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| These transgenic mice express Green Fluorescent Protein (GFP) under the direction of the human ubiqutin C promoter. Certain hematapoetic cell types display distinct expression levels of GFP, allowing identification of different cells types by FACS analysis. This mutant mouse strain represents a useful tool in studies related to hematopoetic cell differentiation and in vivo tracking of leukocytes. This is the same transgene found in (Stock No. 004353), which is on the C57BL/6J background. | |||||||||||||||||||
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Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 09-FEB-10 Species laboratory mouse Generation N6+N4F8 (29-JUN-11)
Generation DefinitionsDonating Investigator IMR Colony, The Jackson Laboratory Description
These transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the direction of the human ubiqutin C promoter. Mice hemizygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice express EGFP in all tissues examined. Certain hematopoetic cell types display distinct expression levels of EGFP, allowing identification of different cells types by FACS analysis. EGFP expression is uniform within a cell type lineage and remains constant throughout development. T cells have a 2-fold higher EGFP expression than CD19+B220+ B cells or peripheral blood cells. Leukocytes and red blood cells from homozygous mice fluoresce at approximately twice the level of cells from hemizygous mice. This mutant mouse strain represents a useful tool in studies related to hematopoetic cell differentiation and in vivo tracking of leukocytes.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A transgenic construct containing an enhanced green fluorescent protein (EGFP) open reading frame under the control of the human ubiqutin C promoter was microinjected into fertilized C57BL/6 oocytes. Mice were screened for genomic integration of the transgene by PCR and for GFP expression in peripheral blood leukocytes. The resulting female mouse exhibiting the desired phenotype was bred to a male C57BL/6J mouse. F1 progeny from this cross that expressed GFP were intercrossed to produce homozygotes. The mice were then backcrossed to BALB/cBy for 5 generations before arriving at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 001026 BALB/cByJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
013115 B6.Cg-Rag1tm1Mom Tg(UBC-GFP)30Scha/J 004353 C57BL/6-Tg(UBC-GFP)30Scha/J
007179 129S.Cg-Tg(UBC-cre/ERT2)1Ejb/J 017614 B6(Cg)-Tyrc-2J Tg(UBC-mCherry)1Phbs/J 006259 B6.Cg-Pepcb Ptprca Tg(UBC-scFv)2Nemz/J 015805 B6.Cg-Tg(UBC-GFP,-TVA)1Clc/J 015806 B6.Cg-Tg(UBC-GFP,-TVA)2Clc/J 015807 B6.Cg-Tg(UBC-GFP,-TVA)3Clc/J 015808 B6.Cg-Tg(UBC-TVA)1Clc/J 008085 B6.Cg-Tg(UBC-cre/ERT2)1Ejb/J 007001 B6;129S-Tg(UBC-cre/ERT2)1Ejb/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedImmunology, Inflammation and Autoimmunity Research
Research Tools
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Hematological Research
Immunology and Inflammation Research
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(UBC-GFP)30Scha | ||
|---|---|---|---|
| Allele Name | transgene insertion 30, Brian Schaefer | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Tg(UBCGFP)30Scha; UBC-GFP; UBI-EGFP; UBI-GFP; Ub-GFP; uGFP; | ||
