Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6;129-Glra2tm1Clc/J    (Changed: 07-JUN-07 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation ?pN1 Generation Definitions   Donating Investigator Connie Cepko,   Harvard Medical School, HHMI

Description
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. There is no identifiable phenotype in these mice. Expression is eliminated in the brain as determined by RT-PCR and Northern analyses, and in situ hybridization and immunofluorescence of spinal cord and retina. This strain may be helpful in studies to further delineate the role of this gene.

Development
An FRT-flanked PGK-neomycin drug resistance cassette was targeted to replace a 2.3 kb genomic segment containing exons 6 and 7 in a mouse Glra2-containing BAC. A NotI fragment from the BAC was electroporated into an 129S4/SvJae-derived J1 embryonic stem (ES) cell line. Homologously recombined ES cells were injected into blastocysts to generate chimeric animals. The foundation line was backcrossed to C57BL/6.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Glra2^tm1Clc/Glra2^tm1Clc

        involves: 129X1/SvJ

• nervous system phenotype
• abnormal single cell response
  • E17 cortical pyramidal neurons fail to respond to glycine or taurine in single cell patch clamp assays   (MGI Ref ID J:111414)
  • however, these cells respond normally to GABA and cortex morphology at P0 and in adults is indistinguishable from wild-type   (MGI Ref ID J:111414)
• vision/eye phenotype
• *normal* vision/eye phenotype
  • retinal morphology is similar to wild-type and no differences in electroreinograms are detected   (MGI Ref ID J:111414)
• reproductive system phenotype
• *normal* reproductive system phenotype
  • fertile with normal breeding behavior   (MGI Ref ID J:111414)
• behavior/neurological phenotype
• *normal* behavior/neurological phenotype
  • normal gait and feeding behavior   (MGI Ref ID J:111414)

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Glra2tm1Clc
Allele Name		targeted mutation 1, Constance L Cepko
Allele Type		Targeted (knock-out)
Mutation Made By		Connie Cepko,   Harvard Medical School, HHMI
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Glra2, glycine receptor, alpha 2 subunit
Chromosome		X
Gene Common Name(s)		GLR; RNNEOGLY;
Molecular Note		A vector was designed to replace exons 6 and 7 with an FRT-flanked PGK-neomycin cassette. These exons encode two transmembrane domains and a large portion of its ligand binding domain. Alternative splicing of exon 5 with exon 8 would result in an immediate premature stop codon and should lead to a nonsense-mediated degradation of the mRNA. RT-PCR, Northern blot and in situ hybridization assays were performed to confirm absence of transcript in mutants. [MGI Ref ID J:111414]

Genotyping

Genotyping Information

Genotyping Protocols

Glra2^tm1Clc, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Young-Pearse TL; Ivic L; Kriegstein AR; Cepko CL. 2006. Characterization of mice with targeted deletion of glycine receptor alpha 2. Mol Cell Biol 26(15):5728-34. [PubMed: 16847326]  [MGI Ref ID J:111414]

Additional References

Glra2^tm1Clc related

Majumdar S; Weiss J; Wassle H. 2009. Glycinergic input of widefield, displaced amacrine cells of the mouse retina. J Physiol 587(Pt 15):3831-49. [PubMed: 19528249]  [MGI Ref ID J:176536]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred as heterozygotes or homozygotes.

Purchasing information

Pricing, Supply Level & Notes, Controls

General Supply Notes

• This strain is included in the Induced Mutant Resource Colony collection.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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