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Strain Name:

129S-Sgpl1Gt(ROSA)78Sor/J

Stock Number:

007199

Availability:

Repository- Live

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Genes & Alleles   Sgpl1;   Sgpl1Gt(ROSA)78Sor;

Product Information

Strain Details

Type JAX® GEMM® Strain - Gene Trap
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Stock
Type JAX® GEMM® Strain - Targeted Mutation
Mating System+/+ sibling x Heterozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Philippe Soriano,   Mount Sinai School of Medicine
Generation?+F1 (27-JUL-08)

Strain Description
Mice homozygous for this mutant allele have reduced size and weight gains after birth and do not survive past 8 weeks of age. Homozygotes occur at a lower than Mendelian ratio (19%) from heterozygote X heterozygote crosses. No gene product is detected in homozygous embryos aged ED9.5-12.5 or in adult gonad. Beta-galactosidase staining pattern mimics the endogenous gene expression pattern in adult intestinal epithelial cells. Homozygous embryos E11.5 to E18.5 exhibit hemorrhages and microaneurisms. Vascular defects persist into adulthood. At 6 weeks of age, mutant mice are anemic (low hemoglobin concentration, reduced red blood cell count, low hematocrit). Mutants exhibit polychromasia (abnormally high number of immature blood cells), kidney defects (blood urea nitrogen level abnormally high, kidney size smaller than wildtype, swollen blood filled glomeruli, reduced number of vascular smooth muscle cells) and abnormalities in palate bone fusion. Homozygotes are infertile. Heterozygotes are viable and fertile. These Sgpl1-mutant mice may be useful in studying cellular signaling in development and adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family) and immediate early genes (IEG) induced shortly after RTK activation.

Strain Development
The retroviral gene-trap vector ROSAFARY (or reverse orientation splice acceptor for array) was designed with a promoter trap module (SAbgeo*pA; encoding a lacZ-neo fusion gene) and an frt-flanked poly-A trap module (PGKhygSD; PGK promoter-driven hygromycin gene with adenoviral splice donor). This construct was electroporated into 129S4/SvJaeSor-derived AK7.1 embryonic stem (ES) cells. ES cells with the ROSAFARY vector correctly targeted to the Sgpl1 gene (in the 2nd intron) were identified and used to generate mutant mice. Homozygous mice were maintained on a 129S4/SvJaeSor background prior to arrival at The Jackson Laboratory. The mice were then crossed to 129S1/SvImJ (Stock No. 002448) for one generation.

The ROSAFARY vector inserts a promoterless lacZ-neo reporter fusion gene (which functions as an artificial 3' terminal exon to intercept and terminate transcription from the targeted gene promoter) as well as a poly-A trap (which functions as an artificial 5' terminal exon to initiate transcription from the insertion site). After inserting into an intron of an endogenous gene at a permissive site and in the correct orientation, the promoter trap module and the poly-A trap module can be activated to form fusion transcripts with the 5' or 3' exons.

