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| These RorcγtGFP mutant mice may be useful in studying immune system homeostasis, T cell repertoire selection, CD4/CD8 double positive (CD4+/CD8+) thymocyte survival, lymphoid organogenesis, proinflammatory T-helper cell (Th17) development, mucosal immunology, and the role of inflammatory disease in autoimmunity and cancer progression. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
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Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Homozygote (Female x Male) 26-JAN-11 Species laboratory mouse Generation N6+N2F1 (28-DEC-12)
Generation DefinitionsDonating Investigator Dr. Dan R. Littman, New York University Medical Center Description
Mice homozygous for this Rorc(γtGFP (or RORγt)GFP) mutant allele are viable and fertile. While Rorcγ mRNA is detected in liver in Rorc(γ)tGFP homozygotes, mRNA and protein for the thymus-specific isoform (Rorcγt) encoded by the targeted allele are not detected in the thymus. EGFP expression reports Rorc(γt) transcription in the thymi of adult Rorc(γt)GFP mice. Homozygous mice exhibit abnormal lymph node, Peyer's patch, and lymphoid tissue inducer (LTi) cell development. Mice with Rorcγt-deficient T cells lack tissue-infiltrating proinflammatory T-helper cells (Th17 cells), and are protected from induced autoimmune disease (EAE) on this genetic background. The donating investigator also reports increased thymoma incidence with age in homozygotes. These RorcγtGFP mutant mice may be useful in studying immune system homeostasis, T cell repertoire selection, CD4/CD8 double positive (CD4+/CD8+) thymocyte survival, lymphoid organogenesis, proinflammatory T-helper cell (Th17) development, mucosal immunology, and the role of inflammatory disease in autoimmunity and cancer progression.Development
A targeting vector was designed to insert the coding sequence of Enhanced Green Fluorescent Protein (EGFP), followed by a loxP-flanked neomycin resistance cassette, into "exon 1γt" directly downstream of the ATG translational start codon of the targeted gene. The construct was electroporated into the 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts, and chimeras were bred to unspecified mice to generate Rorc(γt)GFPneo heterozygotes. These mice were then crossed with a Cre-deleter strain (unspecified genetic background) to excise the neo cassette. The donating investigator reported that the resulting Rorc(γt)GFP pups were then backcrossed to C57BL/6 (see SNP note below) for at least five generations prior to arrival at The Jackson Laboratory.A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, at least 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
008771 B6(Cg)-Rorctm3Litt/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Rorctm2Litt/Rorctm2Litt
involves: 129P2/OlaHsd
- hematopoietic system phenotype
- abnormal CD4-positive, alpha-beta intraepithelial T cell morphology
- numbers of intestinal alpha beta T cells including CD4+ cells are significantly reduced (MGI Ref ID J:91566)
- abnormal CD8 positive, alpha-beta intraepithelial T cell morphology
- numbers of intestinal alpha beta T cells including CD8alpha-beta+ and CD8alpha-alpha+ cells are significantly reduced (MGI Ref ID J:91566)
- decreased NK cell number
- decreased double-negative T cell number
- numbers of intestinal alpha beta T cells including CD4-CD8- cells are significantly reduced (MGI Ref ID J:91566)
- decreased double-positive T cell number
- fewer double-positive T cells (30-50% that of wild-type) (MGI Ref ID J:87395)
- increased thymocyte apoptosis
- isolated thymocytes undergo apoptosis in culture within 2-3 hours compared to controls that survive for more than 5 hours (MGI Ref ID J:87395)
