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| Hippocampal neurons (primary culture) from embryos that are homozygous for the microtubule-associated protein tau (Mapt) targeted mutation, TAU-/-, have delayed axonal extension and shorter total dendritic length and abnormal mitochondrial placement and movement. This mutant mouse strain may be useful in studies of neuronal development, axonogenesis, and organelle movement. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 13-AUG-08 Species laboratory mouse Generation N9+N1F15 (16-NOV-11)
Generation DefinitionsDonating Investigator Michael Vitek, Duke University Medical Center Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by RT-PCR analysis of total brain RNA, Western blot analysis of total brain homogenates or immunostraining of coronal brain sections. Hippocampal neurons from homozygous embryos, in primary culture, have delayed axonal extension and shorter total dendritic length when compared to wildtype controls. Mitochondria in the primary culture cells cluster at the distal end of axons. The frequency and velocity of mitochondrial anterograde movements is increased.This mutant mouse strain may be useful in studies of neuronal development, axonogenesis, and organelle movement.
Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 1. The construct was electroporated into 129X1/SvJ derived embryonic stem (ES) cells from Genome Systems. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for at least 8 generations.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Alzheimer's Disease Models
View Alzheimer's Disease Models (108 strains)
005491 B6.Cg-Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J 018282 B6.Cg-Mapttm1(Mecp2)Jae/LimmJ 012639 B6;129S4-Mapttm3(HDAC2)Jae/J 004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J 004779 STOCK Mapttm1(EGFP)Klt/J
Additional Web Information
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Frontotemporal Dementia; FTD (MAPT)
Parkinson Disease, Late-Onset; PD (MAPT)
Parkinson-Dementia Syndrome (MAPT)
Pick Disease of Brain (MAPT)
Supranuclear Palsy, Progressive, 1; PSNP1 (MAPT)
assigned by genotype
Mapttm1Hnd/Mapt+
involves: 129X1/SvJ * C57BL/6
- behavior/neurological phenotype
- decreased susceptibility to pharmacologically induced seizures
- nervous system phenotype
- decreased susceptibility to pharmacologically induced seizures
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Mapttm1Hnd/Mapttm1Hnd
involves: 129X1/SvJ * C57BL/6
- nervous system phenotype
- abnormal axon outgrowth
- neuronal cultures from E16 mice show less axonal extension than wild-type neurons; mutant neurons lag behind wild-type in development over initial 2 days in culture, then catch up, but total sum of minor processes/axonal lengths is less than wild-type (MGI Ref ID J:78649)
- abnormal dendrite morphology
- between 4 and 7 days in culture, total dendritic length of mutant neurons is less than in wild-type; after 7 days in culture, neurons still lag behind wild-type showing more developmental delay (MGI Ref ID J:78649)
- decreased susceptibility to pharmacologically induced seizures
- behavior/neurological phenotype
- decreased susceptibility to pharmacologically induced seizures
- cellular phenotype
- abnormal axon outgrowth
- neuronal cultures from E16 mice show less axonal extension than wild-type neurons; mutant neurons lag behind wild-type in development over initial 2 days in culture, then catch up, but total sum of minor processes/axonal lengths is less than wild-type (MGI Ref ID J:78649)
This mouse can be used to support research in many areas including:
Mapttm1Hnd relatedNeurobiology Research
Alzheimer's Disease
Parkinson's Disease
Research Tools
Neurobiology Research
Neurobiology Research
Alzheimer's Disease
