Strain Name: |
B6.129-Mapttm1Hnd/J |
|---|---|
Stock Number: |
007251 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Mapt; Mapttm1Hnd; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Michael Vitek, Duke University Medical Center Generation N9+F2 (08-JAN-08) Strain Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by RT-PCR analysis of total brain RNA, Western blot analysis of total brain homogenates or immunostraining of coronal brain sections. Hippocampal neurons from homozygous embryos, in primary culture, have delayed axonal extension and shorter total dendritic length when compared to wildtype controls. Mitochondria in the primary culture cells cluster at the distal end of axons. The frequency and velocity of mitochondrial anterograde movements is increased.This mutant mouse strain may be useful in studies of neuronal development, axonogenesis, and organelle movement.
Strain Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 1. The construct was electroporated into 129X1/SvJ derived embryonic stem (ES) cells from Genome Systems. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for at least 8 generations.
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Mapttm1Hnd | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Hana N Dawson | ||
| Common Name(s) | TAU-; | ||
| Mutation Made By | Michael Vitek, Duke University Medical Center | ||
| Gene Symbol and Name | Mapt, microtubule-associated protein tau | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | AI413597; AW045860; DDPAC; FLJ31424; FTDP-17; MAPTL; MGC138549; MGC156663; MSTD; MTBT1; MTBT2; Mtapt; PPND; RNPTAU; TAU; Tau; expressed sequence AI413597; expressed sequence AW045860; pTau; | ||
| Molecular Note | Exon 1, encoding the translational start site, was replaced by a neomycin selection cassette via homologous recombination. RT-PCR analysis of total brain RNA obtained from homozygous mutant mice showed a lack of transcript produced by the targeted locus.The absence of encoded protein was verified by Western blot analysis of brain homogenates as well as by immunocytochemical analysis of coronal sections. [MGI Ref ID J:78649] | ||
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Mapttm1Hnd
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as homozygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Strains carrying other alleles of Mapt
005491 B6.Cg-Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J 004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J 004779 STOCK Mapttm1(EGFP)Klt/J View Strains carrying other alleles of Mapt (3 strains)
Congenic Nomenclature
Room Number AX11
Mapttm1Hnd relatedNeurobiology Research
Alzheimer's Disease
Research Tools
Neurobiology Research
Neurobiology Research
Alzheimer's Disease (Tau (Mapt) mutants)
Selected Reference(s)
Additional ReferencesDawson HN; Ferreira A; Eyster MV; Ghoshal N; Binder LI; Vitek MP. 2001. Inhibition of neuronal maturation in primary hippocampal neurons from tau deficient mice. J Cell Sci 114(Pt 6):1179-87. [PubMed: 11228161] [MGI Ref ID J:78649]
Jimenez-Mateos EM; Gonzalez-Billault C; Dawson HN; Vitek MP; Avila J. 2006. Role of MAP1B in axonal retrograde transport of mitochondria. Biochem J 397(1):53-9. [PubMed: 16536727] [MGI Ref ID J:116415]
| Strain Name: | B6.129-Mapttm1Hnd/J |
| Stock Number: | 007251 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form