Strain Name: |
B6.Cg-Tg(CAG-Ngb,-EGFP)1Dgrn/J |
|---|---|
Stock Number: |
007575 |
Availability: | Under Development for Distribution Colony |
| To register your interest in this strain go to the Strain Interest Form. |
General Terms and Conditions |
| Genes & Alleles | ACTB; GFP; Ngp; Tg(CAG-Ngb,-EGFP)1Dgrn; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation N3 (06-MAY-08) Strain Description
These transgenic mice express mouse neuroglobin and Enhanced Green Fluorescent Protein under the direction of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) distal enhancer. Western blot analysis detects increased NGB protein in heart brain of homozygotes. Transgenic mice have NGB-overexpressing neurons, astrocytes and endothelial cells in the cerebral cortex. The donating investigator reports that fluorescence is detected in all tissues. Experimentally induced ischemia in transgenic mice results in cerebral infarct volume reduction of approximately 30% and myocardial infarct volume reduction of approximately 25% when compared to wildtype. Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of cerebral (CNS) and myocardial ischemia and stroke.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A transgenic construct containing the mouse neuroglobin gene Ngb and an Enhanced Green Fluorescent Protein gene under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) distal enhancer was introduced into outbred BDF X CD1 donor eggs. Founder line 1 was subsequently established. Hemizygotes were intercrossed to generate homozygotes prior to arrival at The Jackson Laboratory. Upon arrival, some mice were backcrossed to C57BL/6J for at least 5 generations to generate this congenic strain.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(CAG-Ngb,-EGFP)1Dgrn | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, David Greenberg | ||
| Common Name(s) | Ngb-Tg; Tg(ACTB-Ngb,-EGFP)1Dgrn; Tg(CAG-Ngb,-EGFP)1Dgrn; | ||
| Mutation Made By | Eric Giegerich, Buck Institute for Age Research | ||
| Strain of Origin | (C57BL/6 x DBA/2)F1 x CD-1 | ||
| Site of Expression | Green fluorescence has been reported in all tissues tested | ||
| Expressed Gene | Ngp, neutrophilic granule protein, mouse, laboratory | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | A transgenic construct containing the mouse neuroglobin gene, Ngb and an Enhanced Green Fluorescent Protein gene under the control of the chicken beta actin (ACTB) promoter coupled with the cytomegalovirus (CMV) distal enhancer was introduced into outbred (C57BL/6 x DBA/2)F1 x CD-1 donor eggs. Founder line 1 was subsequently established. Constitutive Ngb protein expression is increased in the brain and heart (in vascular endothelial cells) of transgenic mice, as shown by Western blot. Immunohistochemistry shows increased numbers of neurons, astrocytes and endothelial cells expressing Ngb protein. [MGI Ref ID J:117158] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(CAG-Ngb,-EGFP)1Dgrn
Tg(CAG-Ngb,-EGFP)1Dgrn QPCR
Tg(CAG-Ngb,-EGFP)1Dgrn QPCR
Colony Maintenance
| Breeding & Husbandry | Mutant mice were bred to C57BL/6J mice to generate this congenic strain. When maintaining the live congenic colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of ACTB
View Strains carrying other alleles of ACTB (31 strains)
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedCardiovascular Research
Ischemia studies
Neurobiology Research
Alzheimer's Disease
Research Tools
Fluorescent Proteins
Neurobiology Research
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesKhan AA; Wang Y; Sun Y; Mao XO; Xie L; Miles E; Graboski J; Chen S; Ellerby LM; Jin K; Greenberg DA. 2006. Neuroglobin-overexpressing transgenic mice are resistant to cerebral and myocardial ischemia. Proc Natl Acad Sci U S A 103(47):17944-8. [PubMed: 17098866] [MGI Ref ID J:117158]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(CAG-Ngb,-EGFP)1Dgrn/J |
| Stock Number: | 007575 |
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 29-SEP-08
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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