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Strain Name:

129S-Myo1eGt(ROSA)74Sor/J

Stock Number:

007205

Availability:

Repository- Live

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Genes & Alleles   Myo1e;   Myo1eGt(ROSA)74Sor;

Product Information

Strain Details

Type JAX® GEMM® Strain - Gene Trap
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Stock
Type JAX® GEMM® Strain - Mutant Strain
Mating SystemHeterozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Philippe Soriano,   Fred Hutchinson Cancer Research Center

Strain Description
Homozygous embryos E11.5 to E18.5 exhibit hemorrhages and microaneurisms. Vascular defects persist into adulthood. At 6 weeks of age, mice are anemic (low hemoglobin concentration, red blood cell count, hematocrit). These mice also exhibit polychromasia (abnormally high number of immature blood cells). Homozygotes occur at lower than Mendelian ratio (15%). Although homozygotes are fertile, pregnancy is occasionally lethal for homozygous females. Heterozygotes are viable and fertile. No gene product is detected in homozygous embryos aged ED9.5-12.5 or in adult gonad. These Myo1e-mutant mice may be useful in studying vascular development, hematopoiesis and cellular signaling during development and in adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family) and immediate early genes (IEG) induced shortly after RTK activation.

Strain Development
The retroviral gene-trap vector ROSAFARY (or reverse orientation splice acceptor for array) was designed with a promoter trap module (SAbgeo*pA; encoding a lacZ-neo fusion gene) and an frt-flanked poly-A trap module (PGKhygSD; PGK promoter-driven hygromycin gene with adenoviral splice donor). This construct was electroporated into 129S4/SvJaeSor-derived AK7.1 embryonic stem (ES) cells. ES cells with the ROSAFARY vector correctly targeted to the Myo1e gene (in the 1st intron) were identified and used to generate mutant mice. Homozygous mice were maintained on a 129S4/SvJaeSor background prior to arrival at The Jackson Laboratory. The mice were then crossed to 129S1/SvImJ (Stock No. 002448) for one generation.

The ROSAFARY vector inserts a promoterless lacZ-neo reporter fusion gene (which functions as an artificial 3' terminal exon to intercept and terminate transcription from the targeted gene promoter) as well as a poly-A trap (which functions as an artificial 5' terminal exon to initiate transcription from the insertion site). After inserting into an intron of an endogenous gene at a permissive site and in the correct orientation, the promoter trap module and the poly-A trap module can be activated to form fusion transcripts with the 5' or 3' exons.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Myo1eGt(ROSA)74Sor/Myo1eGt(ROSA)74Sor

        involves: 129S4/SvJaeSor
  • lethality-prenatal/perinatal
  • prenatal lethality (MGI Ref ID J:117491)
    • few mice (15%) are born than expected (25%)
  • cardiovascular system phenotype
  • abnormal vasculature (MGI Ref ID J:117491)
  • hemorrhage (MGI Ref ID J:117491)
    • at E11.5-E18.5
  • hematopoietic system phenotype
  • abnormal red blood cell (MGI Ref ID J:117491)
    • an increase in immature red blood cells is observed compared to wild type mice
    • anemia (MGI Ref ID J:117491)
      • by week 6
    • decreased erythrocyte cell number (MGI Ref ID J:117491)
      • by week 6
    • decreased hematocrit (MGI Ref ID J:117491)
      • by week 6
    • decreased hemoglobin content (MGI Ref ID J:117491)
      • by week 6
    • polychromatophilia (MGI Ref ID J:117491)

Gene & Allele Details

Allele Symbol Myo1eGt(ROSA)74Sor
Allele Name gene trapped 74, Philippe M Soriano
Common Name(s) Myo1e-;
Mutation Made By Philippe Soriano,   Fred Hutchinson Cancer Research Center
Strain of Origin129S4/SvJaeSor
ES Cell Line NameAK7
ES Cell Line Strain129S4/SvJaeSor
Gene Symbol and Name Myo1e, myosin IE
Chromosome 9
Gene Common Name(s) 2310020N23Rik; 9130023P14Rik; AA407778; HuncM-IC; MGC104638; MYO1C; MYR5; Myr3; RIKEN cDNA 2310020N23 gene; RIKEN cDNA 9130023P14 gene; expressed sequence AA407778;
Molecular Note The Rosafary gene trap vector containing a SAbetaGeo pA promoter trap module and a PGKhygSD poly-A trap module was isnerted into the first intron of the locus. [MGI Ref ID J:117491]

Control Information

  Control
   002448 129S1/SvImJ (approximate)
 
  Considerations for Choosing Controls

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes occur at lower than Mendelian ratio (15%). Although homozygotes are fertile, pregnancy is occasionally lethal for homozygous females. The Donating Investigator used a breeding scheme of homozygous male x heterozygous female.
Diet Information LabDiet® 5K52/5K67

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Cardiovascular Research
Vascular Defects

Cell Biology Research
Signal Transduction

Developmental Biology Research
Embryonic Lethality (Homozygous) (incomplete)
Internal/Organ Defects (hematopoietic defects)

Hematological Research
Hematopoietic Defects

References

Selected Reference(s)

Schmahl J; Raymond CS; Soriano P. 2007. PDGF signaling specificity is mediated through multiple immediate early genes. Nat Genet 39(1):52-60. [PubMed: 17143286]  [MGI Ref ID J:117491]

Price and Supply Information

Strain Name: 129S-Myo1eGt(ROSA)74Sor/J
Stock Number: 007205

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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