Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain C57BL/6JEiJ Donor Strain 129P2/OlaHsd via E14TG2a ES cell line Generation N12F2pN1 Generation Definitions   Donating Investigator Eva Eicher,   The Jackson Laboratory

Description
Mice homozygous for this floxed allele are viable and fertile. These mutant mice have loxP sites flanking the C-terminal coding exon. When bred to Cre-recombinase expressing mice, offspring will have a deletion of this exon in the cre expressing tissue(s). These floxed mice may be useful in studying steroidogenic factors and pituitary gonadotrope function.

For example, when crossed to a strain expressing Cre recombinase in the anterior and intermediate lobes of the pituitary gland (see Stock No. 004426), this mutant mouse strain may be useful in studies of pituitary gonadotrope function.

Development
A targeting vector was designed to place a loxP site upstream, and a loxP site and neomycin resistance cassette downstream of the C-terminal coding exon of the targted gene. The construct was electroporated into 129P2/OlaHsd-derived E14TG2a embryonic stem (ES) cells. Following blastocyst injection of correctly targeted ES cells, chimeric mice transmitted this "floxed" allele to produce mutant mice. These mutant mice have been backcrossed for at least 12 generations to C57BL/6JEiJ inbred mice (Stock No. 000924).

Control Information

	Control
	000924 C57BL/6JEiJ
Considerations for Choosing Controls

Related Strains

Strains carrying   Nr5a1^tm2Klp allele

007042

D2.129P2(B6)-Nr5a1tm2Klp/EiJ

View Strains carrying   Nr5a1^tm2Klp     (1 strain)

Strains carrying other alleles of Nr5a1

003219	D2.129P2(B6)-Nr5a1tm1Klp/EiJ
006364	FVB-Tg(Nr5a1-cre)2Lowl/J
012462	STOCK Tg(Nr5a1-cre)7Lowl/J

View Strains carrying other alleles of Nr5a1     (3 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. 46,xy Sex Reversal 3; SRXY3   (NR5A1) Nuclear Receptor Subfamily 5, Group A, Member 1; NR5A1   (NR5A1) Premature Ovarian Failure 7; POF7   (NR5A1) Spermatogenic Failure 8; SPGF8   (NR5A1)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Nr5a1^tm2Klp/Nr5a1^tm2Klp

        involves: 129P2/OlaHsd

• normal phenotype
• no abnormal phenotype detected
  • homozygotes are phenotypically normal and fertile   (MGI Ref ID J:66593)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX^® Services jaxservices@jax.org for customized breeding options.

Nr5a1^tm2Klp/Nr5a1^tm2.1Klp Tg(Cga-cre)3Sac/0

        involves: 129P2/OlaHsd * C57BL/6J * SJL   (conditional)

