Strain Name:

FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ

Stock Number:

007483

Availability:

Repository-Cryopreserved

Description

Strain Information

Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
GenerationN10F?pN1
 
Donating Investigator Eva Eicher,   The Jackson Laboratory

Appearance
albino female
Related Genotype: A/A Tyrc/Tyrc +/+

albino male
Related Genotype: A/A Tyrc/Tyrc +/Y

mottled gray-brown female
Related Genotype: A/A Tyrc/Tyrc Tg(Tyr)3412ARpw/+

dark gray-brown male
Related Genotype: A/A Tyrc/Tyrc Tg(Tyr)3412ARpw/Y

View images of coat colors for this strain.

Description
On an albino background Tg(Tyr)3412ARpw permits the identification of gender as early as embryonic day 10.5. This strain is segregating for Tg(Tyr)3412ARpw and homozygous for Tyrc-2J so the individuals not carrying Tg(Tyr3412)ARpw are albino. Because Tg(Tyr)3412ARpw inserted into the X Chromosome, breeding a carrier male with a noncarrier (wild-type) female results in embryos in which all XX individuals develop eye pigment, due to the Tg(Tyr)3412ARpw inherited from their father, while all XY individuals have non-pigmented eyes, having inherited a wild-type X Chromosome from their mother.

This strain is also homozygous for Tg(SryEGFP)92Ei. This reporter transgene consists of a 5' regulatory segment of the Sry gene driving EGFP. This transgene is expressed in the pre-support cell lineage (pre-sertoli and pre-granulosa cells) of the fetal genital ridge (Albrecht and Eicher, 2001) and in discrete areas the adult male but not female brain (Dewing et al., 2006). Specifically, Sry has been found expressed in tyrosine hydroxylase expressing neurons in all regions of the male substantial nigra, most strongly in the pars compacta. The expression of EGFP from this transgene is stronger in homozygotes than in heterozygotes. This strain is maintained homozygous for Tg(SryEGFP)92Ei.

Development
Tg(Tyr)3412ARpw was generated by microinjection into FVB/N fertilized eggs and integrated into the X Chromosome. Tg(Sry-EGFP)92Ei was generated by microinjection into C57BL/6J fertilized eggs. Tg(Sry-EGFP)92Ei was later backcrossed onto the FVB/NJ background, starting by crossing an FVB/NJ female with a C57BL/6J-Tg(Sry-EGFP)92Ei carrier male. The N2 backcross offspring were typed for MIT markers and Tg(Sry-EGFP)92Ei was determined to map to a position of Chromosome 5 between D5Mit255 and D5Mit197. An N2 mouse carrying Tg(Sry-EGFP)92Ei and B6 alleles of these D5Mit markers was selected to continue the backcross. At the N9 backcross generation a female carrying Tg(Sry-EGFP)92Ei and the two flanking B6-derived D5Mit loci was mated to an FVB/N-Tg(Tyr)3412ARpw male. The resulting N10 offspring were typed for Tg(Sry-EGFP)92Ei and the B6 alleles of D5Mit255 and D5Mit197 and carriers were intercrossed and their N10F2 offspring were typed to identify breeders homozygous for the B6 allele of D5Mit255 and D5Mit197. Thereafter this strain has been maintained homozygous for Tg(Sry-EGFP)92Ei and segregating for Tg(Tyr)3412ARpw. The pigment conferred to carriers of Tg(Tyr)3412ARpw on this otherwise albino background makes breeder selection easy since one need only pair up one albino mouse with a pigmented sibling.

