Strain Name: |
B6.129S2-Oprk1tm1Kff/J |
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Stock Number: |
007558 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Oprk1; Oprk1tm1Kff; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Brigitte Kieffer, Université Louis Pasteur Strasbour Generation N12+N1F1 (06-AUG-08) Strain Description
Mice homozygous for this kappa-opioid receptor mutant allele (KOR-) are viable and fertile. KOR-selective ligand binding is absent on brain membranes from homozygous mice. In contrast to mutant mice deficient of delta- or mu-opioid receptors (Stock No. 007557 or 007559), KOR- homozygotes have normal mechanical pain thresholds, but greatly increased sensitivity to chemical visceral pain. KOR- homozygotes also exhibit normal spontaneous stress responses (unlike delta-opioid receptor null mice). Homozygous KOR- mice also show decreased ethanol self-administration and decreased THC-conditioned place aversion. These KOR mutant mice may be useful in studying biological activity of opioids, analgesics, spinally mediated thermal nociception and chemical visceral pain thresholds.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. These mutant mice were originally published on a mixed genetic background. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A targeting vector was designed to replace a portion of the first exon (containing the translation initiation codon) of the targeted gene with a PGK-neo cassette. The construct was electroporated into 129S2/SvPas-derived P1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric males were bred with C57BL/6 females. These kappa-opioid receptor (KOR) mutant mice were backcrossed to C57BL/6J inbred mice for at least 12 generations prior to arrival at The Jackson Laboratory. The Y chromosome may not have been fixed to the C57BL/6J genetic background.
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Oprk1tm1Kff | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Brigitte L Kieffer | ||
| Common Name(s) | KOR -; KOR(-); Oprk1-; | ||
| Mutation Made By | Brigitte Kieffer, Université Louis Pasteur Strasbour | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | P1 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | Oprk1, opioid receptor, kappa 1 | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | KOR; KOR-1; OPRK; Oprk2; R21; opioid receptor, kappa 2; | ||
| Molecular Note | Exon 1 was disrupted by the insertion of a neomycin selection cassette. The insertion deleted sequence encoding amino acids 1 through 79. A radiolabeled binding assay demonstrated a lack of endogenous function. [MGI Ref ID J:46089] | ||
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Breeding & Husbandry | When maintaining a live colony, heterozygous or homozygous mice may be bred. |
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| Diet Information | LabDiet® 5K52/5K67 |
Congenic Nomenclature
Room Number AX11
Neurobiology Research
Behavioral and Learning Defects (genes regulating preferences to alcohol)
Receptor Defects (opiod)
Research Tools
Sensorineural Research
Toxicology Research (drug/compound testing)
Sensorineural Research
Nociception
Selected Reference(s)
Additional ReferencesMartin M; Matifas A; Maldonado R; Kieffer BL. 2003. Acute antinociceptive responses in single and combinatorial opioid receptor knockout mice: distinct mu, delta and kappa tones. Eur J Neurosci 17(4):701-8. [PubMed: 12603260] [MGI Ref ID J:108005]
Simonin F; Valverde O; Smadja C; Slowe S; Kitchen I; Dierich A; Le Meur M; Roques BP; Maldonado R; Kieffer BL. 1998. Disruption of the kappa-opioid receptor gene in mice enhances sensitivity to chemical visceral pain, impairs pharmacological actions of the selective kappa-agonist U-50,488H and attenuates morphine withdrawal. EMBO J 17(4):886-97. [PubMed: 9463367] [MGI Ref ID J:46089]
| Strain Name: | B6.129S2-Oprk1tm1Kff/J |
| Stock Number: | 007558 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities may require a license prior to shipping.
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