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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Consomic; Additional information on Consomic Mice. Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse and M. m. domesticus poschiavinus Donating Investigator Eva Eicher, The Jackson Laboratory Development
Tg(Sry-EGFP)92Ei was generated via random insertion after micro-injection into the pronucleus of C57BL/6J fertilized eggs and resulted in stock #003927, C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ. Tg(Sry)4Ei was also generated via random insertion after micro-injection into the male pronucleus of fertilized eggs from C57BL/6J females bred with C57BL/6J-Chr YPOS hermaphrodites. These strains were bred together to produce this strain and sperm was cryopreserved from a male homozygous for Tg(Sry-EGFP)92Ei and carrying both Tg(Sry)4Ei and Chr YPOS.
Related Strains
Strains carrying Tg(Sry)4Ei allele
002183 C57BL/6JEi-Tg(Sry)4Ei Chr YPOS/EiJ View Strains carrying Tg(Sry)4Ei (1 strain)
007484 B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ 007265 C57BL/6-Tg(Sry-EGFP)92Ei Chr YAKR/J/EiJ 003927 C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ 007483 FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
Phenotype
Phenotype Information
- Potential model based on transgenic expression of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. 46,xx Sex Reversal 1; SRXX1 (SRY)
46,xx Sex Reversal 2; SRXX2 (SRY)
46,xy Sex Reversal 1; SRXY1 (SRY)
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Sry)4Ei/0
C57BL/6JEi-Tg(Sry)4Ei Chr YPOS/EiJ
- reproductive system phenotype
- primary sex reversal
Tg(Sry-EGFP)92Ei/0
C57BL/6J-Tg(Sry-EGFP)92Ei
- normal phenotype
- no abnormal phenotype detected
- mice have no overt abnormal phenotype; marker expression is spatially similar to endogenous Sry expression (MGI Ref ID J:73280)
This mouse can be used to support research in many areas including:Tg(Sry-EGFP)92Ei related
Developmental Biology Research
Research Tools
Fluorescent Proteins
Reproductive Biology Research
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Sry)4Ei | ||
|---|---|---|---|
| Allele Name | transgene insertion 4, Eva M Eicher | ||
| Allele Type | Transgenic (random, expressed) | ||
| Strain of Origin | C57BL/6J-Chr YPOS | ||
| Site of Expression | Fluorescence is detected in pre-sertoli and pre-granulosa cells of the genital ridge, expression is stronger in homozygotes. Expression is also detected in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males but not females. | ||
| Expressed Gene | Sry, sex determining region of Chr Y, mouse, laboratory | ||
| Promoter | Sry, sex determining region of Chr Y, mouse, laboratory | ||
| General Note | The single founder male carried two independent multiple transgene insertions designated Tg(Sry)4Ei (with a high copy number) and Tg(Sry)5Ei (with a low copy number). Only transgenics carrying Tg(Sry)4Ei alone were used in subsequent experiments. | ||
| Molecular Note | This transgene consists of an inbred 129 strain derived 14.6 Kb genomic fragment containing the entire Sry open reading frame and no other genes. [MGI Ref ID J:31072] | ||
| Allele Symbol | Tg(Sry-EGFP)92Ei | ||
| Allele Name | transgene insertion 92, Eva M Eicher | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Tg92Ei; | ||
| Strain of Origin | C57BL/6J | ||
| Site of Expression | Fluorescence is detected in pre-sertoli and pre-granulosa cells of the genital ridge, expression is stronger in homozygotes. Expression is also detected in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males but not females. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Sry, sex determining region of Chr Y, mouse, laboratory | ||
| Molecular Note | The transgenic vector was composed of the Sry promoter region from bp 542-8304 inserted upstream of the EGFP-1 coding region. The construct contains two SV40 early mRNA polyadenylation signal in place of the endogenous Sry 3' UTR. Expression of EGFP indicates that the spatial expression of EGFP mRNA and protein is similar to endogenous Sry. [MGI Ref ID J:73280] | ||
| Gene Symbol and Name | Tg(Sry-EGFP)92Ei, transgene insertion 92, Eva M Eicher | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg92Ei; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Albrecht KH; Eicher EM. 2001. Evidence that sry is expressed in pre-sertoli cells and sertoli and granulosa cells have a common precursor. Dev Biol 240(1):92-107. [PubMed: 11784049] [MGI Ref ID J:73280]
Eicher EM; Shown EP; Washburn LL. 1995. Sex reversal in C57BL/6J-YPOS mice corrected by a Sry transgene. Philos Trans R Soc Lond B Biol Sci 350(1333):263-8. [PubMed: 8570690] [MGI Ref ID J:31072]
Additional References
Tg(Sry-EGFP)92Ei relatedBouma GJ; Hudson QJ; Washburn LL; Eicher EM. 2010. New candidate genes identified for controlling mouse gonadal sex determination and the early stages of granulosa and Sertoli cell differentiation. Biol Reprod 82(2):380-9. [PubMed: 19864314] [MGI Ref ID J:156788]
Dewing P; Chiang CW; Sinchak K; Sim H; Fernagut PO; Kelly S; Chesselet MF; Micevych PE; Albrecht KH; Harley VR; Vilain E. 2006. Direct regulation of adult brain function by the male-specific factor SRY. Curr Biol 16(4):415-20. [PubMed: 16488877] [MGI Ref ID J:123875]
Jameson SA; Natarajan A; Cool J; DeFalco T; Maatouk DM; Mork L; Munger SC; Capel B. 2012. Temporal transcriptional profiling of somatic and germ cells reveals biased lineage priming of sexual fate in the fetal mouse gonad. PLoS Genet 8(3):e1002575. [PubMed: 22438826] [MGI Ref ID J:183476]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2450.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3185.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Payment Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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JAX® Mice, Products & Services Conditions of Use
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