Related Strains

Fluorescent Protein Strains
006053   129-Gt(ROSA)26Sortm1Luo/J
006067   129-Gt(ROSA)26Sortm2Luo/J
006041   129-Gt(ROSA)26Sortm3Luo/J
005483   129-Tg(CAG-EYFP)7AC5Nagy/J
003960   129S6-Tg(Prnp-GFP/cre)1Blw/J
006102   B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J
006100   B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J
005999   B6(SJL)-Tg(SBE/Tk-luc)7Twc/J
007676   B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
004178   B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J
004218   B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J
006071   B6.129-Gt(ROSA)26Sortm1Luo/J
006080   B6.129-Gt(ROSA)26Sortm2Luo/J
006075   B6.129-Gt(ROSA)26Sortm3Luo/J
006412   B6.129-Il12btm1Lky/J
008451   B6.129P(Cg)-Ptprca Cx3cr1tm1Litt/LittJ
005582   B6.129P-Cx3cr1tm1Litt/J
007572   B6.129P2(Cg)-Rorctm2Litt/J
005693   B6.129P2-Cxcr6tm1Litt/J
007669   B6.129S4-Pdgfratm11(EGFP)Sor/J
006852   B6.129S6-Per2tm1Jt/J
008577   B6.129X1-Gpr65tm1Witt/J
006148   B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J
008466   B6.129X1-Shhtm6Amc/J
003479   B6.C3-Tg(Fos-luc)1Rnd/J
006772   B6.Cg-Foxp3tm2Tch/J
005670   B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
007914   B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J
007920   B6.Cg-Gt(ROSA)26Sortm2(CAG-EYFP)Hze/J
007903   B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J
007909   B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J
005491   B6.Cg-Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
005622   B6.Cg-Shhtm1(EGFP/cre)Cjt/J
007484   B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
006055   B6.Cg-Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
006051   B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J
007575   B6.Cg-Tg(CAG-Ngb,-EGFP)1Dgrn/J
008111   B6.Cg-Tg(CAG-Ub*G76V/GFP)1Dant/J
008112   B6.Cg-Tg(CAG-Ub*G76V/GFP)2Dant/J
005884   B6.Cg-Tg(CAG-mRFP1)1F1Hadj/J
007897   B6.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
006069   B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J
005029   B6.Cg-Tg(Hlxb9-GFP)1Tmj/J
006098   B6.Cg-Tg(Il2/NFAT-luc)83Rinc/J
006864   B6.Cg-Tg(Ins1-EGFP)1Hara/J
005244   B6.Cg-Tg(Krt1-15-EGFP)2Cot/J
008323   B6.Cg-Tg(Mc4r-MAPT/GFP*)21Rck/J
007742   B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J
008321   B6.Cg-Tg(Npy-MAPT/GFP*)1Rck/J
008322   B6.Cg-Tg(Pomc-MAPT/GFP*)1Rck/J
007902   B6.Cg-Tg(RP23-268L19-EGFP)2Mik/J
007894   B6.Cg-Tg(Rgs4-EGFP)4Lvt/J
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
004659   B6.Cg-Tg(TIE2GFP)287Sato/1J
006101   B6.Cg-Tg(TRE/Prl-luc)31FlvRinc/J
007901   B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J
007911   B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J
007921   B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J
003710   B6.Cg-Tg(Thy1-CFP)23Jrs/J
007940   B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J
007612   B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J
007615   B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J
005630   B6.Cg-Tg(Thy1-EYFP)15Jrs/J
003709   B6.Cg-Tg(Thy1-YFP)16Jrs/J
005627   B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J
003782   B6.Cg-Tg(Thy1-YFPH)2Jrs/J
007606   B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J
006000   B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
004509   B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
006417   B6.FVB-Tg(Npy-hrGFP)1Lowl/J
005738   B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J
008126   B6.NOD-Tg(Cd4-EGFP)1Lt/J
004077   B6;129-Gt(ROSA)26Sortm2Sho/J
008295   B6;129-Syt9tm1Sud/J
008513   B6;129P2-Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/J
006676   B6;129P2-Olfr151tm26Mom/MomJ
006667   B6;129P2-Omptm3Mom/MomJ
004858   B6;129S1-Tshrtm1Rmar/J
007843   B6;129S4-Efnb2tm2Sor/J
008214   B6;129S4-Pou5f1tm2Jae/J
008078   B6;129S4-Tcfe2atm5Zhu/J
008605   B6;C3-Tg(CAG-DsRed,-EGFP)5Gae/J
006614   B6;CB-Tg(Thy1-CFP/COX8A)C1Lich/J
006617   B6;CB-Tg(Thy1-CFP/COX8A)S2Lich/J
004966   B6;CBA-Tg(Acrv1-EGFP)2727Redd/J
