Strain Name:

B6Ei.129P2-Nr5a1tm2Klp/EiJ

Stock Number:

007041

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6JEiJ
Donor Strain 129P2/OlaHsd via E14TG2a ES cell line
GenerationN12F2pN1
Generation Definitions
 
Donating Investigator Eva Eicher,   The Jackson Laboratory

Description
Mice homozygous for this floxed allele are viable and fertile. These mutant mice have loxP sites flanking the C-terminal coding exon. When bred to Cre-recombinase expressing mice, offspring will have a deletion of this exon in the cre expressing tissue(s). These floxed mice may be useful in studying steroidogenic factors and pituitary gonadotrope function.

For example, when crossed to a strain expressing Cre recombinase in the anterior and intermediate lobes of the pituitary gland (see Stock No. 004426), this mutant mouse strain may be useful in studies of pituitary gonadotrope function.

Development
A targeting vector was designed to place a loxP site upstream, and a loxP site and neomycin resistance cassette downstream of the C-terminal coding exon of the targted gene. The construct was electroporated into 129P2/OlaHsd-derived E14TG2a embryonic stem (ES) cells. Following blastocyst injection of correctly targeted ES cells, chimeric mice transmitted this "floxed" allele to produce mutant mice. These mutant mice have been backcrossed for at least 12 generations to C57BL/6JEiJ inbred mice (Stock No. 000924).

Control Information

  Control
   000924 C57BL/6JEiJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Nr5a1tm2Klp allele
007042   D2.129P2(B6)-Nr5a1tm2Klp/EiJ
View Strains carrying   Nr5a1tm2Klp     (1 strain)

Strains carrying other alleles of Nr5a1
003219   D2.129P2(B6)-Nr5a1tm1Klp/EiJ
006364   FVB-Tg(Nr5a1-cre)2Lowl/J
012462   STOCK Tg(Nr5a1-cre)7Lowl/J
View Strains carrying other alleles of Nr5a1     (3 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
46,xy Sex Reversal 3; SRXY3   (NR5A1)
Nuclear Receptor Subfamily 5, Group A, Member 1; NR5A1   (NR5A1)
Premature Ovarian Failure 7; POF7   (NR5A1)
Spermatogenic Failure 8; SPGF8   (NR5A1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Nr5a1tm2Klp/Nr5a1tm2Klp

        involves: 129P2/OlaHsd
  • normal phenotype
  • no abnormal phenotype detected
    • homozygotes are phenotypically normal and fertile   (MGI Ref ID J:66593)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Nr5a1tm2Klp/Nr5a1tm2.1Klp Tg(Cga-cre)3Sac/0