Tau (Mapt) mutants
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Mapttm1Hnd | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Hana N Dawson | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | TAU-; | ||
| Mutation Made By | Michael Vitek, Duke University Medical Center | ||
| Strain of Origin | 129X1/SvJ | ||
| Gene Symbol and Name | Mapt, microtubule-associated protein tau | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | AI413597; AW045860; DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; Mtapt; PPND; RNPTAU; TAU; Tau; expressed sequence AI413597; expressed sequence AW045860; pTau; | ||
| Molecular Note | Exon 1, encoding the translational start site, was replaced by a neomycin selection cassette via homologous recombination. RT-PCR analysis of total brain RNA obtained from homozygous mutant mice showed a lack of transcript produced by the targeted locus.The absence of encoded protein was verified by Western blot analysis of brain homogenates as well as by immunocytochemical analysis of coronal sections. [MGI Ref ID J:78649] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Mapttm1Hnd, Melt Curve Analysis
Mapttm1Hnd, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Dawson HN; Ferreira A; Eyster MV; Ghoshal N; Binder LI; Vitek MP. 2001. Inhibition of neuronal maturation in primary hippocampal neurons from tau deficient mice. J Cell Sci 114(Pt 6):1179-87. [PubMed: 11228161] [MGI Ref ID J:78649]
Jimenez-Mateos EM; Gonzalez-Billault C; Dawson HN; Vitek MP; Avila J. 2006. Role of MAP1B in axonal retrograde transport of mitochondria. Biochem J 397(1):53-9. [PubMed: 16536727] [MGI Ref ID J:116415]
Additional References
Mapttm1Hnd relatedAndrews-Zwilling Y; Bien-Ly N; Xu Q; Li G; Bernardo A; Yoon SY; Zwilling D; Yan TX; Chen L; Huang Y. 2010. Apolipoprotein E4 causes age- and Tau-dependent impairment of GABAergic interneurons, leading to learning and memory deficits in mice. J Neurosci 30(41):13707-17. [PubMed: 20943911] [MGI Ref ID J:165492]
Barreda EG; Avila J. 2011. Tau regulates the subcellular localization of calmodulin. Biochem Biophys Res Commun 408(3):500-4. [PubMed: 21531208] [MGI Ref ID J:172402]
Dawson HN; Cantillana V; Jansen M; Wang H; Vitek MP; Wilcock DM; Lynch JR; Laskowitz DT. 2010. Loss of tau elicits axonal degeneration in a mouse model of Alzheimer's disease. Neuroscience 169(1):516-31. [PubMed: 20434528] [MGI Ref ID J:165237]
Fuster-Matanzo A; de Barreda EG; Dawson HN; Vitek MP; Avila J; Hernandez F. 2009. Function of tau protein in adult newborn neurons. FEBS Lett 583(18):3063-8. [PubMed: 19695252] [MGI Ref ID J:153129]
Gomez de Barreda E; Perez M; Gomez Ramos P; de Cristobal J; Martin-Maestro P; Moran A; Dawson HN; Vitek MP; Lucas JJ; Hernandez F; Avila J. 2010. Tau-knockout mice show reduced GSK3-induced hippocampal degeneration and learning deficits. Neurobiol Dis 37(3):622-9. [PubMed: 20004245] [MGI Ref ID J:158536]
Higuchi M; Ishihara T; Zhang B; Hong M; Andreadis A; Trojanowski J; Lee VM. 2002. Transgenic mouse model of tauopathies with glial pathology and nervous system degeneration. Neuron 35(3):433-46. [PubMed: 12165467] [MGI Ref ID J:78400]
Holth JK; Bomben VC; Reed JG; Inoue T; Younkin L; Younkin SG; Pautler RG; Botas J; Noebels JL. 2013. Tau loss attenuates neuronal network hyperexcitability in mouse and Drosophila genetic models of epilepsy. J Neurosci 33(4):1651-9. [PubMed: 23345237] [MGI Ref ID J:193896]
Jin YN; Chen PC; Watson JA; Walters BJ; Phillips SE; Green K; Schmidt R; Wilson JA; Johnson GV; Roberson ED; Dobrunz LE; Wilson SM. 2012. Usp14 deficiency increases tau phosphorylation without altering tau degradation or causing tau-dependent deficits. PLoS One 7(10):e47884. [PubMed: 23144711] [MGI Ref ID J:192359]
