Strain Name: |
STOCK Sim1tm1.2Az/J |
|---|---|
Stock Number: |
007570 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Sim1; Sim1tm1.2Az; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Andrew Zinn, UT Southwestern Medical School Generation ?+F1 (27-FEB-08) Strain Description
Mice homozygous for this "Sim1-floxed exon 1" (2-loxP) conditional allele are viable and fertile, with loxP sites flanking the translation start site and the first 17 amino acids of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for the basic domain of SIM1) deleted in the cre-expressing tissue(s). These "Sim1-floxed exon 1" (2-loxP) mice may be useful in generating conditional mutations for studying basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS) transcription factors, central nervous system development, early-onset/hyperphagic obesity, and regulation of appetite and energy balance.Strain Development
A targeting vector was designed to place a loxP site just upstream of the ATG translational start site and a loxP-flanked PGK-Neo cassette downstream of the same exon of the targeted gene. In the primary publication, a theoretical first exon preceded the exon containing the Sim1 ATG start site. The donating investigator reports that the theoretical first exon is no longer considered to represent a functional exon). The targeting construct was electroporated into 129X1/SvJ-derived RW-4 embryonic stem (ES) cells. Chimeric mice were bred to C57BL/6 mice to generate heterozygous "three-loxP" mice. Next, the neomycin selection cassette was excised by crossing to FVB transgenic mice with widespread Cre recombinase expression (see Stock No. 003314). The resulting offspring harboring the "floxed exon 1" (2-loxP) conditional allele (loxP-flanked exon 1 with PGK-neo removed) were intercrossed and then maintained as a homozygous colony prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to C57BL/6J for at least one generation to establish the colony.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Sim1tm1.2Az | ||
|---|---|---|---|
| Allele Name | targeted mutation 1.2, Andrew R Zinn | ||
| Common Name(s) | 2-loxP; Sim1-floxed exon 1 (PGK-Neo excised); | ||
| Mutation Made By | Ying-Hue Lee, Inst. of Mol. Bio., Academia Sinica | ||
| Strain of Origin | 129X1/SvJ | ||
| ES Cell Line Name | RW-4 | ||
| ES Cell Line Strain | 129X1/SvJ | ||
| Gene Symbol and Name | Sim1, single-minded homolog 1 (Drosophila) | ||
| Chromosome | 10 | ||
| Gene Common Name(s) | MGC189161; bHLHe14; | ||
| Molecular Note | A targeting vector was designed to place a loxP site just upstream of the ATG translational start site and a loxP-flanked PGK-Neo cassette downstream of the same exon of the targeted gene. The construct was injected into RW-4 ES cells. Chimeric mice werebred to C57BL/6 mice, and the neomycin selection cassette was excised from these mice by crossing to FVB transgenic mice with widespread Cre recombinase expression. [MGI Ref ID J:95410] | ||
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Sim1tm1.2Az
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, homozygous mice may be bred together. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Sim1
006451 B6.FVB(129X1)-Tg(Sim1-cre)1Lowl/J 006395 STOCK Tg(Sim1-cre)1Lowl/J View Strains carrying other alleles of Sim1 (2 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
Neurobiology Research
Cre-lox System (loxP-flanked Sequences)
Research Tools
Cell Biology Research
Cre-lox System (loxP-flanked Sequences)
Diabetes and Obesity Research (loxP)
Metabolism Research
References
Selected Reference(s)
Holder JL Jr; Zhang L; Kublaoui BM; DiLeone RJ; Oz OK; Bair CH; Lee YH; Zinn AR. 2004. Sim1 gene dosage modulates the homeostatic feeding response to increased dietary fat in mice. Am J Physiol Endocrinol Metab 287(1):E105-13. [PubMed: 14982752] [MGI Ref ID J:95410]
Price and Supply Information
| Strain Name: | STOCK Sim1tm1.2Az/J |
| Stock Number: | 007570 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by non-profits requires an MTA and for-profit entities require a license.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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