Strain Name: |
B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ |
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Stock Number: |
007484 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | GFP; Sry; Tyr; Tyrc-2J; Tg(Sry-EGFP)92Ei; Tg(Tyr)3412ARpw; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Spontaneous Mutation Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Eva Eicher, The Jackson Laboratory Appearance
albino female
Related Genotype: a/a Tyrc-2J/Tyrc-2J +/+albino male
Related Genotype: a/a Tyrc-2J/Tyrc-2J +/Ymottled gray-black female
Related Genotype: a/a Tyrc-2J/Tyrc-2J Tg(Tyr)3412ARpw/+dark gray-black male
Related Genotype: a/a Tyrc-2J/Tyrc-2J Tg(Tyr)3412ARpw/YView images of coat colors for this strain.
Strain Description
On an albino background Tg(Tyr)3412ARpw permits the identification of gender as early as embryonic day 10.5. This strain is segregating for Tg(Tyr)3412ARpw and homozygous for Tyrc-2J so the individuals not carrying Tg(Tyr3412)ARpw are albino. Because Tg(Tyr)3412ARpw inserted into the X Chromosome, breeding a carrier male with a noncarrier (wild-type) female results in embryos in which all XX individuals develop eye pigment, due to the Tg(Tyr)3412ARpw inherited from their father, while all XY individuals have non-pigmented eyes, having inherited a wild-type X Chromosome from their mother.This strain is also homozygous for Tg(SryEGFP)92Ei. This reporter transgene consists of a 5 prime regulatory segment of the Sry gene driving EGFP. This transgene is expressed in the pre-support cell lineage (pre-sertoli and pre-granulosa cells) of the fetal genital ridge (Albrecht and Eicher, 2001) and in discrete areas the adult male but not female brain (Dewing et al., 2006). Specifically, Sry has been found expressed in tyrosine hydroxylase expressing neurons in all regions of the male substantial nigra, most strongly in the pars compacta. The expression of EGFP from this transgene is stronger in homozygotes than in heterozygotes. This strain is maintained homozygous for Tg(SryEGFP)92Ei.
Strain Development
Tg(Tyr)3412ARpw was generated by microinjection into FVB/N fertilized eggs and integrated into the X Chromosome. Tg(Tyr)3412ARpw was backcrossed onto the B6(Cg)-Tyrc-2J/J albino background, where carriers of the transgene can be detected by coat color. Tg(Sry-EGFP)92Ei was generated by microinjection into C57BL/6J fertilized eggs. C57BL/6J-Tg(Sry-EGFP)92Ei was bred with the C57BL/6J congenic strain bearing both Tyrc-2J and Tg(Tyr)3412ARpw and the resulting offspring were intercrossed to generate this strain, which is homozygous for Tyrc-2J, Tg(Sry-EGFP)92Ei, and segregating for Tg(Tyr)3412ARpw.