Lei P; Ayton S; Finkelstein DI; Spoerri L; Ciccotosto GD; Wright DK; Wong BX; Adlard PA; Cherny RA; Lam LQ; Roberts BR; Volitakis I; Egan GF; McLean CA; Cappai R; Duce JA; Bush AI. 2012. Tau deficiency induces parkinsonism with dementia by impairing APP-mediated iron export. Nat Med 18(2):291-5. [PubMed: 22286308] [MGI Ref ID J:180272]
Meilandt WJ; Yu GQ; Chin J; Roberson ED; Palop JJ; Wu T; Scearce-Levie K; Mucke L. 2008. Enkephalin elevations contribute to neuronal and behavioral impairments in a transgenic mouse model of Alzheimer's disease. J Neurosci 28(19):5007-17. [PubMed: 18463254] [MGI Ref ID J:135174]
Morris M; Koyama A; Masliah E; Mucke L. 2011. Tau reduction does not prevent motor deficits in two mouse models of Parkinson's disease. PLoS One 6(12):e29257. [PubMed: 22206005] [MGI Ref ID J:182343]
Nakamura K; Greenwood A; Binder L; Bigio EH; Denial S; Nicholson L; Zhou XZ; Lu KP. 2012. Proline isomer-specific antibodies reveal the early pathogenic tau conformation in Alzheimer's disease. Cell 149(1):232-44. [PubMed: 22464332] [MGI Ref ID J:186082]
Palop JJ; Chin J; Roberson ED; Wang J; Thwin MT; Bien-Ly N; Yoo J; Ho KO; Yu GQ; Kreitzer A; Finkbeiner S; Noebels JL; Mucke L. 2007. Aberrant excitatory neuronal activity and compensatory remodeling of inhibitory hippocampal circuits in mouse models of Alzheimer's disease. Neuron 55(5):697-711. [PubMed: 17785178] [MGI Ref ID J:126808]
Rapoport M; Dawson HN; Binder LI; Vitek MP; Ferreira A. 2002. Tau is essential to beta -amyloid-induced neurotoxicity. Proc Natl Acad Sci U S A 99(9):6364-9. [PubMed: 11959919] [MGI Ref ID J:125456]
Reyes JF; Fu Y; Vana L; Kanaan NM; Binder LI. 2011. Tyrosine Nitration within the Proline-Rich Region of Tau in Alzheimer's Disease. Am J Pathol 178(5):2275-85. [PubMed: 21514440] [MGI Ref ID J:171582]
Roberson ED; Halabisky B; Yoo JW; Yao J; Chin J; Yan F; Wu T; Hamto P; Devidze N; Yu GQ; Palop JJ; Noebels JL; Mucke L. 2011. Amyloid-beta/Fyn-induced synaptic, network, and cognitive impairments depend on tau levels in multiple mouse models of Alzheimer's disease. J Neurosci 31(2):700-11. [PubMed: 21228179] [MGI Ref ID J:168226]
Roberson ED; Scearce-Levie K; Palop JJ; Yan F; Cheng IH; Wu T; Gerstein H; Yu GQ; Mucke L. 2007. Reducing endogenous tau ameliorates amyloid beta-induced deficits in an Alzheimer's disease mouse model. Science 316(5825):750-4. [PubMed: 17478722] [MGI Ref ID J:121330]
Vossel KA; Zhang K; Brodbeck J; Daub AC; Sharma P; Finkbeiner S; Cui B; Mucke L. 2010. Tau reduction prevents Abeta-induced defects in axonal transport. Science 330(6001):198. [PubMed: 20829454] [MGI Ref ID J:164887]
Yamada K; Cirrito JR; Stewart FR; Jiang H; Finn MB; Holmes BB; Binder LI; Mandelkow EM; Diamond MI; Lee VM; Holtzman DM. 2011. In vivo microdialysis reveals age-dependent decrease of brain interstitial fluid tau levels in P301S human tau transgenic mice. J Neurosci 31(37):13110-7. [PubMed: 21917794] [MGI Ref ID J:191549]
de Barreda EG; Dawson HN; Vitek MP; Avila J. 2010. Tau deficiency leads to the upregulation of BAF-57, a protein involved in neuron-specific gene repression. FEBS Lett 584(11):2265-70. [PubMed: 20338169] [MGI Ref ID J:160378]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as homozygotes. Mating System Homozygote x Homozygote (Female x Male) 13-AUG-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $177.00 Female or Male Homozygous for Mapttm1Hnd
Price per Pair (US dollars $) Pair Genotype $354.00 Homozygous for Mapttm1Hnd x Homozygous for Mapttm1Hnd Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $230.10 Female or Male Homozygous for Mapttm1Hnd
Price per Pair (US dollars $) Pair Genotype $460.20 Homozygous for Mapttm1Hnd x Homozygous for Mapttm1Hnd Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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