Mammalian Phenotype Terms assigned by genotype

Sgpl1Gt(ROSA)78Sor/Sgpl1Gt(ROSA)78Sor

        involves: 129S4/SvJaeSor
  • life span-post-weaning/aging
  • premature death (MGI Ref ID J:117491)
    • all mice die by 8 weeks
  • skeleton phenotype
  • abnormal skeleton morphology (MGI Ref ID J:117491)
    • abnormal neural crest derived and thoracic skeleton
    • abnormal calvaria morphology (MGI Ref ID J:117491)
      • 75% of mice have reduced calvarial bones at the midline with increases in the gaps between frontal bones
      • abnormal frontal bone morphology (MGI Ref ID J:117491)
        • increases in the gaps between frontal bones
    • abnormal palatine shelf (MGI Ref ID J:117491)
      • 75% of mice have smaller and less extended towards the midline palatine and presphenoid bones
    • abnormal presphenoid bone morphology (MGI Ref ID J:117491)
      • 75% of mice have smaller and less extended towards the midline palatine and presphenoid bones
    • abnormal sternum morphology (MGI Ref ID J:117491)
      • 50% of mice have sternum defects including asymmetric or additional fusion of ribs and gaps in the sternum
      • asymmetric rib-sternum attachment (MGI Ref ID J:117491)
    • abnormal vertebrae morphology (MGI Ref ID J:117491)
      • 20% of mice have widened vertebral arches and forked vertebrae
      • abnormal vertebral arch morphology (MGI Ref ID J:117491)
        • 20% of mice have widened vertebral arches and forked vertebrae
  • renal/urinary system phenotype
  • abnormal kidney morphology (MGI Ref ID J:117491)
    • abnormal renal glomerulus morphology (MGI Ref ID J:117491)
      • glomeruli are often degraded
      • smooth muscle cell number is reduced in the glomeruli
      • migration of smooth muscle cells in glomeruli is impaired
    • pale kidney (MGI Ref ID J:117491)
    • small kidney (MGI Ref ID J:117491)
  • abnormal kidney physiology (MGI Ref ID J:117491)
    • decrease in kidney function
    • kidney inflammation (MGI Ref ID J:117491)
      • kidney is swollen in sections and blood filled
  • hematopoietic system phenotype
  • abnormal red blood cell (MGI Ref ID J:117491)
    • an increase in immature red blood cells is observed compared to wild type mice
    • anemia (MGI Ref ID J:117491)
      • by week 6
    • decreased erythrocyte cell number (MGI Ref ID J:117491)
      • by week 6
    • decreased hematocrit (MGI Ref ID J:117491)
      • by week 6
    • decreased hemoglobin content (MGI Ref ID J:117491)
      • by week 6
    • polychromatophilia (MGI Ref ID J:117491)
  • growth/size phenotype
  • decreased body weight (MGI Ref ID J:117491)
  • postnatal growth retardation (MGI Ref ID J:117491)
  • cellular phenotype
  • abnormal cell migration (MGI Ref ID J:117491)
    • migration of smooth muscle cells in glomeruli is impaired
    • mouse embryonic fibroblast cells show reduced migration in a scratch test in response to PDGF stimulation
  • homeostasis/metabolism phenotype
  • increased blood urea nitrogen level (MGI Ref ID J:117491)
  • cardiovascular system phenotype
  • abnormal vasculature (MGI Ref ID J:117491)
  • hemorrhage (MGI Ref ID J:117491)
    • at E11.5-E18.5
  • muscle phenotype
  • abnormal smooth muscle morphology (MGI Ref ID J:117491)
    • smooth muscle cell number is reduced in the glomeruli
  • immune system phenotype
  • kidney inflammation (MGI Ref ID J:117491)
    • kidney is swollen in sections and blood filled
  • craniofacial phenotype
  • abnormal calvaria morphology (MGI Ref ID J:117491)
    • 75% of mice have reduced calvarial bones at the midline with increases in the gaps between frontal bones
    • abnormal frontal bone morphology (MGI Ref ID J:117491)
      • increases in the gaps between frontal bones
  • abnormal palate morphology (MGI Ref ID J:117491)
    • irregularities in fusion persist into adulthood
    • abnormal palatine shelf (MGI Ref ID J:117491)
      • 75% of mice have smaller and less extended towards the midline palatine and presphenoid bones
  • abnormal presphenoid bone morphology (MGI Ref ID J:117491)
    • 75% of mice have smaller and less extended towards the midline palatine and presphenoid bones
  • digestive/alimentary phenotype
  • abnormal palate morphology (MGI Ref ID J:117491)
    • irregularities in fusion persist into adulthood
    • abnormal palatine shelf (MGI Ref ID J:117491)
      • 75% of mice have smaller and less extended towards the midline palatine and presphenoid bones

