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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names C.Cg-Krt18tm1Tmm/J (Changed: 19-APR-07 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation N1F0 (01-AUG-07) Donating Investigator M. Bishr Omary, Stanford University & Palo Alto VA Medic Description
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Hepatocytes are completely devoid of keratin filaments, but desmosomes are formed and maintained. At 18 months of age, mice consistently show a distinctive liver pathology with abnormal hepatocytes containing K8 (KRT8)-positive aggregates (Mallory bodies). No KRT18 protein is produced from the targeted gene, as determined by Western blot of liver tissue. This mutant mouse strain may be useful in studies pertaining to keratin 18 (Krt18) and its partner keratin 8 (Krt8) function in glandular tissues such as liver and pancreas.Development
A targeting vector was designed to replace exons 1 and part of 2 of the gene with an HPRT minigene driven by a mouse phosphoglycerate-kinase (PGK) promoter. Linearized vector was electroporated into 129P2/OlaHsd-derived HM-1 embryonic stem (ES) cells. Targeted clones were introduced to C57BL/6 or BALB/c blastocysts. The background of this strain is a mix of MF1, 129 and FVB. Upon arrival at The Jackson Laboratory, mutant mice were crossed to FVB/NJ inbred mice for at least one generation.
Control Information
| Control | ||
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| None Available | ||
| Considerations for Choosing Controls | ||
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Krt18tm1Tmm/Krt18tm1Tmm
involves: 129/Sv * 129P2/OlaHsd * MF1
- liver/biliary system phenotype
- enlarged liver (MGI Ref ID J:46610)
- hepatomegaly by 18 months of age
This mouse can be used to support research in many areas including:
Internal/Organ Research
Liver Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Krt18tm1Tmm | ||
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| Allele Name | targeted mutation 1, Thomas M Magin | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | K18 null; K18-; | ||
| Mutation Made By | Guozhong Tao, Stanford University | ||
| Strain of Origin | 129P2/OlaHsd-Hprt1b-m3 | ||
| ES Cell Line Name | HM-1 | ||
| ES Cell Line Strain | 129P2/OlaHsd-Hprt1 | ||
| Gene Symbol and Name | Krt18, keratin 18 | ||
| Chromosome | 15 | ||
| Gene Common Name(s) | CK18; CYK18; Endo B; K18; Krt1-18; MGC109143; keratin complex 1, acidic, gene 18; | ||
| Molecular Note | An HPRT minigene replaced most of exon 1, intron 1 and exon 2. [MGI Ref ID J:46610] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Krt18tm1Tmm, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Magin TM; Schroder R; Leitgeb S; Wanninger F; Zatloukal K; Grund C; Melton DW. 1998. Lessons from keratin 18 knockout mice: formation of novel keratin filaments, secondary loss of keratin 7 and accumulation of liver-specific keratin 8-positive aggregates. J Cell Biol 140(6):1441-51. [PubMed: 9508776] [MGI Ref ID J:46610]
Additional References
Krt18tm1Tmm relatedCaulin C; Ware CF; Magin TM; Oshima RG. 2000. Keratin-dependent, epithelial resistance to tumor necrosis factor-induced apoptosis. J Cell Biol 149(1):17-22. [PubMed: 10747083] [MGI Ref ID J:121814]
Hesse M; Franz T; Tamai Y; Taketo MM; Magin TM. 2000. Targeted deletion of keratins 18 and 19 leads to trophoblast fragility and early embryonic lethality EMBO J 19(19):5060-70. [PubMed: 11013209] [MGI Ref ID J:65090]
Ku NO; Omary MB. 2006. A disease- and phosphorylation-related nonmechanical function for keratin 8. J Cell Biol 174(1):115-25. [PubMed: 16818723] [MGI Ref ID J:111195]
Tao GZ; Toivola DM; Zhong B; Michie SA; Resurreccion EZ; Tamai Y; Taketo MM; Omary MB. 2003. Keratin-8 null mice have different gallbladder and liver susceptibility to lithogenic diet-induced injury. J Cell Sci 116(Pt 22):4629-38. [PubMed: 14576356] [MGI Ref ID J:86719]
Toivola DM; Nieminen MI; Hesse M; He T; Baribault H; Magin TM; Omary MB; Eriksson JE. 2001. Disturbances in hepatic cell-cycle regulation in mice with assembly-deficient keratins 8/18. Hepatology 34(6):1174-83. [PubMed: 11732007] [MGI Ref ID J:106541]
Watson ED; Geary-Joo C; Hughes M; Cross JC. 2007. The Mrj co-chaperone mediates keratin turnover and prevents the formation of toxic inclusion bodies in trophoblast cells of the placenta. Development 134(9):1809-17. [PubMed: 17409114] [MGI Ref ID J:121264]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.70 Female or Male Homozygous for Krt18tm1Tmm *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $311.40 Homozygous for Krt18tm1Tmm x Homozygous for Krt18tm1Tmm
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.50 Female or Male Homozygous for Krt18tm1Tmm *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $404.90 Homozygous for Krt18tm1Tmm x Homozygous for Krt18tm1Tmm
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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