Strain Name: |
B6.129-Akap6tm1Jsco/14J |
|---|---|
Stock Number: |
007447 |
Availability: | Under Development for Cryopreservation Repository |
| To register your interest in this strain go to the Strain Interest Form. |
General Terms and Conditions |
| Genes & Alleles | Akap6; Akap6tm1Jsco; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator John Scott, Oregon Health Science University Strain Description
Homozygous mice have reduced viability, especially on a C57BL/6 background. Approximately 1 out of 4 homozygotes are very small and die before reaching 2 weeks of age. The remaining homozygotes are 20-30% smaller than wildtype and heterozygous littermates; these survive and eventually become similar in size to wildtypes. All homozygotes have a characteristic craniofacial defect that includes a very pointed nose, eyes that are delayed in opening, and a shortened jaw. The alpha transcript of the gene is absent, as determined by brain mRNA and protein assays. Beta transcript RNA is present in heart and brain.Strain Development
A targeting vector was designed to replace exon 2 with an IRES, LacZ, URA and neomycin cassette and thus inhibit the production of the alpha alternative transcript. The vector was injected into 129-derived embryonic stem (ES) cells. This line (line T14) has been backcrossed to C57BL/6 for at least 10 generations.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Akap6tm1Jsco | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, John D Scott | ||
| Common Name(s) | mAKAP alpha<->; | ||
| Mutation Made By | John Scott, Oregon Health Science University | ||
| Strain of Origin | 129 | ||
| Gene Symbol and Name | Akap6, A kinase (PRKA) anchor protein 6 | ||
| Chromosome | 12 | ||
| Gene Common Name(s) | ADAP100; ADAP6; AI482140; AKAP100; KIAA0311; MGC165020; MGC:73457; PRKA; PRKA6; anchor protein 6; expressed sequence AI482140; mAKAP; | ||
| Molecular Note | A targeting vector was designed to replace exon 2 with an IRES, LacZ, URA and neomycin cassette and thus inhibit the production of the alpha alternative transcript. The vector was injected into 129-derived embryonic stem (ES) cells. [MGI Ref ID J:121227] | ||
Colony Maintenance
| Breeding & Husbandry | When maintained as a live colony, heterozygotes may be bred. Homozygotes have reduced viability. |
|---|
Related Strains
Strains carrying Akap6tm1Jsco allele
007448 B6.129-Akap6tm1Jsco/38J View Strains carrying Akap6tm1Jsco (1 strain)
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:
Developmental Biology Research
Craniofacial and Palate Defects
Postnatal Mortality (Homozygous)
References
Selected Reference(s)
Michel JJ; Townley IK; Dodge-Kafka KL; Zhang F; Kapiloff MS; Scott JD. 2005. Spatial restriction of PDK1 activation cascades by anchoring to mAKAPalpha. Mol Cell 20(5):661-72. [PubMed: 16337591] [MGI Ref ID J:121227]
Price and Supply Information
| Strain Name: | B6.129-Akap6tm1Jsco/14J |
| Stock Number: | 007447 |
This strain is currently Under Development for Cryopreservation Repository.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development
Supply Details
| Standard Supply | Under Development for Cryopreservation Repository |
|---|---|
| Supply Notes |
This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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