Strain Name:

B6.129S4-Soat1tm1Far/Pgn

Stock Number:

007147

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Availability:

Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain 129S4/SvJae
H2 Haplotypeb
GenerationN6pN1
Generation Definitions

Description
Phenotypically similar to B6.129S4-Soat1tm1Far/J (003322), B6.129S4-Soat1tm1Far/Pgn has a reduced congenic interval making the closely linked Apoa2 gene of C57BL/6 rather than 129S4 origin. The donating investigator indicates that HDL levels exhibit a 24% increase on high fat diet in this strain in contrast to the 73% increase observed in the original strain. Triglyceride levels are unchanged. This strain may be useful in atherosclerosis research.

Development
This strain is derived from B6.129S4-Soat1tm1Far/J (003322) by further backcrossing to C57BL/6 reducing the congenic interval to a region defined by rs4222719 and D1Mit159. The reduced interval excludes the linked gene Apoa2 from the 129S4 derived region. Dr. Beverly Paigen performed the additional backcrosses at The Jackson Laboratory; the strain was cryopreserved in 2007.

Related Strains

Strains carrying   Soat1tm1Far allele
003322   B6.129S4-Soat1tm1Far/J
002896   B6;129S4-Soat1tm1Far/J
View Strains carrying   Soat1tm1Far     (2 strains)

Strains carrying other alleles of Soat1
000648   AKR/J
View Strains carrying other alleles of Soat1     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Soat1tm1Far/Soat1tm1Far

        involves: 129S4/SvJae * C57BL/6
  • endocrine/exocrine gland phenotype
  • abnormal adrenal gland morphology
    • adrenal glands are translucent and brownish instead of opaque and whitish-yellow as in wild-type and lack neutral lipids   (MGI Ref ID J:37049)
  • homeostasis/metabolism phenotype
  • abnormal cholesterol homeostasis
    • decrease in cholesterol esterification in fibroblasts and adrenal membranes   (MGI Ref ID J:37049)
    • esterification activity in fibroblasts is not stimulated in response to cholesterol (human LDL or 25-hydroxycholesterol)   (MGI Ref ID J:37049)
    • hepatic cholesterol ester levels are 30% lower in mutants fed a chow diet but are similar to wild-type when fed a high-fat diet   (MGI Ref ID J:37049)
    • increased circulating cholesterol level
      • on a chow or high-fat diet, mutants have higher plasma levels of total and HDL cholesterol than wild-type   (MGI Ref ID J:37049)
      • increased circulating HDL cholesterol level   (MGI Ref ID J:37049)
  • abnormal lipid level
    • reduction in cholesterol ester levels in adrenal glands and in peritoneal macrophages   (MGI Ref ID J:37049)
    • increased circulating cholesterol level
      • on a chow or high-fat diet, mutants have higher plasma levels of total and HDL cholesterol than wild-type   (MGI Ref ID J:37049)
      • increased circulating HDL cholesterol level   (MGI Ref ID J:37049)
  • hematopoietic system phenotype
  • abnormal macrophage morphology
    • reduction in cholesterol ester levels in peritoneal macrophages   (MGI Ref ID J:37049)
  • immune system phenotype
  • abnormal macrophage morphology
    • reduction in cholesterol ester levels in peritoneal macrophages   (MGI Ref ID J:37049)
  • liver/biliary system phenotype
  • abnormal liver morphology
    • hepatic cholesterol ester levels are 30% lower in mutants fed a chow diet but are similar to wild-type when fed a high-fat diet   (MGI Ref ID J:37049)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Soat1tm1Far related

Cardiovascular Research
Atherosclerosis

Internal/Organ Research
Adrenal Cortex Defects

Metabolism Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Soat1tm1Far
Allele Name targeted mutation 1, Bob Farese
Allele Type Targeted (Null/Knockout)
Common Name(s) Acact-; Acat-1-;
Mutation Made By Robert Farese, Jr.,   Gladstone Institute UCSF
Strain of Origin129S4/SvJae
ES Cell Line NameRF8
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Soat1, sterol O-acyltransferase 1
Chromosome 1
Gene Common Name(s) 8430426K15Rik; ACACT; ACAT; ACAT-1; ACAT1; AW550831; Acact; RIKEN cDNA 8430426K15 gene; SOAT; STAT; acetyl coenzyme A cholesterol acyltransferase; adrenocortical lipid depletion; ald; expressed sequence AW550831; hair interior defect; hid;
Molecular Note Insertion of a neomycin cassette into a 5' early exon (amino acids 50-100). Reduced levels of a truncated mRNA transcript were detected by Northern blot on RNA from preputial glands. No protein was detected by immunoblot analysis in homogenates from preputial glands, ovaries and adrenals. [MGI Ref ID J:37049]

