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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain NOD/Necker and NOD/ShiLtJ Donor Strain C57BL/6JOrl H2 Haplotype g7 Generation N10+N5F7p
Generation DefinitionsDonating Investigator T1DR Colony, The Jackson Laboratory Appearance
albino, pink-eyed
Related Genotype: A/A Tyrc/TyrcDescription
Klrb1ca (Nk1.1) homozygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Nk1.1 expression in the strain has been confirmed by FACS analysis of peripheral blood cells. The C57BL/6-derived congenic interval on Chromosome 6 which contains the Nk1.1 locus is shorter in these mice than that carried in the original strain (Stock No. 004482). Diabetes incidence in this strain is higher than that reported in strain 004482 (65% in females and 15% in males by 27 weeks for this strain versus 30% in the females and 0% males by 30 weeks of age for Stock No. 004482). Histological examination of the pancreas from 27-week old, non-diabetic mice reveals extensive insulitis that is not significantly different from that seen in similarly aged Stock No. 004482 mice or NOD/ShiLtJ inbred mice (Stock No. 001976). This model is useful for looking at the role of natural killer cells in autoimmune diseases such as Type 1 Diabetes.Development
A Chromosome 6 genomic segment from C57BL/6JOrl originally reported to span D6Mit254 through D6Mit14 and containing Klrb1ca (commonly referred to as NK1.1) was transferred through 10 generations of backcrossing to NOD/Necker (Carnaud et al, 2001). In 2002, this strain arrived at The Jackson Laboratory at N10F? (Stock No. 004482). A partial genome scan indicated that the C57BL/JOrl congenic interval on Chr. 6 was longer than what was originally published. Stock No. 004482 was further backcrossed to NOD/ShiLtJ (Stock No. 001976) prior to making the stock homozygous for a smaller C57BL/6JOrl-derived interval (D6Mit254-D6Mit289) containing Klrb1ca [NK1.1 (62.1 cM)]. The C57BL/6 genetic segment in this NOD congenic stock is approximately 8.5cM shorter than the original stock received. Microsatelite data comparisons may be viewed on the T1DR web site. In 2007, the generation of this strain is N10+N5F4.
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of D6Mit254
004482 NOD.B6-(D6Mit254-D6Mit339)/CarJ View Strains carrying other alleles of D6Mit254 (1 strain)
004482 NOD.B6-(D6Mit254-D6Mit339)/CarJ
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains
NOD/ShiLtJ Non-MHC Congenics
Immunology and Inflammation Research
Autoimmunity
Type 1 Diabetes
Genes & Alleles
Gene & Allele Information provided by MGI
| Gene Symbol and Name | D6Mit254, DNA segment, Chr 6, Massachusetts Institute of Technology 254 | ||
|---|---|---|---|
| Chromosome | 6 | ||
| Gene Symbol and Name | D6Mit289, DNA segment, Chr 6, Massachusetts Institute of Technology 289 | ||
| Chromosome | 6 | ||
| Gene Symbol and Name | Klrb1c, killer cell lectin-like receptor subfamily B member 1C | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AI462337; CD161; Ly-59; Ly55c; Ly59; NK-1.1; NK-RP1; NKR-P1; NKR-P1C; Nk-1; Nk-1.2; Nk1; Nk1.1; Nkrp1-c; expressed sequence AI462337; lymphocyte antigen 55 complex, locus C; lymphocyte antigen 59; natural killer cell-associated antigen 1; | ||
References
References provided by MGI
Selected Reference(s)
Carnaud C; Gombert J; Donnars O; Garchon H; Herbelin A. 2001. Protection against diabetes and improved NK/NKT cell performance in NOD.NK1.1 mice congenic at the NK complex. J Immunol 166(4):2404-11. [PubMed: 11160299] [MGI Ref ID J:68580]
Poulton LD; Smyth MJ; Hawke CG; Silveira P; Shepherd D; Naidenko OV; Godfrey DI; Baxter AG. 2001. Cytometric and functional analyses of NK and NKT cell deficiencies in NOD mice. Int Immunol 13(7):887-96. [PubMed: 11431419] [MGI Ref ID J:121816]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $1600.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $2080.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Type 1 Diabetes Repository collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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