Description

Strain Information

Type Congenic; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male)   28-APR-09 Species laboratory mouse Background Strain C57BL/6 Donor Strain C57BL/10ScN H2 Haplotype b Generation N6+N1F7 (24-MAR-11) Generation Definitions

Appearance
black
Related Genotype: a/a

Description
    The stimulation of Toll-like receptor 4 (TLR4) by lipopolysaccharide (LPS) induces the release of proinflammatory cytokines that activate immune responses. The Tlr4^Lps-del spontaneous mutation has a 7 kb deletion in the Tlr4 gene that results in absence of both mRNA and protein and thus exhibits a defective response to LPS stimulation. The functionally similar Tlr4^Lps-d mutation found in C3H/HeJ mice (#000659) is a point mutation that causes an amino acid substitution.
    Tlr4 -deficient mice display significantly reduced expression of proinflammatory genes compared to controls 24 h after reperfusion triggered by retinal ischemic injury. These include transcriptional factor p65 (Rela), tumor necrosis factor (Thf), interleukin 6 (Il6), chemokine (C-C motif) ligand 2 (Ccl2), chemokine (C-C motif) ligand 5 (Ccl5), chemokine (C-X-C motif) ligand 10 (Cxcl10), cytochrome b-245, beta polypeptide (Cybb), nitric oxide synthase 2 (Nos2), and intercellular adhesion molecule 1 (Icam1) (Dvoriantchikova et al., 2010).
    The effect of TLR4 on tumor progression appears to depend on which cells TLR4 resides and the particular ligand involved. For example, enhanced prostrate tumor progression as a result of TLR4 response to a tumor-derived ligand such as Peroxiredoxin is not seen in the Tlr4 deficient mutant mice (Riddell et al., 2011). Conversely, tumor inhibition seen in wild type mice when B16 melanoma cells were stimulated in vitro with LPS was not seen in mutant mice (Nunez et al., 2012).
    TLR4 signaling may also act as innate neuroprotective mechanism through clearance of alpha-synuclein as TLR4 ablation impairs the phagocytic response of microglia to alpha-synuclein and enhances neurodegeneration (Stefanova et al. 2011).

Development
Abnormal response to lipopolysaccharide was observed in the inbred strain C57BL/10ScN in the 1970s. The phenotype was subsequently identified as a mutation in the Tlr4 gene. This strain originated from the C57BL/10ScN colony at NCI Frederick and was transferred to the laboratory of Dr. James Thomas, University of Texas Southwestern Medical Center. The allele was introgressed into C57BL/6 by backcrossing for at least five generations. The strain was donated to The Jackson Laboratory Repository in 2008.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Tlr4^lps-del allele

003752

C57BL/10ScNJ

View Strains carrying   Tlr4^lps-del     (1 strain)

Strains carrying other alleles of Tlr4

000029	BXD29-Tlr4lps-2J/J
002930	C.C3-Tlr4Lps-d/J
005973	C3Bir.129P2(B6)-Il10C3Bir/LtJ
004326	C3Bir.129P2(B6)-Il10tm1Cgn/Lt
003968	C3Bir.129P2(B6)-Il10tm1Cgn/LtJ
000659	C3H/HeJ
005972	C3H/HeJBirLtJ

View Strains carrying other alleles of Tlr4     (7 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

Toll-Like Receptor 4; TLR4

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Colorectal Cancer; CRC   (TLR4) Macular Degeneration, Age-Related, 10; ARMD10   (TLR4)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tlr4^lps-del/Tlr4^lps-del