Gene & Allele Details
| Allele Symbol | Tg(Sry-EGFP)92Ei | ||
|---|---|---|---|
| Allele Name | transgene insertion 92, Eva M Eicher | ||
| Common Name(s) | Tg92Ei; | ||
| Strain of Origin | C57BL/6J | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Sry, sex determining region of Chr Y, mouse, laboratory | ||
| Molecular Note | The transgenic vector was composed of the Sry promoter region from bp 542-8304 inserted upstream of the EGFP-1 coding region. The construct contains two SV40 early mRNA polyadenylation signal in place of the endogenous Sry 3' UTR. Expression of EGFP indicates that the spatial expression of EGFP mRNA and protein is similar to endogenous Sry . [J:73280] | ||
| Allele Symbol | Tg(Tyr)3412ARpw | ||
| Allele Name | transgene insertion 3412A, Richard P Woychick | ||
| Expressed Gene | Tyr, tyrosinase, mouse, laboratory | ||
| Promoter | Tyr, tyrosinase, mouse, laboratory | ||
| Allele Symbol | Tyrc-2J | ||
| Allele Name | albino 2 Jackson | ||
| Strain of Origin | B6.Cg-Tyrp1 Hps1 | ||
| Molecular Note | This mutation has a G to T base change at nucleotide 291 resulting in an amino acid change from arginine to leucine at residue 77 which lies in the highly conserved DDRE sequence. Nucleotide 291 is at the alternative 5' splice donor site for exon 1 and this allele does not produce the 1a or 1b subset of tyrosinase transcripts but does produce a significant increase in 1c and 1d transcripts. Western blots of homozygous mutant skin extracts demostrate the nearly complete absence of the broad 76-84 kDa bandof glycosylated wild-type tyrosinase. No tyrosinase activity was found in hairbulb extracts from homozygous mice. [J:36008] [J:6611] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Tyrc-2J
Colony Maintenance
| Breeding & Husbandry | Carriers of Tg(Tyr)3412ARpw on this Tyrc-2J homozygous background are pigmented while non-carriers are albino. This strain is maintained at The Jackson Laboratory by sibling breeding mice homozygous for Tg(Sry-EGFP)92Ei and Tyrc-2J and segregating for Tg(Tyr)3412ARpw. A female or male pigmented mouse, therefore an obligate carrier of Tg(Tyr)3412ARpw, is bred with an albino non-carrier. For experimental purposes a pigmented male bred with an albino female will generate albino male and pigmented female offspring. |
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Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (138 strains)
000058 B6(Cg)-Tyrc-2J/J
007265 C57BL/6-Tg(Sry-EGFP)92Ei Chr YAKR/J/EiJ 007264 C57BL/6-Tg(Sry-EGFP)92Ei Tg(Sry)4Ei Chr YPOS/EiJ 003927 C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ 007483 FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
007483 FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
007250 C57BL/6JEi-Chr YAKR/J/Ei 001543 C57BL/6JEi-Chr YCB/EiJ
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number A2A
Research Applications
This mouse can be used to support research in many areas including:
Tyrc-2J relatedResearch Tools
Developmental Biology Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers)
Reproductive Biology Research
Tg(Sry-EGFP)92Ei relatedDermatology Research
Color and White Spotting Defects
Mouse/Human Gene Homologs
albinism, tyrosine negative
Sensorineural Research
Retinal Degeneration
Developmental Biology Research
Neurobiology Research
Research Tools
Fluorescent Proteins
Reproductive Biology Research
References
Selected Reference(s)
Additional ReferencesAlbrecht KH; Eicher EM. 2001. Evidence that sry is expressed in pre-sertoli cells and sertoli and granulosa cells have a common precursor. Dev Biol 240(1):92-107. [PubMed: 11784049] [J:73280]
Dewing P; Chiang CW; Sinchak K; Sim H; Fernagut PO; Kelly S; Chesselet MF; Micevych PE; Albrecht KH; Harley VR; Vilain E. 2006. Direct regulation of adult brain function by the male-specific factor SRY. Curr Biol 16(4):415-20. [PubMed: 16488877] [J:123875]
Price and Supply Information
| Strain Name: | B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ |
| Stock Number: | 007484 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $124.10 | Homozygous for Tyrc-2J, Hemizygous for Tg(Tyr)3412ARpw, Homozygous for Tg(Sry-EGFP)92Ei | |||
| Individual Mouse Price | $124.10 | Homozygous for Tyrc-2J, Noncarrier, Homozygous for Tg(Sry-EGFP)92Ei | |||
| Pair | $248.20 | Homozygous for Tyrc-2J, Hemizygous for Tg(Tyr)3412ARpw, Homozygous for Tg(Sry-EGFP)92Ei x Homozygous for Tyrc-2J, Noncarrier, Homozygous for Tg(Sry-EGFP)92Ei | |||
| Pair | $248.20 | Homozygous for Tyrc-2J, Noncarrier, Homozygous for Tg(Sry-EGFP)92Ei x Homozygous for Tyrc-2J, Hemizygous for Tg(Tyr)3412ARpw, Homozygous for Tg(Sry-EGFP)92Ei | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Mouse Mutant Resource collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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