Strain Name: |
B6.SJL-Tg(Crx-GFP,-ALPP)1Clc/J |
|---|---|
Stock Number: |
007066 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.SJL-Tg(Crx-GFP/IRES-ALPP)1Clc/J (Changed: 17-MAY-07
) |
| Genes & Alleles | ALPP; Tg(Crx-GFP,-ALPP)1Clc; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Connie Cepko, Harvard Medical School, HHMI Generation ?+N1 (31-DEC-07) Strain Description
Heterozygotes are viable and fertile. Expression of human placental alkaline phosphatase (PLAP) and GFP recapitulates that of the endogenous Crx promoter in the retina at embryonic time points as early as embryonic day 12.5 (E12.5) and in the retina of the adult mouse. This line also shows a more sensitive detection of Crx expression in the optic nerve of the developing retina. PLAP (but not GFP) expression is also found in fresh frozen pineal gland samples collected from adult mice.Strain Development
A loxP-flanked kanamycin/neomycin drug resistance cassette and a GFP/IRES/PLAP fragment were targeted to replace the first exon in a mouse Crx-containing BAC by ET-cloning. Cre was activated in the EL250 bacterial host strain with arabinose to remove the floxed kanamycin/neomycin segment, and the resultant BAC was digested with NotI to generate a transgenic fragment. The fragment was electroporated into an SJL-derived embryonic stem (ES) cell line to create a strain in which the Crx promoter drives expression of the GFP/IRES/PLAP transgene. The chimeric foundation line was backcrossed 10 times to C57BL/6 by the donating laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Crx-GFP,-ALPP)1Clc | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Constance L Cepko | ||
| Common Name(s) | Crx GFPAP; | ||
| Strain of Origin | SJL | ||
| ES Cell Line Strain | 129 | ||
| Expressed Gene | ALPP, alkaline phosphatase, placental (Regan isozyme), human | ||
| Molecular Note | A loxP-flanked kanamycin/neomycin drug resistance cassette and a GFP/IRES/PLAP fragment were targeted to replace the first exon of the mouse Crx gene in a BAC clone by ET-cloning. Cre was activated in the EL250 bacterial host strain with arabinose to remove the floxed kanamycin/neomycin segment, and the resultant BAC was digested with NotI to generate a transgenic fragment. [MGI Ref ID J:120988] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Crx-GFP,-ALPP)1Clc
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying other alleles of ALPP
006882 STOCK Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe/J 006613 STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J 003919 STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J 005105 STOCK Tg(Chx10-EGFP/cre-ALPP)2Clc/J View Strains carrying other alleles of ALPP (4 strains)
Additional Web Information
Congenic Nomenclature
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
Sensorineural Research
Eye Defects
References
Selected Reference(s)
Cepko CL. 2007. A mouse expressing a Crx-driven dual reporter transgene MGI Direct Data Submission :. [MGI Ref ID J:120988]
Price and Supply Information
| Strain Name: | B6.SJL-Tg(Crx-GFP,-ALPP)1Clc/J |
| Stock Number: | 007066 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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