        C57BL/10ScCr

• immune system phenotype
• abnormal cytokine secretion
  • higher levels of Th1 and Th2 cytokines secreted at 4 weeks after infection with Leishmania major than in controls   (MGI Ref ID J:88996)
• abnormal macrophage physiology
  • poor activation of macrophage by Leishmania major   (MGI Ref ID J:88996)
• increased susceptibility to bacterial infection
  • response to endotoxin (LPS) prevented   (MGI Ref ID J:51522)
  • Leishmania major parasitic loads significantly higher than controls starting 24 hours after infection and persisting to 11 weeks after infection when controls are free of parasites   (MGI Ref ID J:88996)
• behavior/neurological phenotype
• hyporesponsive to tactile stimuli
  • attenuated mechanical allodynia after nerve injury   (MGI Ref ID J:97819)
• increased thermal nociceptive threshold
  • attenuated response to heat   (MGI Ref ID J:97819)
• nervous system phenotype
• abnormal glial cell physiology
  • glial activation after nerve injury reduced   (MGI Ref ID J:97819)
• cardiovascular system phenotype
• decreased response of heart to induced stress
  • exhibit reduced cardiac hypertrophy following pressure overload compared to controls   (MGI Ref ID J:106358)
• homeostasis/metabolism phenotype
• decreased susceptibility to injury
  • reduced spinal expression of TNF alpha, IFN gamma, IL-6 and IL-1 after injury   (MGI Ref ID J:97819)
  • glial activation after nerve injury reduced   (MGI Ref ID J:97819)
• • decreased response of heart to induced stress
    • exhibit reduced cardiac hypertrophy following pressure overload compared to controls   (MGI Ref ID J:106358)
• integument phenotype
• hyporesponsive to tactile stimuli
  • attenuated mechanical allodynia after nerve injury   (MGI Ref ID J:97819)
• increased thermal nociceptive threshold
  • attenuated response to heat   (MGI Ref ID J:97819)

Tlr4^lps-del/Tlr4^lps-del

        C57BL/10ScN

• digestive/alimentary phenotype
• rectal hemorrhage
  • rectal bleeding after 7 days of dextran sodium sulfate treatment is reduced relative to controls   (MGI Ref ID J:37271)
• immune system phenotype
• increased spleen weight
  • spleen weight elevated with dextran sodium sulfate treatment relative to controls   (MGI Ref ID J:37271)
• respiratory system phenotype
• abnormal pulmonary alveolar duct morphology
  • enlarged air spaces distal to the terminal bronchioles   (MGI Ref ID J:114985)
• abnormal pulmonary alveolus morphology
  • destruction of normal alveolar structures   (MGI Ref ID J:114985)
• enlarged lung
  • significantly increased lung volume at 3 months of age in contrast to normal body weights through 12 months   (MGI Ref ID J:114985)
• skeleton phenotype
• increased bone mass
  • larger bones at 20-24 weeks of age   (MGI Ref ID J:118467)
• increased bone mineral content
  • greater mineral content at 20-24 weeks   (MGI Ref ID J:118467)
• adipose tissue phenotype
• decreased percent body fat
  • 70% less body fat   (MGI Ref ID J:118467)
• vision/eye phenotype
• abnormal retinal layer morphology   (MGI Ref ID J:141070)
• • abnormal retinal pigment epithelium morphology
    • increased number of proliferating cells in the retinal pigment cell layer at 6 days of age   (MGI Ref ID J:141070)
  • abnormal retinal rod cell morphology
    • increased differentiation of rod photoreceptors at around 13 days of age   (MGI Ref ID J:141070)
    • growth factor treatment enhances proliferation   (MGI Ref ID J:141070)
• abnormal retinal progenitor cell morphology
  • more proliferating cells in the retina at 6 days of age   (MGI Ref ID J:141070)
  • neuronal differentiation of precursors   (MGI Ref ID J:141070)
  • FGF-2 injection is required for a similar effect after 15 days of age   (MGI Ref ID J:141070)
• abnormal retinal rod bipolar cell morphology
  • increased differentiation at around 13 days of age   (MGI Ref ID J:141070)
  • growth factor treatment enhances proliferation   (MGI Ref ID J:141070)
• nervous system phenotype
• abnormal retinal rod bipolar cell morphology
  • increased differentiation at around 13 days of age   (MGI Ref ID J:141070)
  • growth factor treatment enhances proliferation   (MGI Ref ID J:141070)
• abnormal retinal rod cell morphology
  • increased differentiation of rod photoreceptors at around 13 days of age   (MGI Ref ID J:141070)
  • growth factor treatment enhances proliferation   (MGI Ref ID J:141070)
• pigmentation phenotype
• abnormal retinal pigment epithelium morphology
  • increased number of proliferating cells in the retinal pigment cell layer at 6 days of age   (MGI Ref ID J:141070)
• hematopoietic system phenotype
• increased spleen weight
  • spleen weight elevated with dextran sodium sulfate treatment relative to controls   (MGI Ref ID J:37271)
• cardiovascular system phenotype
• rectal hemorrhage
  • rectal bleeding after 7 days of dextran sodium sulfate treatment is reduced relative to controls   (MGI Ref ID J:37271)

