Strain Name: |
CByJ.B6-Tg(UBC-GFP)30Scha/J |
|---|---|
Stock Number: |
007076 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | GFP; UBC; Tg(UBC-GFP)30Scha; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation N6+_ (22-JAN-08) Strain Description
These transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the direction of the human ubiqutin C promoter. Mice hemizygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice express EGFP in all tissues examined. Certain hematopoetic cell types display distinct expression levels of EGFP, allowing identification of different cells types by FACS analysis. EGFP expression is uniform within a cell type lineage and remains constant throughout development. T cells have a 2-fold higher EGFP expression than CD19+B220+ B cells or peripheral blood cells. Leukocytes and red blood cells from homozygous mice fluoresce at approximately twice the level of cells from hemizygous mice. This mutant mouse strain represents a useful tool in studies related to hematopoetic cell differentiation and in vivo tracking of leukocytes.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A transgenic construct containing an enhanced green fluorescent protein (EGFP) open reading frame under the control of the human ubiqutin C promoter was microinjected into fertilized C57BL/6 oocytes. Mice were screened for genomic integration of the transgene by PCR and for GFP expression in peripheral blood leukocytes. The resulting female mouse exhibiting the desired phenotype was bred to a male C57BL/6J mouse. F1 progeny from this cross that expressed GFP were intercrossed to produce homozygotes. The mice were then backcrossed to BALB/cBy for 5 generations before arriving at The Jackson Laboratory.
Gene & Allele Details
| Allele Symbol | Tg(UBC-GFP)30Scha | ||
|---|---|---|---|
| Allele Name | transgene insertion 30, Brian Schaefer | ||
| Common Name(s) | UBC-GFP; UBI-GFP; | ||
| Mutation Made By | Brian Schaefer, Uniformed Services Univ. Health Sciences | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | Certain hematopoietic cell types display distinct expression levels of GFP. Expression is uniform within a cell type lineage and remains constant throughout development. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | UBC, ubiquitin C, human | ||
| General Note | Homozygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These mice express GFP in all tissues examined. Expression levels vary between certain hematapoetic cell types. GFP expression is uniform within a cell type lineage and remains constant throughout development. T cells have a 2-fold higher GFP expression than CD19+B220+ B cells or peripheral blood cells. Leukocytes and red blood cells from homozygous transgenic mice fluoresce at approximately twice the level of cells from hemizygous mice. | ||
| Molecular Note | The transgene contains an enhanced green fluorescent protein open reading frame under the control of the human ubiquitin C promoter. [J:93490] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001026 BALB/cByJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Fluorescent Proteins (Generic GFP)
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as hemizygotes. The Donating Investigator has not attempted to make this strain homozygous on the BALB/cByJ background. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (138 strains)
004353 C57BL/6-Tg(UBC-GFP)30Scha/J
006259 B6.Cg-Pepcb Ptprca Tg(UBC-scFv)2Nemz/J 007001 B6;129S-Tg(UBC-cre/ESR1)1Ejb/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedHematological Research
Immunology and Inflammation Research
Research Tools
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers)
Hematological Research
Immunology and Inflammation Research
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesSchaefer BC; Schaefer ML; Kappler JW; Marrack P; Kedl RM. 2001. Observation of antigen-dependent CD8+ T-cell/ dendritic cell interactions in vivo. Cell Immunol 214(2):110-22. [PubMed: 12088410] [J:93490]
Price and Supply Information
| Strain Name: | CByJ.B6-Tg(UBC-GFP)30Scha/J |
| Stock Number: | 007076 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $236.40 | Hemizygous for Tg(UBC-GFP)30Scha | |||
| Pair | $288.65 | Hemizygous for Tg(UBC-GFP)30Scha x Noncarrier | |||
| Pair | $288.65 | Noncarrier x Hemizygous for Tg(UBC-GFP)30Scha | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE only available to non-profit entities.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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