Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6(SJL)-Tg(Alb-PLG)1Dgi/J    (Changed: 16-MAY-07 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N10pN1   Donating Investigator David Ginsburg,   University of Michigan

Description
The level of PLG in the plasma of these mice expressing the human plasminogen gene under the control of a mouse albumin promoter is approximately 16% of the level in control human plasma. Mice exhibit increased susceptibility to group A streptococci (GAS). Although enhanced green fluorescent protein (EGFP) is incorporated in the transgene, it is not expressed.

Development
A transgenic BAC was designed to place human plasminogen (PLG) and green fluorescent protein (EGFP) under the control of a mouse albumin promoter (Alb). A chimeric intron with optimized splice signals from the pCI expression vector was placed upstream of the PLG cDNA. An FRT site and PGK-Tn5-Neo cassette were placed downstream of EGFP. The whole region, with the exception of Alb sequences, was flanked by loxP sites oriented in opposite directions. The PLG BAC DNA was isolated and microinjected into fertilized oocytes. Pronuclear fertilized eggs obtained by mating (C57BL6/J x SJL/J)F1 or C57BL/6 female mice with (C57BL6/J x SJL/J) F1 male mice were used for injection. This line has been backcrossed to C57BL/6 fifteen of more times by the donating laboratory. EGFP expression is not seen.

Related Strains

Strains carrying other alleles of Alb

007853	129S/SvEv-Tg(Alb1-Ren)1Unc/CofJ
000186	B6.Cg-Albc/J
007244	B6;SJL-Tg(Alb-F5)2Dgi/J
007245	B6;SJL-Tg(Alb-F5)3Dgi/J
002226	C57BL/6J-Tg(Alb1HBV)44Bri/J

View Strains carrying other alleles of Alb     (5 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tg(Alb-PLG)1Dgi/0

        involves: C57BL/6 * C57BL/6J * SJL

• life span-post-weaning/aging
• increased sensitivity to induced morbidity/mortality (MGI Ref ID J:120780)
  • mice infected subcutaneously with the human pathogenic group A streptococcus (GAS) strain UMAA2616 exhibit 75% of mortality unlike similarly treated wild-type mice that exhibit 20% mortality
  • mice infected subcutaneously with GAS strain MGAS166 exhibit 35% to 100% of mortality unlike similarly treated wild-type mice that exhibit 30% mortality
  • however, mice exhibit normal mortality when infected with GAS strain UMAA2641, which lacks the Ska gene, or when infected intravenously with UMAA2616
  • mice infected subcutaneously with PAM-expressing GAS strain AP53 exhibit 80% mortality compared to 20% in similarly treated wild-type mice
• immune system phenotype
• increased susceptibility to bacterial infection (MGI Ref ID J:120780)
  • mice infected subcutaneously with the human pathogenic group A streptococcus (GAS) strain UMAA2616 exhibit 75% of mortality unlike similarly treated wild-type mice that exhibit 20% mortality
  • mice infected subcutaneously with GAS strain MGAS166 exhibit 35% to 100% of mortality unlike similarly treated wild-type mice that exhibit 30% mortality
  • however, mice exhibit normal mortality when infected with GAS strain UMAA2641, which lacks the Ska gene, or when infected intravenously with UMAA2616
  • mice infected subcutaneously with PAM-expressing GAS strain AP53 exhibit 80% mortality compared to 20% in similarly treated wild-type mice
  • mice infected subcutaneously with GAS exhibit increased bacterial colonies in the spleen compared to similarly infected wild-type mice

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Hematological Research
Clotting Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(Alb-PLG)1Dgi
Allele Name		transgene insertion 1, David Ginsburg
Allele Type		Transgenic (random, expressed)
Strain of Origin		(C57BL6/J x SJL/J)F1 X (C57BL6/J x SJL/J)F1 or C57BL/6 X (C57BL6/J x SJL/J)F1
Expressed Gene		PLG, plasminogen, human
Promoter		Alb, albumin, mouse, laboratory
Molecular Note		A cassette containing the human PLG cDNA from nucleotide -13 (A of ATG initiation codon as +1) to +2476 (including the entire coding sequence) was followed by the green fluorescent protein (EGFP) coding sequence, preceded by an internal ribosome entry site sequence (IRES) and a bacterial kanamycin-resistant gene (KAN). A chimeric intron with optimized splice signals from the pCI expression vector was placed upstream of the PLG cDNA. Homologous recombination between the transgene targeting construct and the murine Alb BAC was carried out in E. coli, replacing the murine Alb gene (from nucleotide -12 to exon 12) by the transgene cassette. EGFP expression is not detected. [MGI Ref ID J:120780]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Alb-PLG)1Dgi, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Sun H; Ringdahl U; Homeister JW; Fay WP; Engleberg NC; Yang AY; Rozek LS; Wang X; Sjobring U; Ginsburg D. 2004. Plasminogen is a critical host pathogenicity factor for group A streptococcal infection. Science 305(5688):1283-6. [PubMed: 15333838]  [MGI Ref ID J:120780]

Additional References

Tg(Alb-PLG)1Dgi related

Sun H; Wang X; Degen JL; Ginsburg D. 2009. Reduced thrombin generation increases host susceptibility to group A streptococcal infection. Blood 113(6):1358-64. [PubMed: 19056689]  [MGI Ref ID J:145540]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). This strain is included in the Induced Mutant Resource Colony collection.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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