Tlr4^lps-del/Tlr4^lps-del

        involves: C57BL/10ScN

• immune system phenotype
• abnormal tumor necrosis factor level
  • diminished TNF alpha expression after 90 minutes of liver ischemia followed by 6 hours of reperfusion   (MGI Ref ID J:114368)
• decreased interleukin-10 secretion
  • production is suppressed in response to E coli with or without adenosine   (MGI Ref ID J:147023)
• decreased interleukin-6 secretion
  • production is suppressed in response to E coli   (MGI Ref ID J:147023)
• homeostasis/metabolism phenotype
• abnormal circulating enzyme level
  • myeloperoxidase levels are reduced after 90 minutes of liver ischemia followed by 6 hours of reperfusion   (MGI Ref ID J:114368)
  • increased HO-1 expression   (MGI Ref ID J:114368)
• • decreased circulating alanine transaminase level
    • serum levels decreased after 90 minutes of liver ischemia followed by 6 hours of reperfusion   (MGI Ref ID J:114368)
• abnormal tumor necrosis factor level
  • diminished TNF alpha expression after 90 minutes of liver ischemia followed by 6 hours of reperfusion   (MGI Ref ID J:114368)
• nervous system phenotype
• CNS ischemia
  • improved neurological score relative to controls after ischemia/reperfusion   (MGI Ref ID J:148091)
  • reduced infarct size relative to controls at 24 hours after ischemia/reperfusion   (MGI Ref ID J:148091)
• abnormal neuron differentiation
  • improved differentiation of progenitor cells into neurons   (MGI Ref ID J:129957)
  • survival of newly formed neurons is reduced   (MGI Ref ID J:129957)
• cellular phenotype
• abnormal neuron differentiation
  • improved differentiation of progenitor cells into neurons   (MGI Ref ID J:129957)
  • survival of newly formed neurons is reduced   (MGI Ref ID J:129957)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
      Tlr deficiency
Immunodeficiency
      Tlr deficiency
Inflammation
      Tlr deficiency

Tlr4^lps-del related

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
      Tlr deficiency
Immunodeficiency
      Tlr deficiency
Inflammation
      Tlr deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Tlr4lps-del
Allele Name		defective lipopolysaccharide response, deletion
Allele Type		Spontaneous
Common Name(s)		TLR4-KO; TLR4lps-; Tlr4-;
Strain of Origin		C57BL/10ScN
Gene Symbol and Name		Tlr4, toll-like receptor 4
Chromosome		4
Gene Common Name(s)		ARMD10; CD284; Lps; RAS-like, family 2, locus 8; Rasl2-8; TLR-4; TOLL; lipopolysaccharide response;
General Note		C57BL/10ScN, C57BL/10ScNJ, and C57BL10/ScCr mice carry this allele.
Molecular Note		This allele has been characterized by lack of mRNA owing to a deletion of the locus. 74723bp of genomic DNA sequence have been removed, apparently encompasing only the Tlr4 gene. [MGI Ref ID J:122203] [MGI Ref ID J:51522]

Genotyping

Genotyping Information

Genotyping Protocols

Tlr4^lps-del, Melt Curve Analysis
Tlr4^lps-del, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Poltorak A; He X; Smirnova I; Liu MY; Huffel CV; Du X; Birdwell D; Alejos E; Silva M; Galanos C; Freudenberg M; Ricciardi-Castagnoli P; Layton B; Beutler B. 1998. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science 282(5396):2085-8. [PubMed: 9851930]  [MGI Ref ID J:51522]

Vogel SN; Hansen CT; Rosenstreich DL. 1979. Characterization of a congenitally LPS-resistant, athymic mouse strain. J Immunol 122(2):619-22. [PubMed: 368244]  [MGI Ref ID J:6086]

Additional References

Tlr4^lps-del related

Aachoui Y; Leaf IA; Hagar JA; Fontana MF; Campos CG; Zak DE; Tan MH; Cotter PA; Vance RE; Aderem A; Miao EA. 2013. Caspase-11 protects against bacteria that escape the vacuole. Science 339(6122):975-8. [PubMed: 23348507]  [MGI Ref ID J:193387]

