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Strain Name:

B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ

Stock Number:

007004

Availability:

Repository- Live

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General Terms and Conditions
Additional Licensing and Use Restrictions

Former Name      B6.CBA-Tg(Camk2a-tTA)1Mmay/DboJ    (Changed: 28-AUG-07 )
Genes & Alleles   Camk2a;   tTA;   Tg(Camk2a-tTA)1Mmay;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Mating SystemNoncarrier x Hemizygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator David Borchelt,   McKnight Brain Inst, Univ of Florida
GenerationN9+N1 (20-DEC-07)

Strain Description
Transgenic mice expressing the tetracycline-controlled transactivator protein (tTA) under regulatory control of the forebrain-specific calcium-calmodulin-dependent kinase II (Camk2a) promoter are viable and fertile. When hemizygotes are mated to a second strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene can be blocked by administration of the tetracycline analog, doxycycline (dox). These mice are a "Tet-Off" tool that allow the inducible expression of genes in forebrain neurons, and may be useful in studying brain disorders such as Alzheimer's disease (when used in conjunction with Stock No. 005706, Stock No. 007049, Stock No. 007051, Stock No. 007052), Parkinson's disease, or other neurodegenerative diseases.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Strain Development
A transgenic construct was designed with 8.5 kb of the mouse CaMKIIalpha promoter placed upstream of the tetracycline-regulated transactivator (tTA or "Tet-Off") gene (flanked by an artificial intron and splice sites at the 5' end and by a polyadenylation signal from SV40 at the 3' end). Founder line B was established and maintained on a mixed B6;CBA genetic background upon its arrival at The Jackson Laboratory (as Stock No. 003010). These mice were obtained by Dr. David Borchelt (University of Florida) and then backcrossed to C57BL/6J inbred mice for at least 8 generations prior to sending back to The Jackson Laboratory (as Stock No. 007004).

Gene & Allele Details

Allele Symbol Tg(Camk2a-tTA)1Mmay
Allele Name transgene insertion 1, Mark Mayford
Common Name(s) CAMK-rTA; CaMKIIalpha-tTA; CamDAT; Tg(alphacre); Tg(CaMKIItTA)Mmay; Tgalpha-CaMkII-tTA; pCaMKII-tTA;
Mutation Made By Mark Mayford,   The Scripps Research Institute
Site of ExpressionExpresses tTA in forebrain neurons.
Expressed Gene tTA, tetracycline-controlled transactivator, E. coli
The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter.
Promoter Camk2a, calcium/calmodulin-dependent protein kinase II alpha, mouse, laboratory
General Note This transgene is line B.

Transgenic mice are viable, fertile, and display no overt phenotypic defects. Administration of tetracycline analogs such as doxycycline blocks transgene expression.

Molecular Note The transgene contains the tetracycline-controlled transactivator protein (tTA) under regulatory control of the forebrain specific calcium/calmodulin-dependent kinase II promoter. [MGI Ref ID J:37107]

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(tTA)
Tg(tTA) QPCR

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygous mice can be bred together or to wildtype siblings or C57BL/6J inbred mice.
Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying   Tg(Camk2a-tTA)1Mmay allele
003010   B6;CBA-Tg(Camk2a-tTA)1Mmay/J
View Strains carrying   Tg(Camk2a-tTA)1Mmay     (1 strain)

View Strains carrying other alleles of Camk2a     (9 strains)

View Strains carrying other alleles of tTA     (18 strains)

Additional Web Information

Congenic Nomenclature
Tet Expression Systems
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Alzheimer's Disease
Behavioral and Learning Defects
Parkinson's Disease
Tet Expression System (tTA/rtTA Expressing Strains)

Research Tools
Neurobiology Research (Tetop Tet System)
Tet Expression Systems (tTA/rtTA Expressing Strains)

References

Selected Reference(s)

Mayford M; Bach ME; Huang YY; Wang L; Hawkins RD; Kandel ER. 1996. Control of memory formation through regulated expression of a CaMKII transgene. Science 274(5293):1678-83. [PubMed: 8939850]  [MGI Ref ID J:37107]

Additional References

Price and Supply Information

Strain Name: B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ
Stock Number: 007004

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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