- immune system phenotype
- abnormal CD4-positive, alpha-beta intraepithelial T cell morphology
- numbers of intestinal alpha beta T cells including CD4+ cells are significantly reduced (MGI Ref ID J:91566)
- abnormal CD8 positive, alpha-beta intraepithelial T cell morphology
- numbers of intestinal alpha beta T cells including CD8alpha-beta+ and CD8alpha-alpha+ cells are significantly reduced (MGI Ref ID J:91566)
- abnormal gut-associated lymphoid tissue morphology
- absent Peyer's patches (MGI Ref ID J:87395)
- abnormal lymph organ development
- absence of lymphoid tissue inducer (LTi) cells (MGI Ref ID J:87395)
- absent lymph nodes
- mice lack all lymph nodes (MGI Ref ID J:87395)
- decreased NK cell number
- decreased double-negative T cell number
- numbers of intestinal alpha beta T cells including CD4-CD8- cells are significantly reduced (MGI Ref ID J:91566)
- decreased double-positive T cell number
- fewer double-positive T cells (30-50% that of wild-type) (MGI Ref ID J:87395)
- increased thymocyte apoptosis
- isolated thymocytes undergo apoptosis in culture within 2-3 hours compared to controls that survive for more than 5 hours (MGI Ref ID J:87395)
- cellular phenotype
- decreased double-negative T cell number
- numbers of intestinal alpha beta T cells including CD4-CD8- cells are significantly reduced (MGI Ref ID J:91566)
- decreased double-positive T cell number
- fewer double-positive T cells (30-50% that of wild-type) (MGI Ref ID J:87395)
- increased thymocyte apoptosis
- isolated thymocytes undergo apoptosis in culture within 2-3 hours compared to controls that survive for more than 5 hours (MGI Ref ID J:87395)
This mouse can be used to support research in many areas including:
GFP relatedApoptosis Research
Endogenous Regulators
Cancer Research
Growth Factors/Receptors/Cytokines
Developmental Biology Research
Internal/Organ Defects
Lymphoid Tissue Defects
Lymphoid Tissue Defects
hematopoietic defects
Immunology, Inflammation and Autoimmunity Research
Autoimmunity
Growth Factors/Receptors/Cytokines
Immunodeficiency
specific T cell deficiency
Lymphoid Tissue Defects
hematopoietic development
selective lymph node development defects
Internal/Organ Research
Gastrointestinal Defects
Lymphoid Tissue Defects
T cell deficiency
Thymus Defects
Research Tools
Apoptosis Research
Developmental Biology Research
Fluorescent Proteins
Immunology and Inflammation Research
specific T cell deficiency
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Rorctm2Litt | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Dan R Littman | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | RORgammat; Roc(gt)gfp; Rorc(gammat)-; Rorc(gammat)GFP; RorcGFP; | ||
| Mutation Made By | Dr. Dan Littman, New York University Medical Center | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | EGFP expression is reported in the thymus (cortex) of adult mice. EGFP is expressed in double-positive thymocytes, with small amounts of EGFP in CD4+ and CD8+ single-positive thymocytes. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Molecular Note | An isoform-specific disruption was generated by inserting a cassette containing eGFP downstream of the start codon of exon 1gammat. The targeted exon is the third exon of the gene, serves as an alternative first exon for the gammat transcript, and is notincluded in the gamma transcript. RT-PCR analysis of hepatic RNA obtained from homozygous mutant mice identified gamma transcript but not the targted gammat transcript. The eGFP sequence included a stop codon but no splice sites or polyadenylation signals. A floxed neo cassette included in the targeting vector was excised from the germline via cre-mediated recombination. [MGI Ref ID J:87395] | ||
