Strain Name: |
B6.129S6-Trp53tm2Xu/J |
|---|---|
Stock Number: |
007218 |
Availability: | Under Development for Distribution Colony |
| To register your interest in this strain go to the Strain Interest Form. |
General Terms and Conditions |
| Former Name |
B6.129S-Trp53tm2Xu/J (Changed: 15-JUL-08
) |
| Genes & Alleles | Trp53; Trp53tm2Xu; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System +/+ sibling x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation ?+N5 (28-JUL-08) Strain Description
Mice homozygous for this targeted mutation are viable and fertile. They carry two phosphorylation site disruptions: Ser18 to Ala (S18A), and Ser23 to Ala (S23A). Trp53-dependent apoptosis is essentially abolished in thymocytes. These mice develop spontaneous tumors at approximately 1.5 years of age and also develop a range of malignancies that are distinct from those found in animals carrying either a single S23A mutation or a complete knockout of the gene. This mutant mouse strain may be useful in studies of phosphorylation events, cancer development and apoptosis.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A targeting vector containing Ser18 to Ala, and Ser23 to Ala mutations, in addition to a "floxed" PGK-Neo resistance cassette in exon 4, was injected into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. F1 mutant heterozygous mice were bred to CMV-Cre transgenic mice to excise the "floxed" PGK-Neo cassette. Homozyotes were created by intercrossing heterozygous mice prior to arrival at The Jackson Laboratory (as Stock No. 006980). Upon arrival, some mice were backcrossed to C57BL/6J for at least 5 generations to generate this congenic strain (Stock No. 007218).
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Trp53tm2Xu | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Yang Xu | ||
| Common Name(s) | p53S18/23A; | ||
| Mutation Made By | Yang Xu, University of California, San Diego | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | TC-1 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Gene Symbol and Name | Trp53, transformation related protein 53 | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | FLJ92943; LFS1; MGC112612; Tp53; p53; | ||
| Molecular Note | Site directed mutagenesis within exon 2 generated missense mutations at codons 18 and 23, resulting in the substitutions of both serines with alanines. A single loxP site remained in intron 4 after a floxed PGK-neo cassette was excised via cre-mediated recombination. Sequencing verified that only the S18/23A mutations were present in mutant mice. [MGI Ref ID J:109354] | ||
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Trp53tm2Xu
| Breeding & Husbandry | Mutant mice were bred to C57BL/6J mice to generate this congenic strain. When maintaining the live congenic colony, heterozygous mice are bred to wildtype siblings or to C57BL/6J inbred mice. Homozygous mice may also be bred. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Strains carrying Trp53tm2Xu allele
006980 B6;129-Trp53tm2Xu/J View Strains carrying Trp53tm2Xu (1 strain)
Strains carrying other alleles of Trp53
004301 129-Trp53tm1Holl/J 002080 129-Trp53tm1Tyj/J 002101 B6.129S2-Trp53tm1Tyj/J 007962 B6.FVB-Tg(MMTV-neu/OT-I/OT-II)CBnel Tg(Trp53R172H)8512Jmr/J 002103 B6;129S2-Trp53tm1Tyj/J 002526 C.129S2(B6)-Trp53tm1Tyj/J 002547 C3Ou.129S2(B6)-Trp53tm1Tyj/J 002899 FVB.129S2(B6)-Trp53tm1Tyj/J 002659 FVB/N-Tg(Trp53R172H)8512Jmr/J 002660 FVB/N-Tg(Trp53R172L)4491Jmr/J 003262 STOCK Tg(Trp53A135V)L3Ber/J View Strains carrying other alleles of Trp53 (11 strains)
Congenic Nomenclature
Trp53 relatedApoptosis Research
Endogenous Regulators
Cancer Research
Increased Tumor Incidence (Adenomas)
Increased Tumor Incidence (Gonadal Tumors: ovarian)
Increased Tumor Incidence (Leukemia)
Increased Tumor Incidence (Lymphomas)
Increased Tumor Incidence (Other Tissues/Organs)
Tumor Suppressor Genes
Immunology and Inflammation Research
Intracellular Signaling Molecules
Apoptosis Research
Endogenous Regulators
Cancer Research
Increased Tumor Incidence (Lymphomas)
Increased Tumor Incidence (Other Tissues/Organs: osteosarcoma)
Toxicology
Tumor Suppressor Genes
Immunology and Inflammation Research
Intracellular Signaling Molecules
Mouse/Human Gene Homologs
Li-Fraumeni syndrome
Research Tools
Toxicology Research (B and T cell deficiency) (xenograft transplant host)
Toxicology Research (drug/compound testing)
Selected Reference(s)
Chao C; Herr D; Chun J; Xu Y. 2006. Ser18 and 23 phosphorylation is required for p53-dependent apoptosis and tumor suppression. EMBO J 25(11):2615-22. [PubMed: 16757976] [MGI Ref ID J:109354]
| Strain Name: | B6.129S6-Trp53tm2Xu/J |
| Stock Number: | 007218 |
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 15-SEP-08
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
OncoMouse® requires a license from DuPont, see Licenses for Strains with OncoMouse® Technology.
P53 Mice are subject to U.S. 5,569,824 and corresponding license requirements.
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