Strain Name: |
B6.129X1-Notch1tm2Rko/GridJ |
|---|---|
Stock Number: |
007181 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Notch1; Notch1tm2Rko; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Raphael Kopan, Washington Univeristy Generation N5 (01-MAY-08) Strain Description
Mice homozygous for this "floxed" Notch1 allele (fN1) are viable and fertile. These mice possess loxP sites on either side of exon 1 of the targeted gene. When bred to mice with a Cre recombinase gene, exon 1 of the targeted gene is deleted in the cre expressing tissue(s). These conditional knockout mice may be useful in generating tissue-specific mutants for studying the development of a wide range of tissues: for example, when crossed to a strain expressing Cre recombinase primarily in the nervous system (see Stock No. 003771), this mutant strain may be useful in studies of apoptosis in neural development.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A targeting construct was designed to place a loxP-flanked PGK-neo cassette upstream of exon 1 of the targeted gene, as well as a single loxP site in intron 1. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre recombinase vector to remove the selection cassette. The resulting ES cells harboring the loxP-flanked exon 1 were injected into blastocysts to generate chimeric mice. Mutant mice were then sent to Dr. Thomas Gridley (The Jackson Laboratory) in 2003 on an unspecified mixed genetic background. These mice were supplied to The Jackson Laboratory Repository (as Stock No. 006951) in 2007. After this, some mice were backcrossed to C57BL/6J for at least 5 generations to generate this congenic strain (Stock No. 007181).
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Notch1tm2Rko | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Raphael Kopan | ||
| Common Name(s) | Notch1flox; Notch1tm1Shn; fN1; | ||
| Mutation Made By | Raphael Kopan, Washington Univeristy | ||
| Strain of Origin | 129X1/SvJ | ||
| ES Cell Line Name | RW-4 | ||
| ES Cell Line Strain | 129X1/SvJ | ||
| Gene Symbol and Name | Notch1, Notch gene homolog 1 (Drosophila) | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | 9930111A19Rik; Mis6; NOTCH; RIKEN cDNA 9930111A19 gene; TAN1; Tan1; hN1; lin-12; translocation-associated Notch; | ||
| Molecular Note | LoxP were inserted flanking the first coding exon of the gene. An adjacent loxP flanked neomycin cassette was removed by Cre-mediated recombination in ES cells prior to production of chimeric animals. [MGI Ref ID J:90392] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | Mutant mice were bred to C57BL/6J mice to generate this congenic strain. When maintaining the live congenic colony, mice may be bred as homozygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Notch1tm2Rko allele
006951 STOCK Notch1tm2Rko/GridJ View Strains carrying Notch1tm2Rko (1 strain)
002797 B6.129-Notch1tm1Con/J 002445 STOCK Notch1tm1Con/J
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Notch1 relatedCell Biology Research
Genes Regulating Growth and Proliferation
Signal Transduction
Developmental Biology Research
Growth Defects
Research Tools
Cell Biology Research
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)
Developmental Biology Research
Embryonic Lethality (Homozygous)
References
Selected Reference(s)
Additional ReferencesYang X; Klein R; Tian X; Cheng HT; Kopan R; Shen J. 2004. Notch activation induces apoptosis in neural progenitor cells through a p53-dependent pathway. Dev Biol 269(1):81-94. [PubMed: 15081359] [MGI Ref ID J:90392]
Price and Supply Information
| Strain Name: | B6.129X1-Notch1tm2Rko/GridJ |
| Stock Number: | 007181 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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