Strain Name: |
B6Ei.129P2-Nr5a1tm2Klp/EiJ |
|---|---|
Stock Number: |
007041 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Nr5a1; Nr5a1tm2Klp; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Background Strain C57BL/6JEiJ Donor Strain 129P2/OlaHsd via E14TG2a ES cell line Donating Investigator Eva Eicher, The Jackson Laboratory Generation N12F2pN1 Strain Description
Mice homozygous for this floxed allele are viable and fertile. These mutant mice have loxP sites flanking the C-terminal coding exon. When bred to Cre-recombinase expressing mice, offspring will have a deletion of this exon in the cre expressing tissue(s). These floxed mice may be useful in studying steroidogenic factors and pituitary gonadotrope function.For example, when crossed to a strain expressing Cre recombinase in the anterior and intermediate lobes of the pituitary gland (see Stock No. 004426), this mutant mouse strain may be useful in studies of pituitary gonadotrope function.
Strain Development
A targeting vector was designed to place a loxP site upstream, and a loxP site and neomycin resistance cassette downstream of the C-terminal coding exon of the targted gene. The construct was electroporated into 129P2/OlaHsd-derived E14TG2a embryonic stem (ES) cells. Following blastocyst injection of correctly targeted ES cells, chimeric mice transmitted this "floxed" allele to produce mutant mice. These mutant mice have been backcrossed for at least 12 generations to C57BL/6JEiJ inbred mice (Stock No. 000924).
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Nr5a1tm2Klp | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Keith L Parker | ||
| Common Name(s) | F; | ||
| Mutation Made By | Linda Washburn, The Jackson Laboratory | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14TG2a | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Nr5a1, nuclear receptor subfamily 5, group A, member 1 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | AD4BP; Ad4BP/SF-1; ELP; FTZ1; FTZF1; Ftz-F1; Ftzf1; SF-1; SF1; adrenal 4-binding protein; fushi tarazu 1 factor homolog, (Drosophila); steroidogenic factor 1; | ||
| Molecular Note | A single loxP site was inserted into an intron 5' to the last coding exon, and a loxP site followed by a neomycin resistance cassette was inserted 3' to this exon. This mutation flanks sequences encoding motifs essential for transcriptional activity as well as the transcription termination and polyadenyulation sequences. [MGI Ref ID J:66593] | ||
Control Information
| Control | ||
|---|---|---|
| 000924 C57BL/6JEiJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Nr5a1tm2Klp
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, homozygous mice are bred. |
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Related Strains
Strains carrying Nr5a1tm2Klp allele
007042 D2.129P2(B6)-Nr5a1tm2Klp/EiJ View Strains carrying Nr5a1tm2Klp (1 strain)
003219 D2.129P2(B6)-Nr5a1tm1Klp/EiJ 006364 FVB-Tg(Nr5a1-cre)2Lowl/J
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Research Applications
This mouse can be used to support research in many areas including:
Research Tools
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)
Reproductive Biology Research (Cre-lox System)
References
Selected Reference(s)
Additional ReferencesLuo X; Ikeda Y; Parker KL. 1994. A cell-specific nuclear receptor is essential for adrenal and gonadal development and sexual differentiation. Cell 77(4):481-90. [PubMed: 8187173] [MGI Ref ID J:35576]
Price and Supply Information
| Strain Name: | B6Ei.129P2-Nr5a1tm2Klp/EiJ |
| Stock Number: | 007041 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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