adler G; Wirth T; Weber CK. 2007. Cellular immune reaction in the pancreas is induced by constitutively active IkappaB kinase-2. Gut 56(2):227-36. [PubMed: 16870717]  [MGI Ref ID J:130547]

Alvarez-Saavedra M; Saez MA; Kang D; Zoghbi HY; Young JI. 2007. Cell-specific expression of wild-type MeCP2 in mouse models of Rett syndrome yields insight about pathogenesis. Hum Mol Genet 16(19):2315-25. [PubMed: 17635839]  [MGI Ref ID J:124365]

Barco A; Patterson S; Alarcon JM; Gromova P; Mata-Roig M; Morozov A; Kandel ER. 2005. Gene expression profiling of facilitated L-LTP in VP16-CREB mice reveals that BDNF is critical for the maintenance of LTP and its synaptic capture. Neuron 48(1):123-37. [PubMed: 16202713]  [MGI Ref ID J:105355]

Bedford L; Hay D; Devoy A; Paine S; Powe DG; Seth R; Gray T; Topham I; Fone K; Rezvani N; Mee M; Soane T; Layfield R; Sheppard PW; Ebendal T; Usoskin D; Lowe J; Mayer RJ. 2008. Depletion of 26S proteasomes in mouse brain neurons causes neurodegeneration and Lewy-like inclusions resembling human pale bodies. J Neurosci 28(33):8189-98. [PubMed: 18701681]  [MGI Ref ID J:138991]

Bejar R; Yasuda R; Krugers H; Hood K; Mayford M. 2002. Transgenic calmodulin-dependent protein kinase II activation: dose-dependent effects on synaptic plasticity, learning, and memory. J Neurosci 22(13):5719-26. [PubMed: 12097524]  [MGI Ref ID J:112443]

Chandrasekaran K; Hazelton JL; Wang Y; Fiskum G; Kristian T. 2006. Neuron-specific conditional expression of a mitochondrially targeted fluorescent protein in mice. J Neurosci 26(51):13123-7. [PubMed: 17182763]  [MGI Ref ID J:118452]

Chen AP; Ohno M; Giese KP; Kuhn R; Chen RL; Silva AJ. 2006. Forebrain-specific knockout of B-raf kinase leads to deficits in hippocampal long-term potentiation, learning, and memory. J Neurosci Res 83(1):28-38. [PubMed: 16342120]  [MGI Ref ID J:107042]

Chen L; Ding Y; Cagniard B; Van Laar AD; Mortimer A; Chi W; Hastings TG; Kang UJ; Zhuang X. 2008. Unregulated cytosolic dopamine causes neurodegeneration associated with oxidative stress in mice. J Neurosci 28(2):425-33. [PubMed: 18184785]  [MGI Ref ID J:131093]

Cheng HY; Dziema H; Papp J; Mathur DP; Koletar M; Ralph MR; Penninger JM; Obrietan K. 2006. The molecular gatekeeper Dexras1 sculpts the photic responsiveness of the mammalian circadian clock. J Neurosci 26(50):12984-95. [PubMed: 17167088]  [MGI Ref ID J:116668]

Cruz JC; Tseng HC; Goldman JA; Shih H; Tsai LH. 2003. Aberrant Cdk5 activation by p25 triggers pathological events leading to neurodegeneration and neurofibrillary tangles. Neuron 40(3):471-83. [PubMed: 14642273]  [MGI Ref ID J:104240]

Diaz-Hernandez M; Diez-Zaera M; Sanchez-Nogueiro J; Gomez-Villafuertes R; Canals JM; Alberch J; Miras-Portugal MT; Lucas JJ. 2009. Altered P2X7-receptor level and function in mouse models of Huntington's disease and therapeutic efficacy of antagonist administration. FASEB J 23(6):1893-906. [PubMed: 19171786]  [MGI Ref ID J:150552]

Eckermann K; Mocanu MM; Khlistunova I; Biernat J; Nissen A; Hofmann A; Schonig K; Bujard H; Haemisch A; Mandelkow E; Zhou L; Rune G; Mandelkow EM. 2007. The beta-propensity of Tau determines aggregation and synaptic loss in inducible mouse models of tauopathy. J Biol Chem 282(43):31755-65. [PubMed: 17716969]  [MGI Ref ID J:126788]