        involves: 129P2/OlaHsd * C57BL/6J * SJL   (conditional)
  • endocrine/exocrine gland phenotype
  • abnormal Leydig cell morphology
    • mutant Leydig cells display none of the histological features typical of steroidogenic cells   (MGI Ref ID J:66593)
    • Leydig cell hypoplasia
      • interstitial Leydig cells are severely reduced in number   (MGI Ref ID J:66593)
      • treatment with exogenous gonadotropins stimulated Leydig cell hypertrophy and induced luminal opening of the seminiferous tubules   (MGI Ref ID J:66593)
  • absent corpus luteum   (MGI Ref ID J:66593)
  • absent mature ovarian follicles
    • although ovarian follicles develop through the primary, secondary and antral stages, no large preovulatory follicles or corpora lutea are observed   (MGI Ref ID J:66593)
    • treatment with exogenous gonadotropins (PMSG) stimulated maturation of ovarian follicles to the preovulatory stage and induced ovulation, as indicated by the presence of corpora lutea   (MGI Ref ID J:66593)
  • cryptorchism
    • mutant males have cryptorchid testes   (MGI Ref ID J:66593)
  • ovary hypoplasia
  • prostate gland hypoplasia
    • treatment with exogenous gonadotropins stimulated enlargement of the hypoplastic prostate glands   (MGI Ref ID J:66593)
  • seminal vesicle hypoplasia
    • treatment with exogenous gonadotropins stimulated enlargement of the hypoplastic seminal vesicles   (MGI Ref ID J:66593)
  • testis hypoplasia
  • reproductive system phenotype
  • abnormal female reproductive system morphology   (MGI Ref ID J:66593)
    • absent corpus luteum   (MGI Ref ID J:66593)
    • absent mature ovarian follicles
      • although ovarian follicles develop through the primary, secondary and antral stages, no large preovulatory follicles or corpora lutea are observed   (MGI Ref ID J:66593)
      • treatment with exogenous gonadotropins (PMSG) stimulated maturation of ovarian follicles to the preovulatory stage and induced ovulation, as indicated by the presence of corpora lutea   (MGI Ref ID J:66593)
    • ovary hypoplasia
    • uterus hypoplasia
      • treatment with exogenous gonadotropins (PMSG) stimulated a significant increase in uterine size and development of the endometrial glands   (MGI Ref ID J:66593)
  • abnormal male reproductive system morphology
    • mutant males show hypoplastic external and internal genitalia   (MGI Ref ID J:66593)
    • abnormal Leydig cell morphology
      • mutant Leydig cells display none of the histological features typical of steroidogenic cells   (MGI Ref ID J:66593)
      • Leydig cell hypoplasia
        • interstitial Leydig cells are severely reduced in number   (MGI Ref ID J:66593)
        • treatment with exogenous gonadotropins stimulated Leydig cell hypertrophy and induced luminal opening of the seminiferous tubules   (MGI Ref ID J:66593)
    • cryptorchism
      • mutant males have cryptorchid testes   (MGI Ref ID J:66593)
    • external male genitalia hypoplasia   (MGI Ref ID J:66593)
    • internal male genitalia hypoplasia   (MGI Ref ID J:66593)
    • prostate gland hypoplasia
      • treatment with exogenous gonadotropins stimulated enlargement of the hypoplastic prostate glands   (MGI Ref ID J:66593)
    • seminal vesicle hypoplasia
      • treatment with exogenous gonadotropins stimulated enlargement of the hypoplastic seminal vesicles   (MGI Ref ID J:66593)
    • testis hypoplasia
  • abnormal spermatogenesis
    • mutant males fail to progress through the normal stages of spermatogenesis: occasional pachytene spermatocytes are observed, but mature spermatids are absent   (MGI Ref ID J:66593)
    • treatment with exogenous gonadotropins (PMSG) stimulated gonadal steroidogenesis, inducing maturation of spermatogonial precursors to the round spermatid stage   (MGI Ref ID J:66593)
    • abnormal spermatid morphology
      • no mature spermatids are observed   (MGI Ref ID J:66593)
  • absent sexual maturation
    • both male and female mutants are viable but fail to show signs of secondary sexual maturation, even at 6 months of age   (MGI Ref ID J:66593)
    • both male and female mutants exhibit sexual infantilism of the accessory sex organs   (MGI Ref ID J:66593)
  • anovulation   (MGI Ref ID J:66593)
  • decreased male germ cell number
    • male germ cells are significantly reduced in number   (MGI Ref ID J:66593)
  • delayed vaginal opening
    • mutant vaginas fail to open at the normal age of puberty   (MGI Ref ID J:66593)
  • female infertility   (MGI Ref ID J:66593)
  • male infertility   (MGI Ref ID J:66593)
  • small gonad
    • mutant gonads are severely hypoplastic   (MGI Ref ID J:66593)
    • ovary hypoplasia
    • testis hypoplasia
  • homeostasis/metabolism phenotype
  • decreased follicle stimulating hormone level
    • although mutant pituitaries appear histologically intact, immunoreactive FSH leves are virtually undetectable   (MGI Ref ID J:66593)
    • in contrast, pituitary ACTH, TSH and prolactin levels are comparable to those in wild-type pituitaries   (MGI Ref ID J:66593)
    • decreased circulating follicle stimulating hormone level
      • serum FSH levels are significantly reduced in both male and female mutants relative to wild-type controls   (MGI Ref ID J:66593)
  • decreased luteinizing hormone level
    • although mutant pituitaries appear histologically intact, immunoreactive LH leves are virtually undetectable   (MGI Ref ID J:66593)
    • decreased circulating luteinizing hormone level
      • serum LH levels are significantly reduced in male mutants relative to wild-type controls   (MGI Ref ID J:66593)