| Gene Symbol and Name | Rorc, RAR-related orphan receptor gamma | ||
| Chromosome | 3 | ||
| Gene Common Name(s) | NR1F3; RORG; RORgamma; RZR-GAMMA; RZRG; TOR; Thor; thymus orphan receptor; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Rorctm2Litt, Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Rorctm2Litt, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Eberl G; Marmon S; Sunshine MJ; Rennert PD; Choi Y; Littman DR. 2004. An essential function for the nuclear receptor RORgamma(t) in the generation of fetal lymphoid tissue inducer cells. Nat Immunol 5(1):64-73. [PubMed: 14691482] [MGI Ref ID J:87395]
Ivanov II; McKenzie BS; Zhou L; Tadokoro CE; Lepelley A; Lafaille JJ; Cua DJ; Littman DR. 2006. The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells. Cell 126(6):1121-33. [PubMed: 16990136] [MGI Ref ID J:115922]
Additional References
Rorctm2Litt relatedAliahmad P; de la Torre B; Kaye J. 2010. Shared dependence on the DNA-binding factor TOX for the development of lymphoid tissue-inducer cell and NK cell lineages. Nat Immunol 11(10):945-52. [PubMed: 20818394] [MGI Ref ID J:164687]
Chalmin F; Mignot G; Bruchard M; Chevriaux A; Vegran F; Hichami A; Ladoire S; Derangere V; Vincent J; Masson D; Robson SC; Eberl G; Pallandre JR; Borg C; Ryffel B; Apetoh L; Rebe C; Ghiringhelli F. 2012. Stat3 and Gfi-1 transcription factors control Th17 cell immunosuppressive activity via the regulation of ectonucleotidase expression. Immunity 36(3):362-73. [PubMed: 22406269] [MGI Ref ID J:187139]
Ciofani M; Madar A; Galan C; Sellars M; Mace K; Pauli F; Agarwal A; Huang W; Parkurst CN; Muratet M; Newberry KM; Meadows S; Greenfield A; Yang Y; Jain P; Kirigin FK; Birchmeier C; Wagner EF; Murphy KM; Myers RM; Bonneau R; Littman DR. 2012. A validated regulatory network for th17 cell specification. Cell 151(2):289-303. [PubMed: 23021777] [MGI Ref ID J:188993]
Cui G; Qin X; Wu L; Zhang Y; Sheng X; Yu Q; Sheng H; Xi B; Zhang JZ; Zang YQ. 2011. Liver X receptor (LXR) mediates negative regulation of mouse and human Th17 differentiation. J Clin Invest 121(2):658-70. [PubMed: 21266776] [MGI Ref ID J:171827]
Dang EV; Barbi J; Yang HY; Jinasena D; Yu H; Zheng Y; Bordman Z; Fu J; Kim Y; Yen HR; Luo W; Zeller K; Shimoda L; Topalian SL; Semenza GL; Dang CV; Pardoll DM; Pan F. 2011. Control of T(H)17/T(reg) Balance by Hypoxia-Inducible Factor 1. Cell 146(5):772-84. [PubMed: 21871655] [MGI Ref ID J:176230]
Eberl G; Littman DR. 2004. Thymic origin of intestinal alphabeta T Cells Revealed by Fate Mapping of RORgammat+ Cells. Science 305(5681):248-51. [PubMed: 15247480] [MGI Ref ID J:91566]
Egawa T; Eberl G; Taniuchi I; Benlagha K; Geissmann F; Hennighausen L; Bendelac A; Littman DR. 2005. Genetic evidence supporting selection of the valpha14i NKT cell lineage from double-positive thymocyte precursors. Immunity 22(6):705-16. [PubMed: 15963785] [MGI Ref ID J:99111]
Eisenring M; vom Berg J; Kristiansen G; Saller E; Becher B. 2010. IL-12 initiates tumor rejection via lymphoid tissue-inducer cells bearing the natural cytotoxicity receptor NKp46. Nat Immunol 11(11):1030-8. [PubMed: 20935648] [MGI Ref ID J:166535]
Haas JD; Nistala K; Petermann F; Saran N; Chennupati V; Schmitz S; Korn T; Wedderburn LR; Forster R; Krueger A; Prinz I. 2011. Expression of miRNAs miR-133b and miR-206 in the Il17a/f Locus Is Co-Regulated with IL-17 Production in alphabeta and gammadelta T Cells. PLoS One 6(5):e20171. [PubMed: 21637854] [MGI Ref ID J:172563]
Halim TY; Maclaren A; Romanish MT; Gold MJ; McNagny KM; Takei F. 2012. Retinoic-Acid-receptor-related orphan nuclear receptor alpha is required for natural helper cell development and allergic inflammation. Immunity 37(3):463-74. [PubMed: 22981535] [MGI Ref ID J:187662]
