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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation N10F?pN1 Donating Investigator Eva Eicher, The Jackson Laboratory Appearance
albino female
Related Genotype: A/A Tyrc/Tyrc +/+albino male
Related Genotype: A/A Tyrc/Tyrc +/Ymottled gray-brown female
Related Genotype: A/A Tyrc/Tyrc Tg(Tyr)3412ARpw/+dark gray-brown male
Related Genotype: A/A Tyrc/Tyrc Tg(Tyr)3412ARpw/YView images of coat colors for this strain.
Description
On an albino background Tg(Tyr)3412ARpw permits the identification of gender as early as embryonic day 10.5. This strain is segregating for Tg(Tyr)3412ARpw and homozygous for Tyrc-2J so the individuals not carrying Tg(Tyr3412)ARpw are albino. Because Tg(Tyr)3412ARpw inserted into the X Chromosome, breeding a carrier male with a noncarrier (wild-type) female results in embryos in which all XX individuals develop eye pigment, due to the Tg(Tyr)3412ARpw inherited from their father, while all XY individuals have non-pigmented eyes, having inherited a wild-type X Chromosome from their mother.This strain is also homozygous for Tg(SryEGFP)92Ei. This reporter transgene consists of a 5' regulatory segment of the Sry gene driving EGFP. This transgene is expressed in the pre-support cell lineage (pre-sertoli and pre-granulosa cells) of the fetal genital ridge (Albrecht and Eicher, 2001) and in discrete areas the adult male but not female brain (Dewing et al., 2006). Specifically, Sry has been found expressed in tyrosine hydroxylase expressing neurons in all regions of the male substantial nigra, most strongly in the pars compacta. The expression of EGFP from this transgene is stronger in homozygotes than in heterozygotes. This strain is maintained homozygous for Tg(SryEGFP)92Ei.
Development
Tg(Tyr)3412ARpw was generated by microinjection into FVB/N fertilized eggs and integrated into the X Chromosome. Tg(Sry-EGFP)92Ei was generated by microinjection into C57BL/6J fertilized eggs. Tg(Sry-EGFP)92Ei was later backcrossed onto the FVB/NJ background, starting by crossing an FVB/NJ female with a C57BL/6J-Tg(Sry-EGFP)92Ei carrier male. The N2 backcross offspring were typed for MIT markers and Tg(Sry-EGFP)92Ei was determined to map to a position of Chromosome 5 between D5Mit255 and D5Mit197. An N2 mouse carrying Tg(Sry-EGFP)92Ei and B6 alleles of these D5Mit markers was selected to continue the backcross. At the N9 backcross generation a female carrying Tg(Sry-EGFP)92Ei and the two flanking B6-derived D5Mit loci was mated to an FVB/N-Tg(Tyr)3412ARpw male. The resulting N10 offspring were typed for Tg(Sry-EGFP)92Ei and the B6 alleles of D5Mit255 and D5Mit197 and carriers were intercrossed and their N10F2 offspring were typed to identify breeders homozygous for the B6 allele of D5Mit255 and D5Mit197. Thereafter this strain has been maintained homozygous for Tg(Sry-EGFP)92Ei and segregating for Tg(Tyr)3412ARpw. The pigment conferred to carriers of Tg(Tyr)3412ARpw on this otherwise albino background makes breeder selection easy since one need only pair up one albino mouse with a pigmented sibling.
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (170 strains)
007484 B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ 007265 C57BL/6-Tg(Sry-EGFP)92Ei Chr YAKR/J/EiJ 007264 C57BL/6-Tg(Sry-EGFP)92Ei Tg(Sry)4Ei Chr YPOS/EiJ 003927 C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ
007484 B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
007250 C57BL/6JEi-Chr YAKR/J/Ei 001543 C57BL/6JEi-Chr YCB/EiJ
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
Currently there is no phenotype information for this strain.This mouse can be used to support research in many areas including:Tg(Sry-EGFP)92Ei related
Developmental Biology Research
Neurobiology Research
Research Tools
Fluorescent Proteins
Reproductive Biology Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Sry-EGFP)92Ei | ||
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| Allele Name | transgene insertion 92, Eva M Eicher | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Tg92Ei; | ||
| Strain of Origin | C57BL/6J | ||
| Site of Expression | Fluorescence is detected in pre-sertoli and pre-granulosa cells of the genital ridge, expression is stronger in homozygotes. Expression is also detected in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males but not females. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Sry, sex determining region of Chr Y, mouse, laboratory | ||
| Molecular Note | The transgenic vector was composed of the Sry promoter region from bp 542-8304 inserted upstream of the EGFP-1 coding region. The construct contains two SV40 early mRNA polyadenylation signal in place of the endogenous Sry 3' UTR. Expression of EGFP indicates that the spatial expression of EGFP mRNA and protein is similar to endogenous Sry . [MGI Ref ID J:73280] | ||
| Allele Symbol | Tg(Tyr)3412ARpw | ||
| Allele Name | transgene insertion 3412A, Richard P Woychick | ||
| Allele Type | Transgenic (random, expressed) | ||
| Site of Expression | Fluorescence is detected in pre-sertoli and pre-granulosa cells of the genital ridge, expression is stronger in homozygotes. Expression is also detected in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males but not females. | ||
| Expressed Gene | Tyr, tyrosinase, mouse, laboratory | ||
| Promoter | Tyr, tyrosinase, mouse, laboratory | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Albrecht KH; Eicher EM. 2001. Evidence that sry is expressed in pre-sertoli cells and sertoli and granulosa cells have a common precursor. Dev Biol 240(1):92-107. [PubMed: 11784049] [MGI Ref ID J:73280]
Dewing P; Chiang CW; Sinchak K; Sim H; Fernagut PO; Kelly S; Chesselet MF; Micevych PE; Albrecht KH; Harley VR; Vilain E. 2006. Direct regulation of adult brain function by the male-specific factor SRY. Curr Biol 16(4):415-20. [PubMed: 16488877] [MGI Ref ID J:123875]
Additional References
Tg(Tyr)3412ARpw relatedWoychik RP. 2007. Tyrosinase Minigene insertion in Chromosome X MGI Direct Data Submission :. [MGI Ref ID J:120431]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry Carriers of Tg(Tyr)3412ARpw on this albino background are pigmented while non-carriers are albino. This strain is maintained at The Jackson Laboratory by sibling breeding mice homozygous for Tg(Sry-EGFP)92Ei and segregating for Tg(Tyr)3412ARpw. A female or male pigmented mouse, therefore an obligate carrier of Tg(Tyr)3412ARpw, is bred with an albino non-carrier. For experimental purposes a pigmented male bred with an albino female will generate albino male and pigmented female offspring.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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General Terms and Conditions
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