Strain Name: |
B6.129-Akap6tm1Jsco/38J |
|---|---|
Stock Number: |
007448 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Akap6; Akap6tm1Jsco; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator John Scott, Oregon Health Science University Strain Description
Homozygous mice have reduced viability, especially on a C57BL/6 background. Approximately 1 out of 4 homozygotes are very small and die before reaching 2 weeks of age. The remaining homozygotes are 20-30% smaller than wildtype and heterozygous littermates; these survive and eventually become similar in size to wildtypes. All homozygotes have a characteristic craniofacial defect that includes a very pointed nose, eyes that are delayed in opening, and a shortened jaw. The alpha transcript of the gene is absent, as determined by brain mRNA and protein assays. Beta transcript RNA is present in heart and brain.Strain Development
A targeting vector was designed to replace exon 2 with an IRES, LacZ, URA and neomycin cassette and thus inhibit the production of the alpha alternative transcript. The vector was injected into 129-derived embryonic stem (ES) cells. This line (line T38) has been backcrossed to C57BL/6 for at least 10 generations.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Akap6tm1Jsco | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, John D Scott | ||
| Common Name(s) | mAKAP alpha<->; | ||
| Mutation Made By | John Scott, Oregon Health Science University | ||
| Strain of Origin | 129 | ||
| Gene Symbol and Name | Akap6, A kinase (PRKA) anchor protein 6 | ||
| Chromosome | 12 | ||
| Gene Common Name(s) | ADAP100; ADAP6; AI482140; AKAP100; KIAA0311; MGC165020; MGC:73457; PRKA; PRKA6; anchor protein 6; expressed sequence AI482140; mAKAP; | ||
| Molecular Note | A targeting vector was designed to replace exon 2 with an IRES, LacZ, URA and neomycin cassette and thus inhibit the production of the alpha alternative transcript. The vector was injected into 129-derived embryonic stem (ES) cells. [MGI Ref ID J:121227] | ||
Colony Maintenance
| Breeding & Husbandry | When maintained as a live colony, heterozygotes may be bred. Homozygotes have reduced viability. |
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Related Strains
Strains carrying Akap6tm1Jsco allele
007447 B6.129-Akap6tm1Jsco/14J View Strains carrying Akap6tm1Jsco (1 strain)
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:
Developmental Biology Research
Craniofacial and Palate Defects
Postnatal Mortality
References
Selected Reference(s)
Michel JJ; Townley IK; Dodge-Kafka KL; Zhang F; Kapiloff MS; Scott JD. 2005. Spatial restriction of PDK1 activation cascades by anchoring to mAKAPalpha. Mol Cell 20(5):661-72. [PubMed: 16337591] [MGI Ref ID J:121227]
Price and Supply Information
| Strain Name: | B6.129-Akap6tm1Jsco/38J |
| Stock Number: | 007448 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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