Nr5a1tm2Klp/Nr5a1tm2Klp Tg(Cga-cre)3Sac/0

        involves: 129P2/OlaHsd * C57BL/6J * SJL   (conditional)
  • reproductive system phenotype
  • abnormal sex gland morphology   (MGI Ref ID J:70412)
    • Leydig cell hypoplasia
      • at 8 weeks of age, mutant interstitial Leydig cells are severely hypoplastic   (MGI Ref ID J:70412)
      • in contrast, Sertoli cells and developing germ cells (including mature sperm) are relatively intact   (MGI Ref ID J:70412)
    • absent corpus luteum   (MGI Ref ID J:70412)
    • absent mature ovarian follicles
      • neither large preovulatory follicles nor corpora lutea are observed, similar to mice that are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
    • impaired ovarian folliculogenesis
      • mutant mice display absence of follicular maturation beyond the antral stage   (MGI Ref ID J:70412)
    • prostate gland hypoplasia
      • similar to that observed in mice that are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
    • seminal vesicle hypoplasia
      • similar to that observed in mice that are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
    • small ovary
      • although mutant ovaries are smaller than wild-type, they are significantly larger than those of mice that are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
      • decreased ovary weight
        • at 8 weeks of age, the weight of mutant ovaries (0.06 g/kg) is significantly lower than wild-type (0.47 g/kg), but higher than that of mice which are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac (0.01 g/kg)   (MGI Ref ID J:70412)
    • small testis
      • although mutant testes are smaller than wild-type, they are significantly larger than those of mice that are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
      • decreased testis weight
        • at 8 weeks of age, the weight of mutant testes (3.7 g/kg) is significantly lower than wild-type (7.9 g/kg), but significantly higher than that of mice which are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac (0.4 g/kg)   (MGI Ref ID J:70412)
  • absent sexual maturation
    • both male and female mutants show little evidence for secondary sexual maturation   (MGI Ref ID J:70412)
  • female infertility   (MGI Ref ID J:70412)
  • male infertility   (MGI Ref ID J:70412)
  • small gonad
    • both male and female mutants show a milder degree of (hypomorphic) hypogonadism than mice that are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
    • small ovary
      • although mutant ovaries are smaller than wild-type, they are significantly larger than those of mice that are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
      • decreased ovary weight
        • at 8 weeks of age, the weight of mutant ovaries (0.06 g/kg) is significantly lower than wild-type (0.47 g/kg), but higher than that of mice which are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac (0.01 g/kg)   (MGI Ref ID J:70412)
    • small testis
      • although mutant testes are smaller than wild-type, they are significantly larger than those of mice that are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
      • decreased testis weight
        • at 8 weeks of age, the weight of mutant testes (3.7 g/kg) is significantly lower than wild-type (7.9 g/kg), but significantly higher than that of mice which are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac (0.4 g/kg)   (MGI Ref ID J:70412)
  • uterus hypoplasia
    • similar to that observed in mice that are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
  • endocrine/exocrine gland phenotype
  • abnormal sex gland morphology   (MGI Ref ID J:70412)
    • Leydig cell hypoplasia
      • at 8 weeks of age, mutant interstitial Leydig cells are severely hypoplastic   (MGI Ref ID J:70412)
      • in contrast, Sertoli cells and developing germ cells (including mature sperm) are relatively intact   (MGI Ref ID J:70412)
    • absent corpus luteum   (MGI Ref ID J:70412)
    • absent mature ovarian follicles
      • neither large preovulatory follicles nor corpora lutea are observed, similar to mice that are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
    • impaired ovarian folliculogenesis
      • mutant mice display absence of follicular maturation beyond the antral stage   (MGI Ref ID J:70412)
    • prostate gland hypoplasia
      • similar to that observed in mice that are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
    • seminal vesicle hypoplasia
      • similar to that observed in mice that are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
    • small ovary
      • although mutant ovaries are smaller than wild-type, they are significantly larger than those of mice that are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
      • decreased ovary weight
        • at 8 weeks of age, the weight of mutant ovaries (0.06 g/kg) is significantly lower than wild-type (0.47 g/kg), but higher than that of mice which are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac (0.01 g/kg)   (MGI Ref ID J:70412)
    • small testis
      • although mutant testes are smaller than wild-type, they are significantly larger than those of mice that are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
      • decreased testis weight
        • at 8 weeks of age, the weight of mutant testes (3.7 g/kg) is significantly lower than wild-type (7.9 g/kg), but significantly higher than that of mice which are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac (0.4 g/kg)   (MGI Ref ID J:70412)
  • homeostasis/metabolism phenotype
  • decreased circulating follicle stimulating hormone level
    • mutant mice of both sexes show circulating FSH levels (4.5 ng/ml males, 5.2 ng/ml females) that are intermediate between those of wild-type mice (31.35 ng/ml males, 12.4 ng/ml females) and mice that are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac (1.2 ng/ml males, 2.7 ng/ml females)   (MGI Ref ID J:70412)
  • decreased circulating luteinizing hormone level
    • mutant males display a severe reduction in circulating LH levels similar to that observed in mice that are heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac   (MGI Ref ID J:70412)
  • behavior/neurological phenotype
  • abnormal sexual interaction
    • mutants do not engage in sexual activity   (MGI Ref ID J:70412)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Reproductive Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Nr5a1tm2Klp
Allele Name targeted mutation 2, Keith L Parker
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) F; Sf1Fl;
Mutation Made By Linda Washburn,   The Jackson Laboratory
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14TG2a
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Nr5a1, nuclear receptor subfamily 5, group A, member 1
Chromosome 2
Gene Common Name(s) AD4BP; Ad4BP/SF-1; ELP; FTZ1; FTZF1; Ftz-F1; Ftzf1; POF7; SF-1; SF1; SPGF8; SRXY3; adrenal 4-binding protein; fushi tarazu 1 factor homolog, (Drosophila); steroidogenic factor 1;
General Note Phenotypic Similarity to Human Syndrome: Hypogonadotropic Hypogonadism in mice heterozygous for Nr5a1tm2Klp and Nr5a1tm2.1Klp and hemizygous for Tg(Cga-cre)3Sac (J:66593)
Molecular Note A single loxP site was inserted into an intron 5' to the last coding exon, and a loxP site followed by a neomycin resistance cassette was inserted 3' to this exon. This mutation flanks sequences encoding motifs essential for transcriptional activity as well as the transcription termination and polyadenyulation sequences. [MGI Ref ID J:66593]

