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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Consomic; Additional information on Consomic Mice. Type Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse and M. m. domesticus poschiavinus Donating Investigator Eva Eicher, The Jackson Laboratory Development
Tg(Sry-EGFP)92Ei was generated via random insertion after micro-injection into the pronucleus of C57BL/6J fertilized eggs and resulted in stock #003927, C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ. Tg(Sry)4Ei was also generated via random insertion after micro-injection into the male pronucleus of fertilized eggs from C57BL/6J females bred with C57BL/6J-Chr YPOS hermaphrodites. These strains were bred together to produce this strain and sperm was cryopreserved from a male homozygous for Tg(Sry-EGFP)92Ei and carrying both Tg(Sry)4Ei and Chr YPOS.
Related Strains
Strains carrying Tg(Sry)4Ei allele
002183 C57BL/6JEi-Tg(Sry)4Ei Chr YPOS/EiJ View Strains carrying Tg(Sry)4Ei (1 strain)
007484 B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ 007265 C57BL/6-Tg(Sry-EGFP)92Ei Chr YAKR/J/EiJ 003927 C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ 007483 FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
007250 C57BL/6JEi-Chr YAKR/J/Ei 001543 C57BL/6JEi-Chr YCB/EiJ
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Sry)4Ei/0
C57BL/6JEi-Tg(Sry)4Ei Chr YPOS/EiJ
- reproductive system phenotype
- primary sex reversal (MGI Ref ID J:31072)
- chromosomal females carrying this transgene develop as males
- chromosomal males that would have developed as sex-reversed females, due to the m. poschiavinus Y chromosome, develop as males when carrying this transgene
Tg(Sry-EGFP)92Ei/0
C57BL/6J-Tg(Sry-EGFP)92Ei
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:73280)
- mice have no overt abnormal phenotype; marker expression is spatially similar to endogenous Sry expression
This mouse can be used to support research in many areas including:Tg(Sry-EGFP)92Ei related
Developmental Biology Research
Neurobiology Research
Research Tools
Fluorescent Proteins
Reproductive Biology Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Sry)4Ei | ||
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| Allele Name | transgene insertion 4, Eva M Eicher | ||
| Allele Type | Transgenic (random, expressed) | ||
| Strain of Origin | C57BL/6J-Chr YPOS | ||
| Site of Expression | Fluorescence is detected in pre-sertoli and pre-granulosa cells of the genital ridge, expression is stronger in homozygotes. Expression is also detected in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males but not females. | ||
| General Note | The single founder male carried two independent multiple transgene insertions designated Tg(Sry)4Ei (with a high copy number) and Tg(Sry)5Ei (with a low copy number). Only transgenics carrying Tg(Sry)4Ei alone were used in subsequent experiments. | ||
| Molecular Note | This transgene consists of an inbred 129 strain derived 14.6 Kb genomic fragment containing the entire Sry open reading frame and no other genes. [MGI Ref ID J:31072] | ||
| Allele Symbol | Tg(Sry-EGFP)92Ei | ||
| Allele Name | transgene insertion 92, Eva M Eicher | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Tg92Ei; | ||
| Strain of Origin | C57BL/6J | ||
| Site of Expression | Fluorescence is detected in pre-sertoli and pre-granulosa cells of the genital ridge, expression is stronger in homozygotes. Expression is also detected in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males but not females. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Sry, sex determining region of Chr Y, mouse, laboratory | ||
| Molecular Note | The transgenic vector was composed of the Sry promoter region from bp 542-8304 inserted upstream of the EGFP-1 coding region. The construct contains two SV40 early mRNA polyadenylation signal in place of the endogenous Sry 3' UTR. Expression of EGFP indicates that the spatial expression of EGFP mRNA and protein is similar to endogenous Sry . [MGI Ref ID J:73280] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), MCA, vers. 2
Fluorescent Proteins (Generic GFP), STD PCR, vers. 1
Tg(Sry) Chr Y, , vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Albrecht KH; Eicher EM. 2001. Evidence that sry is expressed in pre-sertoli cells and sertoli and granulosa cells have a common precursor. Dev Biol 240(1):92-107. [PubMed: 11784049] [MGI Ref ID J:73280]
Eicher EM; Shown EP; Washburn LL. 1995. Sex reversal in C57BL/6J-YPOS mice corrected by a Sry transgene. Philos Trans R Soc Lond B Biol Sci 350(1333):263-8. [PubMed: 8570690] [MGI Ref ID J:31072]
Additional References
Tg(Sry-EGFP)92Ei relatedDewing P; Chiang CW; Sinchak K; Sim H; Fernagut PO; Kelly S; Chesselet MF; Micevych PE; Albrecht KH; Harley VR; Vilain E. 2006. Direct regulation of adult brain function by the male-specific factor SRY. Curr Biol 16(4):415-20. [PubMed: 16488877] [MGI Ref ID J:123875]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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General Terms and Conditions
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