Strain Name:

B6;129S4-Glra2tm1Clc/J

Stock Number:

007065

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6;129-Glra2tm1Clc/J    (Changed: 07-JUN-07 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
Generation?pN1
Generation Definitions
 
Donating Investigator Connie Cepko,   Harvard Medical School, HHMI

Description
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. There is no identifiable phenotype in these mice. Expression is eliminated in the brain as determined by RT-PCR and Northern analyses, and in situ hybridization and immunofluorescence of spinal cord and retina. This strain may be helpful in studies to further delineate the role of this gene.

Development
An FRT-flanked PGK-neomycin drug resistance cassette was targeted to replace a 2.3 kb genomic segment containing exons 6 and 7 in a mouse Glra2-containing BAC. A NotI fragment from the BAC was electroporated into an 129S4/SvJae-derived J1 embryonic stem (ES) cell line. Homologously recombined ES cells were injected into blastocysts to generate chimeric animals. The foundation line was backcrossed to C57BL/6.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Glra2tm1Clc/Glra2tm1Clc

        involves: 129X1/SvJ
  • nervous system phenotype
  • abnormal single cell response
    • E17 cortical pyramidal neurons fail to respond to glycine or taurine in single cell patch clamp assays   (MGI Ref ID J:111414)
    • however, these cells respond normally to GABA and cortex morphology at P0 and in adults is indistinguishable from wild-type   (MGI Ref ID J:111414)
  • vision/eye phenotype
  • *normal* vision/eye phenotype
    • retinal morphology is similar to wild-type and no differences in electroreinograms are detected   (MGI Ref ID J:111414)
  • reproductive system phenotype
  • *normal* reproductive system phenotype
    • fertile with normal breeding behavior   (MGI Ref ID J:111414)
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • normal gait and feeding behavior   (MGI Ref ID J:111414)

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Glra2tm1Clc
Allele Name targeted mutation 1, Constance L Cepko
Allele Type Targeted (Null/Knockout)
Mutation Made By Connie Cepko,   Harvard Medical School, HHMI
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Glra2, glycine receptor, alpha 2 subunit
Chromosome X
Gene Common Name(s) GLR; RNNEOGLY;
Molecular Note A vector was designed to replace exons 6 and 7 with an FRT-flanked PGK-neomycin cassette. These exons encode two transmembrane domains and a large portion of its ligand binding domain. Alternative splicing of exon 5 with exon 8 would result in an immediate premature stop codon and should lead to a nonsense-mediated degradation of the mRNA. RT-PCR, Northern blot and in situ hybridization assays were performed to confirm absence of transcript in mutants. [MGI Ref ID J:111414]

Genotyping

Genotyping Information

Genotyping Protocols

Glra2tm1Clc, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Young-Pearse TL; Ivic L; Kriegstein AR; Cepko CL. 2006. Characterization of mice with targeted deletion of glycine receptor alpha 2. Mol Cell Biol 26(15):5728-34. [PubMed: 16847326]  [MGI Ref ID J:111414]

Additional References

Glra2tm1Clc related

Majumdar S; Weiss J; Wassle H. 2009. Glycinergic input of widefield, displaced amacrine cells of the mouse retina. J Physiol 587(Pt 15):3831-49. [PubMed: 19528249]  [MGI Ref ID J:176536]

Nobles RD; Zhang C; Muller U; Betz H; McCall MA. 2012. Selective glycine receptor alpha2 subunit control of crossover inhibition between the on and off retinal pathways. J Neurosci 32(10):3321-32. [PubMed: 22399754]  [MGI Ref ID J:182733]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as heterozygotes or homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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