Description

Strain Information

Type Gene Trap; Mutant Stock; Additional information on Genetically Engineered Mutant Mice. Mating System Heterozygote x Heterozygote         (Female x Male) Species laboratory mouse Generation ?+F3 (13-OCT-08)   Donating Investigator Philippe Soriano,   Mount Sinai School of Medicine

Description
Homozygous embryos E11.5 to E18.5 exhibit hemorrhages and microaneurisms. Vascular defects persist into adulthood. At 6 weeks of age, mice are anemic (low hemoglobin concentration, red blood cell count, hematocrit). These mice also exhibit polychromasia (abnormally high number of immature blood cells); kidney defects (blood urea nitrogen level abnormally high, kidney size smaller than wildtype, swollen blood filled, degraded glomeruli are often observed, increased number of vascular smooth muscle cells partially filling the glomeruli space); and abnormalities in palate bone fusion. Homozygotes occur at lower than Mendelian ratio (22%), and 32% die by age 2 week. Heterozygotes are viable and fertile. No gene product is detected in homozygous embryos aged ED9.5-12.5 or in adult gonad. These Tiparp-mutant mice may be useful in studying kidney development, vascular development, hematopoiesis and cellular signaling during development and in adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family) and immediate early genes (IEG) induced shortly after RTK activation.

Development
The retroviral gene-trap vector ROSAFARY (or reverse orientation splice acceptor for array) was designed with a promoter trap module (SAβgeo*pA; encoding a lacZ-neo fusion gene) and an frt-flanked poly-A trap module (PGKhygSD; PGK promoter-driven hygromycin gene with adenoviral splice donor). This construct was electroporated into 129S4/SvJaeSor-derived AK7.1 embryonic stem (ES) cells. ES cells with the ROSAFARY vector correctly targeted to the Tiparp gene (in the 1st intron) were identified and used to generate mutant mice. These mutant mice were maintained on a mixed B6;129S4 background prior to arrival at The Jackson Laboratory. The mice were then crossed to C57BL/6J (Stock No. 000664) for one generation.

The ROSAFARY vector inserts a promoterless lacZ-neo reporter fusion gene (which functions as an artificial 3' terminal exon to intercept and terminate transcription from the targeted gene promoter) as well as a poly-A trap (which functions as an artificial 5' terminal exon to initiate transcription from the insertion site). After inserting into an intron of an endogenous gene at a permissive site and in the correct orientation, the promoter trap module and the poly-A trap module can be activated to form fusion transcripts with the 5' or 3' exons.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tiparp^{Gt(ROSA)79Sor}/Tiparp^{Gt(ROSA)79Sor}

