Strain Name: |
B6.129(FVB)-Col1a2tm1Mcbr/J |
|---|---|
Stock Number: |
007248 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Col1a2; Col1a2tm1Mcbr; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x +/+ sibling (Female x Male) Species laboratory mouse Donating Investigator Daniel McBride, University of Maryland, Schl of Medicine Generation N5+F1 (03-DEC-07) Strain Description
Mice that are heterozygous for the Col1a2tm1Mcbr targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. They exhibit reduced body mass, and reduced bone mass density by 2 months of age. Homozygotes do not survive to weaning age. These mutant mice express mutant type I collagen similar to that observed in humans with osteogenesis imperfecta. Incorporation of the point mutation was verified by sequence analysis. Mutant mRNA is detected by RT-PCR analysis of total RNA. This mutant mouse strain may be useful in studies of osteogenesis imperfecta.Strain Development
A targeting vector containing a loxP flanked neomycin resistance cassette was used to introduce a point mutation, G610C, into exon 35. The construct was electroporated into 129/SvEv derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient C57BL/6J blastocysts. The resulting chimeric animals were bred to C57BL/6J. Male animals were then crossed to B6.FVB-Tg(EIIa-cre)C5379Lmgd/J female mice (Stock No. 003724) to remove the selection cassette. The mice were backcrossed to C57BL/6J for 4 generations before arriving at The Jackson Laboratory. Upon arrival the mice were backcrossed to C57BL/6J for 1 generation.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Col1a2tm1Mcbr | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Daniel J McBride | ||
| Common Name(s) | G610C Neo-; | ||
| Mutation Made By | Daniel McBride, University of Maryland, Schl of Medicine | ||
| Strain of Origin | 129/SvEv | ||
| ES Cell Line Strain | 129 | ||
| Gene Symbol and Name | Col1a2, collagen, type I, alpha 2 | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AA960264; AI325291; Col1a-2; Cola-2; Cola2; OI4; expressed sequence AA960264; expressed sequence AI325291; oim; osteogenesis imperfecta; procollagen type I, alpha 2; | ||
| Molecular Note | A targeting vector containing a loxP flanked neomycin resistance cassette was used to introduce a point mutation, G610C, into exon 35. [MGI Ref ID J:121691] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as heterozygotes. Homozygotes do not survive to weaning. The donating investigator uses a breeding scheme of heterozygote X wildtype. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Col1a2
001815 B6C3Fe a/a-Col1a2oim/J View Strains carrying other alleles of Col1a2 (1 strain)
Additional Web Information
Congenic Nomenclature
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Cell Biology Research
Defects in Extracellular Matrix Molecules
Developmental Biology Research
Defects in Extracellular Matrix Molecules
Skeletal Defects
References
Selected Reference(s)
McBride DJ. 2007. A knock-in mutation of Col1a2 MGI Direct Data Submission :. [MGI Ref ID J:121691]
Price and Supply Information
| Strain Name: | B6.129(FVB)-Col1a2tm1Mcbr/J |
| Stock Number: | 007248 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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