Strain Name: |
B6.129S2-Oprm1tm1Kff/J |
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Stock Number: |
007559 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Oprm1; Oprm1tm1Kff; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Brigitte Kieffer, Université Louis Pasteur Strasbour Strain Description
Mice homozygous for this mu-opioid receptor mutant allele (MOR-) are viable and fertile. MOR-selective ligand binding is absent on brain membranes from homozygous mice. Homozygous MOR- mice exhibit a lack of morphine analgesia, reward, and withdrawal. This is accompanied by decreased mechanical, thermal, and chemical pain thresholds. Homozygous MOR- mice also show decreased ethanol self-administration and decreased THC-conditioned place aversion. In contrast to mutant mice deficient of delta- or kappa-opioid receptors (Stock No. 007557 or 007558, respectively), MOR- homozygotes exhibit hypolocomotive spontaneous stress responses. Indeed, the reduced anxiety and depressive-like behavior observed in MOR- mutants is in stark contrast to kappa-opioid receptor deficient mice. These MOR mutant mice may be useful in studying the biological activity of opioids, analgesics, and responses to mechanical, chemical and thermal nociception at a supraspinal level.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. These mutant mice were originally published on a mixed genetic background. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A targeting vector was designed to insert a PGK-neo cassette into exon 2 of the targeted gene. The construct was electroporated into 129S2/SvPas-derived P1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric mice were bred with C57BL/6 mice. These mu-opioid receptor (MOR) mutant mice were backcrossed to C57BL/6J inbred mice for at least 12 generations prior to arrival at The Jackson Laboratory. The Y chromosome may not have been fixed to the C57BL/6J genetic background.
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Oprm1tm1Kff | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Brigitte L Kieffer | ||
| Common Name(s) | MOR-; | ||
| Mutation Made By | Brigitte Kieffer, Université Louis Pasteur Strasbour | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | P1 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | Oprm1, opioid receptor, mu 1 | ||
| Chromosome | 10 | ||
| Gene Common Name(s) | KIAA0403; MOP receptor; MOP-R; MOR; MOR-1; MOR1; MORA; OPRM; muOR; | ||
| Molecular Note | A neomycin selection cassette was inserted into exon 2. Binding assays on brain of homozygous animals confirmed that no functional protein was made from this allele. [MGI Ref ID J:36241] | ||
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Oprm1tm1Kff
| Breeding & Husbandry | When maintaining a live colony, heterozygous or homozygous mice may be bred. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Congenic Nomenclature
Room Number AX11
Neurobiology Research
Behavioral and Learning Defects (genes regulating preferences to alcohol and stress)
Behavioral and Learning Defects (genes regulating preferences to alcohol)
Behavioral and Learning Defects (mu opioid receptor deficiency)
Receptor Defects (opiod)
Research Tools
Sensorineural Research
Toxicology Research (drug/compound testing)
Sensorineural Research
Nociception
Selected Reference(s)
Additional ReferencesMartin M; Matifas A; Maldonado R; Kieffer BL. 2003. Acute antinociceptive responses in single and combinatorial opioid receptor knockout mice: distinct mu, delta and kappa tones. Eur J Neurosci 17(4):701-8. [PubMed: 12603260] [MGI Ref ID J:108005]
Matthes HW; Maldonado R; Simonin F; Valverde O; Slowe S; Kitchen I; Befort K; Dierich A; Le Meur M; Dolle P; Tzavara E; Hanoune J; Roques BP; Kieffer BL. 1996. Loss of morphine-induced analgesia, reward effect and withdrawal symptoms in mice lacking the mu-opioid-receptor gene [see comments] Nature 383(6603):819-23. [PubMed: 8893006] [MGI Ref ID J:36241]
| Strain Name: | B6.129S2-Oprm1tm1Kff/J |
| Stock Number: | 007559 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities may require a license prior to shipping.
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