• endocrine/exocrine gland phenotype
• abnormal Leydig cell morphology
  • mutant Leydig cells display none of the histological features typical of steroidogenic cells   (MGI Ref ID J:66593)
• • Leydig cell hypoplasia
    • interstitial Leydig cells are severely reduced in number   (MGI Ref ID J:66593)
    • treatment with exogenous gonadotropins stimulated Leydig cell hypertrophy and induced luminal opening of the seminiferous tubules   (MGI Ref ID J:66593)
• absent corpus luteum   (MGI Ref ID J:66593)
• absent mature ovarian follicles
  • although ovarian follicles develop through the primary, secondary and antral stages, no large preovulatory follicles or corpora lutea are observed   (MGI Ref ID J:66593)
  • treatment with exogenous gonadotropins (PMSG) stimulated maturation of ovarian follicles to the preovulatory stage and induced ovulation, as indicated by the presence of corpora lutea   (MGI Ref ID J:66593)
• cryptorchism
  • mutant males have cryptorchid testes   (MGI Ref ID J:66593)
• ovary hypoplasia
  • severe   (MGI Ref ID J:66593)
• prostate gland hypoplasia
  • treatment with exogenous gonadotropins stimulated enlargement of the hypoplastic prostate glands   (MGI Ref ID J:66593)
• seminal vesicle hypoplasia
  • treatment with exogenous gonadotropins stimulated enlargement of the hypoplastic seminal vesicles   (MGI Ref ID J:66593)
• testis hypoplasia
  • severe   (MGI Ref ID J:66593)
• reproductive system phenotype
• abnormal female reproductive system morphology   (MGI Ref ID J:66593)
• • absent corpus luteum   (MGI Ref ID J:66593)
  • absent mature ovarian follicles
    • although ovarian follicles develop through the primary, secondary and antral stages, no large preovulatory follicles or corpora lutea are observed   (MGI Ref ID J:66593)
    • treatment with exogenous gonadotropins (PMSG) stimulated maturation of ovarian follicles to the preovulatory stage and induced ovulation, as indicated by the presence of corpora lutea   (MGI Ref ID J:66593)
  • ovary hypoplasia
    • severe   (MGI Ref ID J:66593)
  • uterus hypoplasia
    • treatment with exogenous gonadotropins (PMSG) stimulated a significant increase in uterine size and development of the endometrial glands   (MGI Ref ID J:66593)
• abnormal male reproductive system morphology
  • mutant males show hypoplastic external and internal genitalia   (MGI Ref ID J:66593)
• • abnormal Leydig cell morphology
    • mutant Leydig cells display none of the histological features typical of steroidogenic cells   (MGI Ref ID J:66593)
  • • Leydig cell hypoplasia
      • interstitial Leydig cells are severely reduced in number   (MGI Ref ID J:66593)
      • treatment with exogenous gonadotropins stimulated Leydig cell hypertrophy and induced luminal opening of the seminiferous tubules   (MGI Ref ID J:66593)
  • cryptorchism
    • mutant males have cryptorchid testes   (MGI Ref ID J:66593)
  • external male genitalia hypoplasia   (MGI Ref ID J:66593)
  • internal male genitalia hypoplasia   (MGI Ref ID J:66593)
  • prostate gland hypoplasia
    • treatment with exogenous gonadotropins stimulated enlargement of the hypoplastic prostate glands   (MGI Ref ID J:66593)
  • seminal vesicle hypoplasia
    • treatment with exogenous gonadotropins stimulated enlargement of the hypoplastic seminal vesicles   (MGI Ref ID J:66593)
  • testis hypoplasia
    • severe   (MGI Ref ID J:66593)
• abnormal spermatogenesis
  • mutant males fail to progress through the normal stages of spermatogenesis: occasional pachytene spermatocytes are observed, but mature spermatids are absent   (MGI Ref ID J:66593)
  • treatment with exogenous gonadotropins (PMSG) stimulated gonadal steroidogenesis, inducing maturation of spermatogonial precursors to the round spermatid stage   (MGI Ref ID J:66593)
• • abnormal spermatid morphology
    • no mature spermatids are observed   (MGI Ref ID J:66593)
• absent sexual maturation
  • both male and female mutants are viable but fail to show signs of secondary sexual maturation, even at 6 months of age   (MGI Ref ID J:66593)
  • both male and female mutants exhibit sexual infantilism of the accessory sex organs   (MGI Ref ID J:66593)
• anovulation   (MGI Ref ID J:66593)
• decreased male germ cell number
  • male germ cells are significantly reduced in number   (MGI Ref ID J:66593)
• delayed vaginal opening
  • mutant vaginas fail to open at the normal age of puberty   (MGI Ref ID J:66593)
• female infertility   (MGI Ref ID J:66593)
• male infertility   (MGI Ref ID J:66593)
• small gonad
  • mutant gonads are severely hypoplastic   (MGI Ref ID J:66593)
• • ovary hypoplasia
    • severe   (MGI Ref ID J:66593)
  • testis hypoplasia
    • severe   (MGI Ref ID J:66593)
• homeostasis/metabolism phenotype
• decreased follicle stimulating hormone level
  • although mutant pituitaries appear histologically intact, immunoreactive FSH leves are virtually undetectable   (MGI Ref ID J:66593)
  • in contrast, pituitary ACTH, TSH and prolactin levels are comparable to those in wild-type pituitaries   (MGI Ref ID J:66593)
• • decreased circulating follicle stimulating hormone level
    • serum FSH levels are significantly reduced in both male and female mutants relative to wild-type controls   (MGI Ref ID J:66593)
• decreased luteinizing hormone level
  • although mutant pituitaries appear histologically intact, immunoreactive LH leves are virtually undetectable   (MGI Ref ID J:66593)
• • decreased circulating luteinizing hormone level
    • serum LH levels are significantly reduced in male mutants relative to wild-type controls   (MGI Ref ID J:66593)

