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| This strain functions as a reporter strain for Smn1 (survival motor neuron 1) in the heterozygous state. Exons 1 through 8 of the mouse Smn1 gene (12.8 kb) were replaced with lacZ and an FRT site remaining from the deletion of a selection marker. This mutant mouse strain may be useful in studies of Spinal Muscular Atrophy. | |||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Donating Investigator Andrew Murphy, Regeneron Pharmaceuticals, Inc. Description
This strain functions as a reporter strain for Smn1 (survival motor neuron 1) in the heterozygous state. Exons 1 through 8 of the mouse Smn1 gene (12.8 kb) were replaced with lacZ and an FRT site remaining from the deletion of a selection marker. This allele is a functional null and homozygous animals are embryonic lethal.Importation of this model was supported by the Spinal Muscular Atrophy Foundation.
Development
Exons 1 through 8 of the mouse Smn1 (survival motor neuron 1) gene (12.8 kb) were replaced with lacZ and an FRT site remaining from the deletion of a selection marker. 129S6/SvEvTac and C57BL/6Tac derived F1H4 ES cells were used. This strain was maintained on a B6;129 genetic background. This allele is also being backcrossed to both FVB/NJ and C57BL/6J.
Related Strains
Strains carrying other alleles of Smn1
View Strains carrying other alleles of Smn1 (16 strains)
Phenotype
Phenotype Information
assigned by genotype
Smn1tm2Mrph/Smn1tm2Mrph
involves: 129S6/SvEvTac * C57BL/6NTac
- lethality-prenatal/perinatal
- prenatal lethality (MGI Ref ID J:135422)
- homozygotes die during development
This mouse can be used to support research in many areas including:
Developmental Biology Research
Embryonic Lethality (Homozygous)
Neurobiology Research
Neurodegeneration
Spinal Muscular Atrophy (SMA)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Smn1tm2Mrph | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Andrew Murphy | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Smn1KI; Smn1KO; Smn1lacZ; | ||
| Strain of Origin | (129 x C57BL/6)F1 | ||
| ES Cell Line Name | F1H4 | ||
| ES Cell Line Strain | (129 x C57BL/6)F1 | ||
| Gene Symbol and Name | Smn1, survival motor neuron 1 | ||
| Chromosome | 13 | ||
| Gene Common Name(s) | AI849087; BCD541; SMA; SMA1; SMA2; SMA3; SMA4; SMA@; SMN; SMNT; Smn; T-BCD541; expressed sequence AI849087; survival motor neuron; | ||
| Molecular Note | Exons 1 through 8 of the mouse Smn1 (survival motor neuron 1) gene were replaced with lacZ and an FRT site remaining from the deletion of a selection marker. [MGI Ref ID J:135422] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Smn1tm2Mrph, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Murphy A (Regeneron Pharmaceuticals, Inc.). 2008. Smn1 deletion with lacZ knock in. Personal Communication :. [MGI Ref ID J:135422]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
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General Terms and Conditions
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Contact information
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| fax: | 207-288-6655 |
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