| Mutation Made By | Brian Schaefer, Uniformed Services Univ. Health Sciences | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | Certain hematopoietic cell types display distinct expression levels of GFP. Expression is uniform within a cell type lineage and remains constant throughout development. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | UBC, ubiquitin C, human | ||
| Gene Symbol and Name | Tg(UBC-GFP)30Scha, transgene insertion 30, Brian Schaefer | ||
| Chromosome | UN | ||
| Gene Common Name(s) | UBC-GFP; UBI-EGFP; UBI-GFP; uGFP; | ||
| General Note | Homozygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These mice express GFP in all tissues examined. Expression levels vary between certain hematapoetic cell types. GFP expression is uniform within a cell type lineage and remains constant throughout development. T cells have a 2-fold higher GFP expression than CD19+B220+ B cells or peripheral blood cells. Leukocytes and red blood cells from homozygous transgenic mice fluoresce at approximately twice the level of cells from hemizygous mice. | ||
| Molecular Note | The transgene contains an enhanced green fluorescent protein open reading frame under the control of the human ubiquitin C promoter. [MGI Ref ID J:93490] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Schaefer BC; Schaefer ML; Kappler JW; Marrack P; Kedl RM. 2001. Observation of antigen-dependent CD8+ T-cell/ dendritic cell interactions in vivo. Cell Immunol 214(2):110-22. [PubMed: 12088410] [MGI Ref ID J:93490]
Additional References
Tg(UBC-GFP)30Scha relatedAkk AM; Simmons PM; Chan HW; Agapov E; Holtzman MJ; Grayson MH; Pham CT. 2008. Dipeptidyl peptidase I-dependent neutrophil recruitment modulates the inflammatory response to sendai virus infection. J Immunol 180(5):3535-42. [PubMed: 18292580] [MGI Ref ID J:131520]
Allen CD; Okada T; Tang HL; Cyster JG. 2007. Imaging of germinal center selection events during affinity maturation. Science 315(5811):528-31. [PubMed: 17185562] [MGI Ref ID J:118931]
Bajenoff M; Egen JG; Koo LY; Laugier JP; Brau F; Glaichenhaus N; Germain RN. 2006. Stromal cell networks regulate lymphocyte entry, migration, and territoriality in lymph nodes. Immunity 25(6):989-1001. [PubMed: 17112751] [MGI Ref ID J:116602]
Bajenoff M; Germain RN. 2009. B-cell follicle development remodels the conduit system and allows soluble antigen delivery to follicular dendritic cells. Blood 114(24):4989-97. [PubMed: 19713459] [MGI Ref ID J:155014]
Bajenoff M; Glaichenhaus N; Germain RN. 2008. Fibroblastic reticular cells guide T lymphocyte entry into and migration within the splenic T cell zone. J Immunol 181(6):3947-54. [PubMed: 18768849] [MGI Ref ID J:139103]
Berretta F; St-Pierre J; Piccirillo CA; Stevenson MM. 2011. IL-2 contributes to maintaining a balance between CD4+Foxp3+ regulatory T cells and effector CD4+ T cells required for immune control of blood-stage malaria infection. J Immunol 186(8):4862-71. [PubMed: 21389253] [MGI Ref ID J:172463]
Britschgi MR; Favre S; Luther SA. 2010. CCL21 is sufficient to mediate DC migration, maturation and function in the absence of CCL19. Eur J Immunol 40(5):1266-71. [PubMed: 20201039] [MGI Ref ID J:160954]
Byrne SN; Limon-Flores AY; Ullrich SE. 2008. Mast Cell Migration from the Skin to the Draining Lymph Nodes upon Ultraviolet Irradiation Represents a Key Step in the Induction of Immune Suppression. J Immunol 180(7):4648-55. [PubMed: 18354188] [MGI Ref ID J:133390]