Hirota K; Turner JE; Villa M; Duarte JH; Demengeot J; Steinmetz OM; Stockinger B. 2013. Plasticity of TH17 cells in Peyer's patches is responsible for the induction of T cell-dependent IgA responses. Nat Immunol 14(4):372-9. [PubMed: 23475182] [MGI Ref ID J:194909]
Hoyler T; Klose CS; Souabni A; Turqueti-Neves A; Pfeifer D; Rawlins EL; Voehringer D; Busslinger M; Diefenbach A. 2012. The Transcription Factor GATA-3 Controls Cell Fate and Maintenance of Type 2 Innate Lymphoid Cells. Immunity 37(4):634-48. [PubMed: 23063333] [MGI Ref ID J:188553]
Kaplan MH; Glosson NL; Stritesky GL; Yeh N; Kinzfogl J; Rohrabaugh SL; Goswami R; Pham D; Levy DE; Brutkiewicz RR; Blum JS; Cooper S; Hangoc G; Broxmeyer HE. 2011. STAT3-dependent IL-21 production from T helper cells regulates hematopoietic progenitor cell homeostasis. Blood 117(23):6198-201. [PubMed: 21505191] [MGI Ref ID J:174700]
Kim BS; Siracusa MC; Saenz SA; Noti M; Monticelli LA; Sonnenberg GF; Hepworth MR; Van Voorhees AS; Comeau MR; Artis D. 2013. TSLP elicits IL-33-independent innate lymphoid cell responses to promote skin inflammation. Sci Transl Med 5(170):170ra16. [PubMed: 23363980] [MGI Ref ID J:194548]
Kimura K; Kanai T; Hayashi A; Mikami Y; Sujino T; Mizuno S; Handa T; Matsuoka K; Hisamatsu T; Sato T; Hibi T. 2012. Dysregulated balance of retinoid-related orphan receptor gammat-dependent innate lymphoid cells is involved in the pathogenesis of chronic DSS-induced colitis. Biochem Biophys Res Commun 427(4):694-700. [PubMed: 23022186] [MGI Ref ID J:190311]
Kiss EA; Vonarbourg C; Kopfmann S; Hobeika E; Finke D; Esser C; Diefenbach A. 2011. Natural aryl hydrocarbon receptor ligands control organogenesis of intestinal lymphoid follicles. Science 334(6062):1561-5. [PubMed: 22033518] [MGI Ref ID J:179017]
Klose CS; Kiss EA; Schwierzeck V; Ebert K; Hoyler T; d'Hargues Y; Goppert N; Croxford AL; Waisman A; Tanriver Y; Diefenbach A. 2013. A T-bet gradient controls the fate and function of CCR6-RORgammat+ innate lymphoid cells. Nature 494(7436):261-5. [PubMed: 23334414] [MGI Ref ID J:194555]
Krautler NJ; Kana V; Kranich J; Tian Y; Perera D; Lemm D; Schwarz P; Armulik A; Browning JL; Tallquist M; Buch T; Oliveira-Martins JB; Zhu C; Hermann M; Wagner U; Brink R; Heikenwalder M; Aguzzi A. 2012. Follicular dendritic cells emerge from ubiquitous perivascular precursors. Cell 150(1):194-206. [PubMed: 22770220] [MGI Ref ID J:186239]
Kupz A; Scott TA; Belz GT; Andrews DM; Greyer M; Lew AM; Brooks AG; Smyth MJ; Curtiss R 3rd; Bedoui S; Strugnell RA. 2013. Contribution of Thy1+ NK cells to protective IFN-gamma production during Salmonella Typhimurium infections. Proc Natl Acad Sci U S A 110(6):2252-7. [PubMed: 23345426] [MGI Ref ID J:194337]
Lee JS; Cella M; McDonald KG; Garlanda C; Kennedy GD; Nukaya M; Mantovani A; Kopan R; Bradfield CA; Newberry RD; Colonna M. 2011. AHR drives the development of gut ILC22 cells and postnatal lymphoid tissues via pathways dependent on and independent of Notch. Nat Immunol 13(2):144-51. [PubMed: 22101730] [MGI Ref ID J:180367]
Lochner M; Ohnmacht C; Presley L; Bruhns P; Si-Tahar M; Sawa S; Eberl G. 2011. Microbiota-induced tertiary lymphoid tissues aggravate inflammatory disease in the absence of RORgamma t and LTi cells. J Exp Med 208(1):125-34. [PubMed: 21173107] [MGI Ref ID J:176859]
Luci C; Reynders A; Ivanov II; Cognet C; Chiche L; Chasson L; Hardwigsen J; Anguiano E; Banchereau J; Chaussabel D; Dalod M; Littman DR; Vivier E; Tomasello E. 2009. Influence of the transcription factor RORgammat on the development of NKp46+ cell populations in gut and skin. Nat Immunol 10(1):75-82. [PubMed: 19029904] [MGI Ref ID J:142347]