Agace W; Hedges S; Svanborg C. 1992. Lps genotype in the C57 black mouse background and its influence on the interleukin-6 response to E. coli urinary tract infection. Scand J Immunol 35(5):531-8. [PubMed: 1579857]  [MGI Ref ID J:3436]

Amiel E; Alonso A; Uematsu S; Akira S; Poynter ME; Berwin B. 2009. Pivotal Advance: Toll-like receptor regulation of scavenger receptor-A-mediated phagocytosis. J Leukoc Biol 85(4):595-605. [PubMed: 19112093]  [MGI Ref ID J:146918]

An CH; Wang XM; Lam HC; Ifedigbo E; Washko GR; Ryter SW; Choi AM. 2012. TLR4 deficiency promotes autophagy during cigarette smoke-induced pulmonary emphysema. Am J Physiol Lung Cell Mol Physiol 303(9):L748-57. [PubMed: 22983353]  [MGI Ref ID J:193661]

Andonegui G; Kerfoot SM; McNagny K; Ebbert KV; Patel KD; Kubes P. 2005. Platelets express functional Toll-like receptor-4. Blood 106(7):2417-23. [PubMed: 15961512]  [MGI Ref ID J:119375]

Babelova A; Moreth K; Tsalastra-Greul W; Zeng-Brouwers J; Eickelberg O; Young MF; Bruckner P; Pfeilschifter J; Schaefer RM; Grone HJ; Schaefer L. 2009. Biglycan, a danger signal that activates the NLRP3 inflammasome via toll-like and P2X receptors. J Biol Chem 284(36):24035-48. [PubMed: 19605353]  [MGI Ref ID J:155256]

Beisswenger C; Lysenko ES; Weiser JN. 2009. Early bacterial colonization induces toll-like receptor-dependent transforming growth factor beta signaling in the epithelium. Infect Immun 77(5):2212-20. [PubMed: 19255194]  [MGI Ref ID J:148184]

Benabid R; Wartelle J; Malleret L; Guyot N; Gangloff S; Lebargy F; Belaaouaj A. 2012. Neutrophil elastase modulates cytokine expression: contribution to host defense against Pseudomonas aeruginosa-induced pneumonia. J Biol Chem 287(42):34883-94. [PubMed: 22927440]  [MGI Ref ID J:192139]

Berger SB; Romero X; Ma C; Wang G; Faubion WA; Liao G; Compeer E; Keszei M; Rameh L; Wang N; Boes M; Regueiro JR; Reinecker HC; Terhorst C. 2010. SLAM is a microbial sensor that regulates bacterial phagosome functions in macrophages. Nat Immunol 11(10):920-7. [PubMed: 20818396]  [MGI Ref ID J:164685]

Bhattacharyya S; Dudeja PK; Tobacman JK. 2008. Lipopolysaccharide activates NF-kappaB by TLR4-Bcl10-dependent and independent pathways in colonic epithelial cells. Am J Physiol Gastrointest Liver Physiol 295(4):G784-90. [PubMed: 18718996]  [MGI Ref ID J:142282]

Binck BW; Tsen MF; Islas M; White DJ; Schultz RA; Willis MS; Garcia JV; Horton JW; Thomas JA. 2005. Bone marrow-derived cells contribute to contractile dysfunction in endotoxic shock. Am J Physiol Heart Circ Physiol 288(2):H577-83. [PubMed: 15458952]  [MGI Ref ID J:96165]

Biragyn A; Coscia M; Nagashima K; Sanford M; Young HA; Olkhanud P. 2008. Murine beta-defensin 2 promotes TLR-4/MyD88-mediated and NF-kappaB-dependent atypical death of APCs via activation of TNFR2. J Leukoc Biol 83(4):998-1008. [PubMed: 18192488]  [MGI Ref ID J:134193]

Bosmann M; Haggadone MD; Hemmila MR; Zetoune FS; Sarma JV; Ward PA. 2012. Complement activation product C5a is a selective suppressor of TLR4-induced, but not TLR3-induced, production of IL-27(p28) from macrophages. J Immunol 188(10):5086-93. [PubMed: 22491257]  [MGI Ref ID J:188678]

