| |||||||||||||||
| These mT/mG mice are useful as a Cre reporter strain; expressing red fluorescence prior to, and green fluorescence following, Cre-mediated recombination in widespread cell and tissue types. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 09-APR-08 Species laboratory mouse Generation ?+1F5 (18-NOV-09) Donating Investigator Liqun Luo, Stanford University, HHMI Description
Mice homozygous for this mT/mG mutation are viable and fertile. These mice possess loxP sites on either side of a membrane-targeted tdTomato (mT) cassette and express strong red fluorescence in all tissues and cell types examined. Tail or whole body epifluorescence is sufficient to identify mT/mG homozygotes. When bred to Cre recombinase expressing mice, the resulting offspring have the mT cassette deleted in the cre expressing tissue(s), allowing expression of the membrane-targeted EGFP (mG) cassette located just downstream. The donating investigator reports that the ACTB promoter allows stronger and persistent expression of the fluorescent proteins (especially in adult cells) compared to the endogenous Gt(ROSA) locus alone. This double-fluorescent system allows direct live visualization of both recombined and non-recombined cells at single cell resolution, offering an internal control for phenotypic analysis of Cre-induced mosaic mutants and providing a second marker for lineage tracing applications. In addition, the localization of fluorescent proteins to membrane structures outlines cell morphology and allows resolution of fine cellular processes. These mT/mG mice are useful as a Cre reporter strain; expressing red fluorescence prior to, and green fluorescence following, Cre-mediated recombination in widespread cell and tissue types.Development
The mT/mG (membrane-Tomato/membrane-Green) targeting vector was designed with a CMV enhancer/chicken beta-actin core promoter (pCA) driving expression of a loxP-flanked, N-terminal membrane-tagged, tdTomato protein sequence followed by a polyadenylation signal (tdTomato is a non-oligomerizing DsRed variant with a 12 residue linker fusing two copies of the protein (tandem dimer)). Immediately distal to the second loxP site is an N-terminal membrane-tagged, enhanced green fluorescent protein (EGFP) sequence itself followed by a polyadenylation signal. An frt-flanked neo cassette was also located distal to the expression vector. This entire mT/mG construct was inserted into the Gt(ROSA)26Sor locus via electroporation of (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were microinjected in C57BL/6J blastocysts. Chimeric progeny were bred to outbred CD-1 mice. The resulting mutant mice were interbred to generate homozygotes prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to 129X1/SvJ (Stock No. 000691) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| 000691 129X1/SvJ | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
007676 B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
006067 129-Gt(ROSA)26Sortm2Luo/J 006041 129-Gt(ROSA)26Sortm3Luo/J 006080 B6.129-Gt(ROSA)26Sortm2Luo/J 006075 B6.129-Gt(ROSA)26Sortm3Luo/J 005884 B6.Cg-Tg(CAG-mRFP1)1F1Hadj/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 005645 STOCK Tg(CAG-mRFP1)1F1Hadj/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo
involves: 129S1/Sv * 129X1/SvJ
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:124702)
- mice are viable and fertile, with no observable adverse phenotypes
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Cre-lox System
loxP-flanked Sequences: Test/Reporter
Research Tools
Cre-lox System
loxP-flanked Sequences: Test/Reporter
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: multiple
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo | ||
|---|---|---|---|
| Allele Name | targeted mutation 4, Liqun Luo | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | mT/mG; | ||
| Mutation Made By | Liqun Luo, Stanford University, HHMI | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | Membrane-targeted tdTomato (mT) is expressed in all tissues and cell types examined. When bred to Cre recombinase expressing mice, the mT cassette is deleted in the cre expressing tissue(s), allowing expression of the membrane-targeted EGFP (mG). | ||
| Expressed Gene | RFP, Red Fluorescent Protein, jelly fish | ||
| Red Fluorescent Protein (RFP), derived from marine invertebrate organisms such as the soft coral Discosoma spp and reef coral, Heteractis crispa, is a versatile reporter molecule which has found use in many biological applications. The wild type protein, which is an obligate tetramer, is not well tolerated in mammalian systems. The original molecule has been modified in order to optimize expression to mammalian physiology (examples include monomeric RFP, mRFP1, DsRed, etc). | |||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | The targeting vector was designed with a CMV enhancer/chicken beta-actin core promoter (pCA) driving expression of a loxP-flanked, N-terminal membrane-tagged, optimized DsRed fluorescent protein variant (called tandem-dimer-Tomato or tdTomato) sequence followed by a polyadenylation signal. Immediately distal to the second loxP site is an N-terminal membrane-tagged, enhanced green fluorescent protein (EGFP) sequence itself followed by a polyadenylation signal. An frt-flanked neo cassette was also located distal to the expression vector. Red fluorescence is detected in all tissues tested. When mice are bred to Cre expressing mice, the floxed region is excised in cCre-expressing tissue and this allows expression of the EGFP cassette. [MGI Ref ID J:123053] [MGI Ref ID J:124702] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sor(Luo), Melt Curve Analysis
Gt(ROSA)26Sortm1Luo, tm2Luo, tm3Luo, tm4ACTB-tdTomato,-EGFP)Luo, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Muzumdar MD; Tasic B; Miyamichi K; Li L; Luo L. 2007. A global double-fluorescent Cre reporter mouse. Genesis 45(9):593-605. [PubMed: 17868096] [MGI Ref ID J:124702]
Additional References
Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo relatedRock JR; Onaitis MW; Rawlins EL; Lu Y; Clark CP; Xue Y; Randell SH; Hogan BL. 2009. Basal cells as stem cells of the mouse trachea and human airway epithelium. Proc Natl Acad Sci U S A 106(31):12771-5. [PubMed: 19625615] [MGI Ref ID J:152012]
Shaner NC; Campbell RE; Steinbach PA; Giepmans BN; Palmer AE; Tsien RY. 2004. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol 22(12):1567-72. [PubMed: 15558047] [MGI Ref ID J:123053]
Suvorova ES; Lucas O; Weisend CM; Rollins MF; Merrill GF; Capecchi MR; Schmidt EE. 2009. Cytoprotective Nrf2 pathway is induced in chronically txnrd 1-deficient hepatocytes. PLoS One 4(7):e6158. [PubMed: 19584930] [MGI Ref ID J:151615]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as homozygotes. Mating System Homozygote x Homozygote (Female x Male) 09-APR-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $160.40 Female or Male Homozygous for Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo
Pairs /Price (US dollars $) Pair Genotype $320.80 Homozygous for Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo x Homozygous for Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $208.60 Female or Male Homozygous for Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo
Pairs /Price (US dollars $) Pair Genotype $417.10 Homozygous for Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo x Homozygous for Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo
Additional Supply Details
|
|
|
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| 000691 129X1/SvJ | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
- Strain(s) not available to companies or for-profit entities.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.