Carmi Y; Dotan S; Rider P; Kaplanov I; White MR; Baron R; Abutbul S; Huszar M; Dinarello CA; Apte RN; Voronov E. 2013. The Role of IL-1beta in the Early Tumor Cell-Induced Angiogenic Response. J Immunol 190(7):3500-9. [PubMed: 23475218] [MGI Ref ID J:194907]
Chamoto K; Gibney BC; Lee GS; Lin M; Collings-Simpson D; Voswinckel R; Konerding MA; Tsuda A; Mentzer SJ. 2012. CD34+ progenitor to endothelial cell transition in post-pneumonectomy angiogenesis. Am J Respir Cell Mol Biol 46(3):283-9. [PubMed: 21921238] [MGI Ref ID J:194584]
Chen KA; Cruz PE; Lanuto DJ; Flotte TR; Borchelt DR; Srivastava A; Zhang J; Steindler DA; Zheng T. 2011. Cellular fusion for gene delivery to SCA1 affected Purkinje neurons. Mol Cell Neurosci 47(1):61-70. [PubMed: 21420496] [MGI Ref ID J:177962]
Clambey ET; McNamee EN; Westrich JA; Glover LE; Campbell EL; Jedlicka P; de Zoeten EF; Cambier JC; Stenmark KR; Colgan SP; Eltzschig HK. 2012. Hypoxia-inducible factor-1 alpha-dependent induction of FoxP3 drives regulatory T-cell abundance and function during inflammatory hypoxia of the mucosa. Proc Natl Acad Sci U S A 109(41):E2784-93. [PubMed: 22988108] [MGI Ref ID J:190110]
Claus C; Riether C; Schurch C; Matter MS; Hilmenyuk T; Ochsenbein AF. 2012. CD27 signaling increases the frequency of regulatory T cells and promotes tumor growth. Cancer Res 72(14):3664-76. [PubMed: 22628427] [MGI Ref ID J:189295]
Cortez-Retamozo V; Etzrodt M; Newton A; Rauch PJ; Chudnovskiy A; Berger C; Ryan RJ; Iwamoto Y; Marinelli B; Gorbatov R; Forghani R; Novobrantseva TI; Koteliansky V; Figueiredo JL; Chen JW; Anderson DG; Nahrendorf M; Swirski FK; Weissleder R; Pittet MJ. 2012. Origins of tumor-associated macrophages and neutrophils. Proc Natl Acad Sci U S A 109(7):2491-6. [PubMed: 22308361] [MGI Ref ID J:182625]
Cortez-Retamozo V; Etzrodt M; Newton A; Ryan R; Pucci F; Sio SW; Kuswanto W; Rauch PJ; Chudnovskiy A; Iwamoto Y; Kohler R; Marinelli B; Gorbatov R; Wojtkiewicz G; Panizzi P; Mino-Kenudson M; Forghani R; Figueiredo JL; Chen JW; Xavier R; Swirski FK; Nahrendorf M; Weissleder R; Pittet MJ. 2013. Angiotensin II Drives the Production of Tumor-Promoting Macrophages. Immunity 38(2):296-308. [PubMed: 23333075] [MGI Ref ID J:193393]
Croker BA; O'Donnell JA; Nowell CJ; Metcalf D; Dewson G; Campbell KJ; Rogers KL; Hu Y; Smyth GK; Zhang JG; White M; Lackovic K; Cengia LH; O'Reilly LA; Bouillet P; Cory S; Strasser A; Roberts AW. 2011. Fas-mediated neutrophil apoptosis is accelerated by Bid, Bak, and Bax and inhibited by Bcl-2 and Mcl-1. Proc Natl Acad Sci U S A 108(32):13135-40. [PubMed: 21768356] [MGI Ref ID J:176025]
Dai M; Yang Y; Omelchenko I; Nuttall AL; Kachelmeier A; Xiu R; Shi X. 2010. Bone marrow cell recruitment mediated by inducible nitric oxide synthase/stromal cell-derived factor-1alpha signaling repairs the acoustically damaged cochlear blood-labyrinth barrier. Am J Pathol 177(6):3089-99. [PubMed: 21057001] [MGI Ref ID J:167627]
Deguine J; Breart B; Lemaitre F; Di Santo JP; Bousso P. 2010. Intravital Imaging Reveals Distinct Dynamics for Natural Killer and CD8(+) T Cells during Tumor Regression. Immunity 33(4):632-44. [PubMed: 20951068] [MGI Ref ID J:165485]
Derecki NC; Cardani AN; Yang CH; Quinnies KM; Crihfield A; Lynch KR; Kipnis J. 2010. Regulation of learning and memory by meningeal immunity: a key role for IL-4. J Exp Med 207(5):1067-80. [PubMed: 20439540] [MGI Ref ID J:160932]
Derecki NC; Cronk JC; Lu Z; Xu E; Abbott SB; Guyenet PG; Kipnis J. 2012. Wild-type microglia arrest pathology in a mouse model of Rett syndrome. Nature 484(7392):105-9. [PubMed: 22425995] [MGI Ref ID J:183878]