Nr5a1^tm2Klp/Nr5a1^tm2Klp Tg(Cga-cre)3Sac/0

        involves: 129P2/OlaHsd * C57BL/6J * SJL   (conditional)

• reproductive system phenotype
• abnormal sex gland morphology   (MGI Ref ID J:70412)
• • Leydig cell hypoplasia
    • at 8 weeks of age, mutant interstitial Leydig cells are severely hypoplastic   (MGI Ref ID J:70412)
    • in contrast, Sertoli cells and developing germ cells (including mature sperm) are relatively intact   (MGI Ref ID J:70412)
  • absent corpus luteum   (MGI Ref ID J:70412)
  • absent mature ovarian follicles
    • neither large preovulatory follicles nor corpora lutea are observed, similar to mice that are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
  • impaired ovarian folliculogenesis
    • mutant mice display absence of follicular maturation beyond the antral stage   (MGI Ref ID J:70412)
  • prostate gland hypoplasia
    • similar to that observed in mice that are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
  • seminal vesicle hypoplasia
    • similar to that observed in mice that are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
  • small ovary
    • although mutant ovaries are smaller than wild-type, they are significantly larger than those of mice that are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
  • • decreased ovary weight
      • at 8 weeks of age, the weight of mutant ovaries (0.06 g/kg) is significantly lower than wild-type (0.47 g/kg), but higher than that of mice which are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac (0.01 g/kg)   (MGI Ref ID J:70412)
  • small testis
    • although mutant testes are smaller than wild-type, they are significantly larger than those of mice that are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
  • • decreased testis weight
      • at 8 weeks of age, the weight of mutant testes (3.7 g/kg) is significantly lower than wild-type (7.9 g/kg), but significantly higher than that of mice which are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac (0.4 g/kg)   (MGI Ref ID J:70412)
• absent sexual maturation
  • both male and female mutants show little evidence for secondary sexual maturation   (MGI Ref ID J:70412)
• female infertility   (MGI Ref ID J:70412)
• male infertility   (MGI Ref ID J:70412)
• small gonad
  • both male and female mutants show a milder degree of (hypomorphic) hypogonadism than mice that are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
• • small ovary
    • although mutant ovaries are smaller than wild-type, they are significantly larger than those of mice that are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
  • • decreased ovary weight
      • at 8 weeks of age, the weight of mutant ovaries (0.06 g/kg) is significantly lower than wild-type (0.47 g/kg), but higher than that of mice which are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac (0.01 g/kg)   (MGI Ref ID J:70412)
  • small testis
    • although mutant testes are smaller than wild-type, they are significantly larger than those of mice that are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
  • • decreased testis weight
      • at 8 weeks of age, the weight of mutant testes (3.7 g/kg) is significantly lower than wild-type (7.9 g/kg), but significantly higher than that of mice which are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac (0.4 g/kg)   (MGI Ref ID J:70412)
• uterus hypoplasia
  • similar to that observed in mice that are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
• endocrine/exocrine gland phenotype
• abnormal sex gland morphology   (MGI Ref ID J:70412)
• • Leydig cell hypoplasia
    • at 8 weeks of age, mutant interstitial Leydig cells are severely hypoplastic   (MGI Ref ID J:70412)
