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| These transgenic mice overexpress neuroglobin (Ngb) and GFP in multiple tissues, and are resistant to ischemia. When crossed to a mouse model of Alzheimer's disease, the disease phenotype is attenuated. They may be useful in studies of cerebral and myocardial ischemia, stroke, and neurodegenerative disease. | |||||||||||
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Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 03-SEP-08 Species laboratory mouse Generation N6+F1 (15-JAN-09) Donating Investigator IMR Colony, The Jackson Laboratory Description
These transgenic mice express mouse neuroglobin and Enhanced Green Fluorescent Protein under the direction of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) distal enhancer. Western blot analysis detects increased NGB protein in heart brain of homozygotes. Transgenic mice have NGB-overexpressing neurons, astrocytes and endothelial cells in the cerebral cortex. The donating investigator reports that fluorescence is detected in all tissues. Experimentally induced ischemia in transgenic mice results in cerebral infarct volume reduction of approximately 30% and myocardial infarct volume reduction of approximately 25% when compared to wild-type. Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of cerebral (CNS) and myocardial ischemia and stroke.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A transgenic construct containing the mouse neuroglobin gene Ngb and an Enhanced Green Fluorescent Protein gene under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) distal enhancer was introduced into outbred BDF X CD1 donor eggs. Founder line 1 was subsequently established. Hemizygotes were intercrossed to generate homozygotes prior to arrival at The Jackson Laboratory. Upon arrival, some mice were backcrossed to C57BL/6J for at least five generations to generate this congenic strain.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(CAG-Ngb,-EGFP)1Dgrn/Tg(CAG-Ngb,-EGFP)1Dgrn
involves: C57BL/6 * CD-1 * DBA/2
- cardiovascular system phenotype
- decreased infarction size (MGI Ref ID J:117158)
- transmural infarct affecting left ventricle, produced by occlusion of left anterior descending coronary artery (LADA), is ~25% smaller (as percentage of left ventricle volume or percentage of LADA territory) than in controls
- nervous system phenotype
- decreased cerebral infarction size (MGI Ref ID J:117158)
- MCA occlusion results in an ipsilateral infarct affecting the striatum and cerebral cortex that is ~30% smaller than the lesion produced in wild-type brains
- homeostasis/metabolism phenotype
- abnormal nitric oxide homeostasis (MGI Ref ID J:117158)
- myocardial tissue sections from transgenic animals show markedly increased endothelial nitric oxide synthase (eNOS) immunoreactivity and increased eNOS protein expression by Western blot compared to wild-type myocardium
- decreased cerebral infarction size (MGI Ref ID J:117158)
- MCA occlusion results in an ipsilateral infarct affecting the striatum and cerebral cortex that is ~30% smaller than the lesion produced in wild-type brains
- decreased infarction size (MGI Ref ID J:117158)
- transmural infarct affecting left ventricle, produced by occlusion of left anterior descending coronary artery (LADA), is ~25% smaller (as percentage of left ventricle volume or percentage of LADA territory) than in controls
This mouse can be used to support research in many areas including:
GFP relatedCardiovascular Research
Ischemia studies
Neurobiology Research
Alzheimer's Disease
Research Tools
Fluorescent Proteins
Neurobiology Research
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(CAG-Ngb,-EGFP)1Dgrn | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, David Greenberg | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Ngb-Tg; Tg(ACTB-Ngb,-EGFP)1Dgrn; Tg(CAG-Ngb,-EGFP)1Dgrn; | ||
| Mutation Made By | Eric Giegerich, Buck Institute for Age Research | ||
| Strain of Origin | (C57BL/6 x DBA/2)F1 x CD-1 | ||
| Site of Expression | Green fluorescence has been reported in all tissues tested | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | Ngb, neuroglobin, mouse, laboratory | ||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | A transgenic construct containing the mouse neuroglobin gene, Ngb and an Enhanced Green Fluorescent Protein gene under the control of the chicken beta actin (ACTB) promoter coupled with the cytomegalovirus (CMV) distal enhancer was introduced into outbred (C57BL/6 x DBA/2)F1 x CD-1 donor eggs. Founder line 1 was subsequently established. Constitutive Ngb protein expression is increased in the brain and heart (in vascular endothelial cells) of transgenic mice, as shown by Western blot. Immunohistochemistry shows increased numbers of neurons, astrocytes and endothelial cells expressing Ngb protein. [MGI Ref ID J:117158] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(CAG-Ngb,-EGFP)1Dgrn, QPCR
Tg(CAG-Ngb,-EGFP)1Dgrn, QPCR
Tg(CAG-Ngb,-EGFP)1Dgrn, Standard PCR
Tg(TIE2GFP)287Sato, Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Khan AA; Wang Y; Sun Y; Mao XO; Xie L; Miles E; Graboski J; Chen S; Ellerby LM; Jin K; Greenberg DA. 2006. Neuroglobin-overexpressing transgenic mice are resistant to cerebral and myocardial ischemia. Proc Natl Acad Sci U S A 103(47):17944-8. [PubMed: 17098866] [MGI Ref ID J:117158]
Additional References
Khan AA; Mao XO; Banwait S; Dermardirossian CM; Bokoch GM; Jin K; Greenberg DA. 2008. Regulation of hypoxic neuronal death signaling by neuroglobin. FASEB J 22(6):1737-1747. [PubMed: 18198211] [MGI Ref ID J:135001]
Khan AA; Mao XO; Banwait S; Jin K; Greenberg DA. 2007. Neuroglobin attenuates beta-amyloid neurotoxicity in vitro and transgenic Alzheimer phenotype in vivo. Proc Natl Acad Sci U S A 104(48):19114-9. [PubMed: 18025470] [MGI Ref ID J:127621]
Khan AA; Sun Y; Jin K; Mao XO; Chen S; Ellerby LM; Greenberg DA. 2007. A neuroglobin-overexpressing transgenic mouse. Gene 398(1-2):172-6. [PubMed: 17537594] [MGI Ref ID J:123213]
Tg(CAG-Ngb,-EGFP)1Dgrn relatedYu Z; Liu J; Guo S; Xing C; Fan X; Ning M; Yuan JC; Lo EH; Wang X. 2009. Neuroglobin-overexpression alters hypoxic response gene expression in primary neuron culture following oxygen glucose deprivation. Neuroscience 162(2):396-403. [PubMed: 19401220] [MGI Ref ID J:152932]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry Mutant mice were bred to C57BL/6J mice to generate this congenic strain. When maintaining the live congenic colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice. Mating System Noncarrier x Hemizygote (Female x Male) 03-SEP-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(CAG-Ngb,-EGFP)1Dgrn
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(CAG-Ngb,-EGFP)1Dgrn x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(CAG-Ngb,-EGFP)1Dgrn
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(CAG-Ngb,-EGFP)1Dgrn
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(CAG-Ngb,-EGFP)1Dgrn x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(CAG-Ngb,-EGFP)1Dgrn
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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