Engel T; Goni-Oliver P; Gomez-Ramos P; Moran MA; Lucas JJ; Avila J; Hernandez F. 2008. Hippocampal neuronal subpopulations are differentially affected in double transgenic mice overexpressing frontotemporal dementia and parkinsonism linked to chromosome 17 tau and glycogen synthase kinase-3beta. Neuroscience 157(4):772-80. [PubMed: 18951953]  [MGI Ref ID J:144874]

Engel T; Lucas JJ; Gomez-Ramos P; Moran MA; Avila J; Hernandez F. 2006. Cooexpression of FTDP-17 tau and GSK-3beta in transgenic mice induce tau polymerization and neurodegeneration. Neurobiol Aging 27(9):1258-68. [PubMed: 16054268]  [MGI Ref ID J:113439]

Favilla C; Abel T; Kelly MP. 2008. Chronic Galphas signaling in the striatum increases anxiety-related behaviors independent of developmental effects. J Neurosci 28(51):13952-6. [PubMed: 19091983]  [MGI Ref ID J:143516]

Fridmacher V; Kaltschmidt B; Goudeau B; Ndiaye D; Rossi FM; Pfeiffer J; Kaltschmidt C; Israel A; Memet S. 2003. Forebrain-specific neuronal inhibition of nuclear factor-kappaB activity leads to loss of neuroprotection. J Neurosci 23(28):9403-8. [PubMed: 14561868]  [MGI Ref ID J:86217]

Fukui H; Moraes CT. 2009. Mechanisms of formation and accumulation of mitochondrial DNA deletions in aging neurons. Hum Mol Genet 18(6):1028-36. [PubMed: 19095717]  [MGI Ref ID J:145737]

Gomez-Sintes R; Hernandez F; Bortolozzi A; Artigas F; Avila J; Zaratin P; Gotteland JP; Lucas JJ. 2007. Neuronal apoptosis and reversible motor deficit in dominant-negative GSK-3 conditional transgenic mice. EMBO J 26(11):2743-54. [PubMed: 17510631]  [MGI Ref ID J:122627]

Gross C; Zhuang X; Stark K; Ramboz S; Oosting R; Kirby L; Santarelli L; Beck S; Hen R. 2002. Serotonin1A receptor acts during development to establish normal anxiety-like behaviour in the adult. Nature 416(6879):396-400. [PubMed: 11919622]  [MGI Ref ID J:75722]

Hasan MT; Schonig K; Berger S; Graewe W; Bujard H. 2001. Long-term, noninvasive imaging of regulated gene expression in living mice. Genesis 29(3):116-22. [PubMed: 11252052]  [MGI Ref ID J:127660]

Hidvegi T; Schmidt BZ; Hale P; Perlmutter DH. 2005. Accumulation of mutant alpha1-antitrypsin Z in the endoplasmic reticulum activates caspases-4 and -12, NFkappaB, and BAP31 but not the unfolded protein response. J Biol Chem 280(47):39002-15. [PubMed: 16183649]  [MGI Ref ID J:104112]

Isiegas C; McDonough C; Huang T; Havekes R; Fabian S; Wu LJ; Xu H; Zhao MG; Kim JI; Lee YS; Lee HR; Ko HG; Lee N; Choi SL; Lee JS; Son H; Zhuo M; Kaang BK; Abel T. 2008. A novel conditional genetic system reveals that increasing neuronal cAMP enhances memory and retrieval. J Neurosci 28(24):6220-30. [PubMed: 18550764]  [MGI Ref ID J:137375]

Jankowsky JL; Slunt HH; Gonzales V; Savonenko AV; Wen JC; Jenkins NA; Copeland NG; Younkin LH; Lester HA; Younkin SG; Borchelt DR. 2005. Persistent amyloidosis following suppression of Abeta production in a transgenic model of Alzheimer disease. PLoS Med 2(12):e355. [PubMed: 16279840]  [MGI Ref ID J:109829]

Jerecic J; Schulze CH; Jonas P; Sprengel R; Seeburg PH; Bischofberger J. 2001. Impaired NMDA receptor function in mouse olfactory bulb neurons by tetracycline-sensitive NR1 (N598R) expression. Brain Res Mol Brain Res 94(1-2):96-104. [PubMed: 11597769]  [MGI Ref ID J:130561]