Burch LH; Yang IV; Whitehead GS; Chao FG; Berman KG; Schwartz DA. 2006. The transcriptional response to lipopolysaccharide reveals a role for interferon-gamma in lung neutrophil recruitment. Am J Physiol Lung Cell Mol Physiol 291(4):L677-82. [PubMed: 16766576]  [MGI Ref ID J:144404]

Burns E; Bachrach G; Shapira L; Nussbaum G. 2006. Cutting Edge: TLR2 is required for the innate response to Porphyromonas gingivalis: activation leads to bacterial persistence and TLR2 deficiency attenuates induced alveolar bone resorption. J Immunol 177(12):8296-300. [PubMed: 17142724]  [MGI Ref ID J:140676]

Carpentier PA; Dingman AL; Palmer TD. 2011. Placental TNF-alpha Signaling in Illness-Induced Complications of Pregnancy. Am J Pathol 178(6):2802-10. [PubMed: 21641402]  [MGI Ref ID J:173471]

Caso JR; Pradillo JM; Hurtado O; Lorenzo P; Moro MA; Lizasoain I. 2007. Toll-like receptor 4 is involved in brain damage and inflammation after experimental stroke. Circulation 115(12):1599-608. [PubMed: 17372179]  [MGI Ref ID J:133065]

Chabot S; Wagner JS; Farrant S; Neutra MR. 2006. TLRs regulate the gatekeeping functions of the intestinal follicle-associated epithelium. J Immunol 176(7):4275-83. [PubMed: 16547265]  [MGI Ref ID J:129895]

Chan YR; Liu JS; Pociask DA; Zheng M; Mietzner TA; Berger T; Mak TW; Clifton MC; Strong RK; Ray P; Kolls JK. 2009. Lipocalin 2 is required for pulmonary host defense against Klebsiella infection. J Immunol 182(8):4947-56. [PubMed: 19342674]  [MGI Ref ID J:147498]

Chen J; Hartono JR; John R; Bennett M; Zhou XJ; Wang Y; Wu Q; Winterberg PD; Nagami GT; Lu CY. 2011. Early interleukin 6 production by leukocytes during ischemic acute kidney injury is regulated by TLR4. Kidney Int 80(5):504-15. [PubMed: 21633411]  [MGI Ref ID J:194804]

Chen J; John R; Richardson JA; Shelton JM; Zhou XJ; Wang Y; Wu QQ; Hartono JR; Winterberg PD; Lu CY. 2011. Toll-like receptor 4 regulates early endothelial activation during ischemic acute kidney injury. Kidney Int 79(3):288-99. [PubMed: 20927041]  [MGI Ref ID J:186890]

Cohen-Sfady M; Pevsner-Fischer M; Margalit R; Cohen IR. 2009. Heat shock protein 60, via MyD88 innate signaling, protects B cells from apoptosis, spontaneous and induced. J Immunol 183(2):890-6. [PubMed: 19561102]  [MGI Ref ID J:151657]

Costalonga M; Zell T. 2007. Lipopolysaccharide enhances in vivo interleukin-2 production and proliferation by naive antigen-specific CD4 T cells via a Toll-like receptor 4-dependent mechanism. Immunology 122(1):124-30. [PubMed: 17484770]  [MGI Ref ID J:125617]

Csoka B; Nemeth ZH; Virag L; Gergely P; Leibovich SJ; Pacher P; Sun CX; Blackburn MR; Vizi ES; Deitch EA; Hasko G. 2007. A2A adenosine receptors and C/EBPbeta are crucially required for IL-10 production by macrophages exposed to Escherichia coli. Blood 110(7):2685-95. [PubMed: 17525287]  [MGI Ref ID J:147023]

D'Avila H; Almeida PE; Roque NR; Castro-Faria-Neto HC; Bozza PT. 2007. Toll-Like Receptor-2-Mediated C-C Chemokine Receptor 3 and Eotaxin-Driven Eosinophil Influx Induced by Mycobacterium bovis BCG Pleurisy. Infect Immun 75(3):1507-11. [PubMed: 17158890]  [MGI Ref ID J:118500]

D'Avila H; Melo RC; Parreira GG; Werneck-Barroso E; Castro-Faria-Neto HC; Bozza PT. 2006. Mycobacterium bovis bacillus Calmette-Guerin induces TLR2-mediated formation of lipid bodies: intracellular domains for eicosanoid synthesis in vivo. J Immunol 176(5):3087-97. [PubMed: 16493068]  [MGI Ref ID J:129415]

