Strain Name:

B6Ei.129-Nr0b1tm1Lja/EiJ

Stock Number:

007006

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Availability:

Cryopreserved - Ready for recovery

Common Names: 6-Dax1flox;    

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN10F9pN1
Generation Definitions
 
Donating Investigator Eva Eicher,   The Jackson Laboratory

Development
The Nr0b1tm1Lja floxed targeted mutation was generated in the mixed 129-derived ES cell line R1 and the resulting mice were bred with 129S1/SvImJ. This floxed allele arrived at The Jackson Laboratory on a primarily 129 background and was backcrossed once to C57BL/6J and then backcrossed repeatedly to C57BL/6JEi in the laboratory of Dr. Eva Eicher.

Related Strains

Strains carrying other alleles of Nr0b1
006305   B6Ei.Cg-Nr0b1tm1.1Lja Tg(Sry)2Ei Chr YAKR/EiJ
007007   D2.129(B6)-Nr0b1tm1.1Lja/EiJ
View Strains carrying other alleles of Nr0b1     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
46,xy Sex Reversal 2; SRXY2   (NR0B1)
Adrenal Hypoplasia, Congenital; AHC   (NR0B1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Nr0b1tm1Lja/Y

        C57BL/6JEi
  • reproductive system phenotype
  • abnormal primary sex determination   (MGI Ref ID J:98922)
    • primary sex reversal
      • complete male to female sex-reversal is observed   (MGI Ref ID J:98922)
      • QTLs from B6 Chr4, Chr1, and a genetic interaction between Chr1 and Chr8 are implicated in the sex reversal   (MGI Ref ID J:98922)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Nr0b1tm1Lja/Y

        involves: C57BL/6JEi * DBA/2J
  • reproductive system phenotype
  • abnormal primary sex determination
    • in the presence of a Mus domesticus poschiavinus Y chromosome, this background produces XY mice with the following gonad development: 13.8% have testes; 70.7% contain both ovarian and testicular tissue and 15.5% are completely sex-reversed.   (MGI Ref ID J:98922)
    • ovotestis
      • partial sex reversal was seen with 70.7% of the hemizygotes having at least one ovotestis.   (MGI Ref ID J:98922)
    • primary sex reversal
      • on this background 15.5% of the hemizygotes show male to female sex reversal.   (MGI Ref ID J:98922)

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Nr0b1tm1Lja
Allele Name targeted mutation 1, J Larry Jameson
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Ahchflox2; B6-DAX1; Dax1flox;
Mutation Made By Linda Washburn,   The Jackson Laboratory
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Nr0b1, nuclear receptor subfamily 0, group B, member 1
Chromosome X
Gene Common Name(s) AHC; AHCH; AHX; Ahc; Ahch; DAX-1; DAX1; DSS; GTD; HHG; NROB1; SRXY2; adrenal hypoplasia, congenital; adrenal hypoplasia, congenital homolog (human);
Molecular Note A floxed PGK-neomycin resistance cassette was inserted downstream of exon 2 and a third loxP site was inserted upstream of the splice acceptor signal in exon 2. [MGI Ref ID J:51292]

Genotyping

Genotyping Information

Genotyping Protocols

Nr0b1tm1Lja, tm1.1Lja, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Nr0b1tm1Lja related

Achermann JC; Meeks JJ; Larry Jameson J. 2001. Phenotypic spectrum of mutations in DAX-1 and SF-1. Mol Cell Endocrinol 185(1-2):17-25. [PubMed: 11738790]  [MGI Ref ID J:73634]

Bouma GJ; Albrecht KH; Washburn LL; Recknagel AK; Churchill GA; Eicher EM. 2005. Gonadal sex reversal in mutant Dax1 XY mice: a failure to upregulate Sox9 in pre-Sertoli cells. Development 132(13):3045-54. [PubMed: 15944188]  [MGI Ref ID J:98922]

Freedman BD; Kempna PB; Carlone DL; Shah MS; Guagliardo NA; Barrett PQ; Gomez-Sanchez CE; Majzoub JA; Breault DT. 2013. Adrenocortical zonation results from lineage conversion of differentiated zona glomerulosa cells. Dev Cell 26(6):666-73. [PubMed: 24035414]  [MGI Ref ID J:204614]

Roy A; Matzuk MM. 2006. Deconstructing mammalian reproduction: using knockouts to define fertility pathways. Reproduction 131(2):207-19. [PubMed: 16452715]  [MGI Ref ID J:108425]

Yu RN; Ito M; Saunders TL; Camper SA; Jameson JL. 1998. Role of Ahch in gonadal development and gametogenesis [see comments] Nat Genet 20(4):353-7. [PubMed: 9843206]  [MGI Ref ID J:51292]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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