Strain Name:

B6SJL-Bicc1tm1Emdr/J

Stock Number:

007555

Availability:

Repository-Cryopreserved

Use Restrictions Apply, see Purchasing Information

Description

Strain Information

Type Mutant Stock; Targeted Mutation;
Specieslaboratory mouse
 
Donating Investigator Edward De Robertis,   University of California, Los Angeles

Description
Homozygous mutants survive a few weeks after birth, but display polycystic kidney disease. LacZ expression was observed in the Henson's node at embryonic day 7.5 and in the endoderm of the gut at embryonic day 8.5

Development
A targeting vector was used to place an in-frame LacZ reporter gene in the first exon of the gene at the initiator methionine and insert a neomycin resistance cassette downstream of the LacZ cassette. The targeting vector was introduced to LW1 129S4/SvJae-derived embryonic stem (ES) cells. This line has been backcrossed to B6SJLF1/J for 14 generations by the donating laboratory.

Phenotype

Phenotype Information

Related Disease (OMIM) Terms

Polycystic Kidney Disease, Autosomal Recessive; ARPKD

Mammalian Phenotype Terms assigned by genotype

Bicc1tm1Emdr/Bicc1tm1Emdr

        B6SJL-Bicc1tm1Emdr
  • lethality-postnatal
  • postnatal lethality (MGI Ref ID J:121683)
    • mice survive a for a few weeks after birth
  • renal/urinary system phenotype
  • polycystic kidney (MGI Ref ID J:121683)
    • homozygotes have polycystic kidneys

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Postnatal Mortality

Internal/Organ Research
Kidney Defects (polycystic kidney disease)

Genes & Alleles

Gene & Allele Information

Allele Symbol Bicc1tm1Emdr
Allele Name targeted mutation 1, E M de Robertis
Common Name(s) BicC;
Mutation Made By Edward De Robertis,   University of California, Los Angeles
Strain of Origin129S4/SvJae
ES Cell Line NameLW1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Bicc1, bicaudal C homolog 1 (Drosophila)
Chromosome 10
Gene Common Name(s) Bic-C; bpk; jcpk; juvenile congenital polycystic kidney disease; polycystic kidney mutation of BALB/c origin;
Molecular Note A targeting vector was used to place an in-frame LacZ reporter gene in the first exon of the gene at the initiator methionine and insert a neomycin resistance cassette downstream of the LacZ cassette. LacZ expression was observed in the Henson's node at embryonic day 7.5 and in the endoderm of the gut at embryonic day 8.5 [MGI Ref ID J:121683]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

de Robertis EM. 2007. A targeted mutation of Bicc1 MGI Direct Data Submission :.  [MGI Ref ID J:121683]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as heterozygotes. Homozygotes survive only a few weeks after birth.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations             View   International   Pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.

Pricing for International shipping destinations             View   USA, Canada and Mexico   Pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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