004654   B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J
007910   B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J
005621   B6;D2-Tg(S100B-EGFP)1Wjt/J
005620   B6;D2-Tg(S100B-EYFP)1Wjt/J
004690   B6;FVB-Tg(Pcp2-EGFP)2Yuza/J
006147   B6;FVB-Tg(Sfpi1,-EGFP)7Dgt/J
006043   B6;SJL-Tg(Oxt/EGFP)AI03Wsy/J
008004   B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J
007610   B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J
007880   B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J
007856   B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J
004190   C.129-Il4tm1Lky/J
005700   C.129P2-Cxcr6tm1Litt/J
006769   C.Cg-Foxp3tm2Tch/J
004512   C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
008242   C57BL/6-Gt(ROSA)26Sortm1(Ikbkb)Rsky/J
008517   C57BL/6-Gt(ROSA)26Sortm3(CAG-MIRN17-92,-EGFP)Rsky/J
006567   C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ
003291   C57BL/6-Tg(CAG-EGFP)1Osb/J
005070   C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J
004353   C57BL/6-Tg(UBC-GFP)30Scha/J
005706   C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
006618   C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J
006362   C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J
007860   C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J
007567   C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
003927   C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ
008234   CB6-Tg(CAG-EGFP/CETN2)3-4Jgg/J
007677   CB6-Tg(Gad1-EGFP)G42Zjh/J
007075   CByJ.B6-Tg(CAG-EGFP)1Osb/J
007076   CByJ.B6-Tg(UBC-GFP)30Scha/J
003718   FVB-Tg(GadGFP)45704Swn/J
005515   FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
006421   FVB-Tg(Pomc1-hrGFP)1Lowl/J
005688   FVB-Tg(Rag2-EGFP)1Mnz/J
005125   FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J
006206   FVB.129S6-Gt(ROSA)26Sortm1(HIF1A/luc)Kael/J
003516   FVB.Cg-Tg(CAG-EGFP)B5Nagy/J
008200   FVB/N-Tg(CAG-EGFP,-ALPP)2.6Ggc/J
003257   FVB/N-Tg(GFAPGFP)14Mes/J
007800   FVB/N-Tg(Ins1-luc)VUPwrs/J
006698   NOD.Cg-Il4tm1Lky/JbsJ
008173   NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ
005076   NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ
008547   NOD.FVB-Tg(ITGAM-DTR/EGFP)34Lan/JdkJ
008549   NOD.FVB-Tg(Itgax-DTR/EGFP)57Lan/JdkJ
005082   NOD/ShiLt-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ
005328   NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J
005334   NOD/ShiLt-Tg(Cd4-EGFP)1Lt/J
008694   NOD/ShiLt-Tg(Foxp3-EGFP/cre)1Jbs/J
005282   NOD/ShiLtJ-Tg(Ins1-EGFP/GH1)14Hara/HaraJ
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
005130   STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
004808   STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
004779   STOCK Mapttm1(EGFP)Klt/J
006741   STOCK Olfr160tm1Mom Tg(Olfr151,taulacZ)BMom/MomJ
006678   STOCK Olfr160tm6Mom/MomJ
006669   STOCK Olfr17tm7Mom/MomJ
006570   STOCK Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj Tg(SMN2)89Ahmb/J
007879   STOCK Stx1atm2Sud/J
005438   STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
006850   STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
006876   STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J
003920   STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
005441   STOCK Tg(CAG-DsRed*MST)1Nagy/J
003773   STOCK Tg(CAG-ECFP)CK6Nagy/J
003115   STOCK Tg(CAG-EGFP)B5Nagy/J
003116   STOCK Tg(CAG-EGFP)D4Nagy/J
005645   STOCK Tg(CAG-mRFP1)1F1Hadj/J
005105   STOCK Tg(Chx10-EGFP/cre-ALPP)2Clc/J
005854   STOCK Tg(Cp-EGFP)25Gaia/J
008241   STOCK Tg(Cspg4-DsRed.T1)1Akik/J
006334   STOCK Tg(Gad1-EGFP)94Agmo/J
006340   STOCK Tg(Gad1-EGFP)98Agmo/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
005418   STOCK Tg(HIST1H2BB/EGFP)1Pa/J
006866   STOCK Tg(Ins1-DsRed*T4)32Hara/J
006784   STOCK Tg(Ins1-ECFP)24Hara/J
003658   STOCK Tg(TIE2GFP)287Sato/J
006129   STOCK Tg(Zp3-EGFP)1Dean/J
003274   STOCK Tg(tetNZL)2Bjd/J
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005699   STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
View Fluorescent Protein Strains     (170 strains)