    • in contrast, Sertoli cells and developing germ cells (including mature sperm) are relatively intact   (MGI Ref ID J:70412)
  • absent corpus luteum   (MGI Ref ID J:70412)
  • absent mature ovarian follicles
    • neither large preovulatory follicles nor corpora lutea are observed, similar to mice that are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
  • impaired ovarian folliculogenesis
    • mutant mice display absence of follicular maturation beyond the antral stage   (MGI Ref ID J:70412)
  • prostate gland hypoplasia
    • similar to that observed in mice that are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
  • seminal vesicle hypoplasia
    • similar to that observed in mice that are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
  • small ovary
    • although mutant ovaries are smaller than wild-type, they are significantly larger than those of mice that are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
  • • decreased ovary weight
      • at 8 weeks of age, the weight of mutant ovaries (0.06 g/kg) is significantly lower than wild-type (0.47 g/kg), but higher than that of mice which are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac (0.01 g/kg)   (MGI Ref ID J:70412)
  • small testis
    • although mutant testes are smaller than wild-type, they are significantly larger than those of mice that are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
  • • decreased testis weight
      • at 8 weeks of age, the weight of mutant testes (3.7 g/kg) is significantly lower than wild-type (7.9 g/kg), but significantly higher than that of mice which are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac (0.4 g/kg)   (MGI Ref ID J:70412)
• homeostasis/metabolism phenotype
• decreased circulating follicle stimulating hormone level
  • mutant mice of both sexes show circulating FSH levels (4.5 ng/ml males, 5.2 ng/ml females) that are intermediate between those of wild-type mice (31.35 ng/ml males, 12.4 ng/ml females) and mice that are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac (1.2 ng/ml males, 2.7 ng/ml females)   (MGI Ref ID J:70412)
• decreased circulating luteinizing hormone level
  • mutant males display a severe reduction in circulating LH levels similar to that observed in mice that are heterozygous for Nr5a1^tm2Klp and Nr5a1^tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
• behavior/neurological phenotype
• abnormal sexual interaction
  • mutants do not engage in sexual activity   (MGI Ref ID J:70412)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Reproductive Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Nr5a1tm2Klp
Allele Name		targeted mutation 2, Keith L Parker
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		F; Sf1Fl;
Mutation Made By		Linda Washburn,   The Jackson Laboratory
Strain of Origin		129P2/OlaHsd
ES Cell Line Name		E14TG2a
ES Cell Line Strain		129P2/OlaHsd
Gene Symbol and Name		Nr5a1, nuclear receptor subfamily 5, group A, member 1
Chromosome		2
Gene Common Name(s)		AD4BP; Ad4BP/SF-1; ELP; FTZ1; FTZF1; Ftz-F1; Ftzf1; POF7; SF-1; SF1; SPGF8; SRXY3; adrenal 4-binding protein; fushi tarazu 1 factor homolog, (Drosophila); steroidogenic factor 1;
General Note		Phenotypic Similarity to Human Syndrome: Hypogonadotropic Hypogonadism in mice heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac (J:66593)
Molecular Note		A single loxP site was inserted into an intron 5' to the last coding exon, and a loxP site followed by a neomycin resistance cassette was inserted 3' to this exon. This mutation flanks sequences encoding motifs essential for transcriptional activity as well as the transcription termination and polyadenyulation sequences. [MGI Ref ID J:66593]

