Strain Name: |
B6.Cg-Apoetm1Unc(D12Mit182-D12Mit2)/Pgn |
|---|---|
Stock Number: |
007224 |
Availability: | Repository-Cryopreserved |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Apoe; Apoetm1Unc; Ath6; Ath6C57BLKS/J; D12Mit182; D12Mit2; ; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse H2 Haplotype b Generation F6pN1 Strain Development
This strain was created by mating B6.129P2-Apoetm1Unc/J (Stock No. 002052) to B6.BKS-(D12Mit182-D12Mit2)/Pgn (Stock No. 006056). The colony is homozygous for Apoetm1Unc and Ath6C57BLKS/J, which is located within the D12Mit182-D12Mit2 interval. Dr. Beverly Paigen generated this strain at The Jackson Laboratory; the strain was cryopreserved in 2008.
Gene & Allele Details
| Allele Symbol | Apoetm1Unc | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, University of North Carolina | ||
| Common Name(s) | APOE KO; AopE(-); ApoE-KO; Apoetm1Un; apoE-; apoE0; epsilon-; | ||
| Mutation Made By | Nobuyo Maeda, Univ of North Carolina at Chapel Hill | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14TG2a | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Apoe, apolipoprotein E | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | AD2; AI255918; LPG; MGC1571; apoprotein; expressed sequence AI255918; | ||
| General Note |
In Apoetm1Unc mutant mice also carrying Csf1op, atherotic lesions of the proximal aorta were abolished or significantly reduced in size relative to Apoe mutants homozygous for the normal allele at Csf1 (J:40136). The results may have been due to reduced macrophages or M-CSF, but were not due to reductions in circulating lipoprotein (J:40136). | ||
| Molecular Note | Insertion of a neomycin resistance cassette deleted part of exon 3 and part of intron 3 of the Apoe gene. Plasma from homozygous mutant mice gave no detectable immunoprecipitate by the Ouchterlony double immunodiffusion test using a rabbit antibody to rat APOE. [MGI Ref ID J:1050] | ||
| Gene Symbol and Name | D12Mit182, DNA segment, Chr 12, Massachusetts Institute of Technology 182 | ||
| Chromosome | 12 | ||
| Gene Symbol and Name | D12Mit2, DNA segment, Chr 12, Massachusetts Institute of Technology 2 | ||
| Chromosome | 12 | ||
| Allele Symbol | Ath6C57BLKS/J | ||
| Allele Name | C57BLKS/J | ||
| Strain of Origin | C57BLKS/J | ||
| Gene Symbol and Name | Ath6, atherosclerosis 6 | ||
| Chromosome | 12 | ||
Genotyping Protocols
Apoetm1Unc
Related Strains
Strains carrying Apoetm1Unc allele
View Strains carrying Apoetm1Unc (9 strains)
006056 B6.BKS-(D12Mit182-D12Mit2)/Pgn
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:
Apoetm1Unc relatedCardiovascular Research
Atherosclerosis
Research Tools
Genetics Research (Gene Mapping: Tools for QTL Mapping, Segregation and Linkage Analysis)
Cardiovascular Research
Atherosclerosis
Hypercholesterolemia
Mouse/Human Gene Homologs
Alzheimer's
hyperlipoproteinemia, type III
References
Additional ReferencesPrice and Supply Information
| Strain Name: | B6.Cg-Apoetm1Unc(D12Mit182-D12Mit2)/Pgn |
| Stock Number: | 007224 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | |||||
|---|---|---|---|---|---|
| Cryorecovery Fee | $2470.00 | ||||
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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