Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6Ei.129(Cg)-Zfpm2tm2Sho/EiJ    (Changed: 18-AUG-08 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation B6?+B6N6pN1 Generation Definitions

Description

When bred to a strain with the targeted null allele (Stock No. 005646) and a strain expressing Cre recombinase in mammary gland epithelium (see Stock No. 003552 for example), this mutant mouse strain may be useful in studies of epithelial cell differentiation in the mammary gland and in breast cancer.

When bred to a strain with the targeted null allele (Stock No. 005646) and a strain expressing Cre recombinase in endothelial cells (see Stock No. 008863 for example), this mutant mouse strain may be useful in cardiac studies.

When bred to a strain expressing Cre recombinase in cardiomyocytes (Stock No. 010911) this mutant mouse strain may be useful in cardiac studies.

Related Strains

Strains carrying other alleles of Zfpm2

005646	B6.129S1(Cg)-Zfpm2tm1Sho/EiJ
006838	D2.129S1(Cg)-Zfpm2tm1Sho/EiJ
006768	D2.Cg-Tg(Myh6-Zfpm2)1Sho/EiJ

View Strains carrying other alleles of Zfpm2     (3 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Zfpm2^tm2Sho/Zfpm2^tm2Sho

        involves: 129 * C57BL/6J

• normal phenotype
• no abnormal phenotype detected
  • mice are viable, fertile and show no obvious abnormalities   (MGI Ref ID J:120985)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX^® Services jaxservices@jax.org for customized breeding options.

Zfpm2^tm1Sho/Zfpm2^tm2Sho Tg(Tek-cre)1Ywa/0

        involves: 129S1/Sv * C57BL/6 * SJL   (conditional)

• mortality/aging
• *normal* mortality/aging
  • survival rate to weaning is normal   (MGI Ref ID J:150452)
• cardiovascular system phenotype
• *normal* cardiovascular system phenotype
  • coronary plexus, endocardial cushions and compact myocardial layer develop normally   (MGI Ref ID J:150452)
  • adults have normal tricuspid and mitral valves   (MGI Ref ID J:150452)

Zfpm2^tm1Sho/Zfpm2^tm2Sho Tg(Wap-cre)11738Mam/0

        involves: 129 * C57BL/6J * C57BL/6NCr   (conditional)

• reproductive system phenotype
• *normal* reproductive system phenotype
  • mice are fertile   (MGI Ref ID J:120985)
• • abnormal mammary gland morphology
    • at day 4 of involution, alveoli are completely collapsed into clusters of epithelial cells and the area containing adipocytes is greatly expanded   (MGI Ref ID J:120985)
    • however, after 6 days of involution there is no difference when compared to controls   (MGI Ref ID J:120985)
  • • abnormal involution of the mammary gland
      • involution occurs faster at days 3 and 4 of involution than in controls   (MGI Ref ID J:120985)
• endocrine/exocrine gland phenotype
• abnormal mammary gland morphology
  • at day 4 of involution, alveoli are completely collapsed into clusters of epithelial cells and the area containing adipocytes is greatly expanded   (MGI Ref ID J:120985)
  • however, after 6 days of involution there is no difference when compared to controls   (MGI Ref ID J:120985)
• • abnormal involution of the mammary gland
    • involution occurs faster at days 3 and 4 of involution than in controls   (MGI Ref ID J:120985)
• integument phenotype
• abnormal mammary gland morphology
  • at day 4 of involution, alveoli are completely collapsed into clusters of epithelial cells and the area containing adipocytes is greatly expanded   (MGI Ref ID J:120985)
  • however, after 6 days of involution there is no difference when compared to controls   (MGI Ref ID J:120985)
• • abnormal involution of the mammary gland
    • involution occurs faster at days 3 and 4 of involution than in controls   (MGI Ref ID J:120985)

Wt1^{tm1(EGFP/cre)Wtp}/Wt1⁺ Zfpm2^tm1Sho/Zfpm2^tm2Sho

        involves: 129S1/Sv * 129S4/SvJae * 129S7/SvEvBrd   (conditional)

• mortality/aging
• complete perinatal lethality
  • pups die in perinatal period   (MGI Ref ID J:150452)
• cardiovascular system phenotype
• *normal* cardiovascular system phenotype
  • coronary vascular development is not impaired   (MGI Ref ID J:150452)
• • abnormal atrioventricular cushion morphology
    • severe atrio-ventricular endocardial cushion defect is observed by E14.5   (MGI Ref ID J:150452)
  • thin myocardium compact layer
    • compact myocardium is thin   (MGI Ref ID J:150452)

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Zfpm2tm2Sho
Allele Name		targeted mutation 2, Stuart Orkin
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		Fog2flox;
Strain of Origin		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line Name		CJ7
ES Cell Line Strain		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name		Zfpm2, zinc finger protein, multitype 2
Chromosome		15
Gene Common Name(s)		B330005D23Rik; DIH3; FOG-2; FOG2; RIKEN cDNA B330005D23 gene; ZNF89B; hFOG-2;
Molecular Note		A loxP site was inserted into the 7th intron and a second loxP site was inserted into the XbaI site of the 3' downstream sequence. [MGI Ref ID J:120985]

Genotyping

Genotyping Information

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Manuylov NL; Smagulova FO; Tevosian SG. 2007. Fog2 excision in mice leads to premature mammary gland involution and reduced Esr1 gene expression Oncogene 26(36):5204-13. [PubMed: 17310981]  [MGI Ref ID J:120985]

Additional References

Zfpm2^tm2Sho related

Zhou B; Ma Q; Kong SW; Hu Y; Campbell PH; McGowan FX; Ackerman KG; Wu B; Zhou B; Tevosian SG; Pu WT. 2009. Fog2 is critical for cardiac function and maintenance of coronary vasculature in the adult mouse heart. J Clin Invest 119(6):1462-76. [PubMed: 19411759]  [MGI Ref ID J:150452]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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