Dudley AC; Udagawa T; Melero-Martin JM; Shih SC; Curatolo A; Moses MA; Klagsbrun M. 2010. Bone marrow is a reservoir for proangiogenic myelomonocytic cells but not endothelial cells in spontaneous tumors. Blood 116(17):3367-71. [PubMed: 20453162] [MGI Ref ID J:165908]
Dutta P; Courties G; Wei Y; Leuschner F; Gorbatov R; Robbins CS; Iwamoto Y; Thompson B; Carlson AL; Heidt T; Majmudar MD; Lasitschka F; Etzrodt M; Waterman P; Waring MT; Chicoine AT; van der Laan AM; Niessen HW; Piek JJ; Rubin BB; Butany J; Stone JR; Katus HA; Murphy SA; Morrow DA; Sabatine MS; Vinegoni C; Moskowitz MA; Pittet MJ; Libby P; Lin CP; Swirski FK; Weissleder R; Nahrendorf M. 2012. Myocardial infarction accelerates atherosclerosis. Nature 487(7407):325-9. [PubMed: 22763456] [MGI Ref ID J:186697]
Fancke B; Suter M; Hochrein H; O'Keeffe M. 2008. M-CSF: a novel plasmacytoid and conventional dendritic cell poietin. Blood 111(1):150-9. [PubMed: 17916748] [MGI Ref ID J:130107]
Fee D; Crumbaugh A; Jacques T; Herdrich B; Sewell D; Auerbach D; Piaskowski S; Hart MN; Sandor M; Fabry Z. 2003. Activated/effector CD4+ T cells exacerbate acute damage in the central nervous system following traumatic injury. J Neuroimmunol 136(1-2):54-66. [PubMed: 12620643] [MGI Ref ID J:119181]
Ford MS; Zhang ZX; Chen W; Zhang L. 2006. Double-negative T regulatory cells can develop outside the thymus and do not mature from CD8+ T cell precursors. J Immunol 177(5):2803-9. [PubMed: 16920915] [MGI Ref ID J:139556]
Furuhashi M; Fucho R; Gorgun CZ; Tuncman G; Cao H; Hotamisligil GS. 2008. Adipocyte/macrophage fatty acid-binding proteins contribute to metabolic deterioration through actions in both macrophages and adipocytes in mice. J Clin Invest 118(7):2640-50. [PubMed: 18551191] [MGI Ref ID J:137702]
Gerner MY; Kastenmuller W; Ifrim I; Kabat J; Germain RN. 2012. Histo-cytometry: a method for highly multiplex quantitative tissue imaging analysis applied to dendritic cell subset microanatomy in lymph nodes. Immunity 37(2):364-76. [PubMed: 22863836] [MGI Ref ID J:187382]
Grigorova IL; Panteleev M; Cyster JG. 2010. Lymph node cortical sinus organization and relationship to lymphocyte egress dynamics and antigen exposure. Proc Natl Acad Sci U S A 107(47):20447-52. [PubMed: 21059923] [MGI Ref ID J:166592]
Grigorova IL; Schwab SR; Phan TG; Pham TH; Okada T; Cyster JG. 2009. Cortical sinus probing, S1P1-dependent entry and flow-based capture of egressing T cells. Nat Immunol 10(1):58-65. [PubMed: 19060900] [MGI Ref ID J:142339]
He S; Kim I; Lim MS; Morrison SJ. 2011. Sox17 expression confers self-renewal potential and fetal stem cell characteristics upon adult hematopoietic progenitors. Genes Dev 25(15):1613-27. [PubMed: 21828271] [MGI Ref ID J:174416]
Hisatomi T; Nakazawa T; Noda K; Almulki L; Miyahara S; Nakao S; Ito Y; She H; Kohno R; Michaud N; Ishibashi T; Hafezi-Moghadam A; Badley AD; Kroemer G; Miller JW. 2008. HIV protease inhibitors provide neuroprotection through inhibition of mitochondrial apoptosis in mice. J Clin Invest 118(6):2025-38. [PubMed: 18497877] [MGI Ref ID J:137728]
Houston EG Jr; Higdon LE; Fink PJ. 2011. Recent thymic emigrants are preferentially incorporated only into the depleted T-cell pool. Proc Natl Acad Sci U S A 108(13):5366-71. [PubMed: 21402911] [MGI Ref ID J:171238]