View Strains carrying   Tg(Sry-EGFP)92Ei     (4 strains)

Strains carrying   Tg(Tyr)3412ARpw allele
007484   B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
View Strains carrying   Tg(Tyr)3412ARpw     (1 strain)

Strains carrying other alleles of GFP
006053   129-Gt(ROSA)26Sortm1Luo/J
006067   129-Gt(ROSA)26Sortm2Luo/J
006041   129-Gt(ROSA)26Sortm3Luo/J
003960   129S6-Tg(Prnp-GFP/cre)1Blw/J
007676   B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
004178   B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J
006071   B6.129-Gt(ROSA)26Sortm1Luo/J
006080   B6.129-Gt(ROSA)26Sortm2Luo/J
006075   B6.129-Gt(ROSA)26Sortm3Luo/J
008451   B6.129P(Cg)-Ptprca Cx3cr1tm1Litt/LittJ
005582   B6.129P-Cx3cr1tm1Litt/J
005670   B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
005622   B6.Cg-Shhtm1(EGFP/cre)Cjt/J
007575   B6.Cg-Tg(CAG-Ngb,-EGFP)1Dgrn/J
008111   B6.Cg-Tg(CAG-Ub*G76V/GFP)1Dant/J
008112   B6.Cg-Tg(CAG-Ub*G76V/GFP)2Dant/J
007897   B6.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
006069   B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J
005029   B6.Cg-Tg(Hlxb9-GFP)1Tmj/J
006864   B6.Cg-Tg(Ins1-EGFP)1Hara/J
005244   B6.Cg-Tg(Krt1-15-EGFP)2Cot/J
008323   B6.Cg-Tg(Mc4r-MAPT/GFP*)21Rck/J
007742   B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J
008321   B6.Cg-Tg(Npy-MAPT/GFP*)1Rck/J
008322   B6.Cg-Tg(Pomc-MAPT/GFP*)1Rck/J
007902   B6.Cg-Tg(RP23-268L19-EGFP)2Mik/J
007894   B6.Cg-Tg(Rgs4-EGFP)4Lvt/J
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
004659   B6.Cg-Tg(TIE2GFP)287Sato/1J
007921   B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J
006000   B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
004509   B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
006417   B6.FVB-Tg(Npy-hrGFP)1Lowl/J
005738   B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J
008126   B6.NOD-Tg(Cd4-EGFP)1Lt/J
004077   B6;129-Gt(ROSA)26Sortm2Sho/J
006667   B6;129P2-Omptm3Mom/MomJ
008605   B6;C3-Tg(CAG-DsRed,-EGFP)5Gae/J
008080   B6;C3-Tg(CAG-SAC/EGFP)35Rang/J
004966   B6;CBA-Tg(Acrv1-EGFP)2727Redd/J
004654   B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J
005621   B6;D2-Tg(S100B-EGFP)1Wjt/J
004690   B6;FVB-Tg(Pcp2-EGFP)2Yuza/J
006147   B6;FVB-Tg(Sfpi1,-EGFP)7Dgt/J
006043   B6;SJL-Tg(Oxt/EGFP)AI03Wsy/J
004512   C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
006567   C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ
003291   C57BL/6-Tg(CAG-EGFP)1Osb/J
005070   C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J
004353   C57BL/6-Tg(UBC-GFP)30Scha/J
005706   C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
007567   C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
008234   CB6-Tg(CAG-EGFP/CETN2)3-4Jgg/J
007677   CB6-Tg(Gad1-EGFP)G42Zjh/J
007075   CByJ.B6-Tg(CAG-EGFP)1Osb/J
007076   CByJ.B6-Tg(UBC-GFP)30Scha/J
003718   FVB-Tg(GadGFP)45704Swn/J
005515   FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
006421   FVB-Tg(Pomc1-hrGFP)1Lowl/J
005688   FVB-Tg(Rag2-EGFP)1Mnz/J
003516   FVB.Cg-Tg(CAG-EGFP)B5Nagy/J
008200   FVB/N-Tg(CAG-EGFP,-ALPP)2.6Ggc/J
003257   FVB/N-Tg(GFAPGFP)14Mes/J
008173   NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ
005076   NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ
008547   NOD.FVB-Tg(ITGAM-DTR/EGFP)34Lan/JdkJ
008549   NOD.FVB-Tg(Itgax-DTR/EGFP)57Lan/JdkJ
005082   NOD/ShiLt-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ
005334   NOD/ShiLt-Tg(Cd4-EGFP)1Lt/J
008694   NOD/ShiLt-Tg(Foxp3-EGFP/cre)1Jbs/J
005282   NOD/ShiLtJ-Tg(Ins1-EGFP/GH1)14Hara/HaraJ
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
006741   STOCK Olfr160tm1Mom Tg(Olfr151,taulacZ)BMom/MomJ
006770   STOCK Rag1tm1Mom Tg(TIE2GFP)287Sato/J
006570   STOCK Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj Tg(SMN2)89Ahmb/J
006850   STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
006876   STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J
003920   STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
003115   STOCK Tg(CAG-EGFP)B5Nagy/J
003116   STOCK Tg(CAG-EGFP)D4Nagy/J
005105   STOCK Tg(Chx10-EGFP/cre-ALPP)2Clc/J
005854   STOCK Tg(Cp-EGFP)25Gaia/J
006334   STOCK Tg(Gad1-EGFP)94Agmo/J
006340   STOCK Tg(Gad1-EGFP)98Agmo/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
005418   STOCK Tg(HIST1H2BB/EGFP)1Pa/J
003658   STOCK Tg(TIE2GFP)287Sato/J
006129   STOCK Tg(Zp3-EGFP)1Dean/J
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005699   STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
View Strains carrying other alleles of GFP     (92 strains)

