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Genes & Alleles

Mapt;   Mapttm1Hnd;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Donating Investigator Michael Vitek,   Duke University Medical Center Generation N9+F2 (08-JAN-08)

Strain Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by RT-PCR analysis of total brain RNA, Western blot analysis of total brain homogenates or immunostraining of coronal brain sections. Hippocampal neurons from homozygous embryos, in primary culture, have delayed axonal extension and shorter total dendritic length when compared to wildtype controls. Mitochondria in the primary culture cells cluster at the distal end of axons. The frequency and velocity of mitochondrial anterograde movements is increased.

This mutant mouse strain may be useful in studies of neuronal development, axonogenesis, and organelle movement.

Strain Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 1. The construct was electroporated into 129X1/SvJ derived embryonic stem (ES) cells from Genome Systems. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for at least 8 generations.

Mammalian Phenotype Terms assigned by genotype Mapttm1Hnd/Mapt+         involves: C57BL/6 behavior/neurological phenotype resistance to pharmacologically induced seizures (MGI Ref ID J:121330) severity is reduced and seizure onset is delayed after pentylenetetrazole treatment compared to wild-type mice mice are resistant to kainate-induced seizures over a larger range of doses than wild-type mice homeostasis/metabolism phenotype resistance to pharmacologically induced seizures (MGI Ref ID J:121330) severity is reduced and seizure onset is delayed after pentylenetetrazole treatment compared to wild-type mice mice are resistant to kainate-induced seizures over a larger range of doses than wild-type mice nervous system phenotype resistance to pharmacologically induced seizures (MGI Ref ID J:121330) severity is reduced and seizure onset is delayed after pentylenetetrazole treatment compared to wild-type mice mice are resistant to kainate-induced seizures over a larger range of doses than wild-type mice Mapttm1Hnd/Mapttm1Hnd         involves: C57BL/6 behavior/neurological phenotype resistance to pharmacologically induced seizures (MGI Ref ID J:121330) severity is reduced and seizure onset is delayed after pentylenetetrazole treatment compared to wild-type mice mice are resistant to kainate-induced seizures over a larger range of doses than wild-type mice homeostasis/metabolism phenotype resistance to pharmacologically induced seizures (MGI Ref ID J:121330) severity is reduced and seizure onset is delayed after pentylenetetrazole treatment compared to wild-type mice mice are resistant to kainate-induced seizures over a larger range of doses than wild-type mice nervous system phenotype resistance to pharmacologically induced seizures (MGI Ref ID J:121330) severity is reduced and seizure onset is delayed after pentylenetetrazole treatment compared to wild-type mice mice are resistant to kainate-induced seizures over a larger range of doses than wild-type mice The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Mapttm1Hnd/Mapttm1Hnd         involves: 129X1/SvJ * C57BL/6 nervous system phenotype abnormal axon outgrowth (MGI Ref ID J:78649) neuronal cultures from E16 mice show less axonal extension than wild-type neurons; mutant neurons lag behind wild type in development over initial 2 days in culture, then catch up, but total sum of minor processes/axonal lengths is less than wild-type abnormal dendrite morphology (MGI Ref ID J:78649) between 4 and 7 days in culture, total dendritic length of mutant neurons is less than in wild-type; after 7 days in culture, neurons still lag behind wild-type showing more developmental delay

Gene & Allele Details

Allele Symbol		Mapttm1Hnd
Allele Name		targeted mutation 1, Hana N Dawson
Common Name(s)		TAU-;
Mutation Made By		Michael Vitek,   Duke University Medical Center
Gene Symbol and Name		Mapt, microtubule-associated protein tau
Chromosome		11
Gene Common Name(s)		AI413597; AW045860; DDPAC; FLJ31424; FTDP-17; MAPTL; MGC138549; MGC156663; MSTD; MTBT1; MTBT2; Mtapt; PPND; RNPTAU; TAU; Tau; expressed sequence AI413597; expressed sequence AW045860; pTau;
Molecular Note		Exon 1, encoding the translational start site, was replaced by a neomycin selection cassette via homologous recombination. RT-PCR analysis of total brain RNA obtained from homozygous mutant mice showed a lack of transcript produced by the targeted locus.The absence of encoded protein was verified by Western blot analysis of brain homogenates as well as by immunocytochemical analysis of coronal sections. [MGI Ref ID J:78649]

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Genotyping Protocols

Mapt^tm1Hnd

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred as homozygotes.
Diet Information	LabDiet® 5K52/5K67

Related Strains

Strains carrying other alleles of Mapt

005491	B6.Cg-Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
004808	STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
004779	STOCK Mapttm1(EGFP)Klt/J

View Strains carrying other alleles of Mapt     (3 strains)

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Alzheimer's Disease

Research Tools
Neurobiology Research

Mapt^tm1Hnd related

Neurobiology Research
Alzheimer's Disease (Tau (Mapt) mutants)

References

Selected Reference(s)

Dawson HN; Ferreira A; Eyster MV; Ghoshal N; Binder LI; Vitek MP. 2001. Inhibition of neuronal maturation in primary hippocampal neurons from tau deficient mice. J Cell Sci 114(Pt 6):1179-87. [PubMed: 11228161]  [MGI Ref ID J:78649]

Jimenez-Mateos EM; Gonzalez-Billault C; Dawson HN; Vitek MP; Avila J. 2006. Role of MAP1B in axonal retrograde transport of mitochondria. Biochem J 397(1):53-9. [PubMed: 16536727]  [MGI Ref ID J:116415]

Additional References

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Price and Supply Information

Strain Name:	B6.129-Mapttm1Hnd/J
Stock Number:	007251

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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