Go to JAX® Mice Query Form

Strain Name:

DBA/2J-Gpnmb+/SjJ

Stock Number:

007048

Availability:

Repository- Live

Price and Supply Information

General Terms and Conditions

Genes & Alleles   Cdh23;   Cdh23ahl;   Gpnmb;   Gpnmb+;   Hc;   Hc0;

Product Information

Strain Details

Type JAX® GEMM® Strain - Coisogenic
Additional information on JAX® GEMM® Strains.
Type Inbred Strain
Additional information on Inbred Strains.
Specieslaboratory mouse
H2 Haplotyped

Appearance
dilute brown
Related Genotype: a/a Tyrp1b/Tyrp1b Myo5ad/Myo5ad

Important Note
This strain is homozygous for Cdh23ahl, the age related hearing loss 1 mutation, which on this background results in progressive hearing loss that is already severe by 3 months of age.

Strain Description
This coisogenic strain has a functional allele of Gpnmb. Homozygous mice do not develop elevated intraocular pressure or glaucoma, although they exhibit a mild iris stromal atrophy (ISA). This strain provides a genetically matched control for DBA/2J (Stock No. 000671). The inbred strain DBA/2J is homozygous for the glaucoma-related GpnmbR150X and Tyrp1isa mutations.

Strain Development
The wildtype allele of Gpnmb is present in the mutant strain DBA/2J-Dtnbp1sdy/J. The sandy mutation occurred in 1983 prior to the appearance of the GpnmbR150X mutation in the The Jackson Laboratory DBA/2J production colony. To generate this strain, mice from DBA/2J-Dtnbp1sdy/J were crossed to "modern" DBA/2J mice, and progeny were selected for the Gpnmb wildtype allele. The wildtype allele was backcrossed to DBA/2J for a minimum of six generations. Dr. Simon John of The Jackson Laboratory provided the colony to the Repository in 2007.

Gene & Allele Details

Allele Symbol Gpnmb+
Allele Name wild type
Gene Symbol and Name Gpnmb, glycoprotein (transmembrane) nmb
Chromosome 6
Gene Common Name(s) Dchil; HGFIN; NMB; Osteoactivin; dendritic cell associated heparan sulfate proteoglycans dependent integrin ligand; ipd; iris pigment dispersion;
 
Allele Symbol Cdh23ahl
Allele Name age related hearing loss 1
Common Name(s) Cdh23753A; mdfw;
Strain of OriginC57BL/6J
Gene Symbol and Name Cdh23, cadherin 23 (otocadherin)
Chromosome 10
Gene Common Name(s) 4930542A03Rik; DFNB12; DKFZp434P2350; FLJ00233; FLJ36499; KIAA1774; KIAA1812; MGC102761; RIKEN cDNA 4930542A03 gene; USH1D; W; age related hearing loss 1; ahl; bob; bobby; bus; bustling; mdfw; modifier of deaf waddler; neuroscience mutagenesis facility, 112; neuroscience mutagenesis facility, 181; neuroscience mutagenesis facility, 252; nmf112; nmf181; nmf252; v; waltzer;
Molecular Note Genetic complementation tests have shown allelism between the mdfw (modifier of deaf waddler) locus and the ahl locus. Further analysis has identified an association between ahl and a G to A transition at nucleotide position 753 of Cdh23. This hypomorphic allele causes in frame skipping of exon 7 and reduced message stability. Twenty-seven strains classified with ahl and carrying the 753A allele include: CD1, RBF/DnJ, PL/J, AKR/J, RF/J, BALB/cBy, A/WySnJ, P/J, SENCARA/PtJ, DBA/1J, ALS/LtJ, C58/J, C57BLKS/J, 129P1/ReJ, C57BR/cd, SKH2/J, BUB/Bn, MA/MyJ, LP/J, 129X1/SvJ, NOR/LtJ, A/J, C57BL/6, NOD/LyJ, DBA/2J, ALR/LtJ, C57L/J. Strains classified with ahl that DO NOT carry this mutation include: C3H/HeSnJ, I/LnJ,YBR/Ei, MRL/MpJ. [J:86905]
 
Allele Symbol Hc0
Allele Name deficient
Common Name(s) C5-; C5-d; C5-def; C5-deficient; hco;
Gene Symbol and Name Hc, hemolytic complement
Chromosome 2
Gene Common Name(s) C5; C5a; CPAMD4; FLJ17816; FLJ17822; He; MGC142298;
General Note

This is an allele characteristic of various inbred mouse strains including the following: A/HeJ, AKR/J, DBA/2J, NZB/B1NJ, SWR/J, B10.D2/oSnJ

Hc was identified as a candidate gene for Abhr2 in a microarray analysis of lung mRNA from A/J, C3H/HeJ, and (A/J x C3H/HeJ)F1 x A/J backcross animals. Hc genotype shows statistically significant correlation to allergen-induced bronchial hyperresponsive phenotype. The A/J allele contains a 2 bp deletion resulting in deficient Hc mRNA and protein production and is associated with susceptibility to allergen-induced bronchial hyperresponsiveness. (J:108211)