        involves: 129S4/SvJaeSor

• lethality-postnatal
• postnatal lethality (MGI Ref ID J:117491)
  • 32% of mice die by week 2
• skeleton phenotype
• abnormal skeleton morphology (MGI Ref ID J:117491)
  • abnormal neural crest derived and thoracic skeleton
• abnormal calvaria morphology (MGI Ref ID J:117491)
  • 55% of mice have reduced calvarial bones at the midline with increases in the gaps between frontal bones
• abnormal frontal bone morphology (MGI Ref ID J:117491)
  • increases in the gaps between frontal bones
• abnormal palatine shelf (MGI Ref ID J:117491)
  • 78% of mice have smaller and less extended towards the midline palatine and presphenoid bones
• abnormal presphenoid bone morphology (MGI Ref ID J:117491)
  • 78% of mice have smaller and less extended towards the midline palatine and presphenoid bones
• abnormal sternum morphology (MGI Ref ID J:117491)
  • 44% of mice have sternum defects including asymmetric or additional fusion of ribs and gaps in the sternum
• asymmetric rib-sternum attachment (MGI Ref ID J:117491)
• short maxilla (MGI Ref ID J:117491)
• short nasal bone (MGI Ref ID J:117491)
• short premaxilla (MGI Ref ID J:117491)
• renal/urinary system phenotype
• abnormal kidney morphology (MGI Ref ID J:117491)
• abnormal renal glomerulus morphology (MGI Ref ID J:117491)
  • glomeruli are often degraded
  • excess smooth muscle cells are found in the glomeruli
• pale kidney (MGI Ref ID J:117491)
• small kidney (MGI Ref ID J:117491)
• kidney inflammation (MGI Ref ID J:117491)
  • kidney is swollen in sections and blood filled
• hematopoietic system phenotype
• abnormal red blood cell (MGI Ref ID J:117491)
  • an increase in immature red blood cells is observed compared to wild-type mice
• anemia (MGI Ref ID J:117491)
  • by week 6
• decreased erythrocyte cell number (MGI Ref ID J:117491)
  • by week 6
• decreased hematocrit (MGI Ref ID J:117491)
  • by week 6
• decreased hemoglobin content (MGI Ref ID J:117491)
  • by week 6
• polychromatophilia (MGI Ref ID J:117491)
• homeostasis/metabolism phenotype
• increased blood urea nitrogen level (MGI Ref ID J:117491)
• cardiovascular system phenotype
• abnormal vasculature (MGI Ref ID J:117491)
• hemorrhage (MGI Ref ID J:117491)
  • at E11.5-E18.5
• muscle phenotype
• abnormal smooth muscle morphology (MGI Ref ID J:117491)
  • excess smooth muscle cells are found in the glomeruli
• immune system phenotype
• kidney inflammation (MGI Ref ID J:117491)
  • kidney is swollen in sections and blood filled
• craniofacial phenotype
• abnormal calvaria morphology (MGI Ref ID J:117491)
  • 55% of mice have reduced calvarial bones at the midline with increases in the gaps between frontal bones
• abnormal frontal bone morphology (MGI Ref ID J:117491)
  • increases in the gaps between frontal bones
• abnormal palate morphology (MGI Ref ID J:117491)
  • irregularities in fusion persist into adulthood
• abnormal palatine shelf (MGI Ref ID J:117491)
  • 78% of mice have smaller and less extended towards the midline palatine and presphenoid bones
• abnormal presphenoid bone morphology (MGI Ref ID J:117491)
  • 78% of mice have smaller and less extended towards the midline palatine and presphenoid bones
• abnormal snout morphology (MGI Ref ID J:117491)
  • shorter and wider than in wild-type mice
• short maxilla (MGI Ref ID J:117491)
• short nasal bone (MGI Ref ID J:117491)
• short premaxilla (MGI Ref ID J:117491)
• digestive/alimentary phenotype
• abnormal palate morphology (MGI Ref ID J:117491)
  • irregularities in fusion persist into adulthood
• abnormal palatine shelf (MGI Ref ID J:117491)
  • 78% of mice have smaller and less extended towards the midline palatine and presphenoid bones

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cardiovascular Research
Vascular Defects

Cell Biology Research
Signal Transduction

Developmental Biology Research
Craniofacial and Palate Defects
Embryonic Lethality (Homozygous) (incomplete)
Internal/Organ Defects (kidney)
Perinatal Lethality (Homozygous)

Hematological Research
Hematopoietic Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		TiparpGt(ROSA)79Sor
Allele Name		gene trapped 79, Philippe M Soriano
Allele Type		Gene trapped
Common Name(s)		Tiparp-;
Mutation Made By		Philippe Soriano,   Fred Hutchinson Cancer Research Center
Strain of Origin		129S4/SvJaeSor
ES Cell Line Name		AK7
ES Cell Line Strain		129S4/SvJaeSor
Gene Symbol and Name		Tiparp, TCDD-inducible poly(ADP-ribose) polymerase
Chromosome		3
Gene Common Name(s)		AW558171; DDF1; DKFZp434J214; DKFZp686N0351; DKFZp686P1838; FLJ40466; PARP-1; PARP-7; PARP7; expressed sequence AW558171;
Molecular Note		The Rosafary gene trap vector containing a SAbetaGeo pA promoter trap module and a PGKhygSD poly-A trap module was inserted into intron 1. [MGI Ref ID J:117491]

Genotyping

Genotyping Information

Genotyping Protocols

Tiparp^{Gt(ROSA)79Sor}, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Schmahl J; Raymond CS; Soriano P. 2007. PDGF signaling specificity is mediated through multiple immediate early genes. Nat Genet 39(1):52-60. [PubMed: 17143286]  [MGI Ref ID J:117491]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes occur at lower than Mendelian ratio (22%), and 32% die by age 2 week. The Donating Investigator used a breeding scheme of homozygous male x heterozygous female.
Mating System	Heterozygote x Heterozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.