Genotyping

Genotyping Information

Genotyping Protocols

Nr5a1tm2Klp, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Luo X; Ikeda Y; Parker KL. 1994. A cell-specific nuclear receptor is essential for adrenal and gonadal development and sexual differentiation. Cell 77(4):481-90. [PubMed: 8187173]  [MGI Ref ID J:35576]

Additional References

Nr5a1tm2Klp related

Bakke M; Zhao L; Parker KL. 2001. Approaches to define the role of SF-1 at different levels of the hypothalamic-pituitary-steroidogenic organ axis. Mol Cell Endocrinol 179(1-2):33-7. [PubMed: 11420128]  [MGI Ref ID J:125681]

Buaas FW; Gardiner JR; Clayton S; Val P; Swain A. 2012. In vivo evidence for the crucial role of SF1 in steroid-producing cells of the testis, ovary and adrenal gland. Development 139(24):4561-70. [PubMed: 23136395]  [MGI Ref ID J:189958]

Freedman BD; Kempna PB; Carlone DL; Shah MS; Guagliardo NA; Barrett PQ; Gomez-Sanchez CE; Majzoub JA; Breault DT. 2013. Adrenocortical zonation results from lineage conversion of differentiated zona glomerulosa cells. Dev Cell 26(6):666-73. [PubMed: 24035414]  [MGI Ref ID J:204614]

Jeyasuria P; Ikeda Y; Jamin SP; Zhao L; De Rooij DG; Themmen AP; Behringer RR; Parker KL. 2004. Cell-specific knockout of steroidogenic factor 1 reveals its essential roles in gonadal function. Mol Endocrinol 18(7):1610-9. [PubMed: 15118069]  [MGI Ref ID J:91352]