Darville T; O'Neill JM; Andrews CW Jr; Nagarajan UM; Stahl L; Ojcius DM. 2003. Toll-like receptor-2, but not Toll-like receptor-4, is essential for development of oviduct pathology in chlamydial genital tract infection. J Immunol 171(11):6187-97. [PubMed: 14634135]  [MGI Ref ID J:106730]

David MD; Cochrane CL; Duncan SK; Schrader JW. 2005. Pure lipopolysaccharide or synthetic lipid A induces activation of p21Ras in primary macrophages through a pathway dependent on Src family kinases and PI3K. J Immunol 175(12):8236-41. [PubMed: 16339563]  [MGI Ref ID J:122258]

Davis MJ; Gregorka B; Gestwicki JE; Swanson JA. 2012. Inducible renitence limits Listeria monocytogenes escape from vacuoles in macrophages. J Immunol 189(9):4488-95. [PubMed: 23002437]  [MGI Ref ID J:190625]

Desai MS; Mariscalco MM; Tawil A; Vallejo JG; Smith CW. 2008. Atherogenic diet-induced hepatitis is partially dependent on murine TLR4. J Leukoc Biol 83(6):1336-44. [PubMed: 18334542]  [MGI Ref ID J:136848]

Dong B; Qi D; Yang L; Huang Y; Xiao X; Tai N; Wen L; Wong FS. 2012. TLR4 regulates cardiac lipid accumulation and diabetic heart disease in the nonobese diabetic mouse model of type 1 diabetes. Am J Physiol Heart Circ Physiol 303(6):H732-42. [PubMed: 22842069]  [MGI Ref ID J:191323]

Dvoriantchikova G; Barakat DJ; Hernandez E; Shestopalov VI; Ivanov D. 2010. Toll-like receptor 4 contributes to retinal ischemia/reperfusion injury. Mol Vis 16:1907-12. [PubMed: 21031135]  [MGI Ref ID J:167913]

Ehl S; Bischoff R; Ostler T; Vallbracht S; Schulte-Monting J; Poltorak A; Freudenberg M. 2004. The role of Toll-like receptor 4 versus interleukin-12 in immunity to respiratory syncytial virus. Eur J Immunol 34(4):1146-53. [PubMed: 15048726]  [MGI Ref ID J:88878]

Espinassous Q; Garcia-de-Paco E; Garcia-Verdugo I; Synguelakis M; von Aulock S; Sallenave JM; McKenzie AN; Kanellopoulos J. 2009. IL-33 enhances lipopolysaccharide-induced inflammatory cytokine production from mouse macrophages by regulating lipopolysaccharide receptor complex. J Immunol 183(2):1446-55. [PubMed: 19553541]  [MGI Ref ID J:151399]

Fan W; Morinaga H; Kim JJ; Bae E; Spann NJ; Heinz S; Glass CK; Olefsky JM. 2010. FoxO1 regulates Tlr4 inflammatory pathway signalling in macrophages. EMBO J 29(24):4223-36. [PubMed: 21045807]  [MGI Ref ID J:167177]

Fellner L; Irschick R; Schanda K; Reindl M; Klimaschewski L; Poewe W; Wenning GK; Stefanova N. 2013. Toll-like receptor 4 is required for alpha-synuclein dependent activation of microglia and astroglia. Glia 61(3):349-60. [PubMed: 23108585]  [MGI Ref ID J:191118]

Feng X; Deng T; Zhang Y; Su S; Wei C; Han D. 2011. Lipopolysaccharide inhibits macrophage phagocytosis of apoptotic neutrophils by regulating the production of tumour necrosis factor alpha and growth arrest-specific gene 6. Immunology 132(2):287-95. [PubMed: 21039473]  [MGI Ref ID J:168633]

Ganta RR; Wilkerson MJ; Cheng C; Rokey AM; Chapes SK. 2002. Persistent Ehrlichia chaffeensis infection occurs in the absence of functional major histocompatibility complex class II genes. Infect Immun 70(1):380-8. [PubMed: 11748204]  [MGI Ref ID J:74569]

