Strain Name: |
B6.B10ScN-Tlr4lps-del/JthJ |
|---|---|
Stock Number: |
007227 |
Availability: | Under Development for Distribution Colony |
| To register your interest in this strain go to the Strain Interest Form. |
General Terms and Conditions |
| Genes & Alleles | Tlr4; Tlr4lps-del; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Spontaneous Mutation Mating System +/+ sibling x Heterozygote (Female x Male) Species laboratory mouse Background Strain C57BL/6 Donor Strain C57BL/10ScN H2 Haplotype b Generation N6+ (12-AUG-08) Appearance
black
Related Genotype: a/aStrain Description
This spontaneous mutation is a 7 kb deletion in the Tlr4 gene, which results in absence of both mRNA and protein and thus exhibits a defective response to LPS stimulation. The functionally similar Tlr4Lps-d mutation found in C3H/HeJ mice is a point mutation that causes an amino acid substitution. The allele for normal LPS response, Tlr4lps-n, occurs in most other C3H and C57BL/10 substrains and most mouse strains. This strain may be used to study the Toll signalling pathway and susceptibility to Gram-negative bacterial infection.Strain Development
Abnormal response to lipopolysaccharide was observed in the inbred strain C57BL/10ScN in the 1970s. The phenotype was subsequently identified as a mutation in the Tlr4 gene. This strain originated from the C57BL/10ScN colony at NCI Frederick and was transferred to the laboratory of Dr. James Thomas, University of Texas Southwestern Medical Center. The allele was introgressed into C57BL/6 by backcrossing for at least five generations. The strain was donated to The Jackson Laboratory Repository in 2008.
Related Disease (OMIM) Terms |
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Tlr4lps-del | ||
|---|---|---|---|
| Allele Name | defective lipopolysaccharide response, deletion | ||
| Common Name(s) | Tlr4-; | ||
| Strain of Origin | C57BL/10ScN | ||
| Gene Symbol and Name | Tlr4, toll-like receptor 4 | ||
| Chromosome | 4 | ||
| Gene Common Name(s) | ARMD10; CD284; Lps; RAS-like, family 2, locus 8; Rasl2-8; TOLL; hToll; lipopolysaccharide response; | ||
| General Note | C57BL/10ScN mice carry this allele. | ||
| Molecular Note | This allele has been characterized by lack of mRNA owing to a deletion of the locus. 74723bp of genomic DNA sequence have been removed, apparently encompasing only the Tlr4 gene. [MGI Ref ID J:122203] [MGI Ref ID J:51522] | ||
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as homozygotes. |
|---|
Strains carrying Tlr4lps-del allele
003752 C57BL/10ScNJ View Strains carrying Tlr4lps-del (1 strain)
Strains carrying other alleles of Tlr4
002930 C.C3-Tlr4Lps-d/J 005973 C3Bir.129P2(B6)-Il10C3Bir/LtJ 004326 C3Bir.129P2(B6)-Il10tm1Cgn/Lt 003968 C3Bir.129P2(B6)-Il10tm1Cgn/LtJ 000659 C3H/HeJ 005972 C3H/HeJBirLtJ View Strains carrying other alleles of Tlr4 (6 strains)
Congenic Nomenclature
Tlr4lps-del relatedImmunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers (Tlr deficiency)
Immunodeficiency (Tlr deficiency)
Inflammation (Tlr deficiency)
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers (Tlr deficiency)
Immunodeficiency (Tlr deficiency)
Inflammation (Tlr deficiency)
Selected Reference(s)
Additional ReferencesPoltorak A; He X; Smirnova I; Liu MY; Huffel CV; Du X; Birdwell D; Alejos E; Silva M; Galanos C; Freudenberg M; Ricciardi-Castagnoli P; Layton B; Beutler B. 1998. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science 282(5396):2085-8. [PubMed: 9851930] [MGI Ref ID J:51522]
Vogel SN; Hansen CT; Rosenstreich DL. 1979. Characterization of a congenitally LPS-resistant, athymic mouse strain. J Immunol 122(2):619-22. [PubMed: 368244] [MGI Ref ID J:6086]
| Strain Name: | B6.B10ScN-Tlr4lps-del/JthJ |
| Stock Number: | 007227 |
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
This strain is included in the Mouse Mutant Resource collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
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