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Genes & Alleles

Plekha1;   Plekha1Gt(ROSA)82Sor;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Gene Trap Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Stock Mating System Heterozygote x +/+ sibling         (Female x Male) Species laboratory mouse Donating Investigator Philippe Soriano,   Fred Hutchinson Cancer Research Center Generation N?+F1 (08-JAN-08)

Strain Description
25% of homozygotes die by 2 weeks of age. Homozygous males are infertile and, after 3 weeks of age, exhibit higher than normal (wildtype) weight gain. No gene product is detected in homozygous embryos aged ED9.5-12.5 or in adult gonad. These Plekha1-mutant mice may be useful in studying cellular signaling in development and adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family) and immediate early genes (IEG) induced shortly after RTK activation.

Strain Development
The retroviral gene-trap vector ROSAFARY (or reverse orientation splice acceptor for array) was designed with a promoter trap module (SAbgeo*pA; encoding a lacZ-neo fusion gene) and an frt-flanked poly-A trap module (PGKhygSD; PGK promoter-driven hygromycin gene with adenoviral splice donor). This construct was electroporated into 129S4/SvJaeSor-derived AK7.1 embryonic stem (ES) cells. ES cells with the ROSAFARY vector correctly targeted to the Plekha1 gene (in the 3rd intron) were identified and used to generate mutant mice. Heterozygous mice were maintained on a mixed B6;129 background prior to arrival at The Jackson Laboratory. The mice were then crossed to C57BL/6J (Stock No. 000664) for one generation.

The ROSAFARY vector inserts a promoterless lacZ-neo reporter fusion gene (which functions as an artificial 3' terminal exon to intercept and terminate transcription from the targeted gene promoter) as well as a poly-A trap (which functions as an artificial 5' terminal exon to initiate transcription from the insertion site). After inserting into an intron of an endogenous gene at a permissive site and in the correct orientation, the promoter trap module and the poly-A trap module can be activated to form fusion transcripts with the 5' or 3' exons.

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Plekha1Gt(ROSA)82Sor/Plekha1Gt(ROSA)82Sor         involves: 129S4/SvJaeSor lethality-postnatal postnatal lethality (MGI Ref ID J:117491) 25% of mice die by week 2 growth/size phenotype increased body weight (MGI Ref ID J:117491) in males following adolescence

Gene & Allele Details

Allele Symbol		Plekha1Gt(ROSA)82Sor
Allele Name		gene trapped 82, Philippe M Soriano
Common Name(s)		Plekha1-;
Mutation Made By		Philippe Soriano,   Fred Hutchinson Cancer Research Center
Strain of Origin		129S4/SvJaeSor
ES Cell Line Name		AK7
ES Cell Line Strain		129S4/SvJaeSor
Gene Symbol and Name		Plekha1, pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
Chromosome		7
Gene Common Name(s)		AA960558; C920009D07Rik; MGC156706; RGD1564153; RIKEN cDNA C920009D07 gene; TAPP1; expressed sequence AA960558;
Molecular Note		The Rosafary gene trap vector containing a SAbetaGeo pA promoter trap module and a PGKhygSD poly-A trap module was inserted into intron 3. [MGI Ref ID J:117491]

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls

Genotyping Protocols

Plekha1^{Gt(ROSA)82Sor}

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as heterozygotes. 25% of homozygotes die by 2 weeks of age. Homozygote males are infertile. The Donating Investigator used a heterozygous male x homozygous female breeding scheme.

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Signal Transduction

Developmental Biology Research
Growth Defects Growth Defects (homozygous)
Perinatal Lethality (Homozygous)

Reproductive Biology Research
Fertility Defects (males only)

References

Selected Reference(s)

Schmahl J; Raymond CS; Soriano P. 2007. PDGF signaling specificity is mediated through multiple immediate early genes. Nat Genet 39(1):52-60. [PubMed: 17143286]  [MGI Ref ID J:117491]

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Price and Supply Information

Strain Name:	B6;129S4-Plekha1Gt(ROSA)82Sor/J
Stock Number:	007201

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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