Marchesi F; Martin AP; Thirunarayanan N; Devany E; Mayer L; Grisotto MG; Furtado GC; Lira SA. 2009. CXCL13 expression in the gut promotes accumulation of IL-22-producing lymphoid tissue-inducer cells, and formation of isolated lymphoid follicles. Mucosal Immunol 2(6):486-94. [PubMed: 19741597] [MGI Ref ID J:193379]
Moro K; Yamada T; Tanabe M; Takeuchi T; Ikawa T; Kawamoto H; Furusawa J; Ohtani M; Fujii H; Koyasu S. 2010. Innate production of T(H)2 cytokines by adipose tissue-associated c-Kit(+)Sca-1(+) lymphoid cells. Nature 463(7280):540-4. [PubMed: 20023630] [MGI Ref ID J:156764]
Naito T; Shiohara T; Hibi T; Suematsu M; Ishikawa H. 2008. ROR gamma t is dispensable for the development of intestinal mucosal T cells. Mucosal Immunol 1(3):198-207. [PubMed: 19079179] [MGI Ref ID J:191924]
Nowak EC; Weaver CT; Turner H; Begum-Haque S; Becher B; Schreiner B; Coyle AJ; Kasper LH; Noelle RJ. 2009. IL-9 as a mediator of Th17-driven inflammatory disease. J Exp Med 206(8):1653-60. [PubMed: 19596803] [MGI Ref ID J:151590]
Pantelyushin S; Haak S; Ingold B; Kulig P; Heppner FL; Navarini AA; Becher B. 2012. Rorgammat+ innate lymphocytes and gammadelta T cells initiate psoriasiform plaque formation in mice. J Clin Invest 122(6):2252-6. [PubMed: 22546855] [MGI Ref ID J:188299]
Price AE; Reinhardt RL; Liang HE; Locksley RM. 2012. Marking and quantifying IL-17A-producing cells in vivo. PLoS One 7(6):e39750. [PubMed: 22768117] [MGI Ref ID J:187909]
Qiu J; Heller JJ; Guo X; Chen ZM; Fish K; Fu YX; Zhou L. 2012. The Aryl Hydrocarbon Receptor Regulates Gut Immunity through Modulation of Innate Lymphoid Cells. Immunity 36(1):92-104. [PubMed: 22177117] [MGI Ref ID J:180786]
Ribot JC; deBarros A; Pang DJ; Neves JF; Peperzak V; Roberts SJ; Girardi M; Borst J; Hayday AC; Pennington DJ; Silva-Santos B. 2009. CD27 is a thymic determinant of the balance between interferon-gamma- and interleukin 17-producing gammadelta T cell subsets. Nat Immunol 10(4):427-36. [PubMed: 19270712] [MGI Ref ID J:148001]
Sanos SL; Bui VL; Mortha A; Oberle K; Heners C; Johner C; Diefenbach A. 2009. RORgammat and commensal microflora are required for the differentiation of mucosal interleukin 22-producing NKp46+ cells. Nat Immunol 10(1):83-91. [PubMed: 19029903] [MGI Ref ID J:142348]
Satoh-Takayama N; Vosshenrich CA; Lesjean-Pottier S; Sawa S; Lochner M; Rattis F; Mention JJ; Thiam K; Cerf-Bensussan N; Mandelboim O; Eberl G; Di Santo JP. 2008. Microbial flora drives interleukin 22 production in intestinal NKp46+ cells that provide innate mucosal immune defense. Immunity 29(6):958-70. [PubMed: 19084435] [MGI Ref ID J:142654]
Sharma MD; Hou DY; Liu Y; Koni PA; Metz R; Chandler P; Mellor AL; He Y; Munn DH. 2009. Indoleamine 2,3-dioxygenase controls conversion of Foxp3+ Tregs to TH17-like cells in tumor-draining lymph nodes. Blood 113(24):6102-11. [PubMed: 19366986] [MGI Ref ID J:149490]
Shaw MH; Kamada N; Kim YG; Nunez G. 2012. Microbiota-induced IL-1beta, but not IL-6, is critical for the development of steady-state TH17 cells in the intestine. J Exp Med 209(2):251-8. [PubMed: 22291094] [MGI Ref ID J:181869]
Shields JD; Kourtis IC; Tomei AA; Roberts JM; Swartz MA. 2010. Induction of lymphoidlike stroma and immune escape by tumors that express the chemokine CCL21. Science 328(5979):749-52. [PubMed: 20339029] [MGI Ref ID J:159521]
Steinmetz OM; Summers SA; Gan PY; Semple T; Holdsworth SR; Kitching AR. 2011. The Th17-defining transcription factor RORgammat promotes glomerulonephritis. J Am Soc Nephrol 22(3):472-83. [PubMed: 21183590] [MGI Ref ID J:185881]
