Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.Cg-Tg(Alb-F5)2Dgi/J    (Changed: 10-DEC-07 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse   Donating Investigator David Ginsburg,   University of Michigan

Description
Liver-specific expression of the transgene is exhibited in this strain of mice as determined by RT-PCR. When crossed with a F5 targeted mouse (Stock No. 004078), homozygous knockout/hemizygous transgenic mice are grossly normal and show no evidence of spontaneous hemorrhage; compound mutants have a plasma F5 level that is ~45% that of wildtype mice, no platelet F5, slightly prolonged bleeding times, and show transgene rescue of both mid-embryonic lethality and perinatal hemorrhage phenotypes associated with F5 knockouts.

Development
A mouse Alb (albumin) promoter drives expression of the mouse F5 (FV) transgene including the entire coding sequence. An internal ribosome entry site sequence (IRES) followed by an enhanced green fluorescent protein (EGFP) coding sequence and a bacterial kanamycin resistant gene (KAN) were also built into the cassette. A chimeric intron with optimized splice signals from the pCI expression vector was also introduced upstream of the F5 cDNA in the transgene cassette to enhance gene expression. No EGFP expression is observed. The vector was injected into fertilized oocytes created by mating B6SJLF1 or C57BL/6 female mice with B6SJLF1 male mice. This line has been backcrossed minimally (perhaps twice) to C57BL/6 by the donating laboratory.

Related Strains

Strains carrying other alleles of Alb

007853	129S/SvEv-Tg(Alb1-Ren)1Unc/CofJ
000186	B6.Cg-Albc/J
007238	B6.Cg-Tg(Alb-PLG)1Dgi/J
007245	B6;SJL-Tg(Alb-F5)3Dgi/J
002226	C57BL/6J-Tg(Alb1HBV)44Bri/J

View Strains carrying other alleles of Alb     (5 strains)

Strains carrying other alleles of F5

004078	B6.129S2-F5tm1Dgi/J
004080	B6.129S2-F5tm2Dgi/J
007245	B6;SJL-Tg(Alb-F5)3Dgi/J

View Strains carrying other alleles of F5     (3 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research

Hematological Research
Clotting Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(Alb-F5)2Dgi
Allele Name		transgene insertion 2, David Ginsburg
Allele Type		Transgenic (random, expressed)
Common Name(s)		AlbfvC;
Mutation Made By		David Ginsburg,   University of Michigan
Strain of Origin		(C57BL/6 x SJL)F1 or C57BL/6 x (C57BL/6 x SJL)F1
Expressed Gene		F5, coagulation factor V, mouse, laboratory
Promoter		Alb, albumin, mouse, laboratory
Molecular Note		The transgenic construct has the mouse albumin promoter driving expression of the murine F5 (FV) gene including the entire coding sequence. An IRES-EGFP cassette with a bacterial gene wasinserting after the F5 coding sequence. A chimeric intron with optimized splice signals was introduced upstream of the F5 cDNA enhance gene expression. Transgenic F5 expression is restricted to the liver. EGFP expression is not detected. [MGI Ref ID J:86150]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Cxc14-F5)1Dgi, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Sun H; Yang TL; Yang A; Wang X; Ginsburg D. 2003. The murine platelet and plasma factor V pools are biosynthetically distinct and sufficient for minimal hemostasis. Blood 102(8):2856-61. [PubMed: 12855561]  [MGI Ref ID J:86150]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). This strain is included in the Induced Mutant Resource Colony collection.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

Terms of Use

General Terms and Conditions

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Contact information

General inquiries

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