Kato T; Esaki M; Matsuzawa A; Ikeda Y. 2012. NR5A1 is required for functional maturation of Sertoli cells during postnatal development. Reproduction 143(5):663-72. [PubMed: 22419830]  [MGI Ref ID J:185215]

Kim KW; Donato J Jr; Berglund ED; Choi YH; Kohno D; Elias CF; Depinho RA; Elmquist JK. 2012. FOXO1 in the ventromedial hypothalamus regulates energy balance. J Clin Invest 122(7):2578-89. [PubMed: 22653058]  [MGI Ref ID J:190474]

Kim KW; Jo YH; Zhao L; Stallings NR; Chua SC Jr; Parker KL. 2008. Steroidogenic factor 1 regulates expression of the cannabinoid receptor 1 in the ventromedial hypothalamic nucleus. Mol Endocrinol 22(8):1950-61. [PubMed: 18511494]  [MGI Ref ID J:138418]

Kim KW; Zhao L; Donato J Jr; Kohno D; Xu Y; Elias CF; Lee C; Parker KL; Elmquist JK. 2011. Steroidogenic factor 1 directs programs regulating diet-induced thermogenesis and leptin action in the ventral medial hypothalamic nucleus. Proc Natl Acad Sci U S A 108(26):10673-8. [PubMed: 21636788]  [MGI Ref ID J:173306]

Pelusi C; Ikeda Y; Zubair M; Parker KL. 2008. Impaired follicle development and infertility in female mice lacking steroidogenic factor 1 in ovarian granulosa cells. Biol Reprod 79(6):1074-83. [PubMed: 18703422]  [MGI Ref ID J:145805]

Roy A; Matzuk MM. 2006. Deconstructing mammalian reproduction: using knockouts to define fertility pathways. Reproduction 131(2):207-19. [PubMed: 16452715]  [MGI Ref ID J:108425]

Segal JP; Stallings NR; Lee CE; Zhao L; Socci N; Viale A; Harris TM; Soares MB; Childs G; Elmquist JK; Parker KL; Friedman JM. 2005. Use of laser-capture microdissection for the identification of marker genes for the ventromedial hypothalamic nucleus. J Neurosci 25(16):4181-8. [PubMed: 15843621]  [MGI Ref ID J:145979]

Wilson MJ; Jeyasuria P; Parker KL; Koopman P. 2005. The transcription factors steroidogenic factor-1 and SOX9 regulate expression of Vanin-1 during mouse testis development. J Biol Chem 280(7):5917-23. [PubMed: 15590666]  [MGI Ref ID J:96928]

Zhao L; Bakke M; Hanley NA; Majdic G; Stallings NR; Jeyasuria P; Parker KL. 2004. Tissue-specific knockouts of steroidogenic factor 1. Mol Cell Endocrinol 215(1-2):89-94. [PubMed: 15026179]  [MGI Ref ID J:88733]

Zhao L; Bakke M; Krimkevich Y; Cushman LJ; Parlow AF; Camper SA; Parker KL. 2001. Hypomorphic phenotype in mice with pituitary-specific knockout of steroidogenic factor 1. Genesis 30(2):65-9. [PubMed: 11416865]  [MGI Ref ID J:70412]

Zhao L; Bakke M; Krimkevich Y; Cushman LJ; Parlow AF; Camper SA; Parker KL. 2001. Steroidogenic factor 1 (SF1) is essential for pituitary gonadotrope function. Development 128(2):147-54. [PubMed: 11124111]  [MGI Ref ID J:66593]

Zhao L; Kim KW; Ikeda Y; Anderson KK; Beck L; Chase S; Tobet SA; Parker KL. 2008. Central nervous system-specific knockout of steroidogenic factor 1 results in increased anxiety-like behavior. Mol Endocrinol 22(6):1403-15. [PubMed: 18372344]  [MGI Ref ID J:136159]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice are bred.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000924 C57BL/6JEiJ
 
  Considerations for Choosing Controls
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Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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