Strain Name:

B6.Cg-Tg(Alb-PLG)1Dgi/J

Stock Number:

007238

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
The level of PLG in the plasma of these mice expressing the human plasminogen gene under the control of a mouse albumin promoter is approximately 16% of the level in control human plasma. Mice exhibit increased susceptibility to group A streptococci (GAS). Although enhanced green fluorescent protein (EGFP) is incorporated in the transgene, it is not expressed.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6(SJL)-Tg(Alb-PLG)1Dgi/J    (Changed: 16-MAY-07 )
Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationN10pN1
Generation Definitions
 
Donating Investigator David Ginsburg,   University of Michigan

Description
The level of PLG in the plasma of these mice expressing the human plasminogen gene under the control of a mouse albumin promoter is approximately 16% of the level in control human plasma. Mice exhibit increased susceptibility to group A streptococci (GAS). Although enhanced green fluorescent protein (EGFP) is incorporated in the transgene, it is not expressed.

Development
A transgenic BAC was designed to place human plasminogen (PLG) and green fluorescent protein (EGFP) under the control of a mouse albumin promoter (Alb). A chimeric intron with optimized splice signals from the pCI expression vector was placed upstream of the PLG cDNA. An FRT site and PGK-Tn5-Neo cassette were placed downstream of EGFP. The whole region, with the exception of Alb sequences, was flanked by loxP sites oriented in opposite directions. The PLG BAC DNA was isolated and microinjected into fertilized oocytes. Pronuclear fertilized eggs obtained by mating (C57BL6/J x SJL/J)F1 or C57BL/6 female mice with (C57BL6/J x SJL/J) F1 male mice were used for injection. This line has been backcrossed to C57BL/6 fifteen of more times by the donating laboratory. EGFP expression is not seen.

Related Strains

View Strains carrying other alleles of Alb     (9 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested.
Plasminogen Deficiency, Type I   (PLG)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Tg(Alb-PLG)1Dgi/0

        involves: C57BL/6 * C57BL/6J * SJL
  • mortality/aging
  • increased sensitivity to induced morbidity/mortality
    • mice infected subcutaneously with the human pathogenic group A streptococcus (GAS) strain UMAA2616 exhibit 75% of mortality unlike similarly treated wild-type mice that exhibit 20% mortality   (MGI Ref ID J:120780)
    • mice infected subcutaneously with GAS strain MGAS166 exhibit 35% to 100% of mortality unlike similarly treated wild-type mice that exhibit 30% mortality   (MGI Ref ID J:120780)
    • however, mice exhibit normal mortality when infected with GAS strain UMAA2641, which lacks the Ska gene, or when infected intravenously with UMAA2616   (MGI Ref ID J:120780)
    • mice infected subcutaneously with PAM-expressing GAS strain AP53 exhibit 80% mortality compared to 20% in similarly treated wild-type mice   (MGI Ref ID J:120780)
  • immune system phenotype
  • increased susceptibility to bacterial infection
    • mice infected subcutaneously with the human pathogenic group A streptococcus (GAS) strain UMAA2616 exhibit 75% of mortality unlike similarly treated wild-type mice that exhibit 20% mortality   (MGI Ref ID J:120780)
    • mice infected subcutaneously with GAS strain MGAS166 exhibit 35% to 100% of mortality unlike similarly treated wild-type mice that exhibit 30% mortality   (MGI Ref ID J:120780)
    • however, mice exhibit normal mortality when infected with GAS strain UMAA2641, which lacks the Ska gene, or when infected intravenously with UMAA2616   (MGI Ref ID J:120780)
    • mice infected subcutaneously with PAM-expressing GAS strain AP53 exhibit 80% mortality compared to 20% in similarly treated wild-type mice   (MGI Ref ID J:120780)
    • mice infected subcutaneously with GAS exhibit increased bacterial colonies in the spleen compared to similarly infected wild-type mice   (MGI Ref ID J:120780)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Hematological Research
Clotting Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Alb-PLG)1Dgi
Allele Name transgene insertion 1, David Ginsburg
Allele Type Transgenic (Inserted expressed sequence)
Strain of Origin(C57BL/6J x SJL/J)F1 x (C57BL/6J x SJL/J)F1 or (C57BL/6 x (C57BL/6J x SJL/J)F1)
Expressed Gene PLG, plasminogen, human
Promoter Alb, albumin, mouse, laboratory
Molecular Note A cassette containing the human PLG cDNA from nucleotide -13 (A of ATG initiation codon as +1) to +2476 (including the entire coding sequence) was followed by the green fluorescent protein (EGFP) coding sequence, preceded by an internal ribosome entry site sequence (IRES) and a bacterial kanamycin-resistant gene (KAN). A chimeric intron with optimized splice signals from the pCI expression vector was placed upstream of the PLG cDNA. Homologous recombination between the transgene targeting construct and the murine Alb BAC was carried out in E. coli, replacing the murine Alb gene (from nucleotide -12 to exon 12) by the transgene cassette. EGFP expression is not detected. [MGI Ref ID J:120780]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Alb-PLG)1Dgi, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Sun H; Ringdahl U; Homeister JW; Fay WP; Engleberg NC; Yang AY; Rozek LS; Wang X; Sjobring U; Ginsburg D. 2004. Plasminogen is a critical host pathogenicity factor for group A streptococcal infection. Science 305(5688):1283-6. [PubMed: 15333838]  [MGI Ref ID J:120780]

Additional References

Tg(Alb-PLG)1Dgi related

Sun H; Wang X; Degen JL; Ginsburg D. 2009. Reduced thrombin generation increases host susceptibility to group A streptococcal infection. Blood 113(6):1358-64. [PubMed: 19056689]  [MGI Ref ID J:145540]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.8)