Molecular Note A 2 base "TA" deletion at positions 62 and 63 of an 83 base pair exon near the 5' end of the gene is found in the following mouse strains: A/HeJ, AKR/J, DBA/2J, NZB/B1NJ, SWR/J, B10.D2/oSnJ. The consequence of this deletion is the creation of a stop codon starting four bases after the deletion. A truncated product of 216 amino acids is predicted as a result although contradictory reports exist that a larger pro-C5 protein may be synthesized. Nevertheless, macrophages from mouse strains carrying this allele do not secrete complement 5. [J:23983]

Control Information

  Control
   000671 DBA/2J
 
  Considerations for Choosing Controls
  Control Pricing Information for JAX® GEMM® Strains

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony these mice can be bred as homozygotes.
Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying   Cdh23ahl allele
001137   129P1/ReJ
000690   129P3/J
002065   129T2/SvEmsJ
000691   129X1/SvJ
000646   A/J
000647   A/WySnJ
003070   ALR/LtJ
003072   ALS/LtJ
004502   B6;AKR-Lxl2/J
001026   BALB/cByJ
000653   BUB/BnJ
005494   C3.129S1(B6)-Grm1rcw/J
000664   C57BL/6J
004764   C57BL/6J-Cdh23v-8J/J
003129   C57BL/6J-Epha4rb-2J/J
004820   C57BL/6J-Kcne12J/J
004703   C57BL/6J-Nmf134/J
004811   C57BL/6J-nmf110/J
004812   C57BL/6J-nmf111/J
004747   C57BL/6J-nmf118/J
004656   C57BL/6J-nmf88/J
004391   C57BL/6J-Chr 13A/J/NaJ
004385   C57BL/6J-Chr 7A/J/NaJ
000662   C57BLKS/J
000667   C57BR/cdJ
000668   C57L/J
000669   C58/J
000657   CE/J
000670   DBA/1J
001140   DBA/1LacJ
000671   DBA/2J
002106   KK/HlJ
000675   LG/J
000676   LP/J
000677   MA/MyJ
001976   NOD/ShiLtJ
002050   NOR/LtJ
000679   P/J
002747   SENCARB/PtJ
002335   SKH2/J
003392   STOCK Crb1rd8/J
View Strains carrying   Cdh23ahl     (41 strains)

Strains carrying   Hc0 allele
000645   A/HeJ
000646   A/J
000647   A/WySnJ
000648   AKR/J
000460   B10.D2-Hc0 H2d H2-T18c/o2SnJ
000461   B10.D2-Hc0 H2d H2-T18c/oSnJ
000657   CE/J
000671   DBA/2J
001800   FVB/NJ
001491   FVB/NMob
000674   I/LnJ
001303   NOD.CB17-Prkdcscid/J
001976   NOD/ShiLtJ
000684   NZB/BlNJ
000682   RF/J
000688   ST/bJ
000689   SWR/J
View Strains carrying   Hc0     (17 strains)

Strains carrying other alleles of Cdh23
008288   B6(Cg)-Cdh23v-11J/J
002756   B6.CAST-Cdh23Ahl+/Kjn
002432   B6J x B6.C-H2bm1/ByJ-Cdh23v-J/J
002552   C57BL/6J-Cdh23v-2J/J
004764   C57BL/6J-Cdh23v-8J/J
004819   C57BL/6J-Cdh23v-9J/J
005016   CByJ;B6-Cdh23v-10J/J
000275   V/LeJ
View Strains carrying other alleles of Cdh23     (8 strains)

Strains carrying other alleles of Gpnmb
000671   DBA/2J
View Strains carrying other alleles of Gpnmb     (1 strain)

View Strains carrying other alleles of Hc     (5 strains)

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Sensorineural Research
Eye Defects (control)

Cdh23ahl related

Neurobiology Research
Vestibular and Hearing Defects (Age related hearing loss)

Sensorineural Research
Vestibular and Hearing Defects (Age related hearing loss)

Hc0 related

Immunology and Inflammation Research
Immunodeficiency (specific complement deficiency)

Research Tools
Immunology and Inflammation Research (specific complement deficiency) (C5 complement)

References

Selected Reference(s)

Howell GR; Libby RT; Marchant JK; Wilson LA; Cosma IM; Smith RS; Anderson MG; John SW. 2007. Absence of glaucoma in DBA/2J mice homozygous for wild-type versions of Gpnmb and Tyrp1. BMC Genet 8(1):45. [PubMed: 17608931]  [J:123136]

Additional References

Price and Supply Information

Strain Name: DBA/2J-Gpnmb+/SjJ
Stock Number: 007048

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:

*Pricing for Shipping Destination selected:

        USA, Canada and Mexico

Price(s) in US dollars ($)Genotype(s) Provided
Individual Mouse Price $201.10Homozygous for Gpnmb+
Pair $402.20Homozygous for Gpnmb+ x Homozygous for Gpnmb+

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423.
Usually shipped between four and eight weeks of age.
This strain is included in the Mouse Mutant Resource collection.
LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.
View Control Pricing Information for JAX® Strains.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

      Purchasing Information
      JAX® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Go to JAX® Mice Query Form

(2.13)