Strain Name: |
B6(Cg)-Tnfrsf13ctm1Mass/J |
|---|---|
Stock Number: |
007212 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Tnfrsf13c; Tnfrsf13ctm1Mass; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Klaus Rajewsky, Harvard Medical School Generation ?+F2 (08-JAN-08) Strain Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by RT-PCR or FACS analysis of spleen tissue and cells, respectively. Homozygotes have reduced numbers of mature recirculating bone marrow and splenic B cells. B cell development is arrested between the transitional IgM+ (T1 + T2) and IgMlow (T3) stages. Homozygotes exhibit diminished antigen-specific antibody responses with decreased levels of IgM, IgG1, IgG2, IgG2b and IgG3. This mutant mouse strain may be useful in studies of B cell development and differentiation.Strain Development
A targeting vector was used to insert loxP sites on either side of exons 3 and 4, and a FRT site flanked neomycin resistance cassette. The construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c recipient blastocysts. All mice were maintained on a pure C57BL/6 background. The resulting chimeric animals were crossed to C57BL/6 transgenic mice expressing Cre recombinase under the control of the cytomegalovirus promoter, to remove the floxed exons. The donating investigator reports that the mice do not carry the cre transgene.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tnfrsf13ctm1Mass | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Marc Schmidt-Supprian | ||
| Common Name(s) | Baff-r-; | ||
| Mutation Made By | Klaus Rajewsky, Harvard Medical School | ||
| Strain of Origin | C57BL/6 | ||
| ES Cell Line Name | Bruce 4 | ||
| ES Cell Line Strain | C57BL/6 | ||
| Gene Symbol and Name | Tnfrsf13c, tumor necrosis factor receptor superfamily, member 13c | ||
| Chromosome | 15 | ||
| Gene Common Name(s) | 2010006P15Rik; B-cell maturation defect 1; BAFF-R; BAFFR; Bcmd-1; Bcmd1; CD268; Lvis22; MGC138235; RGD1560810; RIKEN cDNA 2010006P15 gene; | ||
| Molecular Note | Cre-recombinase excised a floxed region containing exons 3 and 4. The excised region encoded the entire extracellular and transmembrane domains and part of the cytoplasmic domain. RT-PCR did not detect mRNA in mutants. FACS analysis showed no surface expression of mutant splenic cells. [J:92732] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tnfrsf13ctm1Mass
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice may be bred as homozygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Tnfrsf13c
000647 A/WySnJ View Strains carrying other alleles of Tnfrsf13c (1 strain)
Additional Web Information
Congenic Nomenclature
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
Immunodeficiency (B cell deficiency)
Research Tools
Immunology and Inflammation Research (B cell deficiency)
References
Selected Reference(s)
Additional ReferencesSasaki Y; Casola S; Kutok JL; Rajewsky K; Schmidt-Supprian M. 2004. TNF family member B cell-activating factor (BAFF) receptor-dependent and -independent roles for BAFF in B cell physiology. J Immunol 173(4):2245-52. [PubMed: 15294936] [J:92732]
Price and Supply Information
| Strain Name: | B6(Cg)-Tnfrsf13ctm1Mass/J |
| Stock Number: | 007212 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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