Strains carrying other alleles of Sry
007250   C57BL/6JEi-Chr YAKR/J/Ei
001543   C57BL/6JEi-Chr YCB/EiJ
View Strains carrying other alleles of Sry     (2 strains)

View Strains carrying other alleles of Tyr     (46 strains)

Additional Web Information

Congenic Nomenclature
Fluorescent Proteins/lacZ Systems

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Tg(Sry-EGFP)92Ei related

Developmental Biology Research

Neurobiology Research

Research Tools
Fluorescent Proteins
Reproductive Biology Research

Currently there is no phenotype information for this strain.

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(Sry-EGFP)92Ei
Allele Name transgene insertion 92, Eva M Eicher
Allele Type Transgenic (Reporter)
Common Name(s) Tg92Ei;
Strain of OriginC57BL/6J
Site of ExpressionFluorescence is detected in pre-sertoli and pre-granulosa cells of the genital ridge, expression is stronger in homozygotes. Expression is also detected in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males but not females.
Expressed Gene GFP, Green Fluorescent Protein, jellyfish
Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source.
Promoter Sry, sex determining region of Chr Y, mouse, laboratory
Molecular Note The transgenic vector was composed of the Sry promoter region from bp 542-8304 inserted upstream of the EGFP-1 coding region. The construct contains two SV40 early mRNA polyadenylation signal in place of the endogenous Sry 3' UTR. Expression of EGFP indicates that the spatial expression of EGFP mRNA and protein is similar to endogenous Sry . [MGI Ref ID J:73280]
 
Allele Symbol Tg(Tyr)3412ARpw
Allele Name transgene insertion 3412A, Richard P Woychick
Allele Type Transgenic (random, expressed)
Site of ExpressionFluorescence is detected in pre-sertoli and pre-granulosa cells of the genital ridge, expression is stronger in homozygotes. Expression is also detected in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males but not females.
Expressed Gene Tyr, tyrosinase, mouse, laboratory
Promoter Tyr, tyrosinase, mouse, laboratory

Genotyping

Genotyping Information

Genotyping Protocols

Fluorescent Proteins (Generic GFP), STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Albrecht KH; Eicher EM. 2001. Evidence that sry is expressed in pre-sertoli cells and sertoli and granulosa cells have a common precursor. Dev Biol 240(1):92-107. [PubMed: 11784049]  [MGI Ref ID J:73280]

Dewing P; Chiang CW; Sinchak K; Sim H; Fernagut PO; Kelly S; Chesselet MF; Micevych PE; Albrecht KH; Harley VR; Vilain E. 2006. Direct regulation of adult brain function by the male-specific factor SRY. Curr Biol 16(4):415-20. [PubMed: 16488877]  [MGI Ref ID J:123875]

Additional References

Tg(Tyr)3412ARpw related

Woychik RP. 2007. Tyrosinase Minigene insertion in Chromosome X MGI Direct Data Submission :.  [MGI Ref ID J:120431]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryCarriers of Tg(Tyr)3412ARpw on this albino background are pigmented while non-carriers are albino. This strain is maintained at The Jackson Laboratory by sibling breeding mice homozygous for Tg(Sry-EGFP)92Ei and segregating for Tg(Tyr)3412ARpw. A female or male pigmented mouse, therefore an obligate carrier of Tg(Tyr)3412ARpw, is bred with an albino non-carrier. For experimental purposes a pigmented male bred with an albino female will generate albino male and pigmented female offspring.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

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