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Strain Name:

B6(Cg)-Tnfrsf13ctm1Mass/J

Stock Number:

007212

Availability:

Repository- Live


General Terms and Conditions

Genes & Alleles   Tnfrsf13c;   Tnfrsf13ctm1Mass;


Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Klaus Rajewsky,   Harvard Medical School
Generation?+F2 (08-JAN-08)

Strain Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by RT-PCR or FACS analysis of spleen tissue and cells, respectively. Homozygotes have reduced numbers of mature recirculating bone marrow and splenic B cells. B cell development is arrested between the transitional IgM+ (T1 + T2) and IgMlow (T3) stages. Homozygotes exhibit diminished antigen-specific antibody responses with decreased levels of IgM, IgG1, IgG2, IgG2b and IgG3. This mutant mouse strain may be useful in studies of B cell development and differentiation.

Strain Development
A targeting vector was used to insert loxP sites on either side of exons 3 and 4, and a FRT site flanked neomycin resistance cassette. The construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c recipient blastocysts. All mice were maintained on a pure C57BL/6 background. The resulting chimeric animals were crossed to C57BL/6 transgenic mice expressing Cre recombinase under the control of the cytomegalovirus promoter, to remove the floxed exons. The donating investigator reports that the mice do not carry the cre transgene.

Mammalian Phenotype Terms assigned by genotype

Tnfrsf13ctm1Mass/Tnfrsf13ctm1Mass

        C57BL/6-Tnfrsf13ctm1Mass
  • hematopoietic system phenotype
  • arrested B cell differentiation (J:92732)
    • a block in B cell development from IgM+ (T1 + T2) to IgMlow (T3) cells is seen
  • decreased mature B cell number (J:92732)
    • the number of mature recirculating B cells is reduced with about a 10-fold reduction in IgD+ B cells in the bone marrow and a 20-fold decrease in IgMlowIgD+ B cells in the lymph nodes
    • absent marginal zone B cells (J:92732)
    • decreased B-2 cell number (J:92732)
      • a reduction in peritoneal B2 cells but not B1 cells is seen
  • immune system phenotype
  • arrested B cell differentiation (J:92732)
    • a block in B cell development from IgM+ (T1 + T2) to IgMlow (T3) cells is seen
  • decreased IgG level (J:92732)
    • the titers of IgG1, IgG2a, IgG2b, and IgG3 are reduced
    • decreased IgG1 level (J:92732)
      • immunization with NP-CG produces a significantly smaller increase in IgG1 titers in mutants compared to wild type mice and no secondary NP-specific response is detected in mutants
    • decreased IgG2a level (J:92732)
    • decreased IgG2b level (J:92732)
    • decreased IgG3 level (J:92732)
      • immunization with NP-Ficoll produces a smaller increase in IgG3 titers in mutants compared to wild type mice
  • decreased IgM level (J:92732)
    • the titer of IgM but not IgA is reduced compared to wild type mice
    • immunization with NP-Ficoll produces a smaller increase in IgM titers in mutants compared to wild type mice
  • decreased mature B cell number (J:92732)
    • the number of mature recirculating B cells is reduced with about a 10-fold reduction in IgD+ B cells in the bone marrow and a 20-fold decrease in IgMlowIgD+ B cells in the lymph nodes
    • absent marginal zone B cells (J:92732)
    • decreased B-2 cell number (J:92732)
      • a reduction in peritoneal B2 cells but not B1 cells is seen

Gene & Allele Details

Allele Symbol Tnfrsf13ctm1Mass
Allele Name targeted mutation 1, Marc Schmidt-Supprian
Common Name(s) Baff-r-;
Mutation Made By Klaus Rajewsky,   Harvard Medical School
Strain of OriginC57BL/6
ES Cell Line NameBruce 4
ES Cell Line StrainC57BL/6
Gene Symbol and Name Tnfrsf13c, tumor necrosis factor receptor superfamily, member 13c
Chromosome 15
Gene Common Name(s) 2010006P15Rik; B-cell maturation defect 1; BAFF-R; BAFFR; Bcmd-1; Bcmd1; CD268; Lvis22; MGC138235; RGD1560810; RIKEN cDNA 2010006P15 gene;
Molecular Note Cre-recombinase excised a floxed region containing exons 3 and 4. The excised region encoded the entire extracellular and transmembrane domains and part of the cytoplasmic domain. RT-PCR did not detect mRNA in mutants. FACS analysis showed no surface expression of mutant splenic cells. [J:92732]

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Tnfrsf13ctm1Mass

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice may be bred as homozygotes.
Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying other alleles of Tnfrsf13c
000647   A/WySnJ
View Strains carrying other alleles of Tnfrsf13c     (1 strain)

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Immunology and Inflammation Research
Immunodeficiency (B cell deficiency)

Research Tools
Immunology and Inflammation Research (B cell deficiency)

References

Selected Reference(s)

Sasaki Y; Casola S; Kutok JL; Rajewsky K; Schmidt-Supprian M. 2004. TNF family member B cell-activating factor (BAFF) receptor-dependent and -independent roles for BAFF in B cell physiology. J Immunol 173(4):2245-52. [PubMed: 15294936]  [J:92732]

Additional References

Price and Supply Information

Strain Name: B6(Cg)-Tnfrsf13ctm1Mass/J
Stock Number: 007212

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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