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Genes & Alleles

Oprd1;   Oprd1tm1Kff;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote         (Female x Male) Species laboratory mouse Donating Investigator Brigitte Kieffer,   Université Louis Pasteur Strasbour Generation N13+N1F1 (04-APR-08)

Strain Description
Mice homozygous for this delta-opioid receptor mutant allele (DOR-) are viable and fertile. DOR-selective ligand binding is absent in vitro on brain membranes from homozygous mice. Unlike mutant mice deficient in kappa- or mu-opioid receptors (Stock No. 007558 or 007559, respectively), homozygous DOR- mutants exhibit increased anxiety and depressive-like behavior but no alteration of spontaneous pain perception. Homozygous DOR- mice also have increased ethanol self-administration and increased inflammatory pain. These DOR mutant mice may be useful in studying the biological activity of opioids andanalgesics, mechanical nociception, inflammatory pain, and emotional disorders.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. These mutant mice were originally published on a mixed genetic background, and the donating investigator reports that increased anxiety may not be observable on this congenic background. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Strain Development
A targeting vector was designed to replace the entire first exon (amino terminal region and ATG translation initiation codon) of the targeted gene with a PGK-neo cassette. The donating investigator reports that the construct was electroporated into 129S2/SvPac-derived embryonic stem (ES) cells, and correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric males were bred with C57BL/6 females. These delta-opioid receptor (DOR) mutant mice were backcrossed to C57BL/6J inbred mice for at least 12 generations prior to arrival at The Jackson Laboratory. The Y chromosome may not have been fixed for the C57BL/6J genetic background.

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Oprd1tm1Kff/Oprd1tm1Kff         involves: 129/Sv * C57BL/6 behavior/neurological phenotype decreased coping response (MGI Ref ID J:62551) strong increase in time of immobility in forced-swim test hyperactivity (MGI Ref ID J:62551) increased anxiety-related response (MGI Ref ID J:62551) tested in elevated plus-maze and light-dark box

Gene & Allele Details

Allele Symbol		Oprd1tm1Kff
Allele Name		targeted mutation 1, Brigitte L Kieffer
Common Name(s)		DOR-; Oprd1-/-;
Mutation Made By		Brigitte Kieffer,   Université Louis Pasteur Strasbour
Strain of Origin		129/Sv
Gene Symbol and Name		Oprd1, opioid receptor, delta 1
Chromosome		4
Gene Common Name(s)		DOR-1; Nbor; OPRD; mDOR; neuroblastoma opiate receptor;
Molecular Note		Exon 1, which encodes the translational start codon, was replaced by a neomycin selection cassette inserted by homologous recombination. A radiolabeled binding assay demonstrated complete functional ablation in homozygous mutant mice. [MGI Ref ID J:62551]

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Genotyping Protocols

Oprd1^tm1Kff

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, heterozygous or homozygous mice may be bred.
Diet Information	LabDiet® 5K52/5K67

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Immunology and Inflammation Research
Inflammation

Neurobiology Research
Behavioral and Learning Defects (genes regulating preferences to alcohol and stress)
Behavioral and Learning Defects (genes regulating preferences to alcohol)
Behavioral and Learning Defects (high anxiety)
Receptor Defects (opiod)

Research Tools
Immunology and Inflammation Research
Sensorineural Research
Toxicology Research (drug/compound testing)

Sensorineural Research
Nociception

References

Selected Reference(s)

Filliol D; Ghozland S; Chluba J; Martin M; Matthes HW; Simonin F; Befort K; Gaveriaux-Ruff C; Dierich A; LeMeur M; Valverde O; Maldonado R; Kieffer BL. 2000. Mice deficient for delta- and mu-opioid receptors exhibit opposing alterations of emotional responses. Nat Genet 25(2):195-200. [PubMed: 10835636]  [MGI Ref ID J:62551]

Martin M; Matifas A; Maldonado R; Kieffer BL. 2003. Acute antinociceptive responses in single and combinatorial opioid receptor knockout mice: distinct mu, delta and kappa tones. Eur J Neurosci 17(4):701-8. [PubMed: 12603260]  [MGI Ref ID J:108005]

Additional References

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Price and Supply Information

Strain Name:	B6.129S2-Oprd1tm1Kff/J
Stock Number:	007557

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities may require a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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