Tachibana M; Tenno M; Tezuka C; Sugiyama M; Yoshida H; Taniuchi I. 2011. Runx1/Cbf beta2 complexes are required for lymphoid tissue inducer cell differentiation at two developmental stages. J Immunol 186(3):1450-7. [PubMed: 21178013] [MGI Ref ID J:168918]
Tanaka S; Yoshimoto T; Naka T; Nakae S; Iwakura Y; Cua D; Kubo M. 2009. Natural occurring IL-17 producing T cells regulate the initial phase of neutrophil mediated airway responses. J Immunol 183(11):7523-30. [PubMed: 19890042] [MGI Ref ID J:157401]
Tessmer MS; Reilly EC; Brossay L. 2011. Salivary gland NK cells are phenotypically and functionally unique. PLoS Pathog 7(1):e1001254. [PubMed: 21249177] [MGI Ref ID J:172475]
Tsuji M; Komatsu N; Kawamoto S; Suzuki K; Kanagawa O; Honjo T; Hori S; Fagarasan S. 2009. Preferential generation of follicular B helper T cells from Foxp3+ T cells in gut Peyer's patches. Science 323(5920):1488-92. [PubMed: 19286559] [MGI Ref ID J:145906]
Tsuji M; Suzuki K; Kitamura H; Maruya M; Kinoshita K; Ivanov II; Itoh K; Littman DR; Fagarasan S. 2008. Requirement for lymphoid tissue-inducer cells in isolated follicle formation and T cell-independent immunoglobulin a generation in the gut. Immunity 29(2):261-71. [PubMed: 18656387] [MGI Ref ID J:139002]
Vonarbourg C; Mortha A; Bui VL; Hernandez PP; Kiss EA; Hoyler T; Flach M; Bengsch B; Thimme R; Holscher C; Honig M; Pannicke U; Schwarz K; Ware CF; Finke D; Diefenbach A. 2010. Regulated expression of nuclear receptor RORgammat confers distinct functional fates to NK cell receptor-expressing RORgammat(+) innate lymphocytes. Immunity 33(5):736-51. [PubMed: 21093318] [MGI Ref ID J:167005]
Yamashita T; Iwakura T; Matsui K; Kawaguchi H; Obana M; Hayama A; Maeda M; Izumi Y; Komuro I; Ohsugi Y; Fujimoto M; Naka T; Kishimoto T; Nakayama H; Fujio Y. 2011. IL-6-mediated Th17 differentiation through RORgammat is essential for the initiation of experimental autoimmune myocarditis. Cardiovasc Res 91(4):640-8. [PubMed: 21622681] [MGI Ref ID J:191625]
Yu Y; Wang D; Liu C; Kaosaard K; Semple K; Anasetti C; Yu XZ. 2011. Prevention of GVHD while sparing GVL effect by targeting Th1 and Th17 transcription factor T-bet and RORgammat in mice. Blood 118(18):5011-20. [PubMed: 21856864] [MGI Ref ID J:178805]
Zhou L; Ivanov II; Spolski R; Min R; Shenderov K; Egawa T; Levy DE; Leonard WJ; Littman DR. 2007. IL-6 programs T(H)-17 cell differentiation by promoting sequential engagement of the IL-21 and IL-23 pathways. Nat Immunol 8(9):967-74. [PubMed: 17581537] [MGI Ref ID J:124293]
Zhou L; Lopes JE; Chong MM; Ivanov II; Min R; Victora GD; Shen Y; Du J; Rubtsov YP; Rudensky AY; Ziegler SF; Littman DR. 2008. TGF-beta-induced Foxp3 inhibits T(H)17 cell differentiation by antagonizing RORgammat function. Nature 453(7192):236-40. [PubMed: 18368049] [MGI Ref ID J:136164]
Zindl CL; Kim TH; Zeng M; Archambault AS; Grayson MH; Choi K; Schreiber RD; Chaplin DD. 2009. The lymphotoxin LTalpha(1)beta(2) controls postnatal and adult spleen marginal sinus vascular structure and function. Immunity 30(3):408-20. [PubMed: 19303389] [MGI Ref ID J:147033]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous or homozygous mice may be bred. Mating System Heterozygote x Homozygote (Female x Male) 26-JAN-11 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Heterozygous for Rorctm2Litt $232.00 Female or Male Homozygous for Rorctm2Litt
Price per Pair (US dollars $) Pair Genotype $464.00 Heterozygous for Rorctm2Litt x Homozygous for Rorctm2Litt Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Heterozygous for Rorctm2Litt $301.60 Female or Male Homozygous for Rorctm2Litt
Price per Pair (US dollars $) Pair Genotype $603.20 Heterozygous for Rorctm2Litt x Homozygous for Rorctm2Litt Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.