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| These BC058969-mutant (2610005L07Rik-mutant) mice may be useful in studying cellular signaling in development and adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family) and immediate early genes (IEG) induced shortly after RTK activation. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6;129S4-Cdh11Gt(ROSA)73Sor/J (Changed: 04-DEC-07 ) Type Gene Trap; Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Heterozygote x +/+ sibling (Female x Male) Species laboratory mouse Generation ?+F1 (03-DEC-07) Donating Investigator Philippe Soriano, Fred Hutchinson Cancer Research Center Description
Mice homozygous for this mutant allele (called BC058969 in the primary publication) are viable and fertile, with greater than 50% embryonic lethality observed in homozygous embryos. Homozygotes occur at a lower than Mendelian ratio (9%) from heterozygote x heterozygote crosses. No gene product is detected in homozygous embryos aged ED9.5-12.5 or in adult gonad. Homozygotes exhibit defects that affect the same cell types and processes as those controlled by the platelet-derived growth factor (PDGF) pathway, including vasculature, kidney, and skeletal defects (sternum and calvarial bones). Notably, 100% incidence of calvarial bones defects is reported. Additionally, homozygotes are reported to have low β-galactosidase activity; in situ hybridization or other sensitive methods may be necessary to detect expression of the lacZ-neo reporter fusion gene. These BC058969-mutant (2610005L07Rik-mutant) mice may be useful in studying cellular signaling in development and adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family) and immediate early genes (IEG) induced shortly after RTK activation.Development
The retroviral gene-trap vector ROSAFARY (or reverse orientation splice acceptor for array) was designed with a promoter trap module (SAβgeo*pA; encoding a lacZ-neo fusion gene) and an frt-flanked poly-A trap module (PGKhygSD; PGK promoter-driven hygromycin gene with adenoviral splice donor). This construct was electroporated into 129S4/SvJaeSor-derived AK7.1 embryonic stem (ES) cells. ES cells with the ROSAFARY vector correctly targeted to the BC058969 region of chromosome 8 were identified and used to generate mutant mice. Homozygous mice were maintained on a C57BL/6 and 129S4/SvJaeSor mixed genetic background prior to arrival at The Jackson Laboratory.Of note, the primary publication and donating investigator report that when the mutation was made, blast results identified the gene trapped transcript as BC058969 on Chromosome 8. This is currently associated with the 2610005L07Rik gene (Dec-2007). Due to duplicated genomic sequence at this locus, the exact ROSAFARY insertion site is unknown. However, the ROSAFARY insertion point is reported to be in the first intron of the disrupted gene; upstream (and within 10 amino acids) of the coding start site.
The ROSAFARY vector inserts a promoterless lacZ-neo reporter fusion gene (which functions as an artificial 3' terminal exon to intercept and terminate transcription from the targeted gene promoter) as well as a poly-A trap (which functions as an artificial 5' terminal exon to initiate transcription from the insertion site). After inserting into an intron of an endogenous gene at a permissive site and in the correct orientation, the promoter trap module and the poly-A trap module can be activated to form fusion transcripts with the 5' or 3' exons.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (175 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
2610005L07RikGt(ROSA)73Sor/2610005L07RikGt(ROSA)73Sor
involves: 129S4/SvJaeSor
- lethality-prenatal/perinatal
- prenatal lethality (MGI Ref ID J:117491)
- few mice (9%) are born than expected (25%)
- skeleton phenotype
- abnormal skeleton morphology (MGI Ref ID J:117491)
- abnormal neural crest derived and thoracic skeleton
- abnormal calvaria morphology (MGI Ref ID J:117491)
- 100% of mice have reduced calvarial bones at the midline with increases in the gaps between frontal bones