Genotyping

Genotyping Information

Genotyping Protocols

Soat1tm1Far-Alternate 1, Standard PCR
Soat tm1Far, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Meiner VL; Cases S; Myers HM; Sande ER; Bellosta S; Schambelan M; Pitas RE; McGuire J; Herz J; Farese RV Jr. 1996. Disruption of the acyl-CoA:cholesterol acyltransferase gene in mice: evidence suggesting multiple cholesterol esterification enzymes in mammals. Proc Natl Acad Sci U S A 93(24):14041-6. [PubMed: 8943057]  [MGI Ref ID J:37049]

Additional References

Soat1tm1Far related

Accad M; Smith SJ; Newland DL; Sanan DA; King LE Jr; Linton MF; Fazio S; Farese RV Jr. 2000. Massive xanthomatosis and altered composition of atherosclerotic lesions in hyperlipidemic mice lacking acyl CoA:cholesterol acyltransferase 1 [see comments] J Clin Invest 105(6):711-9. [PubMed: 10727439]  [MGI Ref ID J:61147]

Bryleva EY; Rogers MA; Chang CC; Buen F; Harris BT; Rousselet E; Seidah NG; Oddo S; LaFerla FM; Spencer TA; Hickey WF; Chang TY. 2010. ACAT1 gene ablation increases 24(S)-hydroxycholesterol content in the brain and ameliorates amyloid pathology in mice with AD. Proc Natl Acad Sci U S A 107(7):3081-6. [PubMed: 20133765]  [MGI Ref ID J:157545]

Cui D; Thorp E; Li Y; Wang N; Yvan-Charvet L; Tall AR; Tabas I. 2007. Pivotal advance: macrophages become resistant to cholesterol-induced death after phagocytosis of apoptotic cells. J Leukoc Biol 82(5):1040-50. [PubMed: 17576822]  [MGI Ref ID J:127198]

Freeman NE; Rusinol AE; Linton M; Hachey DL; Fazio S; Sinensky MS; Thewke D. 2005. Acyl-coenzyme A:cholesterol acyltransferase promotes oxidized LDL/oxysterol-induced apoptosis in macrophages. J Lipid Res 46(9):1933-43. [PubMed: 15995174]  [MGI Ref ID J:100481]

Matynia A; Anagnostaras SG; Wiltgen BJ; Lacuesta M; Fanselow MS; Silva AJ. 2008. A high through-put reverse genetic screen identifies two genes involved in remote memory in mice. PLoS ONE 3(5):e2121. [PubMed: 18464936]  [MGI Ref ID J:136217]

Rodriguez A; Ashen MD; Chen ES. 2005. ACAT1 deletion in murine macrophages associated with cytotoxicity and decreased expression of collagen type 3A1. Biochem Biophys Res Commun 331(1):61-8. [PubMed: 15845358]  [MGI Ref ID J:97572]

Rogers MA; Liu J; Kushnir MM; Bryleva E; Rockwood AL; Meikle AW; Shapiro D; Vaisman BL; Remaley AT; Chang CC; Chang TY. 2012. Cellular pregnenolone esterification by acyl-CoA:cholesterol acyltransferase. J Biol Chem 287(21):17483-92. [PubMed: 22474282]  [MGI Ref ID J:185622]

Su YR; Dove DE; Major AS; Hasty AH; Boone B; Linton MF; Fazio S. 2005. Reduced ABCA1-mediated cholesterol efflux and accelerated atherosclerosis in apolipoprotein E-deficient mice lacking macrophage-derived ACAT1. Circulation 111(18):2373-81. [PubMed: 15851589]  [MGI Ref ID J:111648]

Su Z; Wang X; Tsaih SW; Zhang A; Cox A; Sheehan S; Paigen B. 2009. Genetic basis of HDL variation in 129/SvImJ and C57BL/6J mice: importance of testing candidate genes in targeted mutant mice. J Lipid Res 50(1):116-25. [PubMed: 18772481]  [MGI Ref ID J:144089]

Xie C; Zhou ZS; Li N; Bian Y; Wang YJ; Wang LJ; Li BL; Song BL. 2012. Ezetimibe blocks the internalization of NPC1L1 and cholesterol in mouse small intestine. J Lipid Res 53(10):2092-2101. [PubMed: 22811412]  [MGI Ref ID J:188044]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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