Gene & Allele Details

Allele Symbol Sgpl1Gt(ROSA)78Sor
Allele Name gene trapped 78, Philippe M Soriano
Common Name(s) Sgpl1-;
Mutation Made By Philippe Soriano,   Fred Hutchinson Cancer Research Center
Strain of Origin129S4/SvJaeSor
ES Cell Line NameAK7
ES Cell Line Strain129S4/SvJaeSor
Site of ExpressionlacZ expression pattern mimics the endogenous gene expression pattern in adult intestinal epithelial cells.
Gene Symbol and Name Sgpl1, sphingosine phosphate lyase 1
Chromosome 10
Gene Common Name(s) AI428538; D10Xrf456; FLJ13811; KIAA1252; SPL; Spgl1; expressed sequence AI428538;
Molecular Note The Rosafary gene trap vector containing a SAbetaGeo pA promoter trap module and a PGKhygSD poly-A trap module was inserted into intron 2. [MGI Ref ID J:117491]

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for JAX® GEMM® Strains

Genotyping Protocols

Sgpl1Gt(ROSA)78Sor

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as heterozygotes. Homozygotes are not fertile and die by 8 weeks of age.
Diet Information LabDiet® 5K52/5K67

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005771   B6.129P2-Adamts5tm1Dgen/J
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005777   B6.129P2-Axltm1Dgen/J
005783   B6.129P2-Cacna1ctm1Dgen/J
005780   B6.129P2-Cacna2d3tm1Dgen/J
005781   B6.129P2-Cacng3tm1Dgen/J
005782   B6.129P2-Cacng4tm1Dgen/J
005784   B6.129P2-Capn5tm1Dgen/J
005785   B6.129P2-Capn7tm1Dgen/J
005792   B6.129P2-Ccr1l1tm1Dgen/J
005793   B6.129P2-Ccr6tm1Dgen/J
005794   B6.129P2-Ccr7tm1Dgen/J
005779   B6.129P2-Celsr2tm1Dgen/J
005797   B6.129P2-Chrna2tm1Dgen/J
005787   B6.129P2-Ctsctm1Dgen/J
005796   B6.129P2-Cxcr3tm1Dgen/J
005798   B6.129P2-Drd5tm1Dgen/J
005800   B6.129P2-Efemp2tm1Dgen/J
005801   B6.129P2-Esrratm1Dgen/J
005802   B6.129P2-Faim2tm1Dgen/J
005803   B6.129P2-Fzd1tm1Dgen/J
005804   B6.129P2-Fzd8tm1Dgen/J
005811   B6.129P2-Gabra3tm1Dgen/J
005812   B6.129P2-Gabra4tm1Dgen/J
005810   B6.129P2-Gabrptm1Dgen/J
005809   B6.129P2-Galr1tm1Dgen/J
005816   B6.129P2-Glra3tm1Dgen/J
005805   B6.129P2-Gpr151tm1Dgen/J
005806   B6.129P2-Gpr37tm1Dgen/J
005807   B6.129P2-Gpr6tm1Dgen/J
005813   B6.129P2-Grik5tm1Dgen/J
005808   B6.129P2-Grk5tm1Dgen/J
005814   B6.129P2-Grm1tm1Dgen/J
005815   B6.129P2-Grm3tm1Dgen/J
005817   B6.129P2-Gsk3btm1Dgen/J
005818   B6.129P2-Hcrtr1tm1Dgen/J
005767   B6.129P2-Htr4tm1Dgen/J
005769   B6.129P2-Htr7tm1Dgen/J
005830   B6.129P2-Kcnq2tm1Dgen/J
005821   B6.129P2-Lats2tm1Dgen/J
005822   B6.129P2-Lmbr1tm1Dgen/J
005850   B6.129P2-Mapkapk2tm1Dgen/J
005824   B6.129P2-Mmp17tm1Dgen/J
005825   B6.129P2-Mtmr1tm1Dgen/J
005778   B6.129P2-Naip1tm1Dgen/J
005826   B6.129P2-Ntsr1tm1Dgen/J
005829   B6.129P2-Pkd2l2tm1Dgen/J
005828   B6.129P2-Ppardtm1Dgen/J
005831   B6.129P2-Ppm1ftm1Dgen/J
005827   B6.129P2-Ptch2tm1Dgen/J
005832   B6.129P2-Ptprotm1Dgen/J
005799   B6.129P2-S1pr4tm1Dgen/J
005837   B6.129P2-Scn11atm1Dgen/J
005836   B6.129P2-Scn9atm1Dgen/J
005834   B6.129P2-Sema5atm1Dgen/J
005835   B6.129P2-Sema6ctm1Dgen/J
006432   B6.129P2-Slc18a1tm1Dgen/J
005839   B6.129P2-Slc22a12tm1Dgen/J
005838   B6.129P2-Slc22a6tm1Dgen/J
005840   B6.129P2-Slc40a1tm1Dgen/J
005841   B6.129P2-Slc6a9tm1Dgen/J
005842   B6.129P2-Slc7a8tm1Dgen/J
005843   B6.129P2-Slc9a6tm1Dgen/J
005844   B6.129P2-Sstr1tm1Dgen/J
005847   B6.129P2-Tgfbr1tm1Dgen/J
005845   B6.129P2-Thbs4tm1Dgen/J
