Strain Name:

B6.129X1-Wasf1tm2Jsco/J

Stock Number:

007446

Availability:

Repository-Cryopreserved

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
 
Donating Investigator John Scott,   Oregon Health Science University

Description
Homozygotes are viable, healthy and normal in size. The level of mutant protein expressed by homozygous mice is similar to that of wildtype animals as determined by immunoprecipitation of brain extracts, but the level of protein binding to Rac GTPase-activating protein (WRP; formally SRGAP3) is reduced by 50%. The mice have reduced spine density in CA1 of the hippocampus and layer of the cortex. There is also an elevated late-phase long-term potentiation (L-LTP) and mild impairment in memory retention.

Development
A targeting vector was designed to place a neomycin cassette in intron 6, and delete amino acids 322-332 and 425-431 of exon 7. The vector was introduced to 129X1/SvJ-derived JSI-1 embryonic stem (ES) cells. Chimeric mice were generated and backcrossed to C57BL/6 for at least 10 generations by the donating laboratory.

Related Strains

Strains carrying other alleles of Wasf1
007445   B6.129X1-Wasf1tm1Jsco/J
View Strains carrying other alleles of Wasf1     (1 strain)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Wasf1tm2Jsco/Wasf1tm2Jsco

        involves: 129X1/SvJ * C57BL/6
  • behavior/neurological phenotype
  • abnormal response to novel object (MGI Ref ID J:117303)
    • mutants spend less time exploring a novel object compared to wild-type littermates in novelty recognition tests, showing impairment in nonspatial memory
  • abnormal spatial learning (MGI Ref ID J:117303)
    • memory retention is impaired in Morris water maze; mutants spend less time searching in target quadrant after removal of platform in probe trials on days 3 and 4 of training; after 1 additional day of training, mutants can distinguish between target and nontarget quadrants
    • when tested 14 days after last hidden platform training day, mutants exhibit loss of spatial memory compared with wild-type and heterozygous mice; memory formation occurs, but is less stable
  • nervous system phenotype
  • *normal* nervous system phenotype (MGI Ref ID J:117303)
    • early-phase LTP and LTD is normal in knockin mice; paired-pulse facilitation is normal
    • abnormal excitatory postsynaptic potential (MGI Ref ID J:117303)
      • hippocampal slices from mutants display a reduction in maximal fEPSPs, particularly at higher stimulus intensities
    • abnormal neuron morphology (MGI Ref ID J:117303)
      • in vivo, CA1 hipoocampal and cortical neurons in 10-week old mice show a reduction in dendritic spine density (103 spines/100 um or 91 spines/100 um in wild-type hippocampal or cortical neurons (layer 1) compared to 88 spines/100 um or 68.4 spines/100 um in mutant hippocampal or cortical neurons)
    • enhanced long term potentiation (MGI Ref ID J:117303)
      • L-LTP is increased (seen in responses at 150-180 minutes)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Behavioral and Learning Defects
Neurodevelopmental Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol Wasf1tm2Jsco
Allele Name targeted mutation 2, John D Scott
Allele Type Targeted (knock-in)
Common Name(s) wave ;
Mutation Made By John Scott,   Oregon Health Science University
Strain of Origin129X1/SvJ
ES Cell Line NameOther (see notes)
ES Cell Line Strain129
Gene Symbol and Name Wasf1, WASP family 1
Chromosome 10
Gene Common Name(s) AI195380; AI838537; FLJ31482; KIAA0269; MGC109303; SCAR1; Scar; WAVE; WAVE-1; WAVE1; expressed sequence AI195380; expressed sequence AI838537;
General Note ES cell line = JSI-1.
Molecular Note A targeting vector was designed to place a neomycin cassette in intron 6, and delete amino acids 322-332 and 425-431 of exon 7. The vector was introduced to 129X1/SvJ-derived JSI-1 embryonic stem (ES) cells. Protein levels are similar to wild-type as shown by immunoprecipitation, but the level of protein binding to Rac GTPase-activating protein (WRP) is reduced by 50%. [MGI Ref ID J:117303]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Soderling SH; Guire ES; Kaech S; White J; Zhang F; Schutz K; Langeberg LK; Banker G; Raber J; Scott JD. 2007. A WAVE-1 and WRP signaling complex regulates spine density, synaptic plasticity, and memory. J Neurosci 27(2):355-65. [PubMed: 17215396]  [MGI Ref ID J:117303]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.

General Terms and Conditions


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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