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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N?pN1
Generation DefinitionsDonating Investigator Dr. Arthur Weiss, University of California, San Francisco Description
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Homozygotes have increased expression of T cell receptors (TCR) on CD4+ CD8+ thymocytes, increased CD5 expression, and altered thymocyte development. B cell development is also altered. Northern blot and RT-PCR analyses confirm that the gene has been functionally inactivated.Development
A targeting vector was designed to replace a portion of the promoter region encoding amino acids 1-20 (470 bp) with a neomycin resistance gene. The vector was introduced to 129-derived embryonic stem (ES) cells. Positive clones were injected into C57BL/6 blastocysts and chimeric animals were crossed with C57BL/6. This line was backcrossed to C57BL/6 nine times by the donating laboratory.
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms provided by MGI
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Slatm1Weis/Slatm1Weis
involves: 129 * C57BL/6
- hematopoietic system phenotype
- abnormal double-positive T cell morphology
- immune system phenotype
- abnormal double-positive T cell morphology
- cellular phenotype
- abnormal double-positive T cell morphology
This mouse can be used to support research in many areas including:
Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Slatm1Weis | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Arthur Weiss | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | SLAP-; | ||
| Mutation Made By | Dr. Arthur Weiss, University of California, San Francisco | ||
| Strain of Origin | 129 | ||
| Gene Symbol and Name | Sla, src-like adaptor | ||
| Chromosome | 15 | ||
| Gene Common Name(s) | SLA1; SLAP; Slap-1; Slap1; | ||
| Molecular Note | The gene was disrupted by replacement of part of the promoter region and sequences encoding the first 21 amino acids with a neomycin resistance cassette. Northern blot using a probe to the C-terminal region of the gene and RT-PCR analysis of homozygous animals confirmed inactivation of the gene. Sequence analysis of a weak RT-PCR product revealed an alternatively spliced truncated transcript. [MGI Ref ID J:71804] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Slatm1Weis, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Sosinowski T; Killeen N; Weiss A. 2001. The Src-like adaptor protein downregulates the T cell receptor on CD4+CD8+ thymocytes and regulates positive selection. Immunity 15(3):457-66. [PubMed: 11567635] [MGI Ref ID J:71804]
Additional References
Slatm1Weis relatedDragone LL; Myers MD; White C; Gadwal S; Sosinowski T; Gu H; Weiss A. 2006. Src-like adaptor protein (SLAP) regulates B cell receptor levels in a c-Cbl-dependent manner. Proc Natl Acad Sci U S A 103(48):18202-7. [PubMed: 17110436] [MGI Ref ID J:117147]
Dragone LL; Myers MD; White C; Sosinowski T; Weiss A. 2006. SRC-like adaptor protein regulates B cell development and function. J Immunol 176(1):335-45. [PubMed: 16365426] [MGI Ref ID J:126262]
Hippe A; Bylaite M; Chen M; von Mikecz A; Wolf R; Ruzicka T; Walz M. 2006. Expression and tissue distribution of mouse Hax1. Gene 379:116-26. [PubMed: 16814492] [MGI Ref ID J:112369]
Liontos LM; Dissanayake D; Ohashi PS; Weiss A; Dragone LL; McGlade CJ. 2011. The Src-like adaptor protein regulates GM-CSFR signaling and monocytic dendritic cell maturation. J Immunol 186(4):1923-33. [PubMed: 21220694] [MGI Ref ID J:169170]
Mingueneau M; Sansoni A; Gregoire C; Roncagalli R; Aguado E; Weiss A; Malissen M; Malissen B. 2008. The proline-rich sequence of CD3epsilon controls T cell antigen receptor expression on and signaling potency in preselection CD4+CD8+ thymocytes. Nat Immunol 9(5):522-32. [PubMed: 18408722] [MGI Ref ID J:134506]
Myers MD; Dragone LL; Weiss A. 2005. Src-like adaptor protein down-regulates T cell receptor (TCR)-CD3 expression by targeting TCRzeta for degradation. J Cell Biol 170(2):285-94. [PubMed: 16027224] [MGI Ref ID J:100141]
Myers MD; Sosinowski T; Dragone LL; White C; Band H; Gu H; Weiss A. 2006. Src-like adaptor protein regulates TCR expression on thymocytes by linking the ubiquitin ligase c-Cbl to the TCR complex. Nat Immunol 7(1):57-66. [PubMed: 16327786] [MGI Ref ID J:112388]
Peterson LK; Shaw LA; Joetham A; Sakaguchi S; Gelfand EW; Dragone LL. 2011. SLAP deficiency enhances number and function of regulatory T cells preventing chronic autoimmune arthritis in SKG mice. J Immunol 186(4):2273-81. [PubMed: 21248251] [MGI Ref ID J:169146]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as heterozygotes or homozygotes.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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For Licensing and Use Restrictions view the link(s) below:
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Contact information
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| phone: | 207-288-6470 |
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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