005790   B6.129P2-Tpp1tm1Dgen/J
005848   B6.129P2-Trpm5tm1Dgen/J
005791   B6.129P2-Xcr1tm1Dgen/J
003474   B6.129S4-Gt(ROSA)26Sortm1Sor/J
005901   B6.129S4-Ppardtm2Rev/J
006142   B6.129S4-Ppargtm1Rev/J
003754   B6.129S4-Shroom3Gt(ROSA)53Sor/J
005119   B6.129S6-Npas2tm1Slm/J
002741   B6.129S7-Alpltm1Sor/J
005970   B6.129S7-Atoh1tm2Hzo/J
006039   B6.129S7-Efnb2tm1And/J
002192   B6.129S7-Gt(ROSA)26Sor/J
005981   B6.129S7-Rai1tm1Jrl/J
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006262   B6.129X1-Fut2tm1Sdo/J
005085   B6.Cg-Cd44tm1Hbg/J
007745   B6.Cg-Mirn155tm1.1Rsky/J
005317   B6.Cg-Tg(BAT-lacZ)3Picc/J
006055   B6.Cg-Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
006477   B6.Cg-Tg(CAG-lacZ-WGA)330Bbm/J
003139   B6.Cg-Tg(DBHn-lacZ)8Rpk/J
006229   B6.Cg-Tg(DRE-lacZ)2Gswz/J
002982   B6.Cg-Tg(xstpx-lacZ)32And/J
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005064   B6;129-Slc30a3tm1Rpa/J
005788   B6;129P2-Cd97tm1Dgen/J
005833   B6;129P2-Rgs4tm1Dgen/J
002073   B6;129S-Gt(ROSA)26Sor/J
006470   B6;129S-Hopxtm1Eno/J
004153   B6;129S-Mtap7Gt(ROSABetageo)1Sor/J
006958   B6;129S-Nkd1tm1Kwha/J
006960   B6;129S-Nkd2tm1Kwha/J
007204   B6;129S4-2610005L07RikGt(ROSA)73Sor/J
003309   B6;129S4-Gt(ROSA)26Sortm1Sor/J
004365   B6;129S6-Srebf1tm1Mbr/J
002317   B6;129S7-Alpltm1Sor/J
003266   B6;129S7-Epas1tm1Rus/J
006044   B6;129S7-Ephb4tm1And/J
003471   B6;C3H-Tg(CNP-GEO)1Ldh/J
006465   B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J
006680   B6;CBA-Tg(Olfr16*,taulacZ)19Mom/MomJ
006671   B6;CBA-Tg(Olfr16*,taulacZ)5Mom/MomJ
006672   B6;CBA-Tg(Olfr16*,taulacZ)7Mom/MomJ
006673   B6;CBA-Tg(Olfr16,taulacZ)sn2Mom/MomJ
004141   B6;CBA-Tg(UAS-lacZ)65Rth/J
002369   B6;SJL-Tg(c177-lacZ)226Bri/J
002372   B6;SJL-Tg(c177-lacZ)227Bri/J
002621   B6;SJL-Tg(tetop-lacZ)2Mam/J
003299   B6;SWJ-Tg(TIMP3-lacZ)7Jeb/J
002865   B6CBA-Tg(Wnt1-lacZ)206Amc/J
002955   C.129S7-Gt(ROSA)26Sor/J
002754   C57BL/6-Tg(LacZpl)60Vij/J
002193   C57BL/6J-Tg(MTn-lacZ)204Bri/J
002981   DBA/2-Tg(xstpx-lacZ)36And/J
004127   FVB-Tg(Nes-rtTA)306Rvs/J
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008203   FVB.Cg-Smn1tm1Msd Tg(ACTA1-SMN)63Ahmb Tg(SMN2)89Ahmb/J
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006214   FVB.Cg-Smn1tm1Msd/J
005024   FVB.Cg-Tg(SMN2)89Ahmb Smn1tm1Msd/J
005026   FVB.Cg-Tg(SMN2)89Ahmb Tg(SMN1*A2G)2023Ahmb Smn1tm1Msd/J
005025   FVB.Cg-Tg(SMN2*delta7)4299Ahmb Tg(SMN2)89Ahmb Smn1tm1Msd/J
003140   FVB/N-Tg(PAI1-lacZ)1Jjb/J
002856   FVB/N-Tg(TIE2-lacZ)182Sato/J
005941   FVB/N-Tg(tetO-Aurkb,lacZ)41Kra/J
003315   FVB/N-Tg(tetORo1-lacZ)3Conk/J
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005438   STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
006850   STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
006876   STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J
006613   STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J
003919   STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J
003920   STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
004623   STOCK Tg(Fos-lacZ)34Efu/J
006674   STOCK Tg(Olfr16,taulacZ)2030Mom/MomJ
005493   STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J
002395   STOCK Tg(Zfy1-lacZ)218Bri/J
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View lacZ Expression Strains     (174 strains)

