Strain Name:

STOCK Krt18tm1Tmm/J

Stock Number:

007029

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C.Cg-Krt18tm1Tmm/J    (Changed: 19-APR-07 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationN1F2pN1
Generation Definitions
 
Donating Investigator M. Bishr Omary,   Stanford University & Palo Alto VA Medic

Description
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Hepatocytes are completely devoid of keratin filaments, but desmosomes are formed and maintained. At 18 months of age, mice consistently show a distinctive liver pathology with abnormal hepatocytes containing K8 (KRT8)-positive aggregates (Mallory bodies). No KRT18 protein is produced from the targeted gene, as determined by Western blot of liver tissue. This mutant mouse strain may be useful in studies pertaining to keratin 18 (Krt18) and its partner keratin 8 (Krt8) function in glandular tissues such as liver and pancreas.

Development
A targeting vector was designed to replace exons 1 and part of 2 of the gene with an HPRT minigene driven by a mouse phosphoglycerate-kinase (PGK) promoter. Linearized vector was electroporated into 129P2/OlaHsd-derived HM-1 embryonic stem (ES) cells. Targeted clones were introduced to C57BL/6 or BALB/c blastocysts. The background of this strain is a mix of MF1, 129 and FVB. Upon arrival at The Jackson Laboratory, mutant mice were crossed to FVB/NJ inbred mice for at least one generation.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Krt18tm1Tmm/Krt18tm1Tmm

        involves: 129/Sv * 129P2/OlaHsd * MF1
  • liver/biliary system phenotype
  • enlarged liver
    • hepatomegaly by 18 months of age   (MGI Ref ID J:46610)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Internal/Organ Research
Liver Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Krt18tm1Tmm
Allele Name targeted mutation 1, Thomas M Magin
Allele Type Targeted (knock-out)
Common Name(s) K18 null; K18-;
Mutation Made By Guozhong Tao,   Stanford University
Strain of Origin129P2/OlaHsd-Hprt
ES Cell Line NameHM-1
ES Cell Line Strain129P2/OlaHsd-Hprt
Gene Symbol and Name Krt18, keratin 18
Chromosome 15
Gene Common Name(s) CK18; CYK18; Endo B; K18; Krt1-18; keratin complex 1, acidic, gene 18;
Molecular Note An HPRT minigene replaced most of exon 1, intron 1 and exon 2. [MGI Ref ID J:46610]

Genotyping

Genotyping Information

Genotyping Protocols

Krt18tm1Tmm, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Magin TM; Schroder R; Leitgeb S; Wanninger F; Zatloukal K; Grund C; Melton DW. 1998. Lessons from keratin 18 knockout mice: formation of novel keratin filaments, secondary loss of keratin 7 and accumulation of liver-specific keratin 8-positive aggregates. J Cell Biol 140(6):1441-51. [PubMed: 9508776]  [MGI Ref ID J:46610]

Additional References

Krt18tm1Tmm related

Caulin C; Ware CF; Magin TM; Oshima RG. 2000. Keratin-dependent, epithelial resistance to tumor necrosis factor-induced apoptosis. J Cell Biol 149(1):17-22. [PubMed: 10747083]  [MGI Ref ID J:121814]

Haybaeck J; Stumptner C; Thueringer A; Kolbe T; Magin TM; Hesse M; Fickert P; Tsybrovskyy O; Muller H; Trauner M; Zatloukal K; Denk H. 2012. Genetic background effects of keratin 8 and 18 in a DDC-induced hepatotoxicity and Mallory-Denk body formation mouse model. Lab Invest 92(6):857-67. [PubMed: 22449798]  [MGI Ref ID J:184713]

Hesse M; Franz T; Tamai Y; Taketo MM; Magin TM. 2000. Targeted deletion of keratins 18 and 19 leads to trophoblast fragility and early embryonic lethality EMBO J 19(19):5060-70. [PubMed: 11013209]  [MGI Ref ID J:65090]

Ku NO; Omary MB. 2006. A disease- and phosphorylation-related nonmechanical function for keratin 8. J Cell Biol 174(1):115-25. [PubMed: 16818723]  [MGI Ref ID J:111195]

Lee J; Jang KH; Kim H; Lim Y; Kim S; Yoon HN; Chung IK; Roth J; Ku NO. 2013. Predisposition to apoptosis in keratin 8-null liver is related to inactivation of NF-kappaB and SAPKs but not decreased c-Flip. Biol Open 2(7):695-702. [PubMed: 23862017]  [MGI Ref ID J:199436]

Leifeld L; Kothe S; Sohl G; Hesse M; Sauerbruch T; Magin TM; Spengler U. 2009. Keratin 18 provides resistance to Fas-mediated liver failure in mice. Eur J Clin Invest 39(6):481-8. [PubMed: 19397691]  [MGI Ref ID J:165765]

Tao GZ; Toivola DM; Zhong B; Michie SA; Resurreccion EZ; Tamai Y; Taketo MM; Omary MB. 2003. Keratin-8 null mice have different gallbladder and liver susceptibility to lithogenic diet-induced injury. J Cell Sci 116(Pt 22):4629-38. [PubMed: 14576356]  [MGI Ref ID J:86719]

Toivola DM; Nieminen MI; Hesse M; He T; Baribault H; Magin TM; Omary MB; Eriksson JE. 2001. Disturbances in hepatic cell-cycle regulation in mice with assembly-deficient keratins 8/18. Hepatology 34(6):1174-83. [PubMed: 11732007]  [MGI Ref ID J:106541]

Watson ED; Geary-Joo C; Hughes M; Cross JC. 2007. The Mrj co-chaperone mediates keratin turnover and prevents the formation of toxic inclusion bodies in trophoblast cells of the placenta. Development 134(9):1809-17. [PubMed: 17409114]  [MGI Ref ID J:121264]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, heterozygotes or homozygotes may be used for breeding.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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