- abnormal frontal bone morphology (MGI Ref ID J:117491)
- increases in the gaps between frontal bones
- abnormal sternum morphology (MGI Ref ID J:117491)
- 40% of mice have sternum defects including asymmetric or additional fusion of ribs and gaps in the sternum
- asymmetric rib-sternum attachment (MGI Ref ID J:117491)
- short maxilla (MGI Ref ID J:117491)
- short nasal bone (MGI Ref ID J:117491)
- short premaxilla (MGI Ref ID J:117491)
- cellular phenotype
- abnormal cell migration (MGI Ref ID J:117491)
- mouse embryonic fibroblast cells show increased migration in a scratch test in response to PDGF stimulation
- craniofacial phenotype
- abnormal calvaria morphology (MGI Ref ID J:117491)
- 100% of mice have reduced calvarial bones at the midline with increases in the gaps between frontal bones
- abnormal frontal bone morphology (MGI Ref ID J:117491)
- increases in the gaps between frontal bones
- abnormal snout morphology (MGI Ref ID J:117491)
- shorter and wider than in wild-type mice
- short maxilla (MGI Ref ID J:117491)
- short nasal bone (MGI Ref ID J:117491)
- short premaxilla (MGI Ref ID J:117491)
This mouse can be used to support research in many areas including:
Cardiovascular Research
Vascular Defects
Cell Biology Research
Defects in Cell Adhesion Molecules
Signal Transduction
Developmental Biology Research
Craniofacial and Palate Defects
Defects in Cell Adhesion Molecules
Embryonic Lethality (Homozygous) (incomplete)
Growth Defects
Internal/Organ Defects (kidney)
Internal/Organ Defects (multiple)
Internal/Organ Defects (vasculature)
Skeletal Defects
Internal/Organ Research
Kidney Defects
Research Tools
lacZ Expression
Cardiovascular Research (endothelial cell marker (lacZ) for neovascularization)
Cell Biology Research
Developmental Biology Research
Internal/Organ Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | 2610005L07RikGt(ROSA)73Sor | ||
|---|---|---|---|
| Allele Name | gene trapped 73, Philippe M Soriano | ||
| Allele Type | Gene trapped | ||
| Common Name(s) | BC058969-; | ||
| Mutation Made By | Philippe Soriano, Fred Hutchinson Cancer Research Center | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Site of Expression | Low b-galactosidase activity is reported; in situ hybridization or other sensitive methods may be necessary to detect expression of the lacZ-neo reporter fusion gene. | ||
| Gene Symbol and Name | 2610005L07Rik, RIKEN cDNA 2610005L07 gene | ||
| Chromosome | 8 | ||
| Gene Common Name(s) | 2810038F24Rik; 6720476A01Rik; RIKEN cDNA 2810038F24 gene; RIKEN cDNA 6720476A01 gene; | ||
| Molecular Note | The Rosafary gene trap vector containing a SAbetaGeo pA promoter trap module and a PGKhygSD poly-A trap module was inserted into the first intron of the locus. [MGI Ref ID J:117491] | ||
Genotyping
Genotyping Information
Genotyping Protocols
2610005L07RikGt(ROSA)73Sor, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Chen WV; Delrow J; Corrin PD; Frazier JP; Soriano P. 2004. Identification and validation of PDGF transcriptional targets by microarray-coupled gene-trap mutagenesis. Nat Genet 36(3):304-12. [PubMed: 14981515] [MGI Ref ID J:95727]
Schmahl J; Raymond CS; Soriano P. 2007. PDGF signaling specificity is mediated through multiple immediate early genes. Nat Genet 39(1):52-60. [PubMed: 17143286] [MGI Ref ID J:117491]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous or homozygous mice may be bred. Greater than half of all homozygous pups die in utero. Mating System Heterozygote x +/+ sibling (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Heterozygous for Cdh11Gt(ROSA)73Sor *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $472.80 Heterozygous for Cdh11Gt(ROSA)73Sor x Heterozygous for Cdh11Gt(ROSA)73Sor
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Heterozygous for Cdh11Gt(ROSA)73Sor *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $614.70 Heterozygous for Cdh11Gt(ROSA)73Sor x Heterozygous for Cdh11Gt(ROSA)73Sor
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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