Additional Web Information

Fluorescent Proteins/lacZ Systems

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Cardiovascular Research
Vascular Defects

Cell Biology Research
Signal Transduction

Developmental Biology Research
Craniofacial and Palate Defects
Embryonic Lethality (Homozygous)
Growth Defects
Internal/Organ Defects (kidney)
Internal/Organ Defects (multiple)
Internal/Organ Defects (vasculature)
Skeletal Defects

Hematological Research
Hematopoietic Defects

Internal/Organ Research
Kidney Defects

Research Tools
lacZ Expression
Cardiovascular Research
Cell Biology Research
Developmental Biology Research
Genetics Research (Tissue/Cell Markers)
Hematological Research
Internal/Organ Research

References

Selected Reference(s)

Schmahl J; Raymond CS; Soriano P. 2007. PDGF signaling specificity is mediated through multiple immediate early genes. Nat Genet 39(1):52-60. [PubMed: 17143286]  [MGI Ref ID J:117491]

Additional References

Price and Supply Information

Strain Name: 129S-Sgpl1Gt(ROSA)78Sor/J
Stock Number: 007199

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:

*Pricing for Shipping Destination selected:

        International

Price(s) in US dollars ($)Genotype(s) Provided
Individual Mouse Price $307.40Heterozygous for Sgpl1Gt(ROSA)78Sor
Pair $375.30Heterozygous for Sgpl1Gt(ROSA)78Sor x Wild-type for Sgpl1Gt(ROSA)78Sor
Pair $375.30Wild-type for Sgpl1Gt(ROSA)78Sor x Heterozygous for Sgpl1Gt(ROSA)78Sor

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845.
Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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