Hsu HC; Yang P; Wang J; Wu Q; Myers R; Chen J; Yi J; Guentert T; Tousson A; Stanus AL; Le TV; Lorenz RG; Xu H; Kolls JK; Carter RH; Chaplin DD; Williams RW; Mountz JD. 2008. Interleukin 17-producing T helper cells and interleukin 17 orchestrate autoreactive germinal center development in autoimmune BXD2 mice. Nat Immunol 9(2):166-75. [PubMed: 18157131] [MGI Ref ID J:131634]
Hu Z; Van Rooijen N; Yang YG. 2011. Macrophages prevent human red blood cell reconstitution in immunodeficient mice. Blood 118(22):5938-46. [PubMed: 21926352] [MGI Ref ID J:178783]
Hugues S; Scholer A; Boissonnas A; Nussbaum A; Combadiere C; Amigorena S; Fetler L. 2007. Dynamic imaging of chemokine-dependent CD8(+) T cell help for CD8(+) T cell responses. Nat Immunol 8(9):921-30. [PubMed: 17660821] [MGI Ref ID J:124274]
Hwang IY; Park C; Harrison K; Kehrl JH. 2009. TLR4 signaling augments B lymphocyte migration and overcomes the restriction that limits access to germinal center dark zones. J Exp Med 206(12):2641-57. [PubMed: 19917774] [MGI Ref ID J:155452]
Josefsson EC; James C; Henley KJ; Debrincat MA; Rogers KL; Dowling MR; White MJ; Kruse EA; Lane RM; Ellis S; Nurden P; Mason KD; O'Reilly LA; Roberts AW; Metcalf D; Huang DC; Kile BT. 2011. Megakaryocytes possess a functional intrinsic apoptosis pathway that must be restrained to survive and produce platelets. J Exp Med 208(10):2017-31. [PubMed: 21911424] [MGI Ref ID J:177288]
Joyal JS; Sitaras N; Binet F; Rivera JC; Stahl A; Zaniolo K; Shao Z; Polosa A; Zhu T; Hamel D; Djavari M; Kunik D; Honore JC; Picard E; Zabeida A; Varma DR; Hickson G; Mancini J; Klagsbrun M; Costantino S; Beausejour C; Lachapelle P; Smith LE; Chemtob S;Sapieha P. 2011. Ischemic neurons prevent vascular regeneration of neural tissue by secreting semaphorin 3A. Blood 117(22):6024-35. [PubMed: 21355092] [MGI Ref ID J:177800]
Kitano M; Moriyama S; Ando Y; Hikida M; Mori Y; Kurosaki T; Okada T. 2011. Bcl6 protein expression shapes pre-germinal center B cell dynamics and follicular helper T cell heterogeneity. Immunity 34(6):961-72. [PubMed: 21636294] [MGI Ref ID J:174014]
Kubovcakova L; Lundberg P; Grisouard J; Hao-Shen H; Romanet V; Andraos R; Murakami M; Dirnhofer S; Wagner KU; Radimerski T; Skoda RC. 2013. Differential effects of hydroxyurea and INC424 on mutant allele burden and myeloproliferative phenotype in a JAK2-V617F polycythemia vera mouse model. Blood 121(7):1188-99. [PubMed: 23264594] [MGI Ref ID J:194600]
Kurkjian C; Hollifield M; Lines JL; Rogosky A; Empey KM; Qureshi M; Brown SA; Garvy BA. 2012. Alveolar Macrophages in Neonatal Mice Are Inherently Unresponsive to Pneumocystis murina Infection. Infect Immun 80(8):2835-46. [PubMed: 22665378] [MGI Ref ID J:186637]
Lambertsen KL; Clausen BH; Babcock AA; Gregersen R; Fenger C; Nielsen HH; Haugaard LS; Wirenfeldt M; Nielsen M; Dagnaes-Hansen F; Bluethmann H; Faergeman NJ; Meldgaard M; Deierborg T; Finsen B. 2009. Microglia protect neurons against ischemia by synthesis of tumor necrosis factor. J Neurosci 29(5):1319-30. [PubMed: 19193879] [MGI Ref ID J:155658]
Lannutti BJ; Epp A; Roy J; Chen J; Josephson NC. 2009. Incomplete restoration of Mpl expression in the mpl-/- mouse produces partial correction of the stem cell-repopulating defect and paradoxical thrombocytosis. Blood 113(8):1778-85. [PubMed: 18796624] [MGI Ref ID J:145455]
