Strain Name: |
C57BL/6-Tg(Sry-EGFP)92Ei Chr YAKR/J/EiJ |
|---|---|
Stock Number: |
007265 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | GFP; Sry; Tg(Sry-EGFP)92Ei; |
Product Information
Strain Details
Type Consomic Additional information on Consomic Mice. Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Eva Eicher, The Jackson Laboratory Generation N15F2pN1 Strain Development
Tg(Sry-EGFP)92Ei was generated via random insertion after micro-injection into the pronucleus of C57BL/6J fertilized eggs. The resulting transgenic strain, C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ (Stock No. 003927), was bred to homozygosity and a female was bred to a male C57BL/6J-Chr YAKR/J/EiJ (Stock No. 007250) when that consomic was at generation N56. The progeny were backcrossed to homozygous C57BL/6J-Tg(Sry-EGFP)92Ei females until generation N15. In 2007 sperm were cryopreserved from a homozygous male at generation N15F2.
Gene & Allele Details
| Allele Symbol | Tg(Sry-EGFP)92Ei | ||
|---|---|---|---|
| Allele Name | transgene insertion 92, Eva M Eicher | ||
| Common Name(s) | Tg92Ei; | ||
| Strain of Origin | C57BL/6J | ||
| Site of Expression | Fluorescence is detected in pre-sertoli and pre-granulosa cells of the genital ridge, expression is stronger in homozygotes. Expression is also detected in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males but not females. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Sry, sex determining region of Chr Y, mouse, laboratory | ||
| Molecular Note | The transgenic vector was composed of the Sry promoter region from bp 542-8304 inserted upstream of the EGFP-1 coding region. The construct contains two SV40 early mRNA polyadenylation signal in place of the endogenous Sry 3' UTR. Expression of EGFP indicates that the spatial expression of EGFP mRNA and protein is similar to endogenous Sry . [MGI Ref ID J:73280] | ||
Related Strains
Strains carrying Tg(Sry-EGFP)92Ei allele
View Strains carrying Tg(Sry-EGFP)92Ei (4 strains)
007250 C57BL/6JEi-Chr YAKR/J/Ei 001543 C57BL/6JEi-Chr YCB/EiJ
Research Applications
This mouse can be used to support research in many areas including:Tg(Sry-EGFP)92Ei related
Developmental Biology Research
Neurobiology Research
Research Tools
Fluorescent Proteins
Reproductive Biology Research
References
Selected Reference(s)
Albrecht KH; Eicher EM. 2001. Evidence that sry is expressed in pre-sertoli cells and sertoli and granulosa cells have a common precursor. Dev Biol 240(1):92-107. [PubMed: 11784049] [MGI Ref ID J:73280]
Dewing P; Chiang CW; Sinchak K; Sim H; Fernagut PO; Kelly S; Chesselet MF; Micevych PE; Albrecht KH; Harley VR; Vilain E. 2006. Direct regulation of adult brain function by the male-specific factor SRY. Curr Biol 16(4):415-20. [PubMed: 16488877] [MGI Ref ID J:123875]
Price and Supply Information
| Strain Name: | C57BL/6-Tg(Sry-EGFP)92Ei Chr YAKR/J/EiJ |
| Stock Number: | 007265 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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