Kaltschmidt B; Ndiaye D; Korte M; Pothion S; Arbibe L; Prullage M; Pfeiffer J; Lindecke A; Staiger V; Israel A; Kaltschmidt C; Memet S. 2006. NF-kappaB regulates spatial memory formation and synaptic plasticity through protein kinase A/CREB signaling. Mol Cell Biol 26(8):2936-46. [PubMed: 16581769]  [MGI Ref ID J:107412]

Krestel HE; Mihaljevic AL; Hoffman DA; Schneider A. 2004. Neuronal co-expression of EGFP and beta-galactosidase in mice causes neuropathology and premature death. Neurobiol Dis 17(2):310-8. [PubMed: 15474368]  [MGI Ref ID J:93090]

Krestel HE; Shimshek DR; Jensen V; Nevian T; Kim J; Geng Y; Bast T; Depaulis A; Schonig K; Schwenk F; Bujard H; Hvalby O; Sprengel R; Seeburg PH. 2004. A genetic switch for epilepsy in adult mice. J Neurosci 24(46):10568-78. [PubMed: 15548671]  [MGI Ref ID J:96556]

Lee AS; Duman RS; Pittenger C. 2008. A double dissociation revealing bidirectional competition between striatum and hippocampus during learning. Proc Natl Acad Sci U S A 105(44):17163-8. [PubMed: 18955704]  [MGI Ref ID J:144070]

Lee B; Cao R; Choi YS; Cho HY; Rhee AD; Hah CK; Hoyt KR; Obrietan K. 2009. The CREB/CRE transcriptional pathway: protection against oxidative stress-mediated neuronal cell death. J Neurochem 108(5):1251-65. [PubMed: 19141071]  [MGI Ref ID J:146168]

Lee HG; Casadesus G; Nunomura A; Zhu X; Castellani RJ; Richardson SL; Perry G; Felsher DW; Petersen RB; Smith MA. 2009. The neuronal expression of MYC causes a neurodegenerative phenotype in a novel transgenic mouse. Am J Pathol 174(3):891-7. [PubMed: 19164506]  [MGI Ref ID J:146151]

Lindeberg J; Mattsson R; Ebendal T. 2002. Timing the doxycycline yields different patterns of genomic recombination in brain neurons with a new inducible Cre transgene. J Neurosci Res 68(2):248-53. [PubMed: 11948670]  [MGI Ref ID J:82535]

Lopez de Armentia M; Jancic D; Olivares R; Alarcon JM; Kandel ER; Barco A. 2007. cAMP response element-binding protein-mediated gene expression increases the intrinsic excitability of CA1 pyramidal neurons. J Neurosci 27(50):13909-18. [PubMed: 18077703]  [MGI Ref ID J:130579]

Lucas JJ; Hernandez F; Gomez-Ramos P; Moran MA; Hen R; Avila J. 2001. Decreased nuclear beta-catenin, tau hyperphosphorylation and neurodegeneration in GSK-3beta conditional transgenic mice. EMBO J 20(1-2):27-39. [PubMed: 11226152]  [MGI Ref ID J:67070]

Mack V; Burnashev N; Kaiser KM; Rozov A; Jensen V; Hvalby O; Seeburg PH; Sakmann B; Sprengel R. 2001. Conditional restoration of hippocampal synaptic potentiation in Glur-A-deficient mice. Science 292(5526):2501-4. [PubMed: 11431570]  [MGI Ref ID J:76021]

Marxreiter F; Nuber S; Kandasamy M; Klucken J; Aigner R; Burgmayer R; Couillard-Despres S; Riess O; Winkler J; Winner B. 2009. Changes in adult olfactory bulb neurogenesis in mice expressing the A30P mutant form of alpha-synuclein. Eur J Neurosci 29(5):879-90. [PubMed: 19291219]  [MGI Ref ID J:147049]

McKinney BC; Schneider JS; Schafer GL; Lowing JL; Mohan S; Zhao MX; Heng MY; Albin RL; Seasholtz AF; Akil H; Murphy GG. 2008. Decreased locomotor activity in mice expressing tTA under control of the CaMKII alpha promoter. Genes Brain Behav 7(2):203-13. [PubMed: 17640289]  [MGI Ref ID J:145601]

Morozov A; Muzzio IA; Bourtchouladze R; Van-Strien N; Lapidus K; Yin D; Winder DG; Adams JP; Sweatt JD; Kandel ER. 2003. Rap1 couples cAMP signaling to a distinct pool of p42/44MAPK regulating excitability, synaptic plasticity, learning, and memory. Neuron 39(2):309-25. [PubMed: 12873387]  [MGI Ref ID J:128795]