Good DW; George T; Watts BA 3rd. 2012. Toll-like receptor 2 is required for LPS-induced Toll-like receptor 4 signaling and inhibition of ion transport in renal thick ascending limb. J Biol Chem 287(24):20208-20. [PubMed: 22523073]  [MGI Ref ID J:186513]

Goodyear A; Troyer R; Bielefeldt-Ohmann H; Dow S. 2012. MyD88-Dependent Recruitment of Monocytes and Dendritic Cells Required for Protection from Pulmonary Burkholderia mallei Infection. Infect Immun 80(1):110-20. [PubMed: 22025508]  [MGI Ref ID J:178961]

Ha T; Li Y; Hua F; Ma J; Gao X; Kelley J; Zhao A; Haddad GE; Williams DL; William Browder I; Kao RL; Li C. 2005. Reduced cardiac hypertrophy in toll-like receptor 4-deficient mice following pressure overload. Cardiovasc Res 68(2):224-34. [PubMed: 15967420]  [MGI Ref ID J:106358]

Haziot A; Hijiya N; Gangloff SC; Silver J; Goyert SM. 2001. Induction of a novel mechanism of accelerated bacterial clearance by lipopolysaccharide in CD14-deficient and Toll-like receptor 4-deficient mice. J Immunol 166(2):1075-8. [PubMed: 11145687]  [MGI Ref ID J:127072]

He RL; Zhou J; Hanson CZ; Chen J; Cheng N; Ye RD. 2009. Serum amyloid A induces G-CSF expression and neutrophilia via Toll-like receptor 2. Blood 113(2):429-37. [PubMed: 18952897]  [MGI Ref ID J:144859]

He S; Liang Y; Shao F; Wang X. 2011. Toll-like receptors activate programmed necrosis in macrophages through a receptor-interacting kinase-3-mediated pathway. Proc Natl Acad Sci U S A 108(50):20054-9. [PubMed: 22123964]  [MGI Ref ID J:180434]

Heimesaat MM; Fischer A; Jahn HK; Niebergall J; Freudenberg M; Blaut M; Liesenfeld O; Schumann RR; Gobel UB; Bereswill S. 2007. Exacerbation of murine ileitis by Toll-like receptor 4 mediated sensing of lipopolysaccharide from commensal Escherichia coli. Gut 56(7):941-8. [PubMed: 17255219]  [MGI Ref ID J:131231]

Heimesaat MM; Fischer A; Siegmund B; Kupz A; Niebergall J; Fuchs D; Jahn HK; Freudenberg M; Loddenkemper C; Batra A; Lehr HA; Liesenfeld O; Blaut M; Gobel UB; Schumann RR; Bereswill S. 2007. Shift towards pro-inflammatory intestinal bacteria aggravates acute murine colitis via Toll-like receptors 2 and 4. PLoS ONE 2(7):e662. [PubMed: 17653282]  [MGI Ref ID J:129318]

Henao-Mejia J; Elinav E; Jin C; Hao L; Mehal WZ; Strowig T; Thaiss CA; Kau AL; Eisenbarth SC; Jurczak MJ; Camporez JP; Shulman GI; Gordon JI; Hoffman HM; Flavell RA. 2012. Inflammasome-mediated dysbiosis regulates progression of NAFLD and obesity. Nature 482(7384):179-85. [PubMed: 22297845]  [MGI Ref ID J:181354]

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Tarallo V; Hirano Y; Gelfand BD; Dridi S; Kerur N; Kim Y; Cho WG; Kaneko H; Fowler BJ; Bogdanovich S; Albuquerque RJ; Hauswirth WW; Chiodo VA; Kugel JF; Goodrich JA; Ponicsan SL; Chaudhuri G; Murphy MP; Dunaief JL; Ambati BK; Ogura Y; Yoo JW; Lee DK; Provost P; Hinton DR; Nunez G; Baffi JZ; Kleinman ME; Ambati J. 2012. DICER1 loss and Alu RNA induce age-related macular degeneration via the NLRP3 inflammasome and MyD88. Cell 149(4):847-59. [PubMed: 22541070]  [MGI Ref ID J:186198]

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Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred as homozygotes.
Mating System	Homozygote x Homozygote         (Female x Male)   28-APR-09
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• View the complete collection of spontaneous mutants in the Mouse Mutant Resource.
• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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