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| These transgenic mice overexpress neuroglobin (Ngb) and GFP in multiple tissues, and are resistant to ischemia. When crossed to a mouse model of Alzheimer's disease, the disease phenotype is attenuated. They may be useful in studies of cerebral and myocardial ischemia, stroke, and neurodegenerative disease. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N6F1pN1
Generation DefinitionsDonating Investigator IMR Colony, The Jackson Laboratory Description
These transgenic mice express mouse neuroglobin and Enhanced Green Fluorescent Protein under the direction of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) distal enhancer. Western blot analysis detects increased NGB protein in heart brain of homozygotes. Transgenic mice have NGB-overexpressing neurons, astrocytes and endothelial cells in the cerebral cortex. The donating investigator reports that fluorescence is detected in all tissues. Experimentally induced ischemia in transgenic mice results in cerebral infarct volume reduction of approximately 30% and myocardial infarct volume reduction of approximately 25% when compared to wild-type. Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of cerebral (CNS) and myocardial ischemia and stroke.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A transgenic construct containing the mouse neuroglobin gene Ngb and an Enhanced Green Fluorescent Protein gene under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) distal enhancer was introduced into outbred BDF X CD1 donor eggs. Founder line 1 was subsequently established. Hemizygotes were intercrossed to generate homozygotes prior to arrival at The Jackson Laboratory. Upon arrival, some mice were backcrossed to C57BL/6J for at least five generations to generate this congenic strain.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Alzheimer's Disease Models
View Alzheimer's Disease Models (108 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(CAG-Ngb,-EGFP)1Dgrn/Tg(CAG-Ngb,-EGFP)1Dgrn
involves: C57BL/6 * CD-1 * DBA/2
- cardiovascular system phenotype
- decreased myocardial infarction size
- transmural infarct affecting left ventricle, produced by occlusion of left anterior descending coronary artery (LADA), is ~25% smaller (as percentage of left ventricle volume or percentage of LADA territory) than in controls (MGI Ref ID J:117158)
- nervous system phenotype
- decreased cerebral infarction size
- MCA occlusion results in an ipsilateral infarct affecting the striatum and cerebral cortex that is ~30% smaller than the lesion produced in wild-type brains (MGI Ref ID J:117158)
- homeostasis/metabolism phenotype
- abnormal nitric oxide homeostasis
- myocardial tissue sections from transgenic animals show markedly increased endothelial nitric oxide synthase (eNOS) immunoreactivity and increased eNOS protein expression by Western blot compared to wild-type myocardium (MGI Ref ID J:117158)
- decreased cerebral infarction size
- MCA occlusion results in an ipsilateral infarct affecting the striatum and cerebral cortex that is ~30% smaller than the lesion produced in wild-type brains (MGI Ref ID J:117158)
- decreased myocardial infarction size
- transmural infarct affecting left ventricle, produced by occlusion of left anterior descending coronary artery (LADA), is ~25% smaller (as percentage of left ventricle volume or percentage of LADA territory) than in controls (MGI Ref ID J:117158)
This mouse can be used to support research in many areas including:
GFP relatedCardiovascular Research
Ischemia studies
Neurobiology Research
Alzheimer's Disease
Research Tools
Fluorescent Proteins
Neurobiology Research
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(CAG-Ngb,-EGFP)1Dgrn | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, David Greenberg | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Ngb-Tg; Tg(ACTB-Ngb,-EGFP)1Dgrn; Tg(CAG-Ngb,-EGFP)1Dgrn; | ||
| Mutation Made By | Eric Giegerich, Buck Institute for Age Research | ||
| Strain of Origin | (C57BL/6 x DBA/2)F1 x CD-1 | ||
| Site of Expression | Green fluorescence has been reported in all tissues tested | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | A transgenic construct containing the mouse neuroglobin gene, Ngb and an Enhanced Green Fluorescent Protein gene under the control of the chicken beta actin (ACTB) promoter coupled with the cytomegalovirus (CMV) distal enhancer was introduced into outbred (C57BL/6 x DBA/2)F1 x CD-1 donor eggs. Founder line 1 was subsequently established. Constitutive Ngb protein expression is increased in the brain and heart (in vascular endothelial cells) of transgenic mice, as shown by Western blot. Immunohistochemistry shows increased numbers of neurons, astrocytes and endothelial cells expressing Ngb protein. [MGI Ref ID J:117158] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(CAG-Ngb,-EGFP)1Dgrn, QPCR
Tg(CAG-Ngb,-EGFP)1Dgrn, QPCR
Tg(CAG-Ngb,-EGFP)1Dgrn, Standard PCR
Tg(TIE2GFP)287Sato, Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Khan AA; Wang Y; Sun Y; Mao XO; Xie L; Miles E; Graboski J; Chen S; Ellerby LM; Jin K; Greenberg DA. 2006. Neuroglobin-overexpressing transgenic mice are resistant to cerebral and myocardial ischemia. Proc Natl Acad Sci U S A 103(47):17944-8. [PubMed: 17098866] [MGI Ref ID J:117158]
Additional References
Khan AA; Mao XO; Banwait S; Dermardirossian CM; Bokoch GM; Jin K; Greenberg DA. 2008. Regulation of hypoxic neuronal death signaling by neuroglobin. FASEB J 22(6):1737-1747. [PubMed: 18198211] [MGI Ref ID J:135001]
Khan AA; Mao XO; Banwait S; Jin K; Greenberg DA. 2007. Neuroglobin attenuates beta-amyloid neurotoxicity in vitro and transgenic Alzheimer phenotype in vivo. Proc Natl Acad Sci U S A 104(48):19114-9. [PubMed: 18025470] [MGI Ref ID J:127621]
Khan AA; Sun Y; Jin K; Mao XO; Chen S; Ellerby LM; Greenberg DA. 2007. A neuroglobin-overexpressing transgenic mouse. Gene 398(1-2):172-6. [PubMed: 17537594] [MGI Ref ID J:123213]
Tg(CAG-Ngb,-EGFP)1Dgrn relatedYu Z; Liu J; Guo S; Xing C; Fan X; Ning M; Yuan JC; Lo EH; Wang X. 2009. Neuroglobin-overexpression alters hypoxic response gene expression in primary neuron culture following oxygen glucose deprivation. Neuroscience 162(2):396-403. [PubMed: 19401220] [MGI Ref ID J:152932]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry Mutant mice were bred to C57BL/6J mice to generate this congenic strain. When maintaining the live congenic colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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|
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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