Muyllaert D; Terwel D; Kremer A; Sennvik K; Borghgraef P; Devijver H; Dewachter I; Van Leuven F. 2008. Neurodegeneration and neuroinflammation in cdk5/p25-inducible mice: a model for hippocampal sclerosis and neocortical degeneration. Am J Pathol 172(2):470-85. [PubMed: 18202185]  [MGI Ref ID J:131373]

Nakagawa S; Kim JE; Lee R; Malberg JE; Chen J; Steffen C; Zhang YJ; Nestler EJ; Duman RS. 2002. Regulation of neurogenesis in adult mouse hippocampus by cAMP and the cAMP response element-binding protein. J Neurosci 22(9):3673-82. [PubMed: 11978843]  [MGI Ref ID J:128620]

Nakashiba T; Young JZ; McHugh TJ; Buhl DL; Tonegawa S. 2008. Transgenic inhibition of synaptic transmission reveals role of CA3 output in hippocampal learning. Science 319(5867):1260-4. [PubMed: 18218862]  [MGI Ref ID J:131852]

Nicholls RE; Alarcon JM; Malleret G; Carroll RC; Grody M; Vronskaya S; Kandel ER. 2008. Transgenic mice lacking NMDAR-dependent LTD exhibit deficits in behavioral flexibility. Neuron 58(1):104-17. [PubMed: 18400167]  [MGI Ref ID J:135145]

Nicholls RE; Zhang XL; Bailey CP; Conklin BR; Kandel ER; Stanton PK. 2006. mGluR2 acts through inhibitory Galpha subunits to regulate transmission and long-term plasticity at hippocampal mossy fiber-CA3 synapses. Proc Natl Acad Sci U S A 103(16):6380-5. [PubMed: 16606834]  [MGI Ref ID J:109030]

Nie T; McDonough CB; Huang T; Nguyen PV; Abel T. 2007. Genetic disruption of protein kinase A anchoring reveals a role for compartmentalized kinase signaling in theta-burst long-term potentiation and spatial memory. J Neurosci 27(38):10278-88. [PubMed: 17881534]  [MGI Ref ID J:145104]

Niewoehner B; Single FN; Hvalby O; Jensen V; Borgloh SM; Seeburg PH; Rawlins JN; Sprengel R; Bannerman DM. 2007. Impaired spatial working memory but spared spatial reference memory following functional loss of NMDA receptors in the dentate gyrus. Eur J Neurosci 25(3):837-46. [PubMed: 17313573]  [MGI Ref ID J:119834]

Nuber S; Petrasch-Parwez E; Winner B; Winkler J; von Horsten S; Schmidt T; Boy J; Kuhn M; Nguyen HP; Teismann P; Schulz JB; Neumann M; Pichler BJ; Reischl G; Holzmann C; Schmitt I; Bornemann A; Kuhn W; Zimmermann F; Servadio A; Riess O. 2008. Neurodegeneration and motor dysfunction in a conditional model of Parkinson's disease. J Neurosci 28(10):2471-84. [PubMed: 18322092]  [MGI Ref ID J:132774]

Pletnikov MV; Ayhan Y; Nikolskaia O; Xu Y; Ovanesov MV; Huang H; Mori S; Moran TH; Ross CA. 2008. Inducible expression of mutant human DISC1 in mice is associated with brain and behavioral abnormalities reminiscent of schizophrenia. Mol Psychiatry 13(2):173-86, 115. [PubMed: 17848917]  [MGI Ref ID J:146881]

Ramsden M; Kotilinek L; Forster C; Paulson J; McGowan E; SantaCruz K; Guimaraes A; Yue M; Lewis J; Carlson G; Hutton M; Ashe KH. 2005. Age-dependent neurofibrillary tangle formation, neuron loss, and memory impairment in a mouse model of human tauopathy (P301L). J Neurosci 25(46):10637-47. [PubMed: 16291936]  [MGI Ref ID J:102973]

Sakai N; Tsubokawa H; Matsuzaki M; Kajimoto T; Takahashi E; Ren Y; Ohmori S; Shirai Y; Matsubayashi H; Chen J; Duman RS; Kasai H; Saito N. 2004. Propagation of gammaPKC translocation along the dendrites of Purkinje cell in gammaPKC-GFP transgenic mice. Genes Cells 9(10):945-57. [PubMed: 15461665]  [MGI Ref ID J:133986]