Lee JC; Seong J; Kim SH; Lee SJ; Cho YJ; An J; Nam DH; Joo KM; Cha CI. 2012. Replacement of microglial cells using Clodronate liposome and bone marrow transplantation in the central nervous system of SOD1(G93A) transgenic mice as an in vivo model of amyotrophic lateral sclerosis. Biochem Biophys Res Commun 418(2):359-65. [PubMed: 22269142] [MGI Ref ID J:181281]
Li H; Sorenson AL; Poczobutt J; Amin J; Joyal T; Sullivan T; Crossno JT Jr; Weiser-Evans MC; Nemenoff RA. 2011. Activation of PPARgamma in myeloid cells promotes lung cancer progression and metastasis. PLoS One 6(12):e28133. [PubMed: 22145026] [MGI Ref ID J:182274]
Lu KT; Kanno Y; Cannons JL; Handon R; Bible P; Elkahloun AG; Anderson SM; Wei L; Sun H; O'Shea JJ; Schwartzberg PL. 2011. Functional and epigenetic studies reveal multistep differentiation and plasticity of in vitro-generated and in vivo-derived follicular T helper cells. Immunity 35(4):622-32. [PubMed: 22018472] [MGI Ref ID J:177860]
Metcalf D; Ng A; Mifsud S; Di Rago L. 2010. Multipotential hematopoietic blast colony-forming cells exhibit delays in self-generation and lineage commitment. Proc Natl Acad Sci U S A 107(37):16257-61. [PubMed: 20805490] [MGI Ref ID J:164367]
Mionnet C; Sanos SL; Mondor I; Jorquera A; Laugier JP; Germain RN; Bajenoff M. 2011. High endothelial venules as traffic control points maintaining lymphocyte population homeostasis in lymph nodes. Blood 118(23):6115-22. [PubMed: 21937697] [MGI Ref ID J:179107]
Muller AJ; Filipe-Santos O; Eberl G; Aebischer T; Spath GF; Bousso P. 2012. CD4+ T cells rely on a cytokine gradient to control intracellular pathogens beyond sites of antigen presentation. Immunity 37(1):147-57. [PubMed: 22727490] [MGI Ref ID J:187413]
Naik SH; Sathe P; Park HY; Metcalf D; Proietto AI; Dakic A; Carotta S; O'Keeffe M; Bahlo M; Papenfuss A; Kwak JY; Wu L; Shortman K. 2007. Development of plasmacytoid and conventional dendritic cell subtypes from single precursor cells derived in vitro and in vivo. Nat Immunol 8(11):1217-26. [PubMed: 17922015] [MGI Ref ID J:126316]
Ng AP; Loughran SJ; Metcalf D; Hyland CD; de Graaf CA; Hu Y; Smyth GK; Hilton DJ; Kile BT; Alexander WS. 2011. Erg is required for self-renewal of hematopoietic stem cells during stress hematopoiesis in mice. Blood 118(9):2454-61. [PubMed: 21673349] [MGI Ref ID J:177792]
Nguyen V; Luzina I; Rus H; Tegla C; Chen C; Rus V. 2012. IL-21 promotes lupus-like disease in chronic graft-versus-host disease through both CD4 T cell- and B cell-intrinsic mechanisms. J Immunol 189(2):1081-93. [PubMed: 22723520] [MGI Ref ID J:189795]
Oliver PM; Cao X; Worthen GS; Shi P; Briones N; MacLeod M; White J; Kirby P; Kappler J; Marrack P; Yang B. 2006. Ndfip1 protein promotes the function of itch ubiquitin ligase to prevent T cell activation and T helper 2 cell-mediated inflammation. Immunity 25(6):929-40. [PubMed: 17137798] [MGI Ref ID J:116617]
Otahal P; Knowles BB; Tevethia SS; Schell TD. 2007. Anti-CD40 conditioning enhances the T(CD8) response to a highly tolerogenic epitope and subsequent immunotherapy of simian virus 40 T antigen-induced pancreatic tumors. J Immunol 179(10):6686-95. [PubMed: 17982058] [MGI Ref ID J:131746]
Otero K; Turnbull IR; Poliani PL; Vermi W; Cerutti E; Aoshi T; Tassi I; Takai T; Stanley SL; Miller M; Shaw AS; Colonna M. 2009. Macrophage colony-stimulating factor induces the proliferation and survival of macrophages via a pathway involving DAP12 and beta-catenin. Nat Immunol 10(7):734-43. [PubMed: 19503107] [MGI Ref ID J:150133]