Genotyping

Genotyping Information

Genotyping Protocols

Nr5a1^tm2Klp, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Luo X; Ikeda Y; Parker KL. 1994. A cell-specific nuclear receptor is essential for adrenal and gonadal development and sexual differentiation. Cell 77(4):481-90. [PubMed: 8187173]  [MGI Ref ID J:35576]

Additional References

Nr5a1^tm2Klp related

Bakke M; Zhao L; Parker KL. 2001. Approaches to define the role of SF-1 at different levels of the hypothalamic-pituitary-steroidogenic organ axis. Mol Cell Endocrinol 179(1-2):33-7. [PubMed: 11420128]  [MGI Ref ID J:125681]

Buaas FW; Gardiner JR; Clayton S; Val P; Swain A. 2012. In vivo evidence for the crucial role of SF1 in steroid-producing cells of the testis, ovary and adrenal gland. Development 139(24):4561-70. [PubMed: 23136395]  [MGI Ref ID J:189958]

Jeyasuria P; Ikeda Y; Jamin SP; Zhao L; De Rooij DG; Themmen AP; Behringer RR; Parker KL. 2004. Cell-specific knockout of steroidogenic factor 1 reveals its essential roles in gonadal function. Mol Endocrinol 18(7):1610-9. [PubMed: 15118069]  [MGI Ref ID J:91352]

Kato T; Esaki M; Matsuzawa A; Ikeda Y. 2012. NR5A1 is required for functional maturation of Sertoli cells during postnatal development. Reproduction 143(5):663-72. [PubMed: 22419830]  [MGI Ref ID J:185215]

Kim KW; Donato J Jr; Berglund ED; Choi YH; Kohno D; Elias CF; Depinho RA; Elmquist JK. 2012. FOXO1 in the ventromedial hypothalamus regulates energy balance. J Clin Invest 122(7):2578-89. [PubMed: 22653058]  [MGI Ref ID J:190474]

Kim KW; Jo YH; Zhao L; Stallings NR; Chua SC Jr; Parker KL. 2008. Steroidogenic factor 1 regulates expression of the cannabinoid receptor 1 in the ventromedial hypothalamic nucleus. Mol Endocrinol 22(8):1950-61. [PubMed: 18511494]  [MGI Ref ID J:138418]

Kim KW; Zhao L; Donato J Jr; Kohno D; Xu Y; Elias CF; Lee C; Parker KL; Elmquist JK. 2011. Steroidogenic factor 1 directs programs regulating diet-induced thermogenesis and leptin action in the ventral medial hypothalamic nucleus. Proc Natl Acad Sci U S A 108(26):10673-8. [PubMed: 21636788]  [MGI Ref ID J:173306]

Pelusi C; Ikeda Y; Zubair M; Parker KL. 2008. Impaired follicle development and infertility in female mice lacking steroidogenic factor 1 in ovarian granulosa cells. Biol Reprod 79(6):1074-83. [PubMed: 18703422]  [MGI Ref ID J:145805]

Roy A; Matzuk MM. 2006. Deconstructing mammalian reproduction: using knockouts to define fertility pathways. Reproduction 131(2):207-19. [PubMed: 16452715]  [MGI Ref ID J:108425]

Segal JP; Stallings NR; Lee CE; Zhao L; Socci N; Viale A; Harris TM; Soares MB; Childs G; Elmquist JK; Parker KL; Friedman JM. 2005. Use of laser-capture microdissection for the identification of marker genes for the ventromedial hypothalamic nucleus. J Neurosci 25(16):4181-8. [PubMed: 15843621]  [MGI Ref ID J:145979]

Wilson MJ; Jeyasuria P; Parker KL; Koopman P. 2005. The transcription factors steroidogenic factor-1 and SOX9 regulate expression of Vanin-1 during mouse testis development. J Biol Chem 280(7):5917-23. [PubMed: 15590666]  [MGI Ref ID J:96928]

Zhao L; Bakke M; Hanley NA; Majdic G; Stallings NR; Jeyasuria P; Parker KL. 2004. Tissue-specific knockouts of steroidogenic factor 1. Mol Cell Endocrinol 215(1-2):89-94. [PubMed: 15026179]  [MGI Ref ID J:88733]

Zhao L; Bakke M; Krimkevich Y; Cushman LJ; Parlow AF; Camper SA; Parker KL. 2001. Hypomorphic phenotype in mice with pituitary-specific knockout of steroidogenic factor 1. Genesis 30(2):65-9. [PubMed: 11416865]  [MGI Ref ID J:70412]

Zhao L; Bakke M; Krimkevich Y; Cushman LJ; Parlow AF; Camper SA; Parker KL. 2001. Steroidogenic factor 1 (SF1) is essential for pituitary gonadotrope function. Development 128(2):147-54. [PubMed: 11124111]  [MGI Ref ID J:66593]

Zhao L; Kim KW; Ikeda Y; Anderson KK; Beck L; Chase S; Tobet SA; Parker KL. 2008. Central nervous system-specific knockout of steroidogenic factor 1 results in increased anxiety-like behavior. Mol Endocrinol 22(6):1403-15. [PubMed: 18372344]  [MGI Ref ID J:136159]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice are bred.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	000924 C57BL/6JEiJ
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.