Sano Y; Ornthanalai VG; Yamada K; Homma C; Suzuki H; Suzuki T; Murphy NP; Itohara S. 2009. X11-like protein deficiency is associated with impaired conflict resolution in mice. J Neurosci 29(18):5884-96. [PubMed: 19420255]  [MGI Ref ID J:148481]

Svenningsson P; Chergui K; Rachleff I; Flajolet M; Zhang X; Yacoubi ME; Vaugeois JM; Nomikos GG; Greengard P. 2006. Alterations in 5-HT1B receptor function by p11 in depression-like states. Science 311(5757):77-80. [PubMed: 16400147]  [MGI Ref ID J:104116]

Wang L; Xie C; Greggio E; Parisiadou L; Shim H; Sun L; Chandran J; Lin X; Lai C; Yang WJ; Moore DJ; Dawson TM; Dawson VL; Chiosis G; Cookson MR; Cai H. 2008. The chaperone activity of heat shock protein 90 is critical for maintaining the stability of leucine-rich repeat kinase 2. J Neurosci 28(13):3384-91. [PubMed: 18367605]  [MGI Ref ID J:133490]

Ward NL; Putoczki T; Mearow K; Ivanco TL; Dumont DJ. 2005. Vascular-specific growth factor angiopoietin 1 is involved in the organization of neuronal processes. J Comp Neurol 482(3):244-56. [PubMed: 15690488]  [MGI Ref ID J:95553]

Wiltgen BJ; Law M; Ostlund S; Mayford M; Balleine BW. 2007. The influence of Pavlovian cues on instrumental performance is mediated by CaMKII activity in the striatum. Eur J Neurosci 25(8):2491-7. [PubMed: 17445244]  [MGI Ref ID J:125036]

Yamamoto A; Lucas JJ; Hen R. 2000. Reversal of neuropathology and motor dysfunction in a conditional model of Huntington's disease [see comments] Cell 101(1):57-66. [PubMed: 10778856]  [MGI Ref ID J:61490]

Yamasaki TR; Blurton-Jones M; Morrissette DA; Kitazawa M; Oddo S; LaFerla FM. 2007. Neural stem cells improve memory in an inducible mouse model of neuronal loss. J Neurosci 27(44):11925-33. [PubMed: 17978032]  [MGI Ref ID J:127469]

Yasuda M; Mayford MR. 2006. CaMKII activation in the entorhinal cortex disrupts previously encoded spatial memory. Neuron 50(2):309-18. [PubMed: 16630840]  [MGI Ref ID J:108340]

Zeng H; Horie K; Madisen L; Pavlova MN; Gragerova G; Rohde AD; Schimpf BA; Liang Y; Ojala E; Kramer F; Roth P; Slobodskaya O; Dolka I; Southon EA; Tessarollo L; Bornfeldt KE; Gragerov A; Pavlakis GN; Gaitanaris GA. 2008. An inducible and reversible mouse genetic rescue system. PLoS Genet 4(5):e1000069. [PubMed: 18464897]  [MGI Ref ID J:136987]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygous mice can be bred together or to wildtype siblings or C57BL/6J inbred mice.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   14-APR-08
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice (US dollars $)GenderGenotypes Provided
Individual Mouse $243.50Female or MaleHemizygous for Tg(Camk2a-tTA)1Mmay
Pairs /Price (US dollars $)Pair Genotype
$297.85Hemizygous for Tg(Camk2a-tTA)1Mmay x Noncarrier
$297.85Noncarrier x Hemizygous for Tg(Camk2a-tTA)1Mmay

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice (US dollars $)GenderGenotypes Provided
Individual Mouse $316.60Female or MaleHemizygous for Tg(Camk2a-tTA)1Mmay
Pairs /Price (US dollars $)Pair Genotype
$387.30Hemizygous for Tg(Camk2a-tTA)1Mmay x Noncarrier
$387.30Noncarrier x Hemizygous for Tg(Camk2a-tTA)1Mmay

Additional Supply Details

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

      Purchasing Information
      JAX® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.

Contact information

General inquiries

Contracts Administration

phone:207-288-6470
fax:207-288-6655

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(3.12)