Pagano MB; Bartoli MA; Ennis TL; Mao D; Simmons PM; Thompson RW; Pham CT. 2007. Critical role of dipeptidyl peptidase I in neutrophil recruitment during the development of experimental abdominal aortic aneurysms. Proc Natl Acad Sci U S A 104(8):2855-60. [PubMed: 17301245] [MGI Ref ID J:125910]
Park DJ; Agarwal A; George JF. 2010. Heme oxygenase-1 expression in murine dendritic cell subpopulations: effect on CD8+ dendritic cell differentiation in vivo. Am J Pathol 176(6):2831-9. [PubMed: 20395442] [MGI Ref ID J:161159]
Phan TG; Green JA; Gray EE; Xu Y; Cyster JG. 2009. Immune complex relay by subcapsular sinus macrophages and noncognate B cells drives antibody affinity maturation. Nat Immunol 10(7):786-93. [PubMed: 19503106] [MGI Ref ID J:150134]
Pi L; Xia H; Liu J; Shenoy AK; Hauswirth WW; Scott EW. 2011. Role of connective tissue growth factor in the retinal vasculature during development and ischemia. Invest Ophthalmol Vis Sci 52(12):8701-10. [PubMed: 21969300] [MGI Ref ID J:189463]
Reboldi A; Coisne C; Baumjohann D; Benvenuto F; Bottinelli D; Lira S; Uccelli A; Lanzavecchia A; Engelhardt B; Sallusto F. 2009. C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the choroid plexus is required for the initiation of EAE. Nat Immunol 10(5):514-23. [PubMed: 19305396] [MGI Ref ID J:148286]
Robbins CS; Chudnovskiy A; Rauch PJ; Figueiredo JL; Iwamoto Y; Gorbatov R; Etzrodt M; Weber GF; Ueno T; van Rooijen N; Mulligan-Kehoe MJ; Libby P; Nahrendorf M; Pittet MJ; Weissleder R; Swirski FK. 2012. Extramedullary hematopoiesis generates Ly-6C(high) monocytes that infiltrate atherosclerotic lesions. Circulation 125(2):364-74. [PubMed: 22144566] [MGI Ref ID J:193795]
Rodeheffer MS; Birsoy K; Friedman JM. 2008. Identification of white adipocyte progenitor cells in vivo. Cell 135(2):240-9. [PubMed: 18835024] [MGI Ref ID J:147623]
Rork TH; Wallace KL; Kennedy DP; Marshall MA; Lankford AR; Linden J. 2008. Adenosine A2A receptor activation reduces infarct size in the isolated, perfused mouse heart by inhibiting resident cardiac mast cell degranulation. Am J Physiol Heart Circ Physiol 295(5):H1825-33. [PubMed: 18757481] [MGI Ref ID J:142451]
Ruckh JM; Zhao JW; Shadrach JL; van Wijngaarden P; Rao TN; Wagers AJ; Franklin RJ. 2012. Rejuvenation of regeneration in the aging central nervous system. Cell Stem Cell 10(1):96-103. [PubMed: 22226359] [MGI Ref ID J:181499]
Ryan CM; Schell TD. 2006. Accumulation of CD8+ T cells in advanced-stage tumors and delay of disease progression following secondary immunization against an immunorecessive epitope. J Immunol 177(1):255-67. [PubMed: 16785521] [MGI Ref ID J:114942]
Schaeffer M; Han SJ; Chtanova T; van Dooren GG; Herzmark P; Chen Y; Roysam B; Striepen B; Robey EA. 2009. Dynamic imaging of T cell-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii. J Immunol 182(10):6379-93. [PubMed: 19414791] [MGI Ref ID J:148321]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Mating System Homozygote x Homozygote (Female x Male) 09-FEB-10 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Homozygous for Tg(UBC-GFP)30Scha
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Tg(UBC-GFP)30Scha x Homozygous for Tg(UBC-GFP)30Scha Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Homozygous for Tg(UBC-GFP)30Scha
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Tg(UBC-GFP)30Scha x Homozygous for Tg(